A permanent magnet linear synchronous motor （PMLSM） for a high temperature superconducting （HTS） maglev system has been studied, including the motor structure, control strategy, and analysis techniques. Finite ele...A permanent magnet linear synchronous motor （PMLSM） for a high temperature superconducting （HTS） maglev system has been studied, including the motor structure, control strategy, and analysis techniques. Finite element analysis （FEA） of magnetic field is conducted to accurately calculate major motor parameters. Equivalent electrical circuit is used to predict the drive＇s steady-state characteristics, and a phase variable model is applied to predict the dynamic performance. Preliminary experiment with a prototype has been made to verify the theoretical analysis and the HTS-PM synchronous driving technology.展开更多
Exploration of deep mineralization,particularly where the mineralization of interest is covered by a conductive overburden,is still a challenge for the conventional transient electromagnetic(TEM)method,which measures ...Exploration of deep mineralization,particularly where the mineralization of interest is covered by a conductive overburden,is still a challenge for the conventional transient electromagnetic(TEM)method,which measures TEM response using induction coils as the sensor.However,sensors such as fluxgate and superconductive quantum interfere device(SQUID)magnetometers can measure the B-field directly,which can provide more reliable deep information for mineralization exploration.In this paper,we report on the research and development of our newly developed high-temperature su-perconductor(HTS)SQUID magnetometer,which is cooled by liquid nitrogen at 77 K,and its applica-tion in TEM measurement for deep exploration in a gold deposit in China.This improved SQUID magnetometer version has a good performance with noise(60 fT/√Hz),slew rate(0.8 mT/S),dynamic range(100 dB),sensitivity(6.25 mV/nT),and bandwidth(DC-20 kHz).To find deep and peripheral ore in the Baiyun gold deposit located in Liaoning Province,NE China,both the SQUID magnetometer and induction coil were used for TEM data acquisition.Results show that TEM can detect the distribution of local strata and the faults contained within them.Results also indicate that the SQUID magnetome-ter has superior response performance for response over geological targets with slower decay time when compared to the induction coil signals.The SQUID magnetometer is more sensitive at observing the induced-polarization effect which is closely related to the ore-controlling faults.展开更多
Ubiquitin-proteasome system(UPS)plays an important role in neurodegenerative diseases,such as Alzheimer’s disease(AD),Parkinson’s disease(PD),and Huntington’s disease(HD).The discovery of UPS activators for anti-ne...Ubiquitin-proteasome system(UPS)plays an important role in neurodegenerative diseases,such as Alzheimer’s disease(AD),Parkinson’s disease(PD),and Huntington’s disease(HD).The discovery of UPS activators for anti-neurodegenerative diseases is becoming increasingly important.In this study,we aimed to identify potential UPS activators using the high-throughput screening method with the high-content fluorescence imaging system and validate the neuroprotective effect in the cell models of AD.At first,stable YFP-CL1 HT22 cells were successfully constructed by transfecting the YFP-CL1 plasmid into HT22 cells,together with G418 screening.The degradation activity of the test compounds via UPS was monitored by detecting the YFP fluorescence intensity reflected by the ubiquitin-proteasome degradation signal CL1.By employing the high-content fluorescence imaging system,together with stable YFP-CL1 HT22 cells,the UPS activators were successfully screened from our established TCM library.The representative images were captured and analyzed,and quantification of the YFP fluorescence intensity was performed by flow cytometry.Then,the neuroprotective effect of the UPS activators was investigated in pEGFP-N1-APP(APP),pRK5-EGFP-Tau P301L(Tau P301L),or pRK5-EGFP-Tau(Tau)transiently transfected HT22 cells using fluorescence imaging,flow cytometry,and Western blot.In conclusion,our study established a high-content fluorescence imaging system coupled with stable YFP-CL1 HT22 cells for the highthroughput screening of the UPS activators.Three compounds,namely salvianolic acid A(SAA),salvianolic acid B(SAB),and ellagic acid(EA),were identified to significantly decrease YFP fluorescence intensity,which suggested that these three compounds are UPS activators.The identified UPS activators were demonstrated to clear AD-related proteins,including APP,Tau,and Tau P301L.Therefore,these findings provide a novel insight into the discovery and development of anti-AD drugs.展开更多
文摘A permanent magnet linear synchronous motor （PMLSM） for a high temperature superconducting （HTS） maglev system has been studied, including the motor structure, control strategy, and analysis techniques. Finite element analysis （FEA） of magnetic field is conducted to accurately calculate major motor parameters. Equivalent electrical circuit is used to predict the drive＇s steady-state characteristics, and a phase variable model is applied to predict the dynamic performance. Preliminary experiment with a prototype has been made to verify the theoretical analysis and the HTS-PM synchronous driving technology.
基金This study was supported by the National Key Research and Development Project of China(No.2018YFC0603803)Fundamental Research Funds of Chinese Academy of Geological Sciences(Nos.AS2020Y01,AS2020P01).
文摘Exploration of deep mineralization,particularly where the mineralization of interest is covered by a conductive overburden,is still a challenge for the conventional transient electromagnetic(TEM)method,which measures TEM response using induction coils as the sensor.However,sensors such as fluxgate and superconductive quantum interfere device(SQUID)magnetometers can measure the B-field directly,which can provide more reliable deep information for mineralization exploration.In this paper,we report on the research and development of our newly developed high-temperature su-perconductor(HTS)SQUID magnetometer,which is cooled by liquid nitrogen at 77 K,and its applica-tion in TEM measurement for deep exploration in a gold deposit in China.This improved SQUID magnetometer version has a good performance with noise(60 fT/√Hz),slew rate(0.8 mT/S),dynamic range(100 dB),sensitivity(6.25 mV/nT),and bandwidth(DC-20 kHz).To find deep and peripheral ore in the Baiyun gold deposit located in Liaoning Province,NE China,both the SQUID magnetometer and induction coil were used for TEM data acquisition.Results show that TEM can detect the distribution of local strata and the faults contained within them.Results also indicate that the SQUID magnetome-ter has superior response performance for response over geological targets with slower decay time when compared to the induction coil signals.The SQUID magnetometer is more sensitive at observing the induced-polarization effect which is closely related to the ore-controlling faults.
基金the Joint Project of Luzhou Municipal People’s Government and Southwest Medical University(No.2020LZXNYDJ37)the National Natural Science Foundation of China(Nos.81903829 and 81801398)+2 种基金the Science and Technology Planning Project of Sichuan Province(Nos.2019JDPT0010 and 2020YJ0494)the Project of Southwest Medical University(Nos.2021ZKZD015,2021ZKZD018,and 2021-ZKMS046)Sichuan University Student Innovation and Entrepreneurship Project(Nos.2019424 and 201816032066).
文摘Ubiquitin-proteasome system(UPS)plays an important role in neurodegenerative diseases,such as Alzheimer’s disease(AD),Parkinson’s disease(PD),and Huntington’s disease(HD).The discovery of UPS activators for anti-neurodegenerative diseases is becoming increasingly important.In this study,we aimed to identify potential UPS activators using the high-throughput screening method with the high-content fluorescence imaging system and validate the neuroprotective effect in the cell models of AD.At first,stable YFP-CL1 HT22 cells were successfully constructed by transfecting the YFP-CL1 plasmid into HT22 cells,together with G418 screening.The degradation activity of the test compounds via UPS was monitored by detecting the YFP fluorescence intensity reflected by the ubiquitin-proteasome degradation signal CL1.By employing the high-content fluorescence imaging system,together with stable YFP-CL1 HT22 cells,the UPS activators were successfully screened from our established TCM library.The representative images were captured and analyzed,and quantification of the YFP fluorescence intensity was performed by flow cytometry.Then,the neuroprotective effect of the UPS activators was investigated in pEGFP-N1-APP(APP),pRK5-EGFP-Tau P301L(Tau P301L),or pRK5-EGFP-Tau(Tau)transiently transfected HT22 cells using fluorescence imaging,flow cytometry,and Western blot.In conclusion,our study established a high-content fluorescence imaging system coupled with stable YFP-CL1 HT22 cells for the highthroughput screening of the UPS activators.Three compounds,namely salvianolic acid A(SAA),salvianolic acid B(SAB),and ellagic acid(EA),were identified to significantly decrease YFP fluorescence intensity,which suggested that these three compounds are UPS activators.The identified UPS activators were demonstrated to clear AD-related proteins,including APP,Tau,and Tau P301L.Therefore,these findings provide a novel insight into the discovery and development of anti-AD drugs.
