Objective： Detecting the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudoge...Objective： Detecting the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify ifhTRF1 mutation is one of the factors of the activation of telomerase. Methods： hTRFlcDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of h TRFlmRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-I, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification （TRAP）-ELISA protocol. PCR and sequencing were used to detect mutation of each exon ofhTRF1 in 10 cell line cells. Results： hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes ofhTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRFI-13, ψhTRFI-18, ψhTRF1-21 and ψhTRFI-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells （0.0338, 0.0108-0.0749） than in normal mononuclear cells （0.0493, 0.0369-0.128） （P=0.004）. But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part ofintron 1, 2 and 8 ofhTRF1. Their infection on gene function is unknown and needs further studies. Conclusion： hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.展开更多
In this paper,we investigate the multiple attribute decision making(MADM)problems in which the attribute values take the form of hesitant trapezoid fuzzy information.Firstly,inspired by the idea of hesitant fuzzy sets...In this paper,we investigate the multiple attribute decision making(MADM)problems in which the attribute values take the form of hesitant trapezoid fuzzy information.Firstly,inspired by the idea of hesitant fuzzy sets and trapezoid fuzzy numbers,the definition of hesitant trapezoid fuzzy set and some operational laws of hesitant trapezoid fuzzy elements are proposed.Then some hesitant trapezoid fuzzy aggregation operators based on Hamacher operation are developed,such as the hesitant trapezoid fuzzy Hamacher weighted average(HTr FHWA)operator,the hesitant trapezoid fuzzy Hamacher weighted geometric(HTr FHWG)operator,the hesitant trapezoid fuzzy Hamacher Choquet average(HTr FHCA),the hesitant trapezoid fuzzy Hamacher Choquet geometric(HTr FHCG),etc.Furthermore,an approach based on the hesitant trapezoid fuzzy Hamacher weighted average(HTr FHWA)operator and the hesitant trapezoid fuzzy Hamacher weighted geometric(HTr FHWG)operator is proposed for MADM problems under hesitant trapezoid fuzzy environment.Finally,a numerical example for supplier selection is given to illustrate the application of the proposed approach.展开更多
文摘Objective： Detecting the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify ifhTRF1 mutation is one of the factors of the activation of telomerase. Methods： hTRFlcDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of h TRFlmRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-I, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification （TRAP）-ELISA protocol. PCR and sequencing were used to detect mutation of each exon ofhTRF1 in 10 cell line cells. Results： hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes ofhTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRFI-13, ψhTRFI-18, ψhTRF1-21 and ψhTRFI-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells （0.0338, 0.0108-0.0749） than in normal mononuclear cells （0.0493, 0.0369-0.128） （P=0.004）. But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part ofintron 1, 2 and 8 ofhTRF1. Their infection on gene function is unknown and needs further studies. Conclusion： hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.
基金Supported by the Science and Technology Research Project of Chongqing Municipal Education Commission(KJQN201901505)the Key Project of Humanities and Social Sciences Research of Chongqing Education Commission in 2019(19SKGH181)
文摘In this paper,we investigate the multiple attribute decision making(MADM)problems in which the attribute values take the form of hesitant trapezoid fuzzy information.Firstly,inspired by the idea of hesitant fuzzy sets and trapezoid fuzzy numbers,the definition of hesitant trapezoid fuzzy set and some operational laws of hesitant trapezoid fuzzy elements are proposed.Then some hesitant trapezoid fuzzy aggregation operators based on Hamacher operation are developed,such as the hesitant trapezoid fuzzy Hamacher weighted average(HTr FHWA)operator,the hesitant trapezoid fuzzy Hamacher weighted geometric(HTr FHWG)operator,the hesitant trapezoid fuzzy Hamacher Choquet average(HTr FHCA),the hesitant trapezoid fuzzy Hamacher Choquet geometric(HTr FHCG),etc.Furthermore,an approach based on the hesitant trapezoid fuzzy Hamacher weighted average(HTr FHWA)operator and the hesitant trapezoid fuzzy Hamacher weighted geometric(HTr FHWG)operator is proposed for MADM problems under hesitant trapezoid fuzzy environment.Finally,a numerical example for supplier selection is given to illustrate the application of the proposed approach.
