Bcterial strain HTG7 is isolated from extremely glyphosate-polluted soil. It is identified as Halomonas Varabilis. It can tolerate in 500 m mol/L glyphosate concentration. Physiological characterization of strain HTG7...Bcterial strain HTG7 is isolated from extremely glyphosate-polluted soil. It is identified as Halomonas Varabilis. It can tolerate in 500 m mol/L glyphosate concentration. Physiological characterization of strain HTG7 shows that the optimum pH and temperature are 7.0 and 30℃, respectively. It grows well in the NaCl concentrations ranging from 0% to 10%. A plasmid pACYC184 carrying a 3.5 kb DNA fragment, which confers increased glyphosate tolerance, is cloned. The DNA fragment is able to complement with an E.coli auxotrophic aroA mutant.展开更多
基金国家高技术研究发展计划(863计划)，Key Technologies Research and Development Program on Special Problems and Specific Project for Gene Transferring
文摘Bcterial strain HTG7 is isolated from extremely glyphosate-polluted soil. It is identified as Halomonas Varabilis. It can tolerate in 500 m mol/L glyphosate concentration. Physiological characterization of strain HTG7 shows that the optimum pH and temperature are 7.0 and 30℃, respectively. It grows well in the NaCl concentrations ranging from 0% to 10%. A plasmid pACYC184 carrying a 3.5 kb DNA fragment, which confers increased glyphosate tolerance, is cloned. The DNA fragment is able to complement with an E.coli auxotrophic aroA mutant.
