The bench top test is one of the most important and effective methods to evaluate the total thermal protective performance(TPP) of firefighters' protective clothing,which is greatly influenced by the air gaps entr...The bench top test is one of the most important and effective methods to evaluate the total thermal protective performance(TPP) of firefighters' protective clothing,which is greatly influenced by the air gaps entrapped.In this paper,to investigate the effect of air gap width on TPP,a new improved apparatus with two height changeable buttons to hold the thermal sensor was developed to get a series of air gap sizes from 0 mm to 40 mm.The TPP of two types of flame-resistant outer fabrics was measured with TPP test apparatus respectively.Analysis of temperature rise with each air gap width was made to determine the effects of different air gaps on protective performance.It was indicated that air gap size had great effect on TPP of fabrics in the bench top test.An air gap width above 8 mm was suggested for the thermal protective clothing design.展开更多
Telomerase is a unique ribonucleoprotein complex that protects chromosomes from degradation and recombination. The defects in telomerase activity eansed by the mutations in telomerase RNA （hTER） is associated with s...Telomerase is a unique ribonucleoprotein complex that protects chromosomes from degradation and recombination. The defects in telomerase activity eansed by the mutations in telomerase RNA （hTER） is associated with several human genetic diseases, so the molecular biology and structural biology of hTER are of great significance for the treatment of related diseases and the development of corresponding drugs. In this study, hTER was obtained by in vitro transcription, and then modified with N-methylisatoic anhydride （NMIA） by SHAPE （selective 2＇-hydroxyl aeylation analyzed by primer extension） ; finally, the eDNA fragment was obtained by reverse transcription to analyze the free nucleotides and dynamic structure of hTER, which may provide experimental basis for analyzing three--di- mensional structure of hTER obtained in vitro. In addition, the SHAPE parameters for long RNA fragment （ 〉 300 nt） were optimized. The results showed that 1 μmol/L hTER and 3.9 mmol/L NMIA were optimal, and 49 ℃ was the best temperature for primer extension.展开更多
基金National Natural Science Foundation of China (No. 50876019)the Research Fund for the Doctoral Program of Higher Education of China (No. 200802550009)Fundamental Research Funds for the Central Universities,China
文摘The bench top test is one of the most important and effective methods to evaluate the total thermal protective performance(TPP) of firefighters' protective clothing,which is greatly influenced by the air gaps entrapped.In this paper,to investigate the effect of air gap width on TPP,a new improved apparatus with two height changeable buttons to hold the thermal sensor was developed to get a series of air gap sizes from 0 mm to 40 mm.The TPP of two types of flame-resistant outer fabrics was measured with TPP test apparatus respectively.Analysis of temperature rise with each air gap width was made to determine the effects of different air gaps on protective performance.It was indicated that air gap size had great effect on TPP of fabrics in the bench top test.An air gap width above 8 mm was suggested for the thermal protective clothing design.
文摘Telomerase is a unique ribonucleoprotein complex that protects chromosomes from degradation and recombination. The defects in telomerase activity eansed by the mutations in telomerase RNA （hTER） is associated with several human genetic diseases, so the molecular biology and structural biology of hTER are of great significance for the treatment of related diseases and the development of corresponding drugs. In this study, hTER was obtained by in vitro transcription, and then modified with N-methylisatoic anhydride （NMIA） by SHAPE （selective 2＇-hydroxyl aeylation analyzed by primer extension） ; finally, the eDNA fragment was obtained by reverse transcription to analyze the free nucleotides and dynamic structure of hTER, which may provide experimental basis for analyzing three--di- mensional structure of hTER obtained in vitro. In addition, the SHAPE parameters for long RNA fragment （ 〉 300 nt） were optimized. The results showed that 1 μmol/L hTER and 3.9 mmol/L NMIA were optimal, and 49 ℃ was the best temperature for primer extension.
