Objective： To clone the full-length cDNA sequence of HSPC016 gene, an aggregative growth related gene in dermal papilla cells （DPC）, and analyze its characteristics and predict its biological function. Methods： Ra...Objective： To clone the full-length cDNA sequence of HSPC016 gene, an aggregative growth related gene in dermal papilla cells （DPC）, and analyze its characteristics and predict its biological function. Methods： Rapid amplification of cDNA ends （RACE） technology was entailed to amplify the 5＇ and 3＇ sequences of HSPC016. The amplified fragments were TA-cloned, sequenced and spliced together to obtain the full-length cDNA. Its chromosome localization, domain and possible function were analyzed by bioinformatic methods. Results.. Two isoforms, 400 bp and 493 bp, were obtained. The gene was mapped on chromosome 3q21. 31, and was conservative on evolution. HSPC016, a 64aa protein, belongs to PD053992 protein family and its functional domain was homologous to T2FA gene. Conclusion： PISPC016 may be related to transcriptional regulation and its protein product may act as a subunit of a transcriptional complex and play a role on DPC growth and differentiation through facilitating or suppressing other genes＇ transcription within the nucleus.展开更多
基金Supported by the National Natural Science Foundation of China (No.30200249)
文摘Objective： To clone the full-length cDNA sequence of HSPC016 gene, an aggregative growth related gene in dermal papilla cells （DPC）, and analyze its characteristics and predict its biological function. Methods： Rapid amplification of cDNA ends （RACE） technology was entailed to amplify the 5＇ and 3＇ sequences of HSPC016. The amplified fragments were TA-cloned, sequenced and spliced together to obtain the full-length cDNA. Its chromosome localization, domain and possible function were analyzed by bioinformatic methods. Results.. Two isoforms, 400 bp and 493 bp, were obtained. The gene was mapped on chromosome 3q21. 31, and was conservative on evolution. HSPC016, a 64aa protein, belongs to PD053992 protein family and its functional domain was homologous to T2FA gene. Conclusion： PISPC016 may be related to transcriptional regulation and its protein product may act as a subunit of a transcriptional complex and play a role on DPC growth and differentiation through facilitating or suppressing other genes＇ transcription within the nucleus.
