A human semicircular canal(HSC)with“cupulolithiasis”(HSCC)causes abnormal perception and vertigo.Based on 3D printing technology and target tracking technology,models of a visualized bionic semicircular canal with c...A human semicircular canal(HSC)with“cupulolithiasis”(HSCC)causes abnormal perception and vertigo.Based on 3D printing technology and target tracking technology,models of a visualized bionic semicircular canal with cupulolithiasis(BSCC)were generated.The model,with careful scaling parameters,similar biomechanical responses to the vestibular-ocular reflex(VOR),and a similarly long time constant to the HSC,allows us to study the mechanics of the HSCC.The static experiments revealed that the bionic cupula of the BSCC continued to shift due to the effect of the gravity of the otolith after rotation stopped.The frequency broadband experiment indicated that the gain of the BSCC decreased as the phase difference increased,and the increase in otolith mass aggravated this trend.BSCCs can be used as a bionic model to study the pathology of human semicircular canal-related diseases and may promote the development of treatments.展开更多
Objective: To screen out a set of candidate genes which could help to determine whether patients with hypopharyngeal squamous cell carcinoma (HSCC) could benefit from docetaxel, cisplatin and 5-fluorouracil (TPF) indu...Objective: To screen out a set of candidate genes which could help to determine whether patients with hypopharyngeal squamous cell carcinoma (HSCC) could benefit from docetaxel, cisplatin and 5-fluorouracil (TPF) induction chemotherapy. Methods: Gene-expression profiles in 12 TPF-sensitive patients were compared to 9 resistant controls by microarray analysis. Subsequently, expression levels of potential biomarkers in chemosensitive cell line FaDu after TPF treatment were observed by quantitative real-time polymerase chain reaction (qRT-PCR). Results: Through microarray analysis, 1,579 differentially expressed genes were identified, of which 815 were up-regulated in TPF chemotherapy-responsive tissues whereas 764 were down-regulated. Gene ontology (GO) analysis suggested these genes participating in physiological processes including transcription and its regulation, cellular signal transduction and metabolic process. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) database revealed that MAPK and Jat/STAT signaling pathways occupied important roles in TPF chemotherapeutic sensitivity. Moreover, in vitro cell culture experiments revealed the expression alternations of IL-6, MAPK14, JUN, CDK5 and CAMK2A exposed to TPF treatment by qRT-PCR, whilst providing an insight into the mechanism underlying TPF chemotherapeutic response in HSCC. Conclusions: These results provided a battery of genes related to TPF chemotherapeutic sensitivity and might act as molecular targets in HSCC treatment. Moreover, these candidate biomarkers could contribute to HSCC individualized treatment.展开更多
基金supported by the National Key Research and Development Program of China(No.2024YFD2000203)the National Natural Science Foundation of China(No.51775483).
文摘A human semicircular canal(HSC)with“cupulolithiasis”(HSCC)causes abnormal perception and vertigo.Based on 3D printing technology and target tracking technology,models of a visualized bionic semicircular canal with cupulolithiasis(BSCC)were generated.The model,with careful scaling parameters,similar biomechanical responses to the vestibular-ocular reflex(VOR),and a similarly long time constant to the HSC,allows us to study the mechanics of the HSCC.The static experiments revealed that the bionic cupula of the BSCC continued to shift due to the effect of the gravity of the otolith after rotation stopped.The frequency broadband experiment indicated that the gain of the BSCC decreased as the phase difference increased,and the increase in otolith mass aggravated this trend.BSCCs can be used as a bionic model to study the pathology of human semicircular canal-related diseases and may promote the development of treatments.
基金supported by grants of the Beijing Municipal Science&Technology Commission(No.Z141107002514003)Beijing Municipal Administration of Hospital Clinical Medicine Development of Special Funding Support(Code.XMLX201311)the Priming Scientific Research Foundation for the Junior Researcher in Beijing Tongren Hospital,Capital Medical University(2015-YJJ-ZZL-001)
文摘Objective: To screen out a set of candidate genes which could help to determine whether patients with hypopharyngeal squamous cell carcinoma (HSCC) could benefit from docetaxel, cisplatin and 5-fluorouracil (TPF) induction chemotherapy. Methods: Gene-expression profiles in 12 TPF-sensitive patients were compared to 9 resistant controls by microarray analysis. Subsequently, expression levels of potential biomarkers in chemosensitive cell line FaDu after TPF treatment were observed by quantitative real-time polymerase chain reaction (qRT-PCR). Results: Through microarray analysis, 1,579 differentially expressed genes were identified, of which 815 were up-regulated in TPF chemotherapy-responsive tissues whereas 764 were down-regulated. Gene ontology (GO) analysis suggested these genes participating in physiological processes including transcription and its regulation, cellular signal transduction and metabolic process. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) database revealed that MAPK and Jat/STAT signaling pathways occupied important roles in TPF chemotherapeutic sensitivity. Moreover, in vitro cell culture experiments revealed the expression alternations of IL-6, MAPK14, JUN, CDK5 and CAMK2A exposed to TPF treatment by qRT-PCR, whilst providing an insight into the mechanism underlying TPF chemotherapeutic response in HSCC. Conclusions: These results provided a battery of genes related to TPF chemotherapeutic sensitivity and might act as molecular targets in HSCC treatment. Moreover, these candidate biomarkers could contribute to HSCC individualized treatment.
