A rapid pretreatment method of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in soil and analysis by high resolution gas chromatograph-high resolution mass spectrometry was present.The extraction and a...A rapid pretreatment method of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in soil and analysis by high resolution gas chromatograph-high resolution mass spectrometry was present.The extraction and alumina clean up of PCDD/Fs in soil was achieved by accelerated solvent extractor.Then the multi-layer silica-gel column was used for further clean up.The whole method has been evaluated on certified reference soil and farm soil.Accuracy and precision of this method was tested with satisfactory results.展开更多
Polychlorinated biphenyls(PCBs) are stable in the environment,undergo long-range atmospheric transport,and possess the ability to bioaccumulate through the food chain which can impose a serious threat to human health ...Polychlorinated biphenyls(PCBs) are stable in the environment,undergo long-range atmospheric transport,and possess the ability to bioaccumulate through the food chain which can impose a serious threat to human health and the environment.Atmosphere is the major pathway for PCBs to transport and redistribute in the environment.In order to know levels and patterns of PCBs in background air in China,high-volume air samples were collected at 11 background sites from November 2007 to February 2008.PCBs were extracted from samples by accelerated solvent extraction(ASE) and then purified by acid silica column and fluid management systems(FMS).Confirmation and quantitative analysis were performed by HRGC-HRMS using voltage selective ion record mode.Total concentrations of dioxin-like PCBs in background air ranged from 348.8 fg/m3 to 1 768.3 fg/m3(0.7-7.4 fg TEQ/m3),and total concentrations of indicator PCBs were from 9.0 pg/m3 up to 85.2 pg/m3,which were both lower than the concentrations of most other regions and countries.展开更多
The status of PCDDs and PCDFs content in retail foods from a certain area by Isotope Dilution HRGC-HRMS was surveyed and the local population PCDD/Fs exposure from diverse foods and health risk was evaluated.PCDD/Fs w...The status of PCDDs and PCDFs content in retail foods from a certain area by Isotope Dilution HRGC-HRMS was surveyed and the local population PCDD/Fs exposure from diverse foods and health risk was evaluated.PCDD/Fs was extracted from samples by Soxhlet extraction,concentrated and purified by FMS column chromatograph,carbon column enrichment.Confirmation and quantitative analysis at pg/g level of PCDD/Fs was performed by HRGC/HRMS using multiple ion detection mode(MID).TEQ concentration was calculated by WHO-TEF multiplying by concentration of seventeen PCDD/Fs congener.Median of PCDD/Fs concentration for fish,livestock,poultry,egg,vegetable oil,milk,vegetable totally 100 samples for ten diverse foods didn’t exceed the limit standards by EU.The level of PCDD/Fs for different food in the certain area was lower or comparable to the data reported by developed country in the world.The total weekly intake and monthly intake for local population or national population was 3.44,14.8 WHO-TEQ/kg BW and 1.5,6.42 pg WHO-TEQ/kg BW respectively,the value was lower than the Tolerable Weekly Intake of 14 pg WHO TEQ/kg BW for PCDDs,PCDFs and dioxin-like PCBs established by EU Scientific Committee for Food.The dietary PCDD/Fs intake for local people was higher than national population.And animal food was the dominant contributor to the total dietary intake,which accounted for more than 70 percent.These levels of consumption of diverse food containing typical levels of PCDD/Fs doesn’t present a risk to the health of the local population.But integrative dietary intake could be evaluated including of PCBs intake for population in the future.展开更多
酶联免疫法(enzyme immunoassay method,EIA)作为一种二口恶英物质的半定量筛选方法被越来越多的实验室使用。与经典的高分辩气相色谱-高分辨质谱法(high-resolution gas chromatrography coupled with high-resolution mass spectromet...酶联免疫法(enzyme immunoassay method,EIA)作为一种二口恶英物质的半定量筛选方法被越来越多的实验室使用。与经典的高分辩气相色谱-高分辨质谱法(high-resolution gas chromatrography coupled with high-resolution mass spectrometry,HRGC-HRMS)不同,ELISA方法得到检测样本总二口恶英类物质的含量,两种方法之间存在偏差。文章引入校正调节因子(calibration adjustment factor,CAF)来减少这种偏差。通过对本实验室前期工作中得到的47组土壤样品二口恶英数据进行统计分析,计算得到土壤来源二口恶英EIA检测方法的校正调节因子为0.6。后期实验室进行崇明岛土壤二口恶英水平研究时,对得到的土壤样品EIA检测结果使用本文得到的CAF值进行校正,验证CAF值的作用。校正前,两组数据之间存在显著性差异,校正后,数据相近,且无显著性差异。证明此CAF值在土壤样品中的适用性。展开更多
文摘A rapid pretreatment method of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in soil and analysis by high resolution gas chromatograph-high resolution mass spectrometry was present.The extraction and alumina clean up of PCDD/Fs in soil was achieved by accelerated solvent extractor.Then the multi-layer silica-gel column was used for further clean up.The whole method has been evaluated on certified reference soil and farm soil.Accuracy and precision of this method was tested with satisfactory results.
文摘Polychlorinated biphenyls(PCBs) are stable in the environment,undergo long-range atmospheric transport,and possess the ability to bioaccumulate through the food chain which can impose a serious threat to human health and the environment.Atmosphere is the major pathway for PCBs to transport and redistribute in the environment.In order to know levels and patterns of PCBs in background air in China,high-volume air samples were collected at 11 background sites from November 2007 to February 2008.PCBs were extracted from samples by accelerated solvent extraction(ASE) and then purified by acid silica column and fluid management systems(FMS).Confirmation and quantitative analysis were performed by HRGC-HRMS using voltage selective ion record mode.Total concentrations of dioxin-like PCBs in background air ranged from 348.8 fg/m3 to 1 768.3 fg/m3(0.7-7.4 fg TEQ/m3),and total concentrations of indicator PCBs were from 9.0 pg/m3 up to 85.2 pg/m3,which were both lower than the concentrations of most other regions and countries.
文摘The status of PCDDs and PCDFs content in retail foods from a certain area by Isotope Dilution HRGC-HRMS was surveyed and the local population PCDD/Fs exposure from diverse foods and health risk was evaluated.PCDD/Fs was extracted from samples by Soxhlet extraction,concentrated and purified by FMS column chromatograph,carbon column enrichment.Confirmation and quantitative analysis at pg/g level of PCDD/Fs was performed by HRGC/HRMS using multiple ion detection mode(MID).TEQ concentration was calculated by WHO-TEF multiplying by concentration of seventeen PCDD/Fs congener.Median of PCDD/Fs concentration for fish,livestock,poultry,egg,vegetable oil,milk,vegetable totally 100 samples for ten diverse foods didn’t exceed the limit standards by EU.The level of PCDD/Fs for different food in the certain area was lower or comparable to the data reported by developed country in the world.The total weekly intake and monthly intake for local population or national population was 3.44,14.8 WHO-TEQ/kg BW and 1.5,6.42 pg WHO-TEQ/kg BW respectively,the value was lower than the Tolerable Weekly Intake of 14 pg WHO TEQ/kg BW for PCDDs,PCDFs and dioxin-like PCBs established by EU Scientific Committee for Food.The dietary PCDD/Fs intake for local people was higher than national population.And animal food was the dominant contributor to the total dietary intake,which accounted for more than 70 percent.These levels of consumption of diverse food containing typical levels of PCDD/Fs doesn’t present a risk to the health of the local population.But integrative dietary intake could be evaluated including of PCBs intake for population in the future.
文摘酶联免疫法(enzyme immunoassay method,EIA)作为一种二口恶英物质的半定量筛选方法被越来越多的实验室使用。与经典的高分辩气相色谱-高分辨质谱法(high-resolution gas chromatrography coupled with high-resolution mass spectrometry,HRGC-HRMS)不同,ELISA方法得到检测样本总二口恶英类物质的含量,两种方法之间存在偏差。文章引入校正调节因子(calibration adjustment factor,CAF)来减少这种偏差。通过对本实验室前期工作中得到的47组土壤样品二口恶英数据进行统计分析,计算得到土壤来源二口恶英EIA检测方法的校正调节因子为0.6。后期实验室进行崇明岛土壤二口恶英水平研究时,对得到的土壤样品EIA检测结果使用本文得到的CAF值进行校正,验证CAF值的作用。校正前,两组数据之间存在显著性差异,校正后,数据相近,且无显著性差异。证明此CAF值在土壤样品中的适用性。
