目的:构建人宫颈癌HPC2基因的原核及真核表达载体,进一步研究该基因在宫颈癌发生中的作用。方法:从人宫颈癌HeLa细胞中提取总RNA。用RT-PCR方法扩增出人宫颈癌HPC2全序列基因,插入pT7 b lue载体。酶切鉴定及序列分析后,构建人HPC2基因...目的:构建人宫颈癌HPC2基因的原核及真核表达载体,进一步研究该基因在宫颈癌发生中的作用。方法:从人宫颈癌HeLa细胞中提取总RNA。用RT-PCR方法扩增出人宫颈癌HPC2全序列基因,插入pT7 b lue载体。酶切鉴定及序列分析后,构建人HPC2基因的原核及真核表达载体。结果:成功扩增出人宫颈癌HPC2基因;并构建pGEX4T1-HPC2原核表达载体及pCMV-F lag-HPC2真核表达载体。结论:人HPC2基因的原核及真核表达载体的构建为深入研究HPC2基因与宫颈癌的发生奠定了基础。展开更多
The imperative quest for renewable energy sources and advanced energy storage technologies has arisen amidst the escalating perils of climate change and dwindling fossil fuel reserves.In the realm of energy storage te...The imperative quest for renewable energy sources and advanced energy storage technologies has arisen amidst the escalating perils of climate change and dwindling fossil fuel reserves.In the realm of energy storage technologies,asymmetric supercapacitor(ASC)has garnered significant attention owing to its high energy density and power density.In the quest for advanced electrode materials for ASC,the integration of 2D layered heterostructures on hierarchical porous carbon(HPC)substrates has emerged as a promising approach to enhance the electrochemical performance.Herein,a highly innovative hierarchical NiCo LDH/MoS_(2)/HPC heterostructure was successfully synthesized using a simple two-step hydrothermal method for the electrode materials of ASC.Benefiting from the unique hierarchical heterostructure of NiCo LDH/MoS_(2)/HPC composite and the synergistic effect between the components,it reveals an exceptional specific capacitance of 2368 F/g at 0.5 A/g in a three-electrode system,which significantly exceeds that of conventional supercapacitor electrodes.Additionally,the ASC device of NiCo LDH/MoS_(2)/HPC//HPC achieves remarkable specific capacitance of 236 F/g at 0.5 A/g and an impressive energy density of 84Wh/kg at a power density of 400 W/kg,as well as superior cyclic stability.This study not only demonstrates the effectiveness of incorporating MoS_(2) and NiCo LDH into a carbon-based framework for supercapacitor applications but also opens avenues for designing more efficient energy storage devices.展开更多
文摘目的:构建人宫颈癌HPC2基因的原核及真核表达载体,进一步研究该基因在宫颈癌发生中的作用。方法:从人宫颈癌HeLa细胞中提取总RNA。用RT-PCR方法扩增出人宫颈癌HPC2全序列基因,插入pT7 b lue载体。酶切鉴定及序列分析后,构建人HPC2基因的原核及真核表达载体。结果:成功扩增出人宫颈癌HPC2基因;并构建pGEX4T1-HPC2原核表达载体及pCMV-F lag-HPC2真核表达载体。结论:人HPC2基因的原核及真核表达载体的构建为深入研究HPC2基因与宫颈癌的发生奠定了基础。
基金supported by the National Key Research and Development Program of China(No.2021YFB3801200)the National Natural Science Foundation of China(Nos.22278051,22178044,and 22308043)the Science and Technology Innovation foundation of CNPC(No.2022DQ02–0608).
文摘The imperative quest for renewable energy sources and advanced energy storage technologies has arisen amidst the escalating perils of climate change and dwindling fossil fuel reserves.In the realm of energy storage technologies,asymmetric supercapacitor(ASC)has garnered significant attention owing to its high energy density and power density.In the quest for advanced electrode materials for ASC,the integration of 2D layered heterostructures on hierarchical porous carbon(HPC)substrates has emerged as a promising approach to enhance the electrochemical performance.Herein,a highly innovative hierarchical NiCo LDH/MoS_(2)/HPC heterostructure was successfully synthesized using a simple two-step hydrothermal method for the electrode materials of ASC.Benefiting from the unique hierarchical heterostructure of NiCo LDH/MoS_(2)/HPC composite and the synergistic effect between the components,it reveals an exceptional specific capacitance of 2368 F/g at 0.5 A/g in a three-electrode system,which significantly exceeds that of conventional supercapacitor electrodes.Additionally,the ASC device of NiCo LDH/MoS_(2)/HPC//HPC achieves remarkable specific capacitance of 236 F/g at 0.5 A/g and an impressive energy density of 84Wh/kg at a power density of 400 W/kg,as well as superior cyclic stability.This study not only demonstrates the effectiveness of incorporating MoS_(2) and NiCo LDH into a carbon-based framework for supercapacitor applications but also opens avenues for designing more efficient energy storage devices.
