In the Chinese lunar calendar,2026 ushers in the Year of the Horse,a creature that has carried humanity across continents and through centuries.More than a marker of time,the horse embodies vitality,courage,and the re...In the Chinese lunar calendar,2026 ushers in the Year of the Horse,a creature that has carried humanity across continents and through centuries.More than a marker of time,the horse embodies vitality,courage,and the relentless spirit to move forward,qualities that resonate not only in China but across the landscapes of many African societies.展开更多
In Chinese culture,the horse is a symbol of vigor,strength,and resilience,heralding a future of steady progress and prosperous development,President Xi Jinping said when extending Spring Festival greetings at a recept...In Chinese culture,the horse is a symbol of vigor,strength,and resilience,heralding a future of steady progress and prosperous development,President Xi Jinping said when extending Spring Festival greetings at a reception on February 14 in Beijing to ring in the Chinese New Year-the Year of the Horse.This statement,resonating with the heart of the world’s most populous national celebration,offers a profound lens through which to understand not just a festival,but the pulsating energy of contemporary China.展开更多
Located in Hangzhou,Zhejiang Province,the Sijiqing Clothing Street is one of the largest clothing distribution centers in China.It gathers 23 professional markets and over 15,000 businesses.In November 2025,merchants ...Located in Hangzhou,Zhejiang Province,the Sijiqing Clothing Street is one of the largest clothing distribution centers in China.It gathers 23 professional markets and over 15,000 businesses.In November 2025,merchants were already preparing for sales during the 2026 Spring Festival and the spring/summer seasons.Children's clothing is one of the important product categories.What are the new trends in children's clothing sales?And what new changes are happening in this traditional wholesale market?展开更多
Chinese New Year is a time of vibrant energy and delightful surprises.Streets across China glow with red and gold decorations,while the air carries the scent of freshly steamed pastries and a wide variety of sweet tre...Chinese New Year is a time of vibrant energy and delightful surprises.Streets across China glow with red and gold decorations,while the air carries the scent of freshly steamed pastries and a wide variety of sweet treats.As the Year of the Horse gallops in,it brings a surge of vitality to the communities.Beyond fireworks,decorations,and dragon dances.展开更多
In autumn on the Changtang Grassland,the morning light turns golden,and the dew has not yet evaporated.On the Gesar Horse Racing Field in the Seni District of Nagqu,at an altitude of 4,500 meters,the crowd surged and ...In autumn on the Changtang Grassland,the morning light turns golden,and the dew has not yet evaporated.On the Gesar Horse Racing Field in the Seni District of Nagqu,at an altitude of 4,500 meters,the crowd surged and the cheers rise like a tidal wave,marking the start of the annual Nagqu Qiaqing Horse Racing Festival.Herdsmen from 11 districts and counties ride their horses,dress in magnificent costumes,their sleeves fluttering in the wind and their silver ornaments chiming.Their faces are etched with traces of wind and sunlight,and their spirits exude the pride of a culture passed down through the generations.展开更多
Chakouyi(CKY)horses from the Qinghai-Xizang Plateau are well known for their unique lateral gaits and high-altitude adaptation,but genetic mechanisms underlying these phenotypes remain unclear.This study presents a co...Chakouyi(CKY)horses from the Qinghai-Xizang Plateau are well known for their unique lateral gaits and high-altitude adaptation,but genetic mechanisms underlying these phenotypes remain unclear.This study presents a comparison of 60 newly resequenced genomes of gaited CKY horses with 139 public genomes from 19 horse breeds.Population structure analyses(admixture,PCA,and neighbor-joining tree)reveal a close genetic relationship between CKY and other highland breeds(Tibetan and Chaidamu horses).Compared with other Chinese breeds,CKY horses present reduced nucleotide diversity(θπ)and lower inbreeding(FROHcoefficient),suggesting possible selective pressures.A key region on chromosome 23(Chr23:22.3-22.6 Mb)is associated with the lateral gaits and harbors a highly prevalent nonsense mutation(Chr 23:22,391,254 C>A,Ser301STOP)in the DMRT3 gene,with an 88%homozygosity rate,which is strongly correlated with the distinctive gait of CKY horses.Furthermore,selection signals reveal that the EPAS1 gene is related to high-altitude adaptation,and the CAT gene contributes to altitude resilience in CKY horses.These findings suggest that preserving genetic diversity is essential for maintaining the unique gaits and high-altitude adaptations of CKY horses.展开更多
Along the ancient Tea-Horse Road of thousands of miles between Sichuan and Xizang in Southwest China lies a centuries old courtyard.Commonly called the"Jiang Family Compound"or"Yu Xing Tea Store",t...Along the ancient Tea-Horse Road of thousands of miles between Sichuan and Xizang in Southwest China lies a centuries old courtyard.Commonly called the"Jiang Family Compound"or"Yu Xing Tea Store",this architecture situated in the old downtown area of Yingjing County in Ya'an City,Sichuan Province,was once the residence of the local tea merchants-the Jiang family.展开更多
Gastrointestinal(GI)parasites are a global concern and cause serious damage to the health of animals,including horses,because of their deleterious effects on the health of these animals.Owing to improperly diagnosing ...Gastrointestinal(GI)parasites are a global concern and cause serious damage to the health of animals,including horses,because of their deleterious effects on the health of these animals.Owing to improperly diagnosing the parasitic load and the resulting incorrect treatment,anthelmintics are becoming a global problem.Although rapid diagnostic techniques such as McMaster and FECPAK^(G2) are available in Africa,concentration techniques,including flotation and sedimentation,are commonly used in screening for gastrointestinal parasites.In this study,we compared the sensitivity of diagnostic techniques,namely,McMaster and FECPAK^(G2),in quantifying and determining the prevalence of gastrointestinal parasites as an alternative to the commonly used flotation and sedimentation techniques for equine fecal samples in Jos,Plateau State,Nigeria.Three hundred fecal samples were collected from different locations.The instructions provided by the manufacturers of FECPAK^(G2) and McMasters were used as the methodology for egg count per gram of fecal matter,while parasite egg/ova identification was performed through sedimentation and flotation techniques.In general,a prevalence of 66.3%was recorded,with a higher prevalence on Babale ranch(84%)than on Jos Polo Club(62%)and Jos mounted troop(53%).A total of nine gastrointestinal parasites were identified via both sedimentation and flotation techniques:Strongylus spp.(19.3%)was the most prevalent parasite identified via the flotation method,whereas Gastrodiscus aegyptiacus(25.3%)was the most prevalent parasite identified via the sedimentation technique.In general,FECPAK^(G2) had a sensitivity of 86%,whereas McMaster had a sensitivity of 64%in terms of egg counts per gram of fecal sample.This finding suggests that equines in Jos are highly infected with a variety of gastrointestinal parasites to varying degrees,with Strongylus spp.and Gastrodiscus aegyptiacus being more prevalent.Although the parasite load was found to range from low to moderate levels,the majority of the equine samples contained<200 eggs per gram,suggesting a low infection rate via both the McMaster technique and the FECPAK^(G2) technique.On the basis of these findings,FECPAK^(G2) is recommended for rapid diagnosis because of its prompt outcome and infestation rate as well as ease of routine examination.Additionally,routine examination should be carried out to determine infestation by gastrointestinal parasites in all equine holdings and the effectiveness of the therapeutic agents used.展开更多
Once there lived a very old peasant in a village. His name was Jack.He had a horse.It was almost as old as him. One day he set off with his young son to sell the horse before it died.They walked because the father did...Once there lived a very old peasant in a village. His name was Jack.He had a horse.It was almost as old as him. One day he set off with his young son to sell the horse before it died.They walked because the father did not want the horse to be too tired. They met two neighbours (邻居)on their way.The neighbours said, "Hello,why are展开更多
In this paper numerical analysis of underground structures, taking account the transverse isotropy system of rocks, was done using CAST 3M code by varying the shape of excavation and the coefficient of earth pressure ...In this paper numerical analysis of underground structures, taking account the transverse isotropy system of rocks, was done using CAST 3M code by varying the shape of excavation and the coefficient of earth pressure k. Numerical results reveal that the anisotropy behavior, the shape of hole and the coefficient of earth pressure k have significant influence to the mining induced stress field and rock deformations which directly control the stability of underground excavation design. The magnitude of horizontal stress obtained for the horse shoe shape excavation(25.2 MPa for k = 1; 52.7 MPa for k = 2)is lower than the magnitude obtained for circular hole(26.4 MPa for k = 1; 59.5 MPa for k = 2).Therefore, we have concluded that the horse shoe shape offers the best stability and the best design for engineer. The anisotropy system presented by rock mass can also influence the redistribution of stresses around hole opened. Numerical results have revealed that the magnitude of redistribution of horizontal stresses obtained for transverse isotropic rock(12.1 MPa for k = 0.5; 25.2 MPa for k = 1 and52.7 MPa for k = 2) is less than those obtained in the case of isotropic rock(27.6 MPa for k = 1;48.6 MPa for k = 2 and 90.81 MPa for k = 2). The more the rock has the anisotropic behavior, the more the mass of rock around the tunnel is stable.展开更多
AIM： To evaluate the efficacy and safety of orbital decomposition （OD） surgery in combination with horse chestnut seed extract （HCSE）, as compared to OD atone, in patients with thyroid-associated ophthalmopathy ...AIM： To evaluate the efficacy and safety of orbital decomposition （OD） surgery in combination with horse chestnut seed extract （HCSE）, as compared to OD atone, in patients with thyroid-associated ophthalmopathy （TAO）. METHODS： Sixty-two orbits from 62 TAO patients were randomly assigned to OD or OD＋HCSE at 1：1 ratio （31 received OD alone, 31 received OD ＋HCSE）. Forty-two orbits from 21 healthy subjects were used as controls. Complete ophthalmic examination and color Doppler flow imaging （CDFI） were performed before surgery and 3mo post-surgery on all 62 orbits from the TAO patients. CDFI were also performed on the 42 control orbits, The effect of OD ＋HCSE and OD alone on TAO orbits was compared on several endpoints, including superior ophthalmic vein blood flow （SOVBF） parameters, subjective assessment, soft tissue involvement, lid retraction, diplopia, eye movement restriction, degree of exophthalmos, and intraocular pressure. The control orbits were used as reference for the SOVBF parameters. RESULTS： OD surgery with or without HCSE improved SOVBF, symptoms and soft tissue involvement, decreased degree of exophthalmos and intraocular pressure in orbits of TAO patients. The OD ＋HCSE combination led to significantly better improvement of SOVBF than OD alone. The differences between the reductions of SOVBF in the two groups are 1.26 cmls in max-volecity and 0.52 cm/s in min-voiecity （P〈0.0001）. CONCLUSION： SOVBF is significantly reduced in the orbits affected with TAO, indicating that congestion may be an important factor contributing to TAO pathogenesis. OD surgery improves the SOVBF, and combination of HCSE medication and OD surgery further improved venous return than OD surgery alone.展开更多
Early-stage brain metastasis of breast cancer(BMBC), due to the existence of an intact blood–brain barrier(BBB), is one of the deadliest neurologic complications. To improve the efficacy of chemotherapy for BMBC, a T...Early-stage brain metastasis of breast cancer(BMBC), due to the existence of an intact blood–brain barrier(BBB), is one of the deadliest neurologic complications. To improve the efficacy of chemotherapy for BMBC, a Trojan horse strategy-based nanocarrier has been developed by integrating the cell membrane of a brain-homing cancer cell and a polymeric drug depot. With the camouflage of a MDA-MB-231/Br cell membrane, doxorubicin-loaded poly(D, L-lactic-co-glycolic acid) nanoparticle(DOX-PLGA@CM) shows enhanced cellular uptake and boosted killing potency for MDA-MB-231/Br cells. Furthermore, DOX-PLGA@CM is equipped with naturally selected molecules for BBB penetration, as evidenced by its boosted capacity in entering the brain of both healthy and early-stage BMBC mouse models. Consequently, DOX-PLGA@CM effectively reaches the metastatic tumor lesions in the brain, slows down cancer progression, reduces tumor burden, and extends the survival time for the BMBC animal.Furthermore, the simplicity and easy scale-up of the design opens a new window for the treatment of BMBC and other brain metastatic cancers.展开更多
A cross sectional study was conducted from October 2010 to June 2011 in and around Gondar town to identify the species and determine prevalence of gastrointestinal parasites in donkeys and horses. Gross examination, d...A cross sectional study was conducted from October 2010 to June 2011 in and around Gondar town to identify the species and determine prevalence of gastrointestinal parasites in donkeys and horses. Gross examination, direct fecal smear, sedimentation and floatation techniques were utilized to identify the eggs and larvae of parasites in feces. A total of 384 horses and donkeys were examined for gastrointestinal parasites. The overall prevalence of gastrointestinal parasites was 92.71% (356 from 384) with 80.95% (85 from 105) and 97.13% (271 from 279) in horses and donkeys, respectively. Prevalence of Strongyle, Parascaris equorum, Oxyuris equi, Gastrodiscus aegyptiacus and Gastrophilus intestinalis was 66.67%, 43.8%, 0.95%, 2.86%, and 0.95%, respectively in horses. Prevalence of gastrointestinal parasites was 87.81%, 42.29%, 4.30%, 5.73%, 1.43%, 3.58% and 0.72% for Strongyles, Parascaris equorum, Oxyuris equi, Fasciola, Tricuris, Gastrodiscus aegyptiacus and Gastrophilus intestinalis in donkeys, respectively. There was a statisticcally significant difference between species, housing and among feed types in prevalence of equine gastrointestinal parasites展开更多
OBJECTIVE: To investigate the efficacy of horse oil on lipopolysaccharide(LPS)-induced inflammation in human keratinocytes.METHODS: Western blot analysis was performed to measure the expression of cyclooxygenase-2(COX...OBJECTIVE: To investigate the efficacy of horse oil on lipopolysaccharide(LPS)-induced inflammation in human keratinocytes.METHODS: Western blot analysis was performed to measure the expression of cyclooxygenase-2(COX-2) and IκBα. ELISA was used to analyze prostaglandin E2(PGE2) levels.RESULTS: Horse oil decreased LPS-induced COX-2 and PGE2 levels in a dose-dependent manner. Nuclear factor-kappa B(NF-κB) plays a key role in the expression of inflammatory cytokines and mediators. Therefore, we investigated the influence of horse oil on the NF-κB signaling pathways. Horse oil inhibited translocation of NF-κB from the cytosol to the nucleus. Furthermore, LPS-induced degradation of IκBα was recovered by horse oil. The activation of p38 mitogen-activated protein kinase(MAPK) reportedly induces degradation of IκBα In agreement with this, LPS activated p38 MAPK and caused IκBα degradation. Conversely, horse oil inhibited LPS-induced p38 MAPK activation and IκBαdegradation. In addition, a specific p38 MAPK inhibitor, SB203580, blocked IκBα degradation.CONCLUSION: Horse oil decreased COX-2 and PGE2 by inhibiting p38 MAPK activation, IκBα degradation, and the translocation of NF-κB.展开更多
African horse sickness(AHS)is an acute and fatal vectorborne infectious disease of equids,caused by the African horse sickness virus(AHSV).The World Organization for Animal Health(WOAH)has classified AHS as a notifiab...African horse sickness(AHS)is an acute and fatal vectorborne infectious disease of equids,caused by the African horse sickness virus(AHSV).The World Organization for Animal Health(WOAH)has classified AHS as a notifiable animal disease,and AHS has also been classified as a Class I animal infectious disease in China.AHS is mainly found in Africa,the Middle East and the Arabian Peninsula.China is currently recognized by the WOAH as an AHS-free zone.展开更多
文摘In the Chinese lunar calendar,2026 ushers in the Year of the Horse,a creature that has carried humanity across continents and through centuries.More than a marker of time,the horse embodies vitality,courage,and the relentless spirit to move forward,qualities that resonate not only in China but across the landscapes of many African societies.
文摘In Chinese culture,the horse is a symbol of vigor,strength,and resilience,heralding a future of steady progress and prosperous development,President Xi Jinping said when extending Spring Festival greetings at a reception on February 14 in Beijing to ring in the Chinese New Year-the Year of the Horse.This statement,resonating with the heart of the world’s most populous national celebration,offers a profound lens through which to understand not just a festival,but the pulsating energy of contemporary China.
文摘Located in Hangzhou,Zhejiang Province,the Sijiqing Clothing Street is one of the largest clothing distribution centers in China.It gathers 23 professional markets and over 15,000 businesses.In November 2025,merchants were already preparing for sales during the 2026 Spring Festival and the spring/summer seasons.Children's clothing is one of the important product categories.What are the new trends in children's clothing sales?And what new changes are happening in this traditional wholesale market?
文摘Chinese New Year is a time of vibrant energy and delightful surprises.Streets across China glow with red and gold decorations,while the air carries the scent of freshly steamed pastries and a wide variety of sweet treats.As the Year of the Horse gallops in,it brings a surge of vitality to the communities.Beyond fireworks,decorations,and dragon dances.
文摘In autumn on the Changtang Grassland,the morning light turns golden,and the dew has not yet evaporated.On the Gesar Horse Racing Field in the Seni District of Nagqu,at an altitude of 4,500 meters,the crowd surged and the cheers rise like a tidal wave,marking the start of the annual Nagqu Qiaqing Horse Racing Festival.Herdsmen from 11 districts and counties ride their horses,dress in magnificent costumes,their sleeves fluttering in the wind and their silver ornaments chiming.Their faces are etched with traces of wind and sunlight,and their spirits exude the pride of a culture passed down through the generations.
基金the members of the Extending Station for Animal Husbandry and Veterinary Technology of Tianzhu Xizang Autonomous County for their help in sample collection and data acquisition.This research was supported by the Supercomputing Center of Lanzhou University and the Chakouyi horse conservation project from the Tianzhu Xizang Autonomous County Government([20]0097).
文摘Chakouyi(CKY)horses from the Qinghai-Xizang Plateau are well known for their unique lateral gaits and high-altitude adaptation,but genetic mechanisms underlying these phenotypes remain unclear.This study presents a comparison of 60 newly resequenced genomes of gaited CKY horses with 139 public genomes from 19 horse breeds.Population structure analyses(admixture,PCA,and neighbor-joining tree)reveal a close genetic relationship between CKY and other highland breeds(Tibetan and Chaidamu horses).Compared with other Chinese breeds,CKY horses present reduced nucleotide diversity(θπ)and lower inbreeding(FROHcoefficient),suggesting possible selective pressures.A key region on chromosome 23(Chr23:22.3-22.6 Mb)is associated with the lateral gaits and harbors a highly prevalent nonsense mutation(Chr 23:22,391,254 C>A,Ser301STOP)in the DMRT3 gene,with an 88%homozygosity rate,which is strongly correlated with the distinctive gait of CKY horses.Furthermore,selection signals reveal that the EPAS1 gene is related to high-altitude adaptation,and the CAT gene contributes to altitude resilience in CKY horses.These findings suggest that preserving genetic diversity is essential for maintaining the unique gaits and high-altitude adaptations of CKY horses.
文摘Along the ancient Tea-Horse Road of thousands of miles between Sichuan and Xizang in Southwest China lies a centuries old courtyard.Commonly called the"Jiang Family Compound"or"Yu Xing Tea Store",this architecture situated in the old downtown area of Yingjing County in Ya'an City,Sichuan Province,was once the residence of the local tea merchants-the Jiang family.
文摘Gastrointestinal(GI)parasites are a global concern and cause serious damage to the health of animals,including horses,because of their deleterious effects on the health of these animals.Owing to improperly diagnosing the parasitic load and the resulting incorrect treatment,anthelmintics are becoming a global problem.Although rapid diagnostic techniques such as McMaster and FECPAK^(G2) are available in Africa,concentration techniques,including flotation and sedimentation,are commonly used in screening for gastrointestinal parasites.In this study,we compared the sensitivity of diagnostic techniques,namely,McMaster and FECPAK^(G2),in quantifying and determining the prevalence of gastrointestinal parasites as an alternative to the commonly used flotation and sedimentation techniques for equine fecal samples in Jos,Plateau State,Nigeria.Three hundred fecal samples were collected from different locations.The instructions provided by the manufacturers of FECPAK^(G2) and McMasters were used as the methodology for egg count per gram of fecal matter,while parasite egg/ova identification was performed through sedimentation and flotation techniques.In general,a prevalence of 66.3%was recorded,with a higher prevalence on Babale ranch(84%)than on Jos Polo Club(62%)and Jos mounted troop(53%).A total of nine gastrointestinal parasites were identified via both sedimentation and flotation techniques:Strongylus spp.(19.3%)was the most prevalent parasite identified via the flotation method,whereas Gastrodiscus aegyptiacus(25.3%)was the most prevalent parasite identified via the sedimentation technique.In general,FECPAK^(G2) had a sensitivity of 86%,whereas McMaster had a sensitivity of 64%in terms of egg counts per gram of fecal sample.This finding suggests that equines in Jos are highly infected with a variety of gastrointestinal parasites to varying degrees,with Strongylus spp.and Gastrodiscus aegyptiacus being more prevalent.Although the parasite load was found to range from low to moderate levels,the majority of the equine samples contained<200 eggs per gram,suggesting a low infection rate via both the McMaster technique and the FECPAK^(G2) technique.On the basis of these findings,FECPAK^(G2) is recommended for rapid diagnosis because of its prompt outcome and infestation rate as well as ease of routine examination.Additionally,routine examination should be carried out to determine infestation by gastrointestinal parasites in all equine holdings and the effectiveness of the therapeutic agents used.
文摘Once there lived a very old peasant in a village. His name was Jack.He had a horse.It was almost as old as him. One day he set off with his young son to sell the horse before it died.They walked because the father did not want the horse to be too tired. They met two neighbours (邻居)on their way.The neighbours said, "Hello,why are
文摘In this paper numerical analysis of underground structures, taking account the transverse isotropy system of rocks, was done using CAST 3M code by varying the shape of excavation and the coefficient of earth pressure k. Numerical results reveal that the anisotropy behavior, the shape of hole and the coefficient of earth pressure k have significant influence to the mining induced stress field and rock deformations which directly control the stability of underground excavation design. The magnitude of horizontal stress obtained for the horse shoe shape excavation(25.2 MPa for k = 1; 52.7 MPa for k = 2)is lower than the magnitude obtained for circular hole(26.4 MPa for k = 1; 59.5 MPa for k = 2).Therefore, we have concluded that the horse shoe shape offers the best stability and the best design for engineer. The anisotropy system presented by rock mass can also influence the redistribution of stresses around hole opened. Numerical results have revealed that the magnitude of redistribution of horizontal stresses obtained for transverse isotropic rock(12.1 MPa for k = 0.5; 25.2 MPa for k = 1 and52.7 MPa for k = 2) is less than those obtained in the case of isotropic rock(27.6 MPa for k = 1;48.6 MPa for k = 2 and 90.81 MPa for k = 2). The more the rock has the anisotropic behavior, the more the mass of rock around the tunnel is stable.
文摘AIM： To evaluate the efficacy and safety of orbital decomposition （OD） surgery in combination with horse chestnut seed extract （HCSE）, as compared to OD atone, in patients with thyroid-associated ophthalmopathy （TAO）. METHODS： Sixty-two orbits from 62 TAO patients were randomly assigned to OD or OD＋HCSE at 1：1 ratio （31 received OD alone, 31 received OD ＋HCSE）. Forty-two orbits from 21 healthy subjects were used as controls. Complete ophthalmic examination and color Doppler flow imaging （CDFI） were performed before surgery and 3mo post-surgery on all 62 orbits from the TAO patients. CDFI were also performed on the 42 control orbits, The effect of OD ＋HCSE and OD alone on TAO orbits was compared on several endpoints, including superior ophthalmic vein blood flow （SOVBF） parameters, subjective assessment, soft tissue involvement, lid retraction, diplopia, eye movement restriction, degree of exophthalmos, and intraocular pressure. The control orbits were used as reference for the SOVBF parameters. RESULTS： OD surgery with or without HCSE improved SOVBF, symptoms and soft tissue involvement, decreased degree of exophthalmos and intraocular pressure in orbits of TAO patients. The OD ＋HCSE combination led to significantly better improvement of SOVBF than OD alone. The differences between the reductions of SOVBF in the two groups are 1.26 cmls in max-volecity and 0.52 cm/s in min-voiecity （P〈0.0001）. CONCLUSION： SOVBF is significantly reduced in the orbits affected with TAO, indicating that congestion may be an important factor contributing to TAO pathogenesis. OD surgery improves the SOVBF, and combination of HCSE medication and OD surgery further improved venous return than OD surgery alone.
基金the National Institutes of Health(1R01AG054839-01A1,1R41CA254500-01A1,and 1R21CA252360-01)for financial support of the research。
文摘Early-stage brain metastasis of breast cancer(BMBC), due to the existence of an intact blood–brain barrier(BBB), is one of the deadliest neurologic complications. To improve the efficacy of chemotherapy for BMBC, a Trojan horse strategy-based nanocarrier has been developed by integrating the cell membrane of a brain-homing cancer cell and a polymeric drug depot. With the camouflage of a MDA-MB-231/Br cell membrane, doxorubicin-loaded poly(D, L-lactic-co-glycolic acid) nanoparticle(DOX-PLGA@CM) shows enhanced cellular uptake and boosted killing potency for MDA-MB-231/Br cells. Furthermore, DOX-PLGA@CM is equipped with naturally selected molecules for BBB penetration, as evidenced by its boosted capacity in entering the brain of both healthy and early-stage BMBC mouse models. Consequently, DOX-PLGA@CM effectively reaches the metastatic tumor lesions in the brain, slows down cancer progression, reduces tumor burden, and extends the survival time for the BMBC animal.Furthermore, the simplicity and easy scale-up of the design opens a new window for the treatment of BMBC and other brain metastatic cancers.
文摘A cross sectional study was conducted from October 2010 to June 2011 in and around Gondar town to identify the species and determine prevalence of gastrointestinal parasites in donkeys and horses. Gross examination, direct fecal smear, sedimentation and floatation techniques were utilized to identify the eggs and larvae of parasites in feces. A total of 384 horses and donkeys were examined for gastrointestinal parasites. The overall prevalence of gastrointestinal parasites was 92.71% (356 from 384) with 80.95% (85 from 105) and 97.13% (271 from 279) in horses and donkeys, respectively. Prevalence of Strongyle, Parascaris equorum, Oxyuris equi, Gastrodiscus aegyptiacus and Gastrophilus intestinalis was 66.67%, 43.8%, 0.95%, 2.86%, and 0.95%, respectively in horses. Prevalence of gastrointestinal parasites was 87.81%, 42.29%, 4.30%, 5.73%, 1.43%, 3.58% and 0.72% for Strongyles, Parascaris equorum, Oxyuris equi, Fasciola, Tricuris, Gastrodiscus aegyptiacus and Gastrophilus intestinalis in donkeys, respectively. There was a statisticcally significant difference between species, housing and among feed types in prevalence of equine gastrointestinal parasites
基金Supported by the Chung-Ang University Graduate Research Scholarship in 2015Claire's Korea Research Fund(Seoul, Korea)。
文摘OBJECTIVE: To investigate the efficacy of horse oil on lipopolysaccharide(LPS)-induced inflammation in human keratinocytes.METHODS: Western blot analysis was performed to measure the expression of cyclooxygenase-2(COX-2) and IκBα. ELISA was used to analyze prostaglandin E2(PGE2) levels.RESULTS: Horse oil decreased LPS-induced COX-2 and PGE2 levels in a dose-dependent manner. Nuclear factor-kappa B(NF-κB) plays a key role in the expression of inflammatory cytokines and mediators. Therefore, we investigated the influence of horse oil on the NF-κB signaling pathways. Horse oil inhibited translocation of NF-κB from the cytosol to the nucleus. Furthermore, LPS-induced degradation of IκBα was recovered by horse oil. The activation of p38 mitogen-activated protein kinase(MAPK) reportedly induces degradation of IκBα In agreement with this, LPS activated p38 MAPK and caused IκBα degradation. Conversely, horse oil inhibited LPS-induced p38 MAPK activation and IκBαdegradation. In addition, a specific p38 MAPK inhibitor, SB203580, blocked IκBα degradation.CONCLUSION: Horse oil decreased COX-2 and PGE2 by inhibiting p38 MAPK activation, IκBα degradation, and the translocation of NF-κB.
基金supported by the National Key Research and the Development Project of China(2022YFD1800504)the Natural Science Foundation of Heilongjiang Province of China(TD2022C006)。
文摘African horse sickness(AHS)is an acute and fatal vectorborne infectious disease of equids,caused by the African horse sickness virus(AHSV).The World Organization for Animal Health(WOAH)has classified AHS as a notifiable animal disease,and AHS has also been classified as a Class I animal infectious disease in China.AHS is mainly found in Africa,the Middle East and the Arabian Peninsula.China is currently recognized by the WOAH as an AHS-free zone.
