BACKGROUND Propolis and honey are known for their antioxidant,hypoglycemic,and antiproteinuric effects.AIM To explore the effect of propolis,and honey,against D-glucose-induced hyperglycemia,acute kidney injury(AKI),l...BACKGROUND Propolis and honey are known for their antioxidant,hypoglycemic,and antiproteinuric effects.AIM To explore the effect of propolis,and honey,against D-glucose-induced hyperglycemia,acute kidney injury(AKI),liver injury,dyslipidemia,and changes in the oxidants and antioxidants in renal,hepatic,and pancreatic tissues.METHODS The chemical analysis and antioxidant content of propolis and honey and their effect on alpha-amylase and alpha-glucosidase activity were studied.The study included five groups of male rats;four groups(2,3,4 and 5)were treated with Dglucose,and one group was untreated,group 1.In addition to D-glucose,groups 3,4,and 5 were treated with propolis,honey,and their combination,respectively.Blood glucose levels,liver and renal function tests,urine protein and electrolytes,oxidant and antioxidant parameters,and histopathological changes in hepatic,renal,and pancreatic tissues were examined.RESULTS Propolis contains a higher level of total protein and exhibits a higher antioxidant activity.Honey has a higher alpha-amylase and glucosidase inhibitory activity than propolis.D-glucose caused a significant elevation of blood glucose,insulin,homeostasis model assessment,blood urea,creatinine,lipid parameters,liver enzymes,and urine protein levels.It significantly increases malondialdehyde and decreases antioxidant parameters in pancreatic,hepatic,and renal tissues.D-glucose caused histopathological changes in hepatic,renal,and pancreatic tissues;these changes were significantly ameliorated by honey and propolis.CONCLUSION Propolis,honey,or their combination treated hyperglycemia,AKI,proteinuria,liver injury,and dyslipidemia induced by D-glucose,most likely,through their antioxidant activity and alpha-amylase and alpha-glucosidase inhibitory activity.This will pave the way for testing this natural combination in the prevention of diabetic complications,as a complement to basic therapies.展开更多
[Objectives]To explore the methods for identifying pure honey.[Methods]Using 35 batches of Jiulongteng honey sourced from various production areas in Guangxi as the research subjects,this study investigated the chromo...[Objectives]To explore the methods for identifying pure honey.[Methods]Using 35 batches of Jiulongteng honey sourced from various production areas in Guangxi as the research subjects,this study investigated the chromogenic reactions of starch and dextrin,as well as the comparative study of thin-layer chromatography of oligosaccharides present in Jiulongteng honey.[Results]None of the 35 batches of Jiulongteng honey samples exhibited blue(indicating starch),green,or reddish-brown(indicating dextrin)coloration,suggesting that no adulterants such as artificially added starch,dextrin,or sugar were present in these samples.Furthermore,none of the 35 batches displayed additional spots below the corresponding positions of the control,indicating that the sugar composition was consistent with the oligosaccharide profile of natural honey.No components inconsistent with the oligosaccharide profile of natural honey were detected.Therefore,it can be concluded that the Jiulongteng honey samples in this experiment were pure and free from adulteration with starch,dextrin,or other sugar substances.[Conclusions]The method employed in this experiment is straightforward and quick to implement,effectively preventing adulterated honey from entering the market.It enhances the efficiency of quality control for Jiulongteng honey and promotes the healthy development of the Jiulongteng honey industry.展开更多
Honey trap is a really common type of stratagem which can be seen in many fields such as military,politics,diplomacy,and business.You can easily find many examples of it in the historical books of many different count...Honey trap is a really common type of stratagem which can be seen in many fields such as military,politics,diplomacy,and business.You can easily find many examples of it in the historical books of many different countries.As time went by,because of the changes of human thinking,just relying on the beautiful appearance could not ensure the success of a honey trap.Therefore,there appeared a new type of honey trap called personality grooming.As a matter of fact,the original form of personality grooming appeared a few centuries ago.However,at the very beginning,personality grooming was not directly related to honey trap.It is an interesting feature of honey trap that its forms will change due to the changes of the times.In this process,honey trap and personality grooming gradually integrate,developing a new type of honey trap.Compared with the traditional ones,this type of honey trap is much harder to guard against.展开更多
Honey,a natural substance,has long been valued for its dual role in both food and medicine in diverse cultural traditions,particularly in traditional Chinese medicine(TCM).It is rich in sugars,amino acids,enzymes,poly...Honey,a natural substance,has long been valued for its dual role in both food and medicine in diverse cultural traditions,particularly in traditional Chinese medicine(TCM).It is rich in sugars,amino acids,enzymes,polyphenols,and flavonoids that contribute to its antimicrobial,antioxidant,and immuno-modulatory properties.Additionally,honey is effective in managing some conditions,such as antibiotic-resistant infections,inflammation,and oxidative stress-related diseases.This review explores the extensive health benefits of honey,emphasizing the homology between food and medicine,as proposed by TCM philosophy.Further,this review explores the traditional applications of honey in respiratory health,wound healing,and gastrointestinal support,along with modern scientific validation of these uses.Moreover,the role of honey as a dietary supplement,functional food,and preservative in culinary practices is examined.Overall,this review highlights the synergy between ancient wisdom and contemporary science,advocating for the continued exploration of the role of honey in health,nutrition,and medicine.展开更多
A basic procedure for transforming readable data into encoded forms is encryption, which ensures security when the right decryption keys are used. Hadoop is susceptible to possible cyber-attacks because it lacks built...A basic procedure for transforming readable data into encoded forms is encryption, which ensures security when the right decryption keys are used. Hadoop is susceptible to possible cyber-attacks because it lacks built-in security measures, even though it can effectively handle and store enormous datasets using the Hadoop Distributed File System (HDFS). The increasing number of data breaches emphasizes how urgently creative encryption techniques are needed in cloud-based big data settings. This paper presents Adaptive Attribute-Based Honey Encryption (AABHE), a state-of-the-art technique that combines honey encryption with Ciphertext-Policy Attribute-Based Encryption (CP-ABE) to provide improved data security. Even if intercepted, AABHE makes sure that sensitive data cannot be accessed by unauthorized parties. With a focus on protecting huge files in HDFS, the suggested approach achieves 98% security robustness and 95% encryption efficiency, outperforming other encryption methods including Ciphertext-Policy Attribute-Based Encryption (CP-ABE), Key-Policy Attribute-Based Encryption (KB-ABE), and Advanced Encryption Standard combined with Attribute-Based Encryption (AES+ABE). By fixing Hadoop’s security flaws, AABHE fortifies its protections against data breaches and enhances Hadoop’s dependability as a platform for processing and storing massive amounts of data.展开更多
Honey, an apicultural product with a complex chemical composition, contains numerous bioactive compounds with potential antimicrobial effects. This study investigated the effect of Apis mellifera honey from Brazil’s ...Honey, an apicultural product with a complex chemical composition, contains numerous bioactive compounds with potential antimicrobial effects. This study investigated the effect of Apis mellifera honey from Brazil’s Central-West Region, combined with antibiotics, on bacterial membrane permeability, exploring the contributions of bioactive compounds and the botanical origin of honey. Six fresh Apis mellifera honey samples and their fractions (hexane and ethyl acetate) were analyzed, for a total of 18 samples. The bacteria Staphylococcus epidermidis, Helicobacter pylori and Enterococcus faecalis were used for antibacterial activity tests, which included minimum inhibitory concentration (MIC) determination and synergistic effect (checkerboard) assays. The total polyphenol and flavonoid contents were quantified, and the botanical origin was determined based on pollen analysis. The tested honey samples significantly affected bacterial membrane permeability when combined with rifampicin and clarithromycin. Although many honey-derived bioactive compounds, when isolated, did not exhibit significant activity against these bacteria, the additive or synergistic effect of multiple compounds acting on different targets appears to potentiate the antibacterial action. Descriptive statistical analysis, including means and 95% confidence intervals, confirmed the relevance of the findings. This study has provided an important discovery: Honey has an effect on bacterial membrane permeability, although the specific mechanisms involved in this process require further investigation.展开更多
Cancer occurs when the body’s cells grow beyond the usual control. Normal body cells multiply in a controlled manner and undergo apoptosis when the body no longer needs them. Different types of cancers exist, and the...Cancer occurs when the body’s cells grow beyond the usual control. Normal body cells multiply in a controlled manner and undergo apoptosis when the body no longer needs them. Different types of cancers exist, and the common ones include breast, cervical, prostate, lung, colon, and skin cancers. Several factors have been associated with cancers, and these factors include poor dieting, exposure to harmful chemicals and radiation, weak immune system, and genetics. Cancer presents an enormous health threat in the modern world and thus the need to identify an effective treatment. The conventional treatments used in the management of cancer include chemotherapy and physiotherapy. These forms of cancer treatments usually have enormous side effects on the subjects. In this respect, an alternative form of treatment would be effective in managing cancer patients. A substantial number of natural products have been observed to be effective in the management of cancer. These natural products include plants and other natural substances such as honey. This study focuses on the efficiency of natural products in the treatment of cancer. Also, the anticancer effects of Peganum harmala, Manuka honey, and Saudi honey will be analyzed. Bee honey and Peganum harmala have been traditionally used in the treatment of cancer. The extracts from Peganum harmala plant have also been shown to exhibit divers’ antitumor effects similar to the mode of action of a vast number of anticancer agents. These established hypotheses thus give the rationale for this study. In this experiment, extracts were obtained from Peganum harmala leaves and exposed to cervical, lung, and prostate cancer cells. Similarly, solutions of Manuka honey and Saudi honey were exposed to the cervical, lung, and prostate cancer cells. The experiment duration was 24 hours, which obtained results were recorded and analyzed. Peganum harmala extracts inhibit cancer cell growth at different and achievable concentrations. Manuka honey highly inhibits the growth of HeLa cancer cells while Saudi honey highly inhibits the growth of A549 cells. Peganum harmala can form an effective treatment for managing several types of cancers. Manuka Honey can be applied as an effective treatment for managing cervical cancer while Saudi honey can form an effective treatment for managing lung cancers.展开更多
Hybridizing metaheuristic algorithms involves synergistically combining different optimization techniques to effectively address complex and challenging optimization problems.This approach aims to leverage the strengt...Hybridizing metaheuristic algorithms involves synergistically combining different optimization techniques to effectively address complex and challenging optimization problems.This approach aims to leverage the strengths of multiple algorithms,enhancing solution quality,convergence speed,and robustness,thereby offering a more versatile and efficient means of solving intricate real-world optimization tasks.In this paper,we introduce a hybrid algorithm that amalgamates three distinct metaheuristics:the Beluga Whale Optimization(BWO),the Honey Badger Algorithm(HBA),and the Jellyfish Search(JS)optimizer.The proposed hybrid algorithm will be referred to as BHJO.Through this fusion,the BHJO algorithm aims to leverage the strengths of each optimizer.Before this hybridization,we thoroughly examined the exploration and exploitation capabilities of the BWO,HBA,and JS metaheuristics,as well as their ability to strike a balance between exploration and exploitation.This meticulous analysis allowed us to identify the pros and cons of each algorithm,enabling us to combine them in a novel hybrid approach that capitalizes on their respective strengths for enhanced optimization performance.In addition,the BHJO algorithm incorporates Opposition-Based Learning(OBL)to harness the advantages offered by this technique,leveraging its diverse exploration,accelerated convergence,and improved solution quality to enhance the overall performance and effectiveness of the hybrid algorithm.Moreover,the performance of the BHJO algorithm was evaluated across a range of both unconstrained and constrained optimization problems,providing a comprehensive assessment of its efficacy and applicability in diverse problem domains.Similarly,the BHJO algorithm was subjected to a comparative analysis with several renowned algorithms,where mean and standard deviation values were utilized as evaluation metrics.This rigorous comparison aimed to assess the performance of the BHJOalgorithmabout its counterparts,shedding light on its effectiveness and reliability in solving optimization problems.Finally,the obtained numerical statistics underwent rigorous analysis using the Friedman post hoc Dunn’s test.The resulting numerical values revealed the BHJO algorithm’s competitiveness in tackling intricate optimization problems,affirming its capability to deliver favorable outcomes in challenging scenarios.展开更多
[Objectives]To explore the effect of storage time on pH and 5-hydroxymethylfurfural content in Jiulongteng honey.[Methods]The pH of Jiulongteng honey was determined by neutralization titration with sodium hydroxide st...[Objectives]To explore the effect of storage time on pH and 5-hydroxymethylfurfural content in Jiulongteng honey.[Methods]The pH of Jiulongteng honey was determined by neutralization titration with sodium hydroxide standard solution.The content of 5-hydroxymethylfurfural in Jiulongteng honey was determined by HPLC.Chromatographic conditions:ZORBAX SB-C 18 column(250 mm×4.6 mm,5μm)from Agilent Co.,Ltd.,acetonitrile-0.1%formic acid solution(5:95)as mobile phase,flow rate of 0.8 mL/min,5-hydroxymethylfurfural detection wavelength of 284 nm,guanosine detection wavelength of 254 nm.[Results]The pH of 12 batches of Jiulongteng honey was 3.70-3.84 in the new honey stage,3.92-4.05 in the old honey stage 1,and 4.25-4.53 in the old honey stage 2;5-hydroxymethylfurfural was not detected in the new honey stage,5-hydroxymethylfurfural was detected in FM-001 in the old honey stage 1,and 5-hydroxymethylfurfural was detected in most samples in the old honey stage 2.[Conclusions]The pH and 5-hydroxymethylfurfural content of 12 batches of Jiulongteng honey met the requirements within 3 years of storage.There was no 5-hydroxymethylfurfural in Jiulongteng honey,but with the extension of storage time,the detection amount of 5-hydroxymethylfurfural increased significantly even if Jiulongteng honey was stored at low temperature.Therefore,5-hydroxymethylfurfural can be used as an important indicator of honey freshness.展开更多
BACKGROUND Diabetes is known damage the liver and kidney,leading to hepatic dysfunction and kidney failure.Honey is believed to help in lowering the blood glucose levels of diabetic patients and reducing diabetic comp...BACKGROUND Diabetes is known damage the liver and kidney,leading to hepatic dysfunction and kidney failure.Honey is believed to help in lowering the blood glucose levels of diabetic patients and reducing diabetic complications.However,the effect of stingless bee honey(SBH)administration in relieving liver and kidney damage in diabetes has not been well-studied.AIM To investigate the effect of SBH administration on the kidney and liver of streptozotocin-induced(STZ;55 mg/kg)diabetic Sprague Dawley rats.METHODS The rats were grouped as follows(n=6 per group):non-diabetic(ND),untreated diabetic(UNT),metformin-treated(MET),and SBH+metformin-treated(SBME)groups.After successful diabetic induction,ND and UNT rats were given normal saline,whereas the treatment groups received SBH(2.0 g/kg and/or metformin(250 mg/kg)for 12 d.Serum biochemical parameters and histological changes using hematoxylin and eosin(H&E)and periodic acid–Schiff(PAS)staining were evaluated.RESULTS On H&E and PAS staining,the ND group showed normal architecture and cellularity of Bowman’s capsule and tubules,whereas the UNT and MET groups had an increased glomerular cellularity and thickened basement membrane.The SBH-treated group showed a decrease in hydropic changes and mild cellularity of the glomerulus vs the ND group based on H&E staining,but the two were similar on PAS staining.Likewise,the SBME-treated group had an increase in cellularity of the glomerulus on H&E staining,but it was comparable to the SBH and ND groups on PAS staining.UNT diabetic rats had tubular hydropic tubules,which were smaller than other groups.Reduced fatty vacuole formation and dilated blood sinusoids in liver tissue were seen in the SBH group.Conversely,the UNT group had high glucose levels,which subsequently increased MDA levels,ultimately leading to liver damage.SBH treatment reduced this damage,as evidenced by having the lowest fasting glucose,serum alanine transaminase,aspartate transaminase,and alkaline phosphatase levels compared to other groups,although the levels of liver enzymes were not statistically significant.CONCLUSION The cellularity of the Bowman’s capsule,as well as histological alteration of kidney tubules,glomerular membranes,and liver tissues in diabetic rats after oral SBH resembled those of ND rats.Therefore,SBH exhibited a protective hepatorenal effect in a diabetic rat model.展开更多
The study evaluated the effectiveness of honey dressings in healing recent skin wounds with soft tissue loss on limbs. Conducted over a year with 60 patients, results showed a significant reduction in wound size and e...The study evaluated the effectiveness of honey dressings in healing recent skin wounds with soft tissue loss on limbs. Conducted over a year with 60 patients, results showed a significant reduction in wound size and early healing, with complete recovery on average within 29 days. The study supports honey as a viable wound treatment.展开更多
Purpose: Telomere length (TL) is an indicator of age;however, hormonal influences complicate individual aging. It remains unclear whether TL shortening is a direct factor in both individual and cellular aging. Therefo...Purpose: Telomere length (TL) is an indicator of age;however, hormonal influences complicate individual aging. It remains unclear whether TL shortening is a direct factor in both individual and cellular aging. Therefore, we examined the direct relationship between TL and cellular senescence at the cellular level. Methods: Telomerase activity, TL, and gene expression were measured in cultured human lung-, fetal-, and skin-derived fibroblasts, human skin keratinocytes, and telomerase reverse transcriptase (TERT) gene-immortalized cells using detection kits, Cawthon’s method, and reverse transcription-quantitative polymerase chain reaction, respectively. Novel substances that elongate telomeres were screened to confirm cell rejuvenation effects. Results: Long-term cell culture of TIG-1-20 normal human fibroblasts resulted in TL shortening, decreased division rate, and senescence progression, whereas in OUMS-36T-2 cells, TL elongation via TERT gene transfer increased the division rate, reduced endoplasmic reticulum stress, and upregulated genes associated with young individuals, indicating that cellular rejuvenation occurs via TL elongation. In addition, a honey child powder (HCP) extract was found through screening, and the HCP extract strongly suppressed the menin gene, resulting in increased telomerase activity and extended cell lifespan. Upon addition of the HCP extract to skin fibroblasts, gene expression of moisturizing components, including collagen, hyaluronic acid, and elastin, increased, and exhibited a rejuvenating effect with an increase in elastin amount. Conclusions: TL elongation or shortening is involved in cell proliferation rate and cellular aging, and TL elongation rejuvenates cells. In addition, HCP extract has a rejuvenating effect on cells and is expected to be a rejuvenating compound.展开更多
Honey harvesting throughout beekeeping is a technology recently introduced in Gabon comparing to the other African countries well known in international honey trade. The purpose of this study is to contribute to the k...Honey harvesting throughout beekeeping is a technology recently introduced in Gabon comparing to the other African countries well known in international honey trade. The purpose of this study is to contribute to the knowledge of the quality of multi-flower honeys from the savannah-gallery forest complex and primary forest not well documented in Gabon. Physical and chemical parameters of different honeys were analyzed using the official methods of analysis of the Association of Official Analytical Chemists. The results of physico-chemical analysis show variations in pH (3.4 to 3.9), free acidity (37.12 to 76.65 meq∙kg−1), water content (17.49% to 21.21%), electrical conductivity (0.64 to 1.24 mS∙cm−1), density (1.02 to 1.03) and total sugar content (77% to 82%). These variations were significantly different (p < 0.05) between certain honeys, but independent of the type of ecosystem considered. The pH values confirmed the botanical origin of the five honeys. The physico-chemical parameters of the honeys show significant correlations (p < 0.05). Moisture content is negatively correlated with conductivity (r = −0.628), pH (r = −0.631), density (r = −0.552) and total sugar content (r = −0.890). Conductivity is positively correlated with free acidity (r = 0.688) and total sugar content (r = 0.776), and negatively correlated with water content (r = −0.628). All honeys were in line with Codex Alimentarius standards, except for 3 types of honey (M1, M2 and M4) for free acidity and electrical conductivity, showing possible fermentation of these honeys.展开更多
Honey has long been considered a wound treatment used to keep cuts and other epidermal injuries clean. This study tested that claim by comparing manuka honey used in medicine today, local unprocessed honey taken strai...Honey has long been considered a wound treatment used to keep cuts and other epidermal injuries clean. This study tested that claim by comparing manuka honey used in medicine today, local unprocessed honey taken straight from a hive, and pasteurized honey found at a store, on strains of E. coli and S. epidermidis. The study evaluated the effects these honeys had on bacterial growth to determine which had the greatest inhibition of bacterial growth. To determine this, plates streaked with strains of E. coli or S. epidermidis bacteria and agar wells filled with one of the honeys were incubated and subsequently the diameter of the zone of inhibition was measured. After 20 trials using each honey and bacteria type, manuka and unprocessed were shown to have a statistically significant advantage over the pasteurized honey at inhibiting the growth of E. coli and S. epidermidis, though it was variable whether manuka had an advantage over the unprocessed honey.展开更多
[Objective] The experiment aimed to study chromatographic fingerprint in volatile components of acacia honey and provide scientific evaluation and effective control on quality of acacia honey.[Method] Using solid-phas...[Objective] The experiment aimed to study chromatographic fingerprint in volatile components of acacia honey and provide scientific evaluation and effective control on quality of acacia honey.[Method] Using solid-phase microextraction method to separate and detect volatile components and construct chromatographic fingerprint.[Result] The honey was preheated for 15 min in water bath at 40 ℃ and solid-phase microextraction 85 μmPA was used to extract in overhead air about 30 min,then put it into the injector and desorpted 3 min,which is in 230 ℃.The Supelco WaxTM10 30 m×0.25 mm×0.25 μm column and gradient heating program was the best method to separate volatile components from honey.83 fingerprint peaks were constructed,among which 17 common fingerprint peaks were comprised of chromatographic fingerprint of volatile components of acacia honey.[Conclusion] The chromatographic fingerprint could provide reference for quality control of acacia honey.展开更多
In order to build method for measuring of elements in honey, the contents of Ba, Cu, Pb, Cd, As, Hg, Cr, Mn, Se had been determined by microwave diges- tion （MWD） and actively coupled plasma atomic emission spectrom...In order to build method for measuring of elements in honey, the contents of Ba, Cu, Pb, Cd, As, Hg, Cr, Mn, Se had been determined by microwave diges- tion （MWD） and actively coupled plasma atomic emission spectrometry （ICP-AES）. The results show that, the range of heavy metal elements were in the range of 0- 10.0 mg/L, with good linear relationship. In addition, all correlation coefficients kept higher than 0.999, and the recovery rate of ample added with standard solution was in 94.1%-110%. By comparison with the result of chemical experiment, the method was quickly accessible and convenient, widely used in the measurement of elements in honey, with satisfactory results.展开更多
[Objective] The aim was to establish a rapid method for the determination of the content of total flavonoids in the honey comb. [Method] The content of total flavonoids weas determined based on samples including comb ...[Objective] The aim was to establish a rapid method for the determination of the content of total flavonoids in the honey comb. [Method] The content of total flavonoids weas determined based on samples including comb honey, propolis, royal jelly, linden tree honey, with a polyamide column chromatography by spectrophotometry at 360 nm wavelength. [Result] The recovery rate of the method was above 90%, and the variation coefficient was lower than 2%. Compared with the traditional method, this method has less reagent consumption, simple operation and so on.[Conclusion] The content of total flavonoids in comb honey was 14.97 mg/100 g, although is only 0.87% of the total flavone content in propolis. The contained total flavonoids at least doubled than that of others and comb honey is more valuable in the content of flavonoids.展开更多
Quality analysis was performed to major cultivars, ‘Hongxin pomelo’ and ‘Wuzi pomelo＇ with uniform maturity. Total soluble solid （TSS）, titratable acid, vita- min C, juice yield, edible rate, single-fruit weight...Quality analysis was performed to major cultivars, ‘Hongxin pomelo’ and ‘Wuzi pomelo＇ with uniform maturity. Total soluble solid （TSS）, titratable acid, vita- min C, juice yield, edible rate, single-fruit weight and pesticide residues of the 2 cultivars were detected. The results showed that： the TSS contents of ‘Hongxin pomelo＇ and ‘Wuzi pomelo＇ were in the range of 8%-12%, the vitamin C contents were in the range of 0.3-0.6 g/L, the juice yields were in the range of 56%-68%, the edible rates were in the range of 52%-68%, and the single-fruit weights were in the range of 1-2.5 kg. The titratable acid content was higher in ‘Hongxin pomelo＇ than in ‘Wuzi pomelo＇. Only Dursban and imidacloprid were detected at low con- centrations in pericarp, and the residues of the 3 pesticides were all lower than their detection limits. Therefore, honey pomelo produced in Hainan all could be ate safely.展开更多
[Objective] The aim was to analyze content of flavones in honey of pomegranate flowers. [Method] Contents of total flavones in 24 honey samples of pomegranate flowers from Mengzi City in 2011 and 2012 were measured as...[Objective] The aim was to analyze content of flavones in honey of pomegranate flowers. [Method] Contents of total flavones in 24 honey samples of pomegranate flowers from Mengzi City in 2011 and 2012 were measured as per- spectrophotometry method with acetone as an extractant and rutin as a control. [Result] The average content of flavones in honey of pomegranate flowers was at （2.36±1.36）mg/100 g in 2011 and significantly higher than that in 2012 at （1.71± 0.53）mg/100 g. Analysis was conducted on flavones content in different batches and the results indicated that content of flavones in honey in different batches changed from increasing to decreasing, namely, the content was maximal in peak flowering stage, followed by late flowering stage and initial flowering stage. [Conclusion] The research provides references for development, use and function exploration of the honey of pomegranate flowers.展开更多
文摘BACKGROUND Propolis and honey are known for their antioxidant,hypoglycemic,and antiproteinuric effects.AIM To explore the effect of propolis,and honey,against D-glucose-induced hyperglycemia,acute kidney injury(AKI),liver injury,dyslipidemia,and changes in the oxidants and antioxidants in renal,hepatic,and pancreatic tissues.METHODS The chemical analysis and antioxidant content of propolis and honey and their effect on alpha-amylase and alpha-glucosidase activity were studied.The study included five groups of male rats;four groups(2,3,4 and 5)were treated with Dglucose,and one group was untreated,group 1.In addition to D-glucose,groups 3,4,and 5 were treated with propolis,honey,and their combination,respectively.Blood glucose levels,liver and renal function tests,urine protein and electrolytes,oxidant and antioxidant parameters,and histopathological changes in hepatic,renal,and pancreatic tissues were examined.RESULTS Propolis contains a higher level of total protein and exhibits a higher antioxidant activity.Honey has a higher alpha-amylase and glucosidase inhibitory activity than propolis.D-glucose caused a significant elevation of blood glucose,insulin,homeostasis model assessment,blood urea,creatinine,lipid parameters,liver enzymes,and urine protein levels.It significantly increases malondialdehyde and decreases antioxidant parameters in pancreatic,hepatic,and renal tissues.D-glucose caused histopathological changes in hepatic,renal,and pancreatic tissues;these changes were significantly ameliorated by honey and propolis.CONCLUSION Propolis,honey,or their combination treated hyperglycemia,AKI,proteinuria,liver injury,and dyslipidemia induced by D-glucose,most likely,through their antioxidant activity and alpha-amylase and alpha-glucosidase inhibitory activity.This will pave the way for testing this natural combination in the prevention of diabetic complications,as a complement to basic therapies.
基金Supported by Enhancement Project of Basic Scientific Research Ability of Young and Middle-aged Teachers in Guangxi Universities(2020KY07040)School-level Scientific Research Project of Guangxi University of Chinese Medicine(2024QN022)Self-financed Scientific Research Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(GXZYL20240818).
文摘[Objectives]To explore the methods for identifying pure honey.[Methods]Using 35 batches of Jiulongteng honey sourced from various production areas in Guangxi as the research subjects,this study investigated the chromogenic reactions of starch and dextrin,as well as the comparative study of thin-layer chromatography of oligosaccharides present in Jiulongteng honey.[Results]None of the 35 batches of Jiulongteng honey samples exhibited blue(indicating starch),green,or reddish-brown(indicating dextrin)coloration,suggesting that no adulterants such as artificially added starch,dextrin,or sugar were present in these samples.Furthermore,none of the 35 batches displayed additional spots below the corresponding positions of the control,indicating that the sugar composition was consistent with the oligosaccharide profile of natural honey.No components inconsistent with the oligosaccharide profile of natural honey were detected.Therefore,it can be concluded that the Jiulongteng honey samples in this experiment were pure and free from adulteration with starch,dextrin,or other sugar substances.[Conclusions]The method employed in this experiment is straightforward and quick to implement,effectively preventing adulterated honey from entering the market.It enhances the efficiency of quality control for Jiulongteng honey and promotes the healthy development of the Jiulongteng honey industry.
文摘Honey trap is a really common type of stratagem which can be seen in many fields such as military,politics,diplomacy,and business.You can easily find many examples of it in the historical books of many different countries.As time went by,because of the changes of human thinking,just relying on the beautiful appearance could not ensure the success of a honey trap.Therefore,there appeared a new type of honey trap called personality grooming.As a matter of fact,the original form of personality grooming appeared a few centuries ago.However,at the very beginning,personality grooming was not directly related to honey trap.It is an interesting feature of honey trap that its forms will change due to the changes of the times.In this process,honey trap and personality grooming gradually integrate,developing a new type of honey trap.Compared with the traditional ones,this type of honey trap is much harder to guard against.
基金supported by the National Natural Science Foundation of China(32322079)Beijing Nova Program(20220484101)Talented Young Scientist Program,Ministry of Science and Technology of the People's Republic of China,and China Postdoctoral Science Foundation(2024M753576).
文摘Honey,a natural substance,has long been valued for its dual role in both food and medicine in diverse cultural traditions,particularly in traditional Chinese medicine(TCM).It is rich in sugars,amino acids,enzymes,polyphenols,and flavonoids that contribute to its antimicrobial,antioxidant,and immuno-modulatory properties.Additionally,honey is effective in managing some conditions,such as antibiotic-resistant infections,inflammation,and oxidative stress-related diseases.This review explores the extensive health benefits of honey,emphasizing the homology between food and medicine,as proposed by TCM philosophy.Further,this review explores the traditional applications of honey in respiratory health,wound healing,and gastrointestinal support,along with modern scientific validation of these uses.Moreover,the role of honey as a dietary supplement,functional food,and preservative in culinary practices is examined.Overall,this review highlights the synergy between ancient wisdom and contemporary science,advocating for the continued exploration of the role of honey in health,nutrition,and medicine.
基金funded by Princess Nourah bint Abdulrahman UniversityResearchers Supporting Project number (PNURSP2024R408), Princess Nourah bint AbdulrahmanUniversity, Riyadh, Saudi Arabia.
文摘A basic procedure for transforming readable data into encoded forms is encryption, which ensures security when the right decryption keys are used. Hadoop is susceptible to possible cyber-attacks because it lacks built-in security measures, even though it can effectively handle and store enormous datasets using the Hadoop Distributed File System (HDFS). The increasing number of data breaches emphasizes how urgently creative encryption techniques are needed in cloud-based big data settings. This paper presents Adaptive Attribute-Based Honey Encryption (AABHE), a state-of-the-art technique that combines honey encryption with Ciphertext-Policy Attribute-Based Encryption (CP-ABE) to provide improved data security. Even if intercepted, AABHE makes sure that sensitive data cannot be accessed by unauthorized parties. With a focus on protecting huge files in HDFS, the suggested approach achieves 98% security robustness and 95% encryption efficiency, outperforming other encryption methods including Ciphertext-Policy Attribute-Based Encryption (CP-ABE), Key-Policy Attribute-Based Encryption (KB-ABE), and Advanced Encryption Standard combined with Attribute-Based Encryption (AES+ABE). By fixing Hadoop’s security flaws, AABHE fortifies its protections against data breaches and enhances Hadoop’s dependability as a platform for processing and storing massive amounts of data.
文摘Honey, an apicultural product with a complex chemical composition, contains numerous bioactive compounds with potential antimicrobial effects. This study investigated the effect of Apis mellifera honey from Brazil’s Central-West Region, combined with antibiotics, on bacterial membrane permeability, exploring the contributions of bioactive compounds and the botanical origin of honey. Six fresh Apis mellifera honey samples and their fractions (hexane and ethyl acetate) were analyzed, for a total of 18 samples. The bacteria Staphylococcus epidermidis, Helicobacter pylori and Enterococcus faecalis were used for antibacterial activity tests, which included minimum inhibitory concentration (MIC) determination and synergistic effect (checkerboard) assays. The total polyphenol and flavonoid contents were quantified, and the botanical origin was determined based on pollen analysis. The tested honey samples significantly affected bacterial membrane permeability when combined with rifampicin and clarithromycin. Although many honey-derived bioactive compounds, when isolated, did not exhibit significant activity against these bacteria, the additive or synergistic effect of multiple compounds acting on different targets appears to potentiate the antibacterial action. Descriptive statistical analysis, including means and 95% confidence intervals, confirmed the relevance of the findings. This study has provided an important discovery: Honey has an effect on bacterial membrane permeability, although the specific mechanisms involved in this process require further investigation.
文摘Cancer occurs when the body’s cells grow beyond the usual control. Normal body cells multiply in a controlled manner and undergo apoptosis when the body no longer needs them. Different types of cancers exist, and the common ones include breast, cervical, prostate, lung, colon, and skin cancers. Several factors have been associated with cancers, and these factors include poor dieting, exposure to harmful chemicals and radiation, weak immune system, and genetics. Cancer presents an enormous health threat in the modern world and thus the need to identify an effective treatment. The conventional treatments used in the management of cancer include chemotherapy and physiotherapy. These forms of cancer treatments usually have enormous side effects on the subjects. In this respect, an alternative form of treatment would be effective in managing cancer patients. A substantial number of natural products have been observed to be effective in the management of cancer. These natural products include plants and other natural substances such as honey. This study focuses on the efficiency of natural products in the treatment of cancer. Also, the anticancer effects of Peganum harmala, Manuka honey, and Saudi honey will be analyzed. Bee honey and Peganum harmala have been traditionally used in the treatment of cancer. The extracts from Peganum harmala plant have also been shown to exhibit divers’ antitumor effects similar to the mode of action of a vast number of anticancer agents. These established hypotheses thus give the rationale for this study. In this experiment, extracts were obtained from Peganum harmala leaves and exposed to cervical, lung, and prostate cancer cells. Similarly, solutions of Manuka honey and Saudi honey were exposed to the cervical, lung, and prostate cancer cells. The experiment duration was 24 hours, which obtained results were recorded and analyzed. Peganum harmala extracts inhibit cancer cell growth at different and achievable concentrations. Manuka honey highly inhibits the growth of HeLa cancer cells while Saudi honey highly inhibits the growth of A549 cells. Peganum harmala can form an effective treatment for managing several types of cancers. Manuka Honey can be applied as an effective treatment for managing cervical cancer while Saudi honey can form an effective treatment for managing lung cancers.
基金funded by the Researchers Supporting Program at King Saud University(RSPD2024R809).
文摘Hybridizing metaheuristic algorithms involves synergistically combining different optimization techniques to effectively address complex and challenging optimization problems.This approach aims to leverage the strengths of multiple algorithms,enhancing solution quality,convergence speed,and robustness,thereby offering a more versatile and efficient means of solving intricate real-world optimization tasks.In this paper,we introduce a hybrid algorithm that amalgamates three distinct metaheuristics:the Beluga Whale Optimization(BWO),the Honey Badger Algorithm(HBA),and the Jellyfish Search(JS)optimizer.The proposed hybrid algorithm will be referred to as BHJO.Through this fusion,the BHJO algorithm aims to leverage the strengths of each optimizer.Before this hybridization,we thoroughly examined the exploration and exploitation capabilities of the BWO,HBA,and JS metaheuristics,as well as their ability to strike a balance between exploration and exploitation.This meticulous analysis allowed us to identify the pros and cons of each algorithm,enabling us to combine them in a novel hybrid approach that capitalizes on their respective strengths for enhanced optimization performance.In addition,the BHJO algorithm incorporates Opposition-Based Learning(OBL)to harness the advantages offered by this technique,leveraging its diverse exploration,accelerated convergence,and improved solution quality to enhance the overall performance and effectiveness of the hybrid algorithm.Moreover,the performance of the BHJO algorithm was evaluated across a range of both unconstrained and constrained optimization problems,providing a comprehensive assessment of its efficacy and applicability in diverse problem domains.Similarly,the BHJO algorithm was subjected to a comparative analysis with several renowned algorithms,where mean and standard deviation values were utilized as evaluation metrics.This rigorous comparison aimed to assess the performance of the BHJOalgorithmabout its counterparts,shedding light on its effectiveness and reliability in solving optimization problems.Finally,the obtained numerical statistics underwent rigorous analysis using the Friedman post hoc Dunn’s test.The resulting numerical values revealed the BHJO algorithm’s competitiveness in tackling intricate optimization problems,affirming its capability to deliver favorable outcomes in challenging scenarios.
基金Supported by Young and Middle-aged Teachers Scientific Research Basic Ability Improvement Project in Universities of Guangxi in 2020 (2020 KY07040)School-level Scientific Research Project of Guangxi University of Chinese Medicine in 2021 (2021MS010).
文摘[Objectives]To explore the effect of storage time on pH and 5-hydroxymethylfurfural content in Jiulongteng honey.[Methods]The pH of Jiulongteng honey was determined by neutralization titration with sodium hydroxide standard solution.The content of 5-hydroxymethylfurfural in Jiulongteng honey was determined by HPLC.Chromatographic conditions:ZORBAX SB-C 18 column(250 mm×4.6 mm,5μm)from Agilent Co.,Ltd.,acetonitrile-0.1%formic acid solution(5:95)as mobile phase,flow rate of 0.8 mL/min,5-hydroxymethylfurfural detection wavelength of 284 nm,guanosine detection wavelength of 254 nm.[Results]The pH of 12 batches of Jiulongteng honey was 3.70-3.84 in the new honey stage,3.92-4.05 in the old honey stage 1,and 4.25-4.53 in the old honey stage 2;5-hydroxymethylfurfural was not detected in the new honey stage,5-hydroxymethylfurfural was detected in FM-001 in the old honey stage 1,and 5-hydroxymethylfurfural was detected in most samples in the old honey stage 2.[Conclusions]The pH and 5-hydroxymethylfurfural content of 12 batches of Jiulongteng honey met the requirements within 3 years of storage.There was no 5-hydroxymethylfurfural in Jiulongteng honey,but with the extension of storage time,the detection amount of 5-hydroxymethylfurfural increased significantly even if Jiulongteng honey was stored at low temperature.Therefore,5-hydroxymethylfurfural can be used as an important indicator of honey freshness.
基金Ministry of Higher Education,Malaysia for Fundamental Research Grant Scheme FRGS/1/2019/SKK06/USM/03/6,No.291983-329281.
文摘BACKGROUND Diabetes is known damage the liver and kidney,leading to hepatic dysfunction and kidney failure.Honey is believed to help in lowering the blood glucose levels of diabetic patients and reducing diabetic complications.However,the effect of stingless bee honey(SBH)administration in relieving liver and kidney damage in diabetes has not been well-studied.AIM To investigate the effect of SBH administration on the kidney and liver of streptozotocin-induced(STZ;55 mg/kg)diabetic Sprague Dawley rats.METHODS The rats were grouped as follows(n=6 per group):non-diabetic(ND),untreated diabetic(UNT),metformin-treated(MET),and SBH+metformin-treated(SBME)groups.After successful diabetic induction,ND and UNT rats were given normal saline,whereas the treatment groups received SBH(2.0 g/kg and/or metformin(250 mg/kg)for 12 d.Serum biochemical parameters and histological changes using hematoxylin and eosin(H&E)and periodic acid–Schiff(PAS)staining were evaluated.RESULTS On H&E and PAS staining,the ND group showed normal architecture and cellularity of Bowman’s capsule and tubules,whereas the UNT and MET groups had an increased glomerular cellularity and thickened basement membrane.The SBH-treated group showed a decrease in hydropic changes and mild cellularity of the glomerulus vs the ND group based on H&E staining,but the two were similar on PAS staining.Likewise,the SBME-treated group had an increase in cellularity of the glomerulus on H&E staining,but it was comparable to the SBH and ND groups on PAS staining.UNT diabetic rats had tubular hydropic tubules,which were smaller than other groups.Reduced fatty vacuole formation and dilated blood sinusoids in liver tissue were seen in the SBH group.Conversely,the UNT group had high glucose levels,which subsequently increased MDA levels,ultimately leading to liver damage.SBH treatment reduced this damage,as evidenced by having the lowest fasting glucose,serum alanine transaminase,aspartate transaminase,and alkaline phosphatase levels compared to other groups,although the levels of liver enzymes were not statistically significant.CONCLUSION The cellularity of the Bowman’s capsule,as well as histological alteration of kidney tubules,glomerular membranes,and liver tissues in diabetic rats after oral SBH resembled those of ND rats.Therefore,SBH exhibited a protective hepatorenal effect in a diabetic rat model.
文摘The study evaluated the effectiveness of honey dressings in healing recent skin wounds with soft tissue loss on limbs. Conducted over a year with 60 patients, results showed a significant reduction in wound size and early healing, with complete recovery on average within 29 days. The study supports honey as a viable wound treatment.
文摘Purpose: Telomere length (TL) is an indicator of age;however, hormonal influences complicate individual aging. It remains unclear whether TL shortening is a direct factor in both individual and cellular aging. Therefore, we examined the direct relationship between TL and cellular senescence at the cellular level. Methods: Telomerase activity, TL, and gene expression were measured in cultured human lung-, fetal-, and skin-derived fibroblasts, human skin keratinocytes, and telomerase reverse transcriptase (TERT) gene-immortalized cells using detection kits, Cawthon’s method, and reverse transcription-quantitative polymerase chain reaction, respectively. Novel substances that elongate telomeres were screened to confirm cell rejuvenation effects. Results: Long-term cell culture of TIG-1-20 normal human fibroblasts resulted in TL shortening, decreased division rate, and senescence progression, whereas in OUMS-36T-2 cells, TL elongation via TERT gene transfer increased the division rate, reduced endoplasmic reticulum stress, and upregulated genes associated with young individuals, indicating that cellular rejuvenation occurs via TL elongation. In addition, a honey child powder (HCP) extract was found through screening, and the HCP extract strongly suppressed the menin gene, resulting in increased telomerase activity and extended cell lifespan. Upon addition of the HCP extract to skin fibroblasts, gene expression of moisturizing components, including collagen, hyaluronic acid, and elastin, increased, and exhibited a rejuvenating effect with an increase in elastin amount. Conclusions: TL elongation or shortening is involved in cell proliferation rate and cellular aging, and TL elongation rejuvenates cells. In addition, HCP extract has a rejuvenating effect on cells and is expected to be a rejuvenating compound.
文摘Honey harvesting throughout beekeeping is a technology recently introduced in Gabon comparing to the other African countries well known in international honey trade. The purpose of this study is to contribute to the knowledge of the quality of multi-flower honeys from the savannah-gallery forest complex and primary forest not well documented in Gabon. Physical and chemical parameters of different honeys were analyzed using the official methods of analysis of the Association of Official Analytical Chemists. The results of physico-chemical analysis show variations in pH (3.4 to 3.9), free acidity (37.12 to 76.65 meq∙kg−1), water content (17.49% to 21.21%), electrical conductivity (0.64 to 1.24 mS∙cm−1), density (1.02 to 1.03) and total sugar content (77% to 82%). These variations were significantly different (p < 0.05) between certain honeys, but independent of the type of ecosystem considered. The pH values confirmed the botanical origin of the five honeys. The physico-chemical parameters of the honeys show significant correlations (p < 0.05). Moisture content is negatively correlated with conductivity (r = −0.628), pH (r = −0.631), density (r = −0.552) and total sugar content (r = −0.890). Conductivity is positively correlated with free acidity (r = 0.688) and total sugar content (r = 0.776), and negatively correlated with water content (r = −0.628). All honeys were in line with Codex Alimentarius standards, except for 3 types of honey (M1, M2 and M4) for free acidity and electrical conductivity, showing possible fermentation of these honeys.
文摘Honey has long been considered a wound treatment used to keep cuts and other epidermal injuries clean. This study tested that claim by comparing manuka honey used in medicine today, local unprocessed honey taken straight from a hive, and pasteurized honey found at a store, on strains of E. coli and S. epidermidis. The study evaluated the effects these honeys had on bacterial growth to determine which had the greatest inhibition of bacterial growth. To determine this, plates streaked with strains of E. coli or S. epidermidis bacteria and agar wells filled with one of the honeys were incubated and subsequently the diameter of the zone of inhibition was measured. After 20 trials using each honey and bacteria type, manuka and unprocessed were shown to have a statistically significant advantage over the pasteurized honey at inhibiting the growth of E. coli and S. epidermidis, though it was variable whether manuka had an advantage over the unprocessed honey.
基金Support by Department of Education Science and Technology Research Projects of Hebei Province(2008310)the National Special Fund for the Commonweal Industry(200810345)~~
文摘[Objective] The experiment aimed to study chromatographic fingerprint in volatile components of acacia honey and provide scientific evaluation and effective control on quality of acacia honey.[Method] Using solid-phase microextraction method to separate and detect volatile components and construct chromatographic fingerprint.[Result] The honey was preheated for 15 min in water bath at 40 ℃ and solid-phase microextraction 85 μmPA was used to extract in overhead air about 30 min,then put it into the injector and desorpted 3 min,which is in 230 ℃.The Supelco WaxTM10 30 m×0.25 mm×0.25 μm column and gradient heating program was the best method to separate volatile components from honey.83 fingerprint peaks were constructed,among which 17 common fingerprint peaks were comprised of chromatographic fingerprint of volatile components of acacia honey.[Conclusion] The chromatographic fingerprint could provide reference for quality control of acacia honey.
文摘In order to build method for measuring of elements in honey, the contents of Ba, Cu, Pb, Cd, As, Hg, Cr, Mn, Se had been determined by microwave diges- tion （MWD） and actively coupled plasma atomic emission spectrometry （ICP-AES）. The results show that, the range of heavy metal elements were in the range of 0- 10.0 mg/L, with good linear relationship. In addition, all correlation coefficients kept higher than 0.999, and the recovery rate of ample added with standard solution was in 94.1%-110%. By comparison with the result of chemical experiment, the method was quickly accessible and convenient, widely used in the measurement of elements in honey, with satisfactory results.
基金Supported by Guangzhou Municipal Colleges and Universities with Research Base Project in 2014(14CXY07)Institute of Scientific Research Projects in 2014(LK14002)the Transverse Research Project in 2014(w14001)~~
文摘[Objective] The aim was to establish a rapid method for the determination of the content of total flavonoids in the honey comb. [Method] The content of total flavonoids weas determined based on samples including comb honey, propolis, royal jelly, linden tree honey, with a polyamide column chromatography by spectrophotometry at 360 nm wavelength. [Result] The recovery rate of the method was above 90%, and the variation coefficient was lower than 2%. Compared with the traditional method, this method has less reagent consumption, simple operation and so on.[Conclusion] The content of total flavonoids in comb honey was 14.97 mg/100 g, although is only 0.87% of the total flavone content in propolis. The contained total flavonoids at least doubled than that of others and comb honey is more valuable in the content of flavonoids.
基金Supported by "948" Project of Ministry of Agriculture of China:Study on the Introduction of Agricultural Biological Resources,the Demand of Agricultural Technology and Collection of Policy Information of Africa,Oceania and Other Island Countries(2016-X17)~~
文摘Quality analysis was performed to major cultivars, ‘Hongxin pomelo’ and ‘Wuzi pomelo＇ with uniform maturity. Total soluble solid （TSS）, titratable acid, vita- min C, juice yield, edible rate, single-fruit weight and pesticide residues of the 2 cultivars were detected. The results showed that： the TSS contents of ‘Hongxin pomelo＇ and ‘Wuzi pomelo＇ were in the range of 8%-12%, the vitamin C contents were in the range of 0.3-0.6 g/L, the juice yields were in the range of 56%-68%, the edible rates were in the range of 52%-68%, and the single-fruit weights were in the range of 1-2.5 kg. The titratable acid content was higher in ‘Hongxin pomelo＇ than in ‘Wuzi pomelo＇. Only Dursban and imidacloprid were detected at low con- centrations in pericarp, and the residues of the 3 pesticides were all lower than their detection limits. Therefore, honey pomelo produced in Hainan all could be ate safely.
基金Supported by Youth Entrepreneurship Foundation of Institute of Sericulture and Apiculture,Yunnan Academy of Agricultural Sciences (QC2010002)Special Funds of National Modern Agricultural and bee industry Technology System (CARS-45-SYZ17)Science&Technology Department Project of Yunnan Province (2011BB012)~~
文摘[Objective] The aim was to analyze content of flavones in honey of pomegranate flowers. [Method] Contents of total flavones in 24 honey samples of pomegranate flowers from Mengzi City in 2011 and 2012 were measured as per- spectrophotometry method with acetone as an extractant and rutin as a control. [Result] The average content of flavones in honey of pomegranate flowers was at （2.36±1.36）mg/100 g in 2011 and significantly higher than that in 2012 at （1.71± 0.53）mg/100 g. Analysis was conducted on flavones content in different batches and the results indicated that content of flavones in honey in different batches changed from increasing to decreasing, namely, the content was maximal in peak flowering stage, followed by late flowering stage and initial flowering stage. [Conclusion] The research provides references for development, use and function exploration of the honey of pomegranate flowers.
