CRISPR homing gene drives have considerable potential for managing populations of medically and agriculturally significant insects.They operate by Cas9 cleavage followed by homology-directed repair,copying the drive a...CRISPR homing gene drives have considerable potential for managing populations of medically and agriculturally significant insects.They operate by Cas9 cleavage followed by homology-directed repair,copying the drive allele to the wild-type chromosome and thus increasing in frequency and spreading throughout a population.However,resistance alleles formed by end-joining repair pose a significant obstacle.To address this,we create a homing drive targeting the essential hairy gene in Drosophila melanogaster.Nonfunctional resistance alleles are recessive lethal,while drive carriers have a recoded“rescue”version of hairy.The drive inheritance rate is moderate,and multigenerational cage studies show drive spread to 96%–97%of the population.However,the drive does not reach 100%due to the formation of functional resistance alleles despite using four gRNAs.These alleles have a large deletion but likely utilize an alternate start codon.Thus,revised designs targeting more essential regions of a gene may be necessary to avoid such functional resistance.Replacement of the rescue element’s native 3'UTR with a homolog from another species increases drive inheritance by 13%–24%.This was possibly because of reduced homology between the rescue element and surrounding genomic DNA,which could also be an important design consideration for rescue gene drives.展开更多
Returning home is the most important process of a parallel kinematic machine （PKM） with incremental encoders.Currently,most corresponding articles focus on the accuracy of homing process,and there lacks the investig...Returning home is the most important process of a parallel kinematic machine （PKM） with incremental encoders.Currently,most corresponding articles focus on the accuracy of homing process,and there lacks the investigation of the operation＇s safety.For a 4RRR PKM,all servoaxes would be independently driven to their zero positions at the same time based on the traditional homing mode,and that can bring serious interfere of the kinematic chains.This paper systemically investigates this 4RRR PKM＇s safety of homing process.A homing strategy usually contains three parts which are the home switches＇ locations,the platform＇s initial moving space,and each links＇ homing direction,and all of them can influence the safety of homing operation.For the purpose of evaluating and describing the safety of the homing strategy,some important parameters are introduced as follows：Safely homing ratio （SHR） is used to evaluate the probability of a machine＇s successfully returning home from an initial moving space;Synchronal rotational angle （SRA） is the four links＇ largest synchronal rotational angle with given directions from a given pose.Whether a machine can safely return home from a given pose can be judged by comparing the SRA with all four home switches＇ mounting angles.By meshing the initial moving space and checking the safeties of returning home from all the initial poses on the nodes,the SHR of this initial moving space can be calculate.For the sake of convenience,the platform＇s initial moving space should be as large as possible,and in this 4RRR PKM,a square zone in the center of the workspace with a giving initial rotation range is selected as the platform＇s initial moving space.The forward direction is selected as each link＇s homing direction according to custom,and the platform＇s initial rotational angle is selected as larger than 0° based on this 4RRR PKM＇s kinematic characteristics.The platform＇s initial moving space can be defined only by the side length of the initial moving square.By setting a probable searching step and calculating the SHR of the initial moving square,an optimal procedure of searching for the largest side length of the platform＇s initial moving square is proposed.The homing strategy proposed is based on a systemic research on the safety of homing process for PKM,and the two new indexes SHR and SRA can clearly describe the safety of homing operation.The homing operation based on this strategy is fast and safe,and the method can also be used in other PKMs with the situation of serious components＇ interference.展开更多
The purpose of this paper is to design control loop and guidance loop for the homing missile, to prove that the modified variable structure proportional navigation (VSPN) can be used in passive homing guidance system,...The purpose of this paper is to design control loop and guidance loop for the homing missile, to prove that the modified variable structure proportional navigation (VSPN) can be used in passive homing guidance system, and to compare the VSPN with the proportional navigation (PN). Angular rate and linear acceleration are fed back to improve the stability and robust of autopilot, VSPN and PN are compared in mathematics simulation of homing missile in attacking tanks and helicopters. The damp and the stability of the missile body are increased. The VSPN cuts down the overload of guidance phase dramatically, makes the ballistic trajectory straighter than PN and leads to smaller missdistance. The VSPN can be used for passive homing guidance system, and is especially suitable for attacking helicopter.展开更多
Mesenchymal stromal cells(MSCs) are currently being investigated for use in a wide variety of clinical applications. For most of these applications, systemic delivery of the cells is preferred. However, this requires ...Mesenchymal stromal cells(MSCs) are currently being investigated for use in a wide variety of clinical applications. For most of these applications, systemic delivery of the cells is preferred. However, this requires the homing and migration of MSCs to a target tissue. Although MSC hominghas been described, this process does not appear to be highly efficacious because only a few cells reach the target tissue and remain there after systemic administration. This has been ascribed to low expression levels of homing molecules, the loss of expression of such molecules during expansion, and the heterogeneity of MSCs in cultures and MSC culture protocols. To overcome these limitations, different methods to improve the homing capacity of MSCs have been examined. Here, we review the current understanding of MSC homing, with a particular focus on homing to bone marrow. In addition, we summarize the strategies that have been developed to improve this process. A better understanding of MSC biology, MSC migration and homing mechanisms will allow us to prepare MSCs with optimal homing capacities. The efficacy of therapeutic applications is dependent on efficient delivery of the cells and can, therefore, only benefit from better insights into the homing mechanisms.展开更多
Objective The macula lagena in birds is located at the apical end of the cochlea and contains many tiny otoliths. The macula lagena is innervated and has neural projections to the brainstem, but its physiological func...Objective The macula lagena in birds is located at the apical end of the cochlea and contains many tiny otoliths. The macula lagena is innervated and has neural projections to the brainstem, but its physiological function is still unclear. It remains disputable that it is because otoliths in the lagena are rich in elements Fe and Zn that birds can obtain geomagnetic information for homing. To clarify this issue, we carried out a study to determine whether or not otoliths in the lagena of homing pigeons are richer in magnetic elements than those in the saccule and the utricle. Methods The contents of ferromagnetic elements （Fe, Co, Ni） and other metal elements in lagenal otoliths of adult homing pigeons were precisely analyzed with inductively coupled plasma mass spectrometry （ICP-MS） of high sensitivity, and then they were compared with those in saccular and utricular otoliths （all the contents were normalized to Ca）. Results In adult homing pigeons, the contents of ferromagnetic elements （Fe, Co, Ni） in lagenal otoliths were less than 0.7% （normalized to Ca element） and were the same order in magnitude as those in saccular and utricular otoliths. The content of Fe in lagenal otoliths was not significantly different from that in utricular otoliths and was even lower than that in saccular otoliths. The content of Co in lagenal otoliths was lower than that in saccular otoliths and higher than that in utricular otoliths. The content of Ni in lagenal otoliths was not significantly different from that in saccular otoliths and was higher than that in utricular otoliths. The contents of other metal elements Na, Mg, K, Al, Mn and Pb in lagenal otoliths were not significantly different from those in utricular and saccular otoliths. The contents of metal elements Zn, Ba and Cu in lagenal otoliths were lower than those in saccular otoliths. Conclusion The contents of magnetic elements in lagenal otoliths of homing pigeons are not much higher than those in utricular and saccular otoliths, which does not support the hypothesis that birds depend on high contents of Fe and Zn in lagenal otoliths for sensation of geomagnetic information. Similarities in morphology, element ingredient and element content between lagenal otoliths and utricular otoliths suggest that the two types of otolithic organs may play similar roles in sensing gravitational and acceleration signals.展开更多
The objective is to design a variable structure proportional navigation law for the passive homingmissile so that the missile can hit the target with a minimum miss-distance and desired impact attitude anglewithin the...The objective is to design a variable structure proportional navigation law for the passive homingmissile so that the missile can hit the target with a minimum miss-distance and desired impact attitude anglewithin the required overlcad when it flies at a lower trajectory.This paper utilized the variable structure propor-tional navigation with terminal impact attitude angular constraint,and made estimation about the distance rateand distance between the missile and target,which broke the limitation of a passive homing seeker.Furthermore,the paper let the switch gain vary adaptively with time to avoid any larger initial overload.The mathematicalsimulation was completed by some studied passive homing missile's dynamic model,and the simulation resultsshowed that the navigation law of the passive homing missile can make the missile get the required miss-distance and desired impact attitude angle against tanks at lower trajectories and within the maximum overload;also this guidance law is much robust to the estimation eror of distance and distance rate.展开更多
Objective:To investigate the effects of panax notoginseng saponins(PNS) on homing of C-kit+ bone mesenchymal stem cells(BMSCs) to the infarction heart.Methods:The acute myocardial infraction(AMI) model was established...Objective:To investigate the effects of panax notoginseng saponins(PNS) on homing of C-kit+ bone mesenchymal stem cells(BMSCs) to the infarction heart.Methods:The acute myocardial infraction(AMI) model was established in 140 Wistar rats,105 model rats survived after operation,and the model rats were randomly divided into five groups,21 rats in each group:Western medicine group mobilized by subcutaneous injection of human granuloctye colony stimulating factor(G-CSF) 50 μg·kg-1·d-1;sham operation group and a model group treated by subcutaneous injection of normal saline 50 μg·kg-1·d-1;Chinese medicine group mobilized by intraperitoneal injection of Xuesaitong(血塞通)(ingredients of PNS) 150 mg·kg-1·d-1;integrative medicine group mobilized by subcutaneous injection of G-CSF 50 μg·kg-1·d-1 and intraperitoneal injection of Xuesaitong 150 mg·kg-1·d-1.Except for the sham-operated group,each group was divided into three sub-groups by three time points of 1 d,7 d and 14 d.G-CSF was injected once a day for 7 d.Xuesaitong was injected once a day until the rats were killed.The flow cytometry was used for detection of C-kit + cells in the peripheral blood in different time points,and immunohistochemical method was used for detection of the changes of C-kit + cell and Ki-67+ cell numbers in the marginal zone of AMI.Results:Twenty-four hours after the operation,C-kit + cells had a slight increase in the model group compared with the sham operation group(P>0.05).The peripheral blood C-kit+ cells in the integrative group increased significantly compared with the other groups on 7 d and 14 d(all P<0.05).Meanwhile the expression of C-kit + cells and Ki-67+ cells in the marginal zone of AMI in the integrative group increased significantly compared with the Chinese medicine group,the western medicine group and the model group on 1 d,7 d and 14 d(all P<0.05),and the cells in the integrative group decreased significantly on 14 d compared with that on 7 d(P<0.05).Conclusion:PNS can cooperate with G-CSF to mobilize C-kit+ BMSCs from the marrow into the peripheral blood and promote them "homing" to the infarction heart.展开更多
TMTP1, a 5-amino acid peptide NVVRQ, obtained by using the flagella peptide library screening in our previous studies, can be used for the labeling of malignant in situ and metastatic lesions, and even micro-metastase...TMTP1, a 5-amino acid peptide NVVRQ, obtained by using the flagella peptide library screening in our previous studies, can be used for the labeling of malignant in situ and metastatic lesions, and even micro-metastases. In this study, TMTP1 was assessed for its ability to specifically target the malignant hematopoietic cells and metastatic lesions of hematological malignancies. FITC-TMTP1 was chemically synthesized. Immunofluorescence assay and competitive test were carried out to determine the specific binding capacity of TMTPl to hematological malignant cell lines, including HL60, k562, SHI-1, Jurkat, Raji, El-4 and umbilical cord blood mononuclear cells. Mononuclear cells were isolated from the bone marrow of healthy subjects and patients with chronic myeloid leukemia. Then the cells were co-clutured with TMTP1 or scrambled peptides and the binding and affinity of TMTP1 peptide to the primary cells of hematological malignancies were flow cytometrically analyzed. The binding speci-ficity of TMTP1 to target hematological malignancies was measured in vivo by intravenous injection of FITC-conjugated TMTP1 into El-4 lymphoma-bearing mice. The results showed that TMTP1 specifi-cally bound to the cells of a series of hematological malignancies, including HL60, k562, Jurkat, Raji , El-4 and chronic myeloid leukemia primary cells but not to bone marrow mononuclear cells from healthy subjects. By contrast, TMTP1 could bind to the metastatic foci of lymphoma originating from the EL-4 cell line while the scrambled peptide failed to do so. Moreover, the occult metastases could be identified, with high specificity, by detecting FITC-TMTP1. We are led to conclude that TMTP1, as a novel tumor-homing peptide, can serve as a marker for primary malignant and metastatic lesions for the early diagnosis of hematological malignances and a carrier of anticancer drugs for cancer treatment.展开更多
This paper focuses mainly on semi-strapdown image homing guided （SSIHG） system design based on optical flow for a six-degree-of-freedom （6-DOF） axial-symmetric skid-to-turn missile. Three optical flow algorithms s...This paper focuses mainly on semi-strapdown image homing guided （SSIHG） system design based on optical flow for a six-degree-of-freedom （6-DOF） axial-symmetric skid-to-turn missile. Three optical flow algorithms suitable for large displacements are introduced and compared. The influence of different displacements on computational accuracy of the three algorithms is analyzed statistically. The total optical flow of the SSIHG missile is obtained using the Scale Invariant Feature Transform （SIFT） algorithm, which is the best among the three for large displacements. After removing the rotational optical flow caused by rotation of the gimbal and missile body from the total optical flow, the remaining translational optical flow is smoothed via Kalman filtering. The circular navigation guidance （CNG） law with impact angle constraint is then obtained utilizing the smoothed translational optical flow and position of the target image. Simulations are carried out under both disturbed and undisturbed conditions, and results indicate the proposed guidance strategy for SSIHG missiles can result in a precise target hit with a desired impact angle without the need for the time-to-go parameter.展开更多
Traditional parafoil homing usually uses a point as object. As the mobility of parafoil is limited by its glide ratio and wind, in some cases when the parafoil scatter area is large, or the glide ratio of parafoil is ...Traditional parafoil homing usually uses a point as object. As the mobility of parafoil is limited by its glide ratio and wind, in some cases when the parafoil scatter area is large, or the glide ratio of parafoil is small, the deviation of its landing point to object point will be arduous to control. Accordingly, during these situations, when parafoil is used in recovery of spacecraft or satellite, the landing area of parafoil can be set as a rectangle, and the object of parafoil can be set as a line segment. The thesis of this work is designing an algorithm for parafoil homing using line segment as object. The algorithm of wind velocity and direction calculation in different flying segments was also investigated. The algorithm designed navigates the parafoil to land into the predestined area and largely reduce the probability of recovery loads falling to unwanted area to damage houses and people.展开更多
This study investigated the 'homing' phenomenon in hepatocellular carcinoma (HCC).The 'homing' specificity of endothelial progenitor cells (EPC) by establishing an orthotopic implantation model in nude...This study investigated the 'homing' phenomenon in hepatocellular carcinoma (HCC).The 'homing' specificity of endothelial progenitor cells (EPC) by establishing an orthotopic implantation model in nude mice.EPCs harvested from the marrow cells were separated by density gradient centrifugation.Fluorescence microscope,flow cytometry (FCM) and double fluorescence staining with FITC-UEA-I and DiI-ac-LDL,were employed to identify the cells.4',6-diamidino-2-phenylindole (DAPI) labelling and real-time PCR were used for detecting the expression of CD133 and chemokines to trace and observe the distribution of EPCs.Our results showed that the distribution rate of EPCs was obviously higher than that in other important organs and the negative control group.Detection of CD133 and chemokines yielded similar results in difference tissues.Our experiment confirmed that the chemotaxis of EPCs does exist in HCC.Moreover,HIF-1α,SDF-1 and VEGF might play important roles in the 'homing' of EPCs in HCC.EPCs might be a potential candidate for targeting vector of HCC for gene therapy.展开更多
This paper comprehensively analyzes the attitude pursuit guidance law whichis one of the simple guidance methods for strapdown homing guidance system. Via the analyticalsolution of attitude pursuit, limitations on the...This paper comprehensively analyzes the attitude pursuit guidance law whichis one of the simple guidance methods for strapdown homing guidance system. Via the analyticalsolution of attitude pursuit, limitations on the velocities of missile and target are presented. Theinfluence of damping, lagging and transfer parameter from rudder deviation to angle of attack onthe attitude pursuit guidance law is discussed by using Automatic Control Theory. It is concludedthat the velocities of missile and target should not be too large, and the stable loop of missilebody should have some damping when the missile guidance system has inherent lagging, and thetransfer parameter mentioned above should not be too large when the attitude pursuit guidance law isused.展开更多
In order to realize safe and accurate homing of parafoil system,a multiphase homing trajectory planning scheme is proposed according to the maneuverability and basic flight characteristics of the vehicle.In this scena...In order to realize safe and accurate homing of parafoil system,a multiphase homing trajectory planning scheme is proposed according to the maneuverability and basic flight characteristics of the vehicle.In this scenario,on the basis of geometric relationship of each phase trajectory,the problem of trajectory planning is transformed to parameter optimizing,and then auxiliary population-based quantum differential evolution algorithm(AP-QDEA)is applied as a tool to optimize the objective function,and the design parameters of the whole homing trajectory are obtained.The proposed AP-QDEA combines the strengths of differential evolution algorithm(DEA)and quantum evolution algorithm(QEA),and the notion of auxiliary population is introduced into the proposed algorithm to improve the searching precision and speed.The simulation results show that the proposed AP-QDEA is proven its superior in both effectiveness and efficiency by solving a set of benchmark problems,and the multiphase homing scheme can fulfill the requirement of fixed-points and upwind landing in the process of homing which is simple in control and facile in practice as well.展开更多
A smart homing guidance strategy with control saturation against a target-defender team is derived. It is noteworthy that a cooperative strategy of the target-defender team is applied,which has been proved more challe...A smart homing guidance strategy with control saturation against a target-defender team is derived. It is noteworthy that a cooperative strategy of the target-defender team is applied,which has been proved more challenging for the homing guidance.The defender missile is launched by the target and guided by a cooperative augmented proportional navigation(APN). At the same time, the target performs a one-switch maneuver to cooperate and minimize the defender's acceleration requirement. The problem is analyzed for arbitrary-order linear dynamics of the agents in the linearized form but validated by the mathematical simulations by using nonlinear kinematics. The perfect information of three agents' states is assumed. Then, a method to deal with the target-defender team is proposed. It contains a combined performance index penalizing the miss distance relative to the target and energy consumption in the whole duration. Besides, the specific miss distance related to the defender is regarded as an inequality constraint. An analytical solution for the smart guidance strategy against the APN guided defender is derived. Meanwhile, the control saturations are introduced to get more realistic and reasonable insights to this practical target-missile-defender problem. A simple but effective iterative searching technique is proposed to determine the saturation time points. The solution provides an optimal homing strategy to evade the defender with a specific miss distance and intercept the target with the minimum miss distance in the minimum energy manner. Nonlinear two-dimensional simulation results are used to validate the theoretical analysis. By comparison with the optimal differential game guidance(ODGG) and the combined minimum effort guidance(CMEG), the superiority of this smart guidance strategy is concluded.展开更多
In this paper,a new homing guidance method is used to control the flying time and falling angle for guided missiles. Through this approach,it finds the approximate solution to the quadratic equation of time-togo,which...In this paper,a new homing guidance method is used to control the flying time and falling angle for guided missiles. Through this approach,it finds the approximate solution to the quadratic equation of time-togo,which is used for the formula derivation of the flying time control command. In this guidance law design,the acceleration rate control command is adopted. The guidance law is composed of a PN guidance command and a flying time control command. Firstly,it obtains a desired falling angle with accurate guidance. Secondly,it introduces to satisfy the constraint of flying time. The flying time control requires an assumption on the future evolution of missile,which is called time-to-go. To cope with the time-varying speed of missiles,a method of compensating the estimation of time-to-go is presented. The new guidance law is evaluated by using a simulation of typical terminal guidance for rocket-propelled torpedo. The simulation results show that the guidance achieves excellent control performance and exhibits insensitivity to initial trajectory parameter over a widen flight envelope.展开更多
BACKGROUND Stroke is the second leading cause of death worldwide.There is a real need to develop treatment strategies for reducing neurological deficits in stroke survivors,and stem cell(SC)therapeutics appear to be a...BACKGROUND Stroke is the second leading cause of death worldwide.There is a real need to develop treatment strategies for reducing neurological deficits in stroke survivors,and stem cell(SC)therapeutics appear to be a promising alternative for stroke therapy that can be used in combination with approved thrombolytic or thrombectomy approaches.However,the efficacy of SC therapy depends on the SC homing ability and engraftment into the injury site over a long period of time.Nonetheless,tracking SCs from their niche to the target tissues is a complex process.AIM To evaluate SC migration homing,tracking and therapeutic efficacy in the treatment of stroke using nanoparticles METHODS A systematic literature search was performed to identify articles published prior to November 2019 that were indexed in PubMed and Scopus.The following inclusion criteria were used:(1)Studies that used in vivo models of stroke or ischemic brain lesions;(2)Studies of SCs labeled with some type of contrast agent for cell migration detection;and(3)Studies that involved in vivo cellular homing and tracking analysis.RESULTS A total of 82 articles were identified by indexing in Scopus and Pub Med.Afterthe inclusion criteria were applied,35 studies were selected,and the articles were assessed for eligibility;ultimately,only 25 studies were included.Most of the selected studies used SCs from human and mouse bone marrow labeled with magnetic nanoparticles alone or combined with fluorophore dyes.These cells were administered in the stroke model(to treat middle cerebral artery occlusion in 74%of studies and for photothrombotic induction in 26%of studies).Fiftythree percent of studies used xenogeneic grafts for cell therapy,and the migration homing and tracking evaluation was performed by magnetic resonance imaging as well as other techniques,such as near-infrared fluorescence imaging(12%)or bioluminescence assays(12%).CONCLUSION Our systematic review provided an up-to-date evaluation of SC migration homing and the efficacy of cellular therapy for stroke treatment in terms of functional and structural improvements in the late stage.展开更多
Homing trajectory planning is a core task of autonomous homing of parafoil system.This work analyzes and establishes a simplified kinematic mathematical model,and regards the homing trajectory planning problem as a ki...Homing trajectory planning is a core task of autonomous homing of parafoil system.This work analyzes and establishes a simplified kinematic mathematical model,and regards the homing trajectory planning problem as a kind of multi-objective optimization problem.Being different from traditional ways of transforming the multi-objective optimization into a single objective optimization by weighting factors,this work applies an improved non-dominated sorting genetic algorithm Ⅱ(NSGA Ⅱ) to solve it directly by means of optimizing multi-objective functions simultaneously.In the improved NSGA Ⅱ,the chaos initialization and a crowding distance based population trimming method were introduced to overcome the prematurity of population,the penalty function was used in handling constraints,and the optimal solution was selected according to the method of fuzzy set theory.Simulation results of three different schemes designed according to various practical engineering requirements show that the improved NSGA Ⅱ can effectively obtain the Pareto optimal solution set under different weighting with outstanding convergence and stability,and provide a new train of thoughts to design homing trajectory of parafoil system.展开更多
The strapdown homing guidance system for some ammunition was mainly studied. A strong tracking Kalman filter was designed for the strapdown homing guidance system using the information measured by the strapdown homing...The strapdown homing guidance system for some ammunition was mainly studied. A strong tracking Kalman filter was designed for the strapdown homing guidance system using the information measured by the strapdown homing seeker to estimate relative movement variables between the ammunition and target. Then the optimal proportional law, which using the estimated information, guided the ammunition. Simulation results show that the designed strapdown homing guidance system with strong tracking Kalman filter can attack the maneuvering target effectively, and satisfy the performance index for the guided ammunition system.展开更多
OBJECTIVE: To investigate the effects of Ermiao Fang(EM) with medical guide Xixin(Herba Asari Mandshurici)(HAM) on bone marrow stem cell migration to a focal zone in osteoarthritis(OA) rats.METHODS: OA rats were induc...OBJECTIVE: To investigate the effects of Ermiao Fang(EM) with medical guide Xixin(Herba Asari Mandshurici)(HAM) on bone marrow stem cell migration to a focal zone in osteoarthritis(OA) rats.METHODS: OA rats were induced by arthrectomy and assigned to sham-operated, model, EM, or EM plus HAM groups.All rats were injected with recombinant human granulocyte colony-stimulating factor 30μg·kg-1·d-1for7 days and treated with EMor EM plus HAM at 1.6 or 1.9 g·kg-1·d-1 for 3 or 6 weeks, respectively. Chondrocyte apoptosis and cartilage matrix components were tested by transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assay and special staining. Levels of interleukin-1 beta(IL-1β) tumor necrosis factor alpha(TNF-α) nitric oxide(NO), and inducible nitric oxide synthase(iNOS) in serum were detected by enzyme-linked immunosorbent assay or radioimmunoassay. Matrix metalloproteinases(MMPs)-13,tissue inhibitors of metalloproteinases(TIMPs)-1,Bromodeoxyuridine(BrdU), cluster of differentiation 34(CD34), and stromal cell-derived factor 1(SDF-1) were measured by immunohistochemical assay.RESULTS:The EM and EM plus HAM groups had significantly less cartilage damage and synovium inflammation the model group. Moreover, the EM and EM plus HAM groups had less chondrocyte apoptosis and more proteoglycan and collagen content than the model group.The EM and EMplus HAM groups had obviously higher MMPs-13 and TIMPs-1 expression in the cartilage than the model group. Moreover, the two formula groups had less release of IL-1β, TNF-α, NO, and iNOS than model group. Importantly, the expressions of BrdU, CD34,and SDF-1 in cartilage were significantly higher in the EM and EM plus HAM-Medtreated rats than model group. Notably, the EM plus HAM treatment seemed to have the greatest effects.CONCLUSION: HAM improves the therapeutic effects of EM on OA rats by enhancing BMSC directional homing to the focal zone.展开更多
Background:Efficient and safe movement is fundamental for wild birds to thrive in their environments.For arboreal forest animals,especially birds,canopy cover has a large impact on birds’daily movements and is a cruc...Background:Efficient and safe movement is fundamental for wild birds to thrive in their environments.For arboreal forest animals,especially birds,canopy cover has a large impact on birds’daily movements and is a crucial component of conservation strategies seeking to retain avian population in disturbed or urban habitats.Methods:We translocated woodland bird species utilizing different forest strata during two non-breeding seasons in Gainesville,FL,USA.We used linear model and generalized linear model to examine the effects of canopy cover and species identity on homing success and speed.Results:Among our study species of Tufted Titmouse(Baeolophus bicolor),Carolina Chickadee(Poecile carolinensis),and Northern Cardinal(Cardinalis cardinalis),we found that Carolina Chickadees and Tufted Titmice were more likely to return than Northern Cardinals.Among birds that successfully returned,homing speed is significantly affected by forest canopy cover and species identity(titmice had higher homing speed than cardinals).Birds return much faster in landscape with higher canopy cover.Conclusions:This study presented evidence of species identity’s effect on homing success and speed in common feeder bird species in Southeast US and provided further evidence that bird movements in the suburban land cover are constrained by low canopy cover.展开更多
基金supported by laboratory startup funds from Peking University and the Center for Life Sciences,as well as the grants from the National Science Foundation of China(32302455 and 32270672)。
文摘CRISPR homing gene drives have considerable potential for managing populations of medically and agriculturally significant insects.They operate by Cas9 cleavage followed by homology-directed repair,copying the drive allele to the wild-type chromosome and thus increasing in frequency and spreading throughout a population.However,resistance alleles formed by end-joining repair pose a significant obstacle.To address this,we create a homing drive targeting the essential hairy gene in Drosophila melanogaster.Nonfunctional resistance alleles are recessive lethal,while drive carriers have a recoded“rescue”version of hairy.The drive inheritance rate is moderate,and multigenerational cage studies show drive spread to 96%–97%of the population.However,the drive does not reach 100%due to the formation of functional resistance alleles despite using four gRNAs.These alleles have a large deletion but likely utilize an alternate start codon.Thus,revised designs targeting more essential regions of a gene may be necessary to avoid such functional resistance.Replacement of the rescue element’s native 3'UTR with a homolog from another species increases drive inheritance by 13%–24%.This was possibly because of reduced homology between the rescue element and surrounding genomic DNA,which could also be an important design consideration for rescue gene drives.
基金supported by National Natural Science Foundation of China (Grant No. 50775125,and No. 50775117)National Hi-tech Research and Development Program of China (863 Program,Grant No.2007AA041901)National Basic Research Program of China (973 Program,Grant No. 2004CB318007)
文摘Returning home is the most important process of a parallel kinematic machine （PKM） with incremental encoders.Currently,most corresponding articles focus on the accuracy of homing process,and there lacks the investigation of the operation＇s safety.For a 4RRR PKM,all servoaxes would be independently driven to their zero positions at the same time based on the traditional homing mode,and that can bring serious interfere of the kinematic chains.This paper systemically investigates this 4RRR PKM＇s safety of homing process.A homing strategy usually contains three parts which are the home switches＇ locations,the platform＇s initial moving space,and each links＇ homing direction,and all of them can influence the safety of homing operation.For the purpose of evaluating and describing the safety of the homing strategy,some important parameters are introduced as follows：Safely homing ratio （SHR） is used to evaluate the probability of a machine＇s successfully returning home from an initial moving space;Synchronal rotational angle （SRA） is the four links＇ largest synchronal rotational angle with given directions from a given pose.Whether a machine can safely return home from a given pose can be judged by comparing the SRA with all four home switches＇ mounting angles.By meshing the initial moving space and checking the safeties of returning home from all the initial poses on the nodes,the SHR of this initial moving space can be calculate.For the sake of convenience,the platform＇s initial moving space should be as large as possible,and in this 4RRR PKM,a square zone in the center of the workspace with a giving initial rotation range is selected as the platform＇s initial moving space.The forward direction is selected as each link＇s homing direction according to custom,and the platform＇s initial rotational angle is selected as larger than 0° based on this 4RRR PKM＇s kinematic characteristics.The platform＇s initial moving space can be defined only by the side length of the initial moving square.By setting a probable searching step and calculating the SHR of the initial moving square,an optimal procedure of searching for the largest side length of the platform＇s initial moving square is proposed.The homing strategy proposed is based on a systemic research on the safety of homing process for PKM,and the two new indexes SHR and SRA can clearly describe the safety of homing operation.The homing operation based on this strategy is fast and safe,and the method can also be used in other PKMs with the situation of serious components＇ interference.
文摘The purpose of this paper is to design control loop and guidance loop for the homing missile, to prove that the modified variable structure proportional navigation (VSPN) can be used in passive homing guidance system, and to compare the VSPN with the proportional navigation (PN). Angular rate and linear acceleration are fed back to improve the stability and robust of autopilot, VSPN and PN are compared in mathematics simulation of homing missile in attacking tanks and helicopters. The damp and the stability of the missile body are increased. The VSPN cuts down the overload of guidance phase dramatically, makes the ballistic trajectory straighter than PN and leads to smaller missdistance. The VSPN can be used for passive homing guidance system, and is especially suitable for attacking helicopter.
文摘Mesenchymal stromal cells(MSCs) are currently being investigated for use in a wide variety of clinical applications. For most of these applications, systemic delivery of the cells is preferred. However, this requires the homing and migration of MSCs to a target tissue. Although MSC hominghas been described, this process does not appear to be highly efficacious because only a few cells reach the target tissue and remain there after systemic administration. This has been ascribed to low expression levels of homing molecules, the loss of expression of such molecules during expansion, and the heterogeneity of MSCs in cultures and MSC culture protocols. To overcome these limitations, different methods to improve the homing capacity of MSCs have been examined. Here, we review the current understanding of MSC homing, with a particular focus on homing to bone marrow. In addition, we summarize the strategies that have been developed to improve this process. A better understanding of MSC biology, MSC migration and homing mechanisms will allow us to prepare MSCs with optimal homing capacities. The efficacy of therapeutic applications is dependent on efficient delivery of the cells and can, therefore, only benefit from better insights into the homing mechanisms.
基金supported by the National Natural Science Foundation of China(No.30470560 and 30730041)the National Basic Research Program of China (No.2007CB512306)the Knowledge Innovation Project of Chinese Academy of Sciences (No.KSCX1-YW-R-36)
文摘Objective The macula lagena in birds is located at the apical end of the cochlea and contains many tiny otoliths. The macula lagena is innervated and has neural projections to the brainstem, but its physiological function is still unclear. It remains disputable that it is because otoliths in the lagena are rich in elements Fe and Zn that birds can obtain geomagnetic information for homing. To clarify this issue, we carried out a study to determine whether or not otoliths in the lagena of homing pigeons are richer in magnetic elements than those in the saccule and the utricle. Methods The contents of ferromagnetic elements （Fe, Co, Ni） and other metal elements in lagenal otoliths of adult homing pigeons were precisely analyzed with inductively coupled plasma mass spectrometry （ICP-MS） of high sensitivity, and then they were compared with those in saccular and utricular otoliths （all the contents were normalized to Ca）. Results In adult homing pigeons, the contents of ferromagnetic elements （Fe, Co, Ni） in lagenal otoliths were less than 0.7% （normalized to Ca element） and were the same order in magnitude as those in saccular and utricular otoliths. The content of Fe in lagenal otoliths was not significantly different from that in utricular otoliths and was even lower than that in saccular otoliths. The content of Co in lagenal otoliths was lower than that in saccular otoliths and higher than that in utricular otoliths. The content of Ni in lagenal otoliths was not significantly different from that in saccular otoliths and was higher than that in utricular otoliths. The contents of other metal elements Na, Mg, K, Al, Mn and Pb in lagenal otoliths were not significantly different from those in utricular and saccular otoliths. The contents of metal elements Zn, Ba and Cu in lagenal otoliths were lower than those in saccular otoliths. Conclusion The contents of magnetic elements in lagenal otoliths of homing pigeons are not much higher than those in utricular and saccular otoliths, which does not support the hypothesis that birds depend on high contents of Fe and Zn in lagenal otoliths for sensation of geomagnetic information. Similarities in morphology, element ingredient and element content between lagenal otoliths and utricular otoliths suggest that the two types of otolithic organs may play similar roles in sensing gravitational and acceleration signals.
文摘The objective is to design a variable structure proportional navigation law for the passive homingmissile so that the missile can hit the target with a minimum miss-distance and desired impact attitude anglewithin the required overlcad when it flies at a lower trajectory.This paper utilized the variable structure propor-tional navigation with terminal impact attitude angular constraint,and made estimation about the distance rateand distance between the missile and target,which broke the limitation of a passive homing seeker.Furthermore,the paper let the switch gain vary adaptively with time to avoid any larger initial overload.The mathematicalsimulation was completed by some studied passive homing missile's dynamic model,and the simulation resultsshowed that the navigation law of the passive homing missile can make the missile get the required miss-distance and desired impact attitude angle against tanks at lower trajectories and within the maximum overload;also this guidance law is much robust to the estimation eror of distance and distance rate.
基金supported by Important National Basic Research Program of China (973 Program, N0.2003CB517103)China Postdoctoral Foundation (No. 20070410129)
文摘Objective:To investigate the effects of panax notoginseng saponins(PNS) on homing of C-kit+ bone mesenchymal stem cells(BMSCs) to the infarction heart.Methods:The acute myocardial infraction(AMI) model was established in 140 Wistar rats,105 model rats survived after operation,and the model rats were randomly divided into five groups,21 rats in each group:Western medicine group mobilized by subcutaneous injection of human granuloctye colony stimulating factor(G-CSF) 50 μg·kg-1·d-1;sham operation group and a model group treated by subcutaneous injection of normal saline 50 μg·kg-1·d-1;Chinese medicine group mobilized by intraperitoneal injection of Xuesaitong(血塞通)(ingredients of PNS) 150 mg·kg-1·d-1;integrative medicine group mobilized by subcutaneous injection of G-CSF 50 μg·kg-1·d-1 and intraperitoneal injection of Xuesaitong 150 mg·kg-1·d-1.Except for the sham-operated group,each group was divided into three sub-groups by three time points of 1 d,7 d and 14 d.G-CSF was injected once a day for 7 d.Xuesaitong was injected once a day until the rats were killed.The flow cytometry was used for detection of C-kit + cells in the peripheral blood in different time points,and immunohistochemical method was used for detection of the changes of C-kit + cell and Ki-67+ cell numbers in the marginal zone of AMI.Results:Twenty-four hours after the operation,C-kit + cells had a slight increase in the model group compared with the sham operation group(P>0.05).The peripheral blood C-kit+ cells in the integrative group increased significantly compared with the other groups on 7 d and 14 d(all P<0.05).Meanwhile the expression of C-kit + cells and Ki-67+ cells in the marginal zone of AMI in the integrative group increased significantly compared with the Chinese medicine group,the western medicine group and the model group on 1 d,7 d and 14 d(all P<0.05),and the cells in the integrative group decreased significantly on 14 d compared with that on 7 d(P<0.05).Conclusion:PNS can cooperate with G-CSF to mobilize C-kit+ BMSCs from the marrow into the peripheral blood and promote them "homing" to the infarction heart.
基金supported by the National Science Foundation of China (No. 30800402)
文摘TMTP1, a 5-amino acid peptide NVVRQ, obtained by using the flagella peptide library screening in our previous studies, can be used for the labeling of malignant in situ and metastatic lesions, and even micro-metastases. In this study, TMTP1 was assessed for its ability to specifically target the malignant hematopoietic cells and metastatic lesions of hematological malignancies. FITC-TMTP1 was chemically synthesized. Immunofluorescence assay and competitive test were carried out to determine the specific binding capacity of TMTPl to hematological malignant cell lines, including HL60, k562, SHI-1, Jurkat, Raji, El-4 and umbilical cord blood mononuclear cells. Mononuclear cells were isolated from the bone marrow of healthy subjects and patients with chronic myeloid leukemia. Then the cells were co-clutured with TMTP1 or scrambled peptides and the binding and affinity of TMTP1 peptide to the primary cells of hematological malignancies were flow cytometrically analyzed. The binding speci-ficity of TMTP1 to target hematological malignancies was measured in vivo by intravenous injection of FITC-conjugated TMTP1 into El-4 lymphoma-bearing mice. The results showed that TMTP1 specifi-cally bound to the cells of a series of hematological malignancies, including HL60, k562, Jurkat, Raji , El-4 and chronic myeloid leukemia primary cells but not to bone marrow mononuclear cells from healthy subjects. By contrast, TMTP1 could bind to the metastatic foci of lymphoma originating from the EL-4 cell line while the scrambled peptide failed to do so. Moreover, the occult metastases could be identified, with high specificity, by detecting FITC-TMTP1. We are led to conclude that TMTP1, as a novel tumor-homing peptide, can serve as a marker for primary malignant and metastatic lesions for the early diagnosis of hematological malignances and a carrier of anticancer drugs for cancer treatment.
基金supported by the Armament Research Fund of China (No.9020A02010313BQ01)
文摘This paper focuses mainly on semi-strapdown image homing guided （SSIHG） system design based on optical flow for a six-degree-of-freedom （6-DOF） axial-symmetric skid-to-turn missile. Three optical flow algorithms suitable for large displacements are introduced and compared. The influence of different displacements on computational accuracy of the three algorithms is analyzed statistically. The total optical flow of the SSIHG missile is obtained using the Scale Invariant Feature Transform （SIFT） algorithm, which is the best among the three for large displacements. After removing the rotational optical flow caused by rotation of the gimbal and missile body from the total optical flow, the remaining translational optical flow is smoothed via Kalman filtering. The circular navigation guidance （CNG） law with impact angle constraint is then obtained utilizing the smoothed translational optical flow and position of the target image. Simulations are carried out under both disturbed and undisturbed conditions, and results indicate the proposed guidance strategy for SSIHG missiles can result in a precise target hit with a desired impact angle without the need for the time-to-go parameter.
基金Project(61503077)supported by the National Natural Science Foundation of ChinaProject(BK20130628)supported by the Jiangsu Natural Science Foundation,China
文摘Traditional parafoil homing usually uses a point as object. As the mobility of parafoil is limited by its glide ratio and wind, in some cases when the parafoil scatter area is large, or the glide ratio of parafoil is small, the deviation of its landing point to object point will be arduous to control. Accordingly, during these situations, when parafoil is used in recovery of spacecraft or satellite, the landing area of parafoil can be set as a rectangle, and the object of parafoil can be set as a line segment. The thesis of this work is designing an algorithm for parafoil homing using line segment as object. The algorithm of wind velocity and direction calculation in different flying segments was also investigated. The algorithm designed navigates the parafoil to land into the predestined area and largely reduce the probability of recovery loads falling to unwanted area to damage houses and people.
基金supported by a grant from the Natural Science Foundation of Zhejiang Province (No.Y2090538)a grant from Wenzhou Municipal Science and Technology Foundation (No.Y20090017)
文摘This study investigated the 'homing' phenomenon in hepatocellular carcinoma (HCC).The 'homing' specificity of endothelial progenitor cells (EPC) by establishing an orthotopic implantation model in nude mice.EPCs harvested from the marrow cells were separated by density gradient centrifugation.Fluorescence microscope,flow cytometry (FCM) and double fluorescence staining with FITC-UEA-I and DiI-ac-LDL,were employed to identify the cells.4',6-diamidino-2-phenylindole (DAPI) labelling and real-time PCR were used for detecting the expression of CD133 and chemokines to trace and observe the distribution of EPCs.Our results showed that the distribution rate of EPCs was obviously higher than that in other important organs and the negative control group.Detection of CD133 and chemokines yielded similar results in difference tissues.Our experiment confirmed that the chemotaxis of EPCs does exist in HCC.Moreover,HIF-1α,SDF-1 and VEGF might play important roles in the 'homing' of EPCs in HCC.EPCs might be a potential candidate for targeting vector of HCC for gene therapy.
文摘This paper comprehensively analyzes the attitude pursuit guidance law whichis one of the simple guidance methods for strapdown homing guidance system. Via the analyticalsolution of attitude pursuit, limitations on the velocities of missile and target are presented. Theinfluence of damping, lagging and transfer parameter from rudder deviation to angle of attack onthe attitude pursuit guidance law is discussed by using Automatic Control Theory. It is concludedthat the velocities of missile and target should not be too large, and the stable loop of missilebody should have some damping when the missile guidance system has inherent lagging, and thetransfer parameter mentioned above should not be too large when the attitude pursuit guidance law isused.
基金Project(61273138) supported by the National Natural Science Foundation of ChinaProjects(KJ2016A169,KJ2015A242) supported by the University Natural Science Research Key Project of Anhui Province,ChinaProject(ZRC2014444) supported by the Talents Program of Anhui Science and Technology University,China
文摘In order to realize safe and accurate homing of parafoil system,a multiphase homing trajectory planning scheme is proposed according to the maneuverability and basic flight characteristics of the vehicle.In this scenario,on the basis of geometric relationship of each phase trajectory,the problem of trajectory planning is transformed to parameter optimizing,and then auxiliary population-based quantum differential evolution algorithm(AP-QDEA)is applied as a tool to optimize the objective function,and the design parameters of the whole homing trajectory are obtained.The proposed AP-QDEA combines the strengths of differential evolution algorithm(DEA)and quantum evolution algorithm(QEA),and the notion of auxiliary population is introduced into the proposed algorithm to improve the searching precision and speed.The simulation results show that the proposed AP-QDEA is proven its superior in both effectiveness and efficiency by solving a set of benchmark problems,and the multiphase homing scheme can fulfill the requirement of fixed-points and upwind landing in the process of homing which is simple in control and facile in practice as well.
基金supported by the National Natural Science Foundation of China(91216104 61503302)
文摘A smart homing guidance strategy with control saturation against a target-defender team is derived. It is noteworthy that a cooperative strategy of the target-defender team is applied,which has been proved more challenging for the homing guidance.The defender missile is launched by the target and guided by a cooperative augmented proportional navigation(APN). At the same time, the target performs a one-switch maneuver to cooperate and minimize the defender's acceleration requirement. The problem is analyzed for arbitrary-order linear dynamics of the agents in the linearized form but validated by the mathematical simulations by using nonlinear kinematics. The perfect information of three agents' states is assumed. Then, a method to deal with the target-defender team is proposed. It contains a combined performance index penalizing the miss distance relative to the target and energy consumption in the whole duration. Besides, the specific miss distance related to the defender is regarded as an inequality constraint. An analytical solution for the smart guidance strategy against the APN guided defender is derived. Meanwhile, the control saturations are introduced to get more realistic and reasonable insights to this practical target-missile-defender problem. A simple but effective iterative searching technique is proposed to determine the saturation time points. The solution provides an optimal homing strategy to evade the defender with a specific miss distance and intercept the target with the minimum miss distance in the minimum energy manner. Nonlinear two-dimensional simulation results are used to validate the theoretical analysis. By comparison with the optimal differential game guidance(ODGG) and the combined minimum effort guidance(CMEG), the superiority of this smart guidance strategy is concluded.
文摘In this paper,a new homing guidance method is used to control the flying time and falling angle for guided missiles. Through this approach,it finds the approximate solution to the quadratic equation of time-togo,which is used for the formula derivation of the flying time control command. In this guidance law design,the acceleration rate control command is adopted. The guidance law is composed of a PN guidance command and a flying time control command. Firstly,it obtains a desired falling angle with accurate guidance. Secondly,it introduces to satisfy the constraint of flying time. The flying time control requires an assumption on the future evolution of missile,which is called time-to-go. To cope with the time-varying speed of missiles,a method of compensating the estimation of time-to-go is presented. The new guidance law is evaluated by using a simulation of typical terminal guidance for rocket-propelled torpedo. The simulation results show that the guidance achieves excellent control performance and exhibits insensitivity to initial trajectory parameter over a widen flight envelope.
基金Supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico(BR),No.CNPq400856/2016-6Sao Paulo State Research Support Foundation,No.FAPESP:2014/50983-3 and No.FAPESP:2016/21470-3。
文摘BACKGROUND Stroke is the second leading cause of death worldwide.There is a real need to develop treatment strategies for reducing neurological deficits in stroke survivors,and stem cell(SC)therapeutics appear to be a promising alternative for stroke therapy that can be used in combination with approved thrombolytic or thrombectomy approaches.However,the efficacy of SC therapy depends on the SC homing ability and engraftment into the injury site over a long period of time.Nonetheless,tracking SCs from their niche to the target tissues is a complex process.AIM To evaluate SC migration homing,tracking and therapeutic efficacy in the treatment of stroke using nanoparticles METHODS A systematic literature search was performed to identify articles published prior to November 2019 that were indexed in PubMed and Scopus.The following inclusion criteria were used:(1)Studies that used in vivo models of stroke or ischemic brain lesions;(2)Studies of SCs labeled with some type of contrast agent for cell migration detection;and(3)Studies that involved in vivo cellular homing and tracking analysis.RESULTS A total of 82 articles were identified by indexing in Scopus and Pub Med.Afterthe inclusion criteria were applied,35 studies were selected,and the articles were assessed for eligibility;ultimately,only 25 studies were included.Most of the selected studies used SCs from human and mouse bone marrow labeled with magnetic nanoparticles alone or combined with fluorophore dyes.These cells were administered in the stroke model(to treat middle cerebral artery occlusion in 74%of studies and for photothrombotic induction in 26%of studies).Fiftythree percent of studies used xenogeneic grafts for cell therapy,and the migration homing and tracking evaluation was performed by magnetic resonance imaging as well as other techniques,such as near-infrared fluorescence imaging(12%)or bioluminescence assays(12%).CONCLUSION Our systematic review provided an up-to-date evaluation of SC migration homing and the efficacy of cellular therapy for stroke treatment in terms of functional and structural improvements in the late stage.
基金Project(61273138)supported by the National Natural Science Foundation of ChinaProject(14JCZDJC39300)supported by the Key Fund of Tianjin,China
文摘Homing trajectory planning is a core task of autonomous homing of parafoil system.This work analyzes and establishes a simplified kinematic mathematical model,and regards the homing trajectory planning problem as a kind of multi-objective optimization problem.Being different from traditional ways of transforming the multi-objective optimization into a single objective optimization by weighting factors,this work applies an improved non-dominated sorting genetic algorithm Ⅱ(NSGA Ⅱ) to solve it directly by means of optimizing multi-objective functions simultaneously.In the improved NSGA Ⅱ,the chaos initialization and a crowding distance based population trimming method were introduced to overcome the prematurity of population,the penalty function was used in handling constraints,and the optimal solution was selected according to the method of fuzzy set theory.Simulation results of three different schemes designed according to various practical engineering requirements show that the improved NSGA Ⅱ can effectively obtain the Pareto optimal solution set under different weighting with outstanding convergence and stability,and provide a new train of thoughts to design homing trajectory of parafoil system.
文摘The strapdown homing guidance system for some ammunition was mainly studied. A strong tracking Kalman filter was designed for the strapdown homing guidance system using the information measured by the strapdown homing seeker to estimate relative movement variables between the ammunition and target. Then the optimal proportional law, which using the estimated information, guided the ammunition. Simulation results show that the designed strapdown homing guidance system with strong tracking Kalman filter can attack the maneuvering target effectively, and satisfy the performance index for the guided ammunition system.
基金Supported by Grants from the National Natural Science Foundation of China Project of Guiding Traditional Chinese Medicine Induced Bone Marrow Stem Cell Directional Homing to a Focal Zone for the Treatment of Osteoarthritis(No.81072900)
文摘OBJECTIVE: To investigate the effects of Ermiao Fang(EM) with medical guide Xixin(Herba Asari Mandshurici)(HAM) on bone marrow stem cell migration to a focal zone in osteoarthritis(OA) rats.METHODS: OA rats were induced by arthrectomy and assigned to sham-operated, model, EM, or EM plus HAM groups.All rats were injected with recombinant human granulocyte colony-stimulating factor 30μg·kg-1·d-1for7 days and treated with EMor EM plus HAM at 1.6 or 1.9 g·kg-1·d-1 for 3 or 6 weeks, respectively. Chondrocyte apoptosis and cartilage matrix components were tested by transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assay and special staining. Levels of interleukin-1 beta(IL-1β) tumor necrosis factor alpha(TNF-α) nitric oxide(NO), and inducible nitric oxide synthase(iNOS) in serum were detected by enzyme-linked immunosorbent assay or radioimmunoassay. Matrix metalloproteinases(MMPs)-13,tissue inhibitors of metalloproteinases(TIMPs)-1,Bromodeoxyuridine(BrdU), cluster of differentiation 34(CD34), and stromal cell-derived factor 1(SDF-1) were measured by immunohistochemical assay.RESULTS:The EM and EM plus HAM groups had significantly less cartilage damage and synovium inflammation the model group. Moreover, the EM and EM plus HAM groups had less chondrocyte apoptosis and more proteoglycan and collagen content than the model group.The EM and EMplus HAM groups had obviously higher MMPs-13 and TIMPs-1 expression in the cartilage than the model group. Moreover, the two formula groups had less release of IL-1β, TNF-α, NO, and iNOS than model group. Importantly, the expressions of BrdU, CD34,and SDF-1 in cartilage were significantly higher in the EM and EM plus HAM-Medtreated rats than model group. Notably, the EM plus HAM treatment seemed to have the greatest effects.CONCLUSION: HAM improves the therapeutic effects of EM on OA rats by enhancing BMSC directional homing to the focal zone.
文摘Background:Efficient and safe movement is fundamental for wild birds to thrive in their environments.For arboreal forest animals,especially birds,canopy cover has a large impact on birds’daily movements and is a crucial component of conservation strategies seeking to retain avian population in disturbed or urban habitats.Methods:We translocated woodland bird species utilizing different forest strata during two non-breeding seasons in Gainesville,FL,USA.We used linear model and generalized linear model to examine the effects of canopy cover and species identity on homing success and speed.Results:Among our study species of Tufted Titmouse(Baeolophus bicolor),Carolina Chickadee(Poecile carolinensis),and Northern Cardinal(Cardinalis cardinalis),we found that Carolina Chickadees and Tufted Titmice were more likely to return than Northern Cardinals.Among birds that successfully returned,homing speed is significantly affected by forest canopy cover and species identity(titmice had higher homing speed than cardinals).Birds return much faster in landscape with higher canopy cover.Conclusions:This study presented evidence of species identity’s effect on homing success and speed in common feeder bird species in Southeast US and provided further evidence that bird movements in the suburban land cover are constrained by low canopy cover.
