Background:Bacterial infection,tissue hypoxia and inflammatory response can hinder infected wound repair.This study aimed to develop a multifunctional specific therapeutic photo activated release nanosystem[HMPB@MB@Au...Background:Bacterial infection,tissue hypoxia and inflammatory response can hinder infected wound repair.This study aimed to develop a multifunctional specific therapeutic photo activated release nanosystem[HMPB@MB@AuNPs@PMB@HA(HMAPH)]by loading photosen sitizer methylene blue(MB)into hollow mesoporous Prussian blue nanostructures and modifying the surface with gold particles,polymyxin B(PMB)and hydrophilic hyaluronic acid.Methods:The HMAPH was characterized using transmission electron microscopy,UV-vis,Fourier transform infrared spectroscopy,X-ray diffraction and X-ray photon spectroscopy.The photother mal performance,iron ion release and free radical generation of the HMAPH were measured under different conditions to investigate its thermo-responsive cascade reaction.The antibacterial ability of HMAPH was investigated using live/dead fluorescence tests.The morphology and membrane integrity of Pseudomonas aeruginosa(P.aeruginosa)were investigated using transmission electron microscopy.The anti-biofilm activity of HMAPH was evaluated using crystal violet and SYBR Green I staining.Finally,we established a mouse model of a skin wound infected by P.aeruginosa to confirm the in vivo effectiveness of HMAPH.We used immunofluorescent staining,hematoxylin-eosin staining,Masson staining and enzyme-linked immunosorbent assay to examine whether HMAPH promoted wound healing and reduced inflammatory damage.Results:In this study,hyaluronic acid was decomposed under the action of hyaluronidase.Also,the exposed nanomaterials specifically bound to the outer membrane of P.aeruginosa through PMB to increase the membrane sensitivity to photodynamic treatment.Under dual-light irradiation,a large amount of iron ions released by HMAPH underwent a Fenton reaction with H_(2)O_(2)in bacteria to generate hydroxyl radicals(·OH),enabling direct killing of cells by hyperthermia.Additionally,the photodynamic activity of MB released by photo-induced activation led to the generation of reactive oxygen species,achieving synergistic and effective inhibition of P.aeruginosa.HMAPH also inhibited biofilm formation and downregulated the expression of virulence factors.In vivo experiments revealed that HMAPH accelerated the healing of P.aeruginosa-infected wounds by promoting angiogenesis and skin regeneration,inhibiting the inflammatory response and promoting M1 to M2 polarization.Conclusions:Our study proposed a strategy against bacteria and biofilms through a synergistic photothermal-photodynamic-Fenton reaction,opening up new prospects for combating biofilmassociated infections.展开更多
基金supported by the National Key R&D Program of China(2023YFF1103900)National Natural Science Foundation of China(31902304,31972167)the Open Project Program of State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology(SKLFNS-KF-202209)。
文摘Background:Bacterial infection,tissue hypoxia and inflammatory response can hinder infected wound repair.This study aimed to develop a multifunctional specific therapeutic photo activated release nanosystem[HMPB@MB@AuNPs@PMB@HA(HMAPH)]by loading photosen sitizer methylene blue(MB)into hollow mesoporous Prussian blue nanostructures and modifying the surface with gold particles,polymyxin B(PMB)and hydrophilic hyaluronic acid.Methods:The HMAPH was characterized using transmission electron microscopy,UV-vis,Fourier transform infrared spectroscopy,X-ray diffraction and X-ray photon spectroscopy.The photother mal performance,iron ion release and free radical generation of the HMAPH were measured under different conditions to investigate its thermo-responsive cascade reaction.The antibacterial ability of HMAPH was investigated using live/dead fluorescence tests.The morphology and membrane integrity of Pseudomonas aeruginosa(P.aeruginosa)were investigated using transmission electron microscopy.The anti-biofilm activity of HMAPH was evaluated using crystal violet and SYBR Green I staining.Finally,we established a mouse model of a skin wound infected by P.aeruginosa to confirm the in vivo effectiveness of HMAPH.We used immunofluorescent staining,hematoxylin-eosin staining,Masson staining and enzyme-linked immunosorbent assay to examine whether HMAPH promoted wound healing and reduced inflammatory damage.Results:In this study,hyaluronic acid was decomposed under the action of hyaluronidase.Also,the exposed nanomaterials specifically bound to the outer membrane of P.aeruginosa through PMB to increase the membrane sensitivity to photodynamic treatment.Under dual-light irradiation,a large amount of iron ions released by HMAPH underwent a Fenton reaction with H_(2)O_(2)in bacteria to generate hydroxyl radicals(·OH),enabling direct killing of cells by hyperthermia.Additionally,the photodynamic activity of MB released by photo-induced activation led to the generation of reactive oxygen species,achieving synergistic and effective inhibition of P.aeruginosa.HMAPH also inhibited biofilm formation and downregulated the expression of virulence factors.In vivo experiments revealed that HMAPH accelerated the healing of P.aeruginosa-infected wounds by promoting angiogenesis and skin regeneration,inhibiting the inflammatory response and promoting M1 to M2 polarization.Conclusions:Our study proposed a strategy against bacteria and biofilms through a synergistic photothermal-photodynamic-Fenton reaction,opening up new prospects for combating biofilmassociated infections.
