To investigate the function of HLA-class Ⅱ genes in the autoimmune response of insulin dependent diabetes mellitus(IDDM) , the HLA-class Ⅱ gene of IDDM patients was introduced into Ltk-cells with pSV2-neo plasmid,us...To investigate the function of HLA-class Ⅱ genes in the autoimmune response of insulin dependent diabetes mellitus(IDDM) , the HLA-class Ⅱ gene of IDDM patients was introduced into Ltk-cells with pSV2-neo plasmid,using the calcium phosphate precipitation technique. We obtained a stable cell line expressing the HLA-class Ⅱ gene from lymphocytes of IDDM patients. Expression was identified by direct ox erythocyte-CrCl3-HLA DR monoclonal antibody rosetting.展开更多
文摘To investigate the function of HLA-class Ⅱ genes in the autoimmune response of insulin dependent diabetes mellitus(IDDM) , the HLA-class Ⅱ gene of IDDM patients was introduced into Ltk-cells with pSV2-neo plasmid,using the calcium phosphate precipitation technique. We obtained a stable cell line expressing the HLA-class Ⅱ gene from lymphocytes of IDDM patients. Expression was identified by direct ox erythocyte-CrCl3-HLA DR monoclonal antibody rosetting.
