目的 分析HIV/AIDS患者衰弱的研究热点,为该领域的研究提供临床参考和理论基础。方法 检索Web of Science(WOS)核心合集数据库2015年1月至2025年1月发表的HIV/AIDS患者衰弱相关的文献,采用CiteSpace软件对文献进行可视化分析。结果 共纳...目的 分析HIV/AIDS患者衰弱的研究热点,为该领域的研究提供临床参考和理论基础。方法 检索Web of Science(WOS)核心合集数据库2015年1月至2025年1月发表的HIV/AIDS患者衰弱相关的文献,采用CiteSpace软件对文献进行可视化分析。结果 共纳入251篇文献,在国家层面,发文量居首位的为美国(135篇),中国的发文量相对较少(10篇)。中介中心性最高的为南非(0.82)。在机构层面,发文量最高的为约翰斯·霍普金斯大学(51篇),中介中心性(0.10)仅次于伦敦大学(0.12)。关键词共现分析频次最高的前三个为older adults(老年人)、infection(感染)、phenotype(表型);关键词聚类共有13个标签;关键词突现结果显示近几年研究的关键词为depression(抑郁)、challenges(挑战)、index(指数)、people with HIV(HIV感染者)、people(人群)、predictor(预测因子)。结论 老年人是HIV/AIDS衰弱研究领域的核心人群,该领域的研究热点围绕优化老年HIV/AIDS患者的共病管理、提升cART效果、开发HIV/AIDS患者衰弱针对性评估工具展开。未来研究需加强HIV/AIDS患者社会心理因素的关注力度,贯彻落实以患者为中心的护理,提高预测因子识别HIV/AIDS患者衰弱的准确性,开展多学科合作。展开更多
Despite effective antiretroviral therapy(ART),many individuals with human immunodeficiency virus(HIV)/acquired immune deficiency syndrome(AIDS)achieve viral suppression but fail to fully restore cluster of differentia...Despite effective antiretroviral therapy(ART),many individuals with human immunodeficiency virus(HIV)/acquired immune deficiency syndrome(AIDS)achieve viral suppression but fail to fully restore cluster of differentiation 4(CD4)^(+)T lymphocyte(CD4 cell)counts—a condition known as immunological non-response(INRs).INRs are associated with elevated health risks,including increased susceptibility to AIDS-related and non-AIDS-related complications.The pathogenesis of INRs remains incompletely understood,and no established therapeutic interventions exist,posing a major challenge in contemporary HIV/AIDS management.Emerging evidence indicates that INRs exhibit significant alterations in gut microbiota composition.Dysbiosis of the gut microbiota may contribute to persistent immune activation,cytokine imbalance,and cellular pyroptosis,all of which could impair immune reconstitution in people living with HIV/AIDS.Traditional Chinese medicine(TCM)has demonstrated potential immunomodulatory effects and is increasingly utilized in the management of INRs.Targeting the gut microbiota and elucidating the mechanisms by which TCM modulates this microbial ecosystem may offer new avenues for preventing and treating INRs.This review explores the interplay between gut microbiota and TCM,examines the association between gut dysbiosis and INRs,discusses the mechanistic pathways through which microbiota imbalance contributes to INRs development,and highlights how TCM interventions regulate gut microbiota to promote immune recovery.By focusing on the gut microbiota as a therapeutic interface,this article provides novel insights into TCM-based strategies for improving outcomes in INRs and supports the development of innovative treatment approaches.展开更多
目的 基于巨噬细胞自噬探讨参芩龙清肺培元颗粒的作用机制。方法 选择人类免疫缺陷病毒(HIV)/艾滋病(AIDS)合并肺部感染痰热壅肺证患者36例,应用流式细胞术检测治疗前后患者外周血T淋巴细胞亚群中CD4^(+)及CD8^(+)T淋巴细胞计数,巨噬细...目的 基于巨噬细胞自噬探讨参芩龙清肺培元颗粒的作用机制。方法 选择人类免疫缺陷病毒(HIV)/艾滋病(AIDS)合并肺部感染痰热壅肺证患者36例,应用流式细胞术检测治疗前后患者外周血T淋巴细胞亚群中CD4^(+)及CD8^(+)T淋巴细胞计数,巨噬细胞亚群CD11b、CD86、CD206的表达及巨噬细胞自噬探针CD11b+DALGreen、CD86+DALGreen、CD206+DALGreen的表达。ELISA法检测治疗前后血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-10、转化生长因子(TGF)-β1等炎性因子的表达。采用荧光定量PCR法检测治疗前后患者外周血中组蛋白去乙酰化酶(HDAC)2 m RNA、Unc-51样激酶1(ULK1)m RNA的表达。结果 治疗后,患者CD11b、CD206、CD11b+DALGreen、CD206+DALGreen、ULK1 m RNA表达明显升高(P<0.05);CD86、TNF-α、IL-1β、HDAC2 m RNA的表达明显下降(P<0.05);CD4^(+)T淋巴细胞计数、IL-10、TGF-β1表达呈上升趋势(P>0.05)。结论 参芩龙清肺培元颗粒可能通过调节HDAC2/ULK1信号轴,促进巨噬细胞自噬,调节M1/M2的平衡,抑制炎症反应,从而发挥治疗HIV/AIDS患者合并肺部感染的作用。展开更多
文摘目的 分析HIV/AIDS患者衰弱的研究热点,为该领域的研究提供临床参考和理论基础。方法 检索Web of Science(WOS)核心合集数据库2015年1月至2025年1月发表的HIV/AIDS患者衰弱相关的文献,采用CiteSpace软件对文献进行可视化分析。结果 共纳入251篇文献,在国家层面,发文量居首位的为美国(135篇),中国的发文量相对较少(10篇)。中介中心性最高的为南非(0.82)。在机构层面,发文量最高的为约翰斯·霍普金斯大学(51篇),中介中心性(0.10)仅次于伦敦大学(0.12)。关键词共现分析频次最高的前三个为older adults(老年人)、infection(感染)、phenotype(表型);关键词聚类共有13个标签;关键词突现结果显示近几年研究的关键词为depression(抑郁)、challenges(挑战)、index(指数)、people with HIV(HIV感染者)、people(人群)、predictor(预测因子)。结论 老年人是HIV/AIDS衰弱研究领域的核心人群,该领域的研究热点围绕优化老年HIV/AIDS患者的共病管理、提升cART效果、开发HIV/AIDS患者衰弱针对性评估工具展开。未来研究需加强HIV/AIDS患者社会心理因素的关注力度,贯彻落实以患者为中心的护理,提高预测因子识别HIV/AIDS患者衰弱的准确性,开展多学科合作。
基金supported by the National Natural Science Foundation of China(No.82274474)。
文摘Despite effective antiretroviral therapy(ART),many individuals with human immunodeficiency virus(HIV)/acquired immune deficiency syndrome(AIDS)achieve viral suppression but fail to fully restore cluster of differentiation 4(CD4)^(+)T lymphocyte(CD4 cell)counts—a condition known as immunological non-response(INRs).INRs are associated with elevated health risks,including increased susceptibility to AIDS-related and non-AIDS-related complications.The pathogenesis of INRs remains incompletely understood,and no established therapeutic interventions exist,posing a major challenge in contemporary HIV/AIDS management.Emerging evidence indicates that INRs exhibit significant alterations in gut microbiota composition.Dysbiosis of the gut microbiota may contribute to persistent immune activation,cytokine imbalance,and cellular pyroptosis,all of which could impair immune reconstitution in people living with HIV/AIDS.Traditional Chinese medicine(TCM)has demonstrated potential immunomodulatory effects and is increasingly utilized in the management of INRs.Targeting the gut microbiota and elucidating the mechanisms by which TCM modulates this microbial ecosystem may offer new avenues for preventing and treating INRs.This review explores the interplay between gut microbiota and TCM,examines the association between gut dysbiosis and INRs,discusses the mechanistic pathways through which microbiota imbalance contributes to INRs development,and highlights how TCM interventions regulate gut microbiota to promote immune recovery.By focusing on the gut microbiota as a therapeutic interface,this article provides novel insights into TCM-based strategies for improving outcomes in INRs and supports the development of innovative treatment approaches.
文摘目的 基于巨噬细胞自噬探讨参芩龙清肺培元颗粒的作用机制。方法 选择人类免疫缺陷病毒(HIV)/艾滋病(AIDS)合并肺部感染痰热壅肺证患者36例,应用流式细胞术检测治疗前后患者外周血T淋巴细胞亚群中CD4^(+)及CD8^(+)T淋巴细胞计数,巨噬细胞亚群CD11b、CD86、CD206的表达及巨噬细胞自噬探针CD11b+DALGreen、CD86+DALGreen、CD206+DALGreen的表达。ELISA法检测治疗前后血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-10、转化生长因子(TGF)-β1等炎性因子的表达。采用荧光定量PCR法检测治疗前后患者外周血中组蛋白去乙酰化酶(HDAC)2 m RNA、Unc-51样激酶1(ULK1)m RNA的表达。结果 治疗后,患者CD11b、CD206、CD11b+DALGreen、CD206+DALGreen、ULK1 m RNA表达明显升高(P<0.05);CD86、TNF-α、IL-1β、HDAC2 m RNA的表达明显下降(P<0.05);CD4^(+)T淋巴细胞计数、IL-10、TGF-β1表达呈上升趋势(P>0.05)。结论 参芩龙清肺培元颗粒可能通过调节HDAC2/ULK1信号轴,促进巨噬细胞自噬,调节M1/M2的平衡,抑制炎症反应,从而发挥治疗HIV/AIDS患者合并肺部感染的作用。
