IntroductionCurrently, there are at least 850,000 people living with human immuno-deficiency virus (HIV) and acquired immuno-deficiency syndrome (AIDS) in China. Among them 16-29 years old account for about 65%. A... IntroductionCurrently, there are at least 850,000 people living with human immuno-deficiency virus (HIV) and acquired immuno-deficiency syndrome (AIDS) in China. Among them 16-29 years old account for about 65%. According to the Chinese health authorities, if the preventive measures are not effective, the figure could reach as many as 10 million by the year 2010[1]. Since there are currently no cure or vaccines for HIV/AIDS, changing people's high-risk behaviors is the main approach that is taken to slow down the epidemic. This can be done through effective HIV/AIDS education, especially for young people. In 1997, a 4-year adolescent HIV/AIDS peer education program was introduced to in Beijing and Shanghai, which was based on a successful Australian model developed by Professor Roger Short, in which senior medical students were trained and used as peer educators to teach their junior peers about HIV/AIDS[2].……展开更多
目的 基于巨噬细胞自噬探讨参芩龙清肺培元颗粒的作用机制。方法 选择人类免疫缺陷病毒(HIV)/艾滋病(AIDS)合并肺部感染痰热壅肺证患者36例,应用流式细胞术检测治疗前后患者外周血T淋巴细胞亚群中CD4^(+)及CD8^(+)T淋巴细胞计数,巨噬细...目的 基于巨噬细胞自噬探讨参芩龙清肺培元颗粒的作用机制。方法 选择人类免疫缺陷病毒(HIV)/艾滋病(AIDS)合并肺部感染痰热壅肺证患者36例,应用流式细胞术检测治疗前后患者外周血T淋巴细胞亚群中CD4^(+)及CD8^(+)T淋巴细胞计数,巨噬细胞亚群CD11b、CD86、CD206的表达及巨噬细胞自噬探针CD11b+DALGreen、CD86+DALGreen、CD206+DALGreen的表达。ELISA法检测治疗前后血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-10、转化生长因子(TGF)-β1等炎性因子的表达。采用荧光定量PCR法检测治疗前后患者外周血中组蛋白去乙酰化酶(HDAC)2 m RNA、Unc-51样激酶1(ULK1)m RNA的表达。结果 治疗后,患者CD11b、CD206、CD11b+DALGreen、CD206+DALGreen、ULK1 m RNA表达明显升高(P<0.05);CD86、TNF-α、IL-1β、HDAC2 m RNA的表达明显下降(P<0.05);CD4^(+)T淋巴细胞计数、IL-10、TGF-β1表达呈上升趋势(P>0.05)。结论 参芩龙清肺培元颗粒可能通过调节HDAC2/ULK1信号轴,促进巨噬细胞自噬,调节M1/M2的平衡,抑制炎症反应,从而发挥治疗HIV/AIDS患者合并肺部感染的作用。展开更多
目的通过Meta整合方法系统总结我国HIV/AIDS患者家庭照顾者的心理体验及应对的质性研究,为构建和完善干预措施提供依据。方法计算机检索PubMed、Embase、Web of Science、中国知网、万方数据库、维普网、中国生物医学文献数据库中关于HI...目的通过Meta整合方法系统总结我国HIV/AIDS患者家庭照顾者的心理体验及应对的质性研究,为构建和完善干预措施提供依据。方法计算机检索PubMed、Embase、Web of Science、中国知网、万方数据库、维普网、中国生物医学文献数据库中关于HIV/AIDS患者家庭照顾者照护感受、经历及应对的质性研究,检索时限从建库至2024年8月。对纳入文献根据JBI循证卫生保健中心质性研究质量评价工具进行质量评价,采用汇集性整合方法进行结果整合。结果共纳入10项研究,提炼出42个研究结果,归纳形成9个类别,综合得到4个整合结果:繁重的心理和情绪负担;角色冲突及个人成长;强烈的需求;适应和应对以维持正常生活。结论应重视HIV/AIDS患者家庭照顾者的情绪体验,为HIV/AIDS患者家庭照顾者创造支持性的环境,满足照顾者的需求,促进正性情绪和积极应对策略,提高照顾者的生活质量。展开更多
基金the Ford Foundation for their financial support to the program
文摘 IntroductionCurrently, there are at least 850,000 people living with human immuno-deficiency virus (HIV) and acquired immuno-deficiency syndrome (AIDS) in China. Among them 16-29 years old account for about 65%. According to the Chinese health authorities, if the preventive measures are not effective, the figure could reach as many as 10 million by the year 2010[1]. Since there are currently no cure or vaccines for HIV/AIDS, changing people's high-risk behaviors is the main approach that is taken to slow down the epidemic. This can be done through effective HIV/AIDS education, especially for young people. In 1997, a 4-year adolescent HIV/AIDS peer education program was introduced to in Beijing and Shanghai, which was based on a successful Australian model developed by Professor Roger Short, in which senior medical students were trained and used as peer educators to teach their junior peers about HIV/AIDS[2].……
文摘目的 基于巨噬细胞自噬探讨参芩龙清肺培元颗粒的作用机制。方法 选择人类免疫缺陷病毒(HIV)/艾滋病(AIDS)合并肺部感染痰热壅肺证患者36例,应用流式细胞术检测治疗前后患者外周血T淋巴细胞亚群中CD4^(+)及CD8^(+)T淋巴细胞计数,巨噬细胞亚群CD11b、CD86、CD206的表达及巨噬细胞自噬探针CD11b+DALGreen、CD86+DALGreen、CD206+DALGreen的表达。ELISA法检测治疗前后血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-10、转化生长因子(TGF)-β1等炎性因子的表达。采用荧光定量PCR法检测治疗前后患者外周血中组蛋白去乙酰化酶(HDAC)2 m RNA、Unc-51样激酶1(ULK1)m RNA的表达。结果 治疗后,患者CD11b、CD206、CD11b+DALGreen、CD206+DALGreen、ULK1 m RNA表达明显升高(P<0.05);CD86、TNF-α、IL-1β、HDAC2 m RNA的表达明显下降(P<0.05);CD4^(+)T淋巴细胞计数、IL-10、TGF-β1表达呈上升趋势(P>0.05)。结论 参芩龙清肺培元颗粒可能通过调节HDAC2/ULK1信号轴,促进巨噬细胞自噬,调节M1/M2的平衡,抑制炎症反应,从而发挥治疗HIV/AIDS患者合并肺部感染的作用。
文摘目的通过Meta整合方法系统总结我国HIV/AIDS患者家庭照顾者的心理体验及应对的质性研究,为构建和完善干预措施提供依据。方法计算机检索PubMed、Embase、Web of Science、中国知网、万方数据库、维普网、中国生物医学文献数据库中关于HIV/AIDS患者家庭照顾者照护感受、经历及应对的质性研究,检索时限从建库至2024年8月。对纳入文献根据JBI循证卫生保健中心质性研究质量评价工具进行质量评价,采用汇集性整合方法进行结果整合。结果共纳入10项研究,提炼出42个研究结果,归纳形成9个类别,综合得到4个整合结果:繁重的心理和情绪负担;角色冲突及个人成长;强烈的需求;适应和应对以维持正常生活。结论应重视HIV/AIDS患者家庭照顾者的情绪体验,为HIV/AIDS患者家庭照顾者创造支持性的环境,满足照顾者的需求,促进正性情绪和积极应对策略,提高照顾者的生活质量。
