Deblending is a data processing procedure used to separate the source interferences of blended seismic data,which are obtained by simultaneous sources with random time delays to reduce the cost of seismic acquisition....Deblending is a data processing procedure used to separate the source interferences of blended seismic data,which are obtained by simultaneous sources with random time delays to reduce the cost of seismic acquisition.There are three types of deblending algorithms,i.e.,filtering-type noise suppression algorithm,inversion-based algorithm and deep-learning based algorithm.We review the merits of these techniques,and propose to use a sparse inversion method for seismic data deblending.Filtering-based deblending approach is applicable to blended data with a low blending fold and simple geometry.Otherwise,it can suffer from signal distortion and noise leakage.At present,the deep learning based deblending methods are still under development and field data applications are limited due to the lack of high-quality training labels.In contrast,the inversion-based deblending approaches have gained industrial acceptance.Our used inversion approach transforms the pseudo-deblended data into the frequency-wavenumber-wavenumher(FKK)domain,and a sparse constraint is imposed for the coherent signal estimation.The estimated signal is used to predict the interference noise for subtraction from the original pseudo-deblended data.Via minimizing the data misfit,the signal can be iteratively updated with a shrinking threshold until the signal and interference are fully separated.The used FKK sparse inversion algorithm is very accurate and efficient compared with other sparse inversion methods,and it is widely applied in field cases.Synthetic example shows that the deblending error is less than 1%in average amplitudes and less than-40 dB in amplitude spectra.We present three field data examples of land,marine OBN(Ocean Bottom Nodes)and streamer acquisitions to demonstrate its successful applications in separating the source interferences efficiently and accurately.展开更多
The yeast Pichia pastoris(P. pastoris) has been used for the expression of heterologous proteins with the significant success. However, it is time-consuming to screen the high expression level of the recombinant P. pa...The yeast Pichia pastoris(P. pastoris) has been used for the expression of heterologous proteins with the significant success. However, it is time-consuming to screen the high expression level of the recombinant P. pastoris directly. Thus, for β-mannanase production, developing the accurate, rapid and inexpensive screening method to substitute random screening is certainly required. A simple method based on the size of hydrolysis hole was described here, but this method was not very accurate that could only be used in preliminary screening. To further improve the accuracy, a micro-plate screening method is established, which appears to be more accurate and effective. The efficiency of this screening method is about 10 times higher than that of the general screening strategy of cultivation in shaking flasks. Two methods presented here can also be used for screening of recombinant Pichia strains with high-level expression of other heterologous protein after modification.展开更多
Since the approval to establishment,the Changning-Weiyuan National Shale Gas Demonstration Area in the Sichuan Basin(hereinafter referred to as Demonstration Area)has been built up into a shale gas industry demonstrat...Since the approval to establishment,the Changning-Weiyuan National Shale Gas Demonstration Area in the Sichuan Basin(hereinafter referred to as Demonstration Area)has been built up into a shale gas industry demonstration base characterized with scale,technology,management and green after more than 10 years of exploration and practice.Its successful experience is of great guiding significance to the efficient development of unconventional oil and gas resources in China.In this paper,the technologies which were developed for the beneficial development of the Demonstration Area during the 13th Five-Year Plan are systematically reviewed and summarized.Then,some countermeasures are proposed to solve the difficulties and challenges to the long-term benefit and stable production of middle-deep shale gas in this area.And the following research results are obtained.First,during the construction of the Demonstration Area,the“three-control”shale gas enrichment and high-production theory is innovatively proposed,and the shale gas enrichment model in the southern Sichuan Basin is clarified.Second,a high-production well cultivation method based on geology-engineering integration is established by developing six main technologies for shale gas exploration and development,which effectively supports the large-scale beneficial development of shale gas in the Demonstration Area.Third,the beneficial development model of middle-deep shale gas in the southern Sichuan Basin is preliminarily formed,and the benefit and efficiency of productivity construction are improved comprehensively.Fourth,the three measures of“controlling the decline rate,enhancing the reserve production and expanding the periphery”are used to support the replacement and stable production of middle-deep shale gas.In conclusion,to sustainably ensure the long-term benefit and stable production of shale gas in the southern Sichuan Basin,it is still necessary to deepen fine reservoir description and complex mechanism understanding and explore EOR technologies.What’s more,the important mean to improve the single-well shale gas production rate in this area is to strengthen the fine fracturing design and implementation under the deep integration of geology and engineering and improve the construction quality of fracturing network continuously.Furthermore,the only way to the low-cost development of the demonstration area is to promote the management reform and innovation continuously and improve the beneficial development model further.展开更多
基金supported by National Science and Technology Major Project(Grant No.2017ZX05018-001)。
文摘Deblending is a data processing procedure used to separate the source interferences of blended seismic data,which are obtained by simultaneous sources with random time delays to reduce the cost of seismic acquisition.There are three types of deblending algorithms,i.e.,filtering-type noise suppression algorithm,inversion-based algorithm and deep-learning based algorithm.We review the merits of these techniques,and propose to use a sparse inversion method for seismic data deblending.Filtering-based deblending approach is applicable to blended data with a low blending fold and simple geometry.Otherwise,it can suffer from signal distortion and noise leakage.At present,the deep learning based deblending methods are still under development and field data applications are limited due to the lack of high-quality training labels.In contrast,the inversion-based deblending approaches have gained industrial acceptance.Our used inversion approach transforms the pseudo-deblended data into the frequency-wavenumber-wavenumher(FKK)domain,and a sparse constraint is imposed for the coherent signal estimation.The estimated signal is used to predict the interference noise for subtraction from the original pseudo-deblended data.Via minimizing the data misfit,the signal can be iteratively updated with a shrinking threshold until the signal and interference are fully separated.The used FKK sparse inversion algorithm is very accurate and efficient compared with other sparse inversion methods,and it is widely applied in field cases.Synthetic example shows that the deblending error is less than 1%in average amplitudes and less than-40 dB in amplitude spectra.We present three field data examples of land,marine OBN(Ocean Bottom Nodes)and streamer acquisitions to demonstrate its successful applications in separating the source interferences efficiently and accurately.
基金Project(CX2012B124)supported by the Graduate Degree Thesis Innovation Program of Hunan ProvinceChina+3 种基金Project(13JJ9002)supported by the Natural Science Foundation of Hunan ProvinceChinaProject(2012XK4081)supported by the Key Science and Technology Plan of Hunan Provincial Science&Technology DepartmentChina
文摘The yeast Pichia pastoris(P. pastoris) has been used for the expression of heterologous proteins with the significant success. However, it is time-consuming to screen the high expression level of the recombinant P. pastoris directly. Thus, for β-mannanase production, developing the accurate, rapid and inexpensive screening method to substitute random screening is certainly required. A simple method based on the size of hydrolysis hole was described here, but this method was not very accurate that could only be used in preliminary screening. To further improve the accuracy, a micro-plate screening method is established, which appears to be more accurate and effective. The efficiency of this screening method is about 10 times higher than that of the general screening strategy of cultivation in shaking flasks. Two methods presented here can also be used for screening of recombinant Pichia strains with high-level expression of other heterologous protein after modification.
基金supported by the National Major Science and Technology Project“Changning-Weiyuan shale gas development demonstration project”(No.2016ZX05062)the Major Science and Technology Project of PetroChina Company Limited“Research and application of key technologies for producing 30 billion cubic meters of natural gas in Southwest Oil and Gas Field”(No.2016E-0611).
文摘Since the approval to establishment,the Changning-Weiyuan National Shale Gas Demonstration Area in the Sichuan Basin(hereinafter referred to as Demonstration Area)has been built up into a shale gas industry demonstration base characterized with scale,technology,management and green after more than 10 years of exploration and practice.Its successful experience is of great guiding significance to the efficient development of unconventional oil and gas resources in China.In this paper,the technologies which were developed for the beneficial development of the Demonstration Area during the 13th Five-Year Plan are systematically reviewed and summarized.Then,some countermeasures are proposed to solve the difficulties and challenges to the long-term benefit and stable production of middle-deep shale gas in this area.And the following research results are obtained.First,during the construction of the Demonstration Area,the“three-control”shale gas enrichment and high-production theory is innovatively proposed,and the shale gas enrichment model in the southern Sichuan Basin is clarified.Second,a high-production well cultivation method based on geology-engineering integration is established by developing six main technologies for shale gas exploration and development,which effectively supports the large-scale beneficial development of shale gas in the Demonstration Area.Third,the beneficial development model of middle-deep shale gas in the southern Sichuan Basin is preliminarily formed,and the benefit and efficiency of productivity construction are improved comprehensively.Fourth,the three measures of“controlling the decline rate,enhancing the reserve production and expanding the periphery”are used to support the replacement and stable production of middle-deep shale gas.In conclusion,to sustainably ensure the long-term benefit and stable production of shale gas in the southern Sichuan Basin,it is still necessary to deepen fine reservoir description and complex mechanism understanding and explore EOR technologies.What’s more,the important mean to improve the single-well shale gas production rate in this area is to strengthen the fine fracturing design and implementation under the deep integration of geology and engineering and improve the construction quality of fracturing network continuously.Furthermore,the only way to the low-cost development of the demonstration area is to promote the management reform and innovation continuously and improve the beneficial development model further.
