Background Autophagy, a dynamic and efficient process of self-digestion in vivo, has been proven to be ben- eficial for cardiac protection during MI process via removing the additional protein and damaged organelles i...Background Autophagy, a dynamic and efficient process of self-digestion in vivo, has been proven to be ben- eficial for cardiac protection during MI process via removing the additional protein and damaged organelles in the heart. Taxifolin （Tax）, a common plant flavonoid, has been widely used for the treatment of myocardial infarction. However, the underlying mechanism of Tax is largely unknown. Methods Murine arterial cardiomyocytes HL-1 cells were pretreated with Tax and then exposed to hypoxia environment. CCK-8 was performed for the de- termination of cell viability. Monodansylcadaverine （MDC） and Microtubule-associated protein 1A/1B-light chain 3 （LC3） were analyzed by immunofluorescence staining. The relative gene expressions of Hypoxia Inducible Factor-1 （HIFI-a） and Heine Oxygenase-1 （HO-1） were determined by qRT-PCR. Results Tax at 100μm for 6 h has the maximal effect to avoid reducing the cell viability excessively and significantly abolished hypoxia-induced cell death. Both of the MDC and LC3 immunofluorescence staining revealed markedly increase of expression of autophagy with pretreatment of Tax. Finally, qRT-PCR showed the upregulation of HIFI-a and HO-1 with Tax. Conclusions Taken together, Tax might be a potential candidate for the treatment of MI by promoting cardio- myocyte autophagy via the activation of HIF 1-a and HO-1.展开更多
基金supported by Guangzhou university industry collaborative innovation major project(1561000143)Medical Scientific Research Foundation of Guangdong Province(No.A2014002)+2 种基金National Natural Sciences Foundation of China(No.81370230/81500231/81570279)Natural Sciences Foundation of Hunan Province(No.2015JJ6118)Grant from the New Xiangya Talent Project of the Third Xiangya Hospital of Central South University(No.JJ201524)
文摘Background Autophagy, a dynamic and efficient process of self-digestion in vivo, has been proven to be ben- eficial for cardiac protection during MI process via removing the additional protein and damaged organelles in the heart. Taxifolin （Tax）, a common plant flavonoid, has been widely used for the treatment of myocardial infarction. However, the underlying mechanism of Tax is largely unknown. Methods Murine arterial cardiomyocytes HL-1 cells were pretreated with Tax and then exposed to hypoxia environment. CCK-8 was performed for the de- termination of cell viability. Monodansylcadaverine （MDC） and Microtubule-associated protein 1A/1B-light chain 3 （LC3） were analyzed by immunofluorescence staining. The relative gene expressions of Hypoxia Inducible Factor-1 （HIFI-a） and Heine Oxygenase-1 （HO-1） were determined by qRT-PCR. Results Tax at 100μm for 6 h has the maximal effect to avoid reducing the cell viability excessively and significantly abolished hypoxia-induced cell death. Both of the MDC and LC3 immunofluorescence staining revealed markedly increase of expression of autophagy with pretreatment of Tax. Finally, qRT-PCR showed the upregulation of HIFI-a and HO-1 with Tax. Conclusions Taken together, Tax might be a potential candidate for the treatment of MI by promoting cardio- myocyte autophagy via the activation of HIF 1-a and HO-1.
