Male factors contribute to 50%of infertility cases,with 20%–30%of cases being solely attributed to male infertility.Helicase for meiosis 1(HFM1)plays a crucial role in ensuring proper crossover formation and synapsis...Male factors contribute to 50%of infertility cases,with 20%–30%of cases being solely attributed to male infertility.Helicase for meiosis 1(HFM1)plays a crucial role in ensuring proper crossover formation and synapsis of homologous chromosomes during meiosis,an essential process in gametogenesis.HFM1 gene mutations are associated with male infertility,particularly in cases of non-obstructive azoospermia and severe oligozoospermia.However,the effects of intracytoplasmic sperm injection(ICSI)in HFM1-related infertility cases remain inadequately explored.This study identified novel biallelic HFM1 variants through whole-exome sequencing(WES)in a Chinese patient with severe oligozoospermia,which was confirmed by Sanger sequencing.The pathogenicity of these variants was assessed using real-time quantitative polymerase chain reaction(RT-qPCR)and immunoblotting,which revealed a significant reduction in HFM1 mRNA and protein levels in spermatozoa compared to those in a healthy control.Transmission electron microscopy revealed morphological abnormalities in sperm cells,including defects in the head and flagellum.Despite these abnormalities,ICSI treatment resulted in a favorable fertility outcome for the patient,indicating that assisted reproductive techniques(ART)can be effective in managing HFM1-related male infertility.These findings offer valuable insights into the management of such cases.展开更多
针对复杂水声信道下的水声通信系统,提出一种有效对抗频率扩展的同步方法。该方法充分利用HFM(Hyperbolic Frequency Modulation,HFM)的相关及多普勒容限特性,构造HFM特殊组合信号,达到快速、精确的时间同步。通过模拟海上信道试验数据...针对复杂水声信道下的水声通信系统,提出一种有效对抗频率扩展的同步方法。该方法充分利用HFM(Hyperbolic Frequency Modulation,HFM)的相关及多普勒容限特性,构造HFM特殊组合信号,达到快速、精确的时间同步。通过模拟海上信道试验数据分析,该方法相比传统同步方法同步误差小、计算复杂度低、同步速度快。通过DSP及海上实验结果论证方法有效可靠性。展开更多
针对水声信道时变、频变、强噪声及可用频带资源有限的特点,提出一种水下多用户通信方法。通过分析研究恒包络零自相关(Const Amplitude Zero Auto-Corelation,CAZAC)序列信号、双曲调频(Hyperbolic Frequency Modulated,HFM)信号在水...针对水声信道时变、频变、强噪声及可用频带资源有限的特点,提出一种水下多用户通信方法。通过分析研究恒包络零自相关(Const Amplitude Zero Auto-Corelation,CAZAC)序列信号、双曲调频(Hyperbolic Frequency Modulated,HFM)信号在水声信道下的扩展特性,设计一种采用CAZAC序列调制HFM信号的多用户水声通信方法。该方法不需前导信号,在同一频带内可实现对多个水声通信用户的识别及通信,且接收端通过相关检测处理具有优良的抗多途、抗多普勒频移特性。对8个用户在典型的水声信道多途模型下进行了仿真,结果表明该方法在水下平台相对运动3 m·s^(-1)、信噪比5 dB的条件下能够实现对多个用户的可靠水声通信。展开更多
In this paper,we address 3D inverse Cauchy issues of highly nonlinear elliptic equations in large cuboids by utilizing the new 3D homogenization functions of different orders to adapt all the specified boundary data.W...In this paper,we address 3D inverse Cauchy issues of highly nonlinear elliptic equations in large cuboids by utilizing the new 3D homogenization functions of different orders to adapt all the specified boundary data.We also add the average classification as an approximate solution to the nonlinear operator part,without requiring to cope with nonlinear equations to resolve the weighting coefficients because these constructions are owned many conditions about the true solution.The unknown boundary conditions and the result can be retrieved straightway by coping with a small-scale linear system when the outcome is described by a new 3D homogenization function,which is right to find the numerical solutions with the errors smaller than the level of noise being put on the over-specified Neumann conditions on the bottom of the cuboid.Besides,note that the new homogenization functions method(HFM)does not require dealing with the regularization and highly nonlinear equations.The robustness and accuracy of the HFM are verified by comparing the recovered results of several numerical experiments to the exact solutions in the entire region,even though a very large level of noise 50%is imposed on the over specified Neumann conditions.The numerical errors of our scheme are in the order of O(10^(−1))-O(10^(−4)).展开更多
基金supported by the National Natural Science Foundation of China(No.32288101,No.32370654,No.32100480,and No.32200485)Shanghai Municipal Science and Technology Major Project(No.2017SHZDZX01)。
文摘Male factors contribute to 50%of infertility cases,with 20%–30%of cases being solely attributed to male infertility.Helicase for meiosis 1(HFM1)plays a crucial role in ensuring proper crossover formation and synapsis of homologous chromosomes during meiosis,an essential process in gametogenesis.HFM1 gene mutations are associated with male infertility,particularly in cases of non-obstructive azoospermia and severe oligozoospermia.However,the effects of intracytoplasmic sperm injection(ICSI)in HFM1-related infertility cases remain inadequately explored.This study identified novel biallelic HFM1 variants through whole-exome sequencing(WES)in a Chinese patient with severe oligozoospermia,which was confirmed by Sanger sequencing.The pathogenicity of these variants was assessed using real-time quantitative polymerase chain reaction(RT-qPCR)and immunoblotting,which revealed a significant reduction in HFM1 mRNA and protein levels in spermatozoa compared to those in a healthy control.Transmission electron microscopy revealed morphological abnormalities in sperm cells,including defects in the head and flagellum.Despite these abnormalities,ICSI treatment resulted in a favorable fertility outcome for the patient,indicating that assisted reproductive techniques(ART)can be effective in managing HFM1-related male infertility.These findings offer valuable insights into the management of such cases.
文摘针对复杂水声信道下的水声通信系统,提出一种有效对抗频率扩展的同步方法。该方法充分利用HFM(Hyperbolic Frequency Modulation,HFM)的相关及多普勒容限特性,构造HFM特殊组合信号,达到快速、精确的时间同步。通过模拟海上信道试验数据分析,该方法相比传统同步方法同步误差小、计算复杂度低、同步速度快。通过DSP及海上实验结果论证方法有效可靠性。
文摘针对水声信道时变、频变、强噪声及可用频带资源有限的特点,提出一种水下多用户通信方法。通过分析研究恒包络零自相关(Const Amplitude Zero Auto-Corelation,CAZAC)序列信号、双曲调频(Hyperbolic Frequency Modulated,HFM)信号在水声信道下的扩展特性,设计一种采用CAZAC序列调制HFM信号的多用户水声通信方法。该方法不需前导信号,在同一频带内可实现对多个水声通信用户的识别及通信,且接收端通过相关检测处理具有优良的抗多途、抗多普勒频移特性。对8个用户在典型的水声信道多途模型下进行了仿真,结果表明该方法在水下平台相对运动3 m·s^(-1)、信噪比5 dB的条件下能够实现对多个用户的可靠水声通信。
基金This work was financially supported by the National United University[Grant Numbers T110M20600].
文摘In this paper,we address 3D inverse Cauchy issues of highly nonlinear elliptic equations in large cuboids by utilizing the new 3D homogenization functions of different orders to adapt all the specified boundary data.We also add the average classification as an approximate solution to the nonlinear operator part,without requiring to cope with nonlinear equations to resolve the weighting coefficients because these constructions are owned many conditions about the true solution.The unknown boundary conditions and the result can be retrieved straightway by coping with a small-scale linear system when the outcome is described by a new 3D homogenization function,which is right to find the numerical solutions with the errors smaller than the level of noise being put on the over-specified Neumann conditions on the bottom of the cuboid.Besides,note that the new homogenization functions method(HFM)does not require dealing with the regularization and highly nonlinear equations.The robustness and accuracy of the HFM are verified by comparing the recovered results of several numerical experiments to the exact solutions in the entire region,even though a very large level of noise 50%is imposed on the over specified Neumann conditions.The numerical errors of our scheme are in the order of O(10^(−1))-O(10^(−4)).
