High-fat diet(HFD)promotes the imbalance of gut microbiota,contributing to the development of metabolic disorders.Pomegranate juice(PJ)and inulin,as dietary polyphenol and dietary fiber respectively,have been reported...High-fat diet(HFD)promotes the imbalance of gut microbiota,contributing to the development of metabolic disorders.Pomegranate juice(PJ)and inulin,as dietary polyphenol and dietary fiber respectively,have been reported to ameliorate diet-induced gut microbiota dysbiosis and metabolic disorders.However,the combined effect of PJ and inulin on HFD-induced metabolic disorders has not been elucidated.In current study,we found that the combination of PJ and inulin prevent HFD-induced obesity,dyslipidemia,and gut microbiota dysbiosis.Moreover,the fecal levels of short-chain fatty acids(SCFAs)produced by inulin were increased after inulin combined with PJ.The levels of pomegranate polyphenol metabolites produced by PJ were also increased after PJ combined with inulin,especially pharmacokinetic analysis showed that the presence of inulin enhance the absorption level of urolithin A.Fecal metabolomic analysis found that PJ combined with inulin alter the metabolic status compared with PJ.It was also showed that the levels of SCFAs and pomegranate polyphenol metabolites among groups were correlated with gut microbiota and metabolic disorders indicators.Our results suggested that PJ combined with inulin prevent HFD-induced metabolic disorders,which may be attributed to the promotion of mutual metabolic transformation and absorption of PJ and inulin by gut microbiota.展开更多
桑枝是桑科植物桑(Mrous alba L.)的干燥嫩枝,具有祛风湿、利关节等功效,现代研究发现生物碱是桑枝中的主要活性物质。本文以高脂饮食小鼠为模型研究桑枝生物碱(SZ-A)对降脂的功效。实验结果表明:饲喂高脂饲料16周后,小鼠体重显著上升;...桑枝是桑科植物桑(Mrous alba L.)的干燥嫩枝,具有祛风湿、利关节等功效,现代研究发现生物碱是桑枝中的主要活性物质。本文以高脂饮食小鼠为模型研究桑枝生物碱(SZ-A)对降脂的功效。实验结果表明:饲喂高脂饲料16周后,小鼠体重显著上升;总生物碱干预后,SZ-A组小鼠体重显著低于模型组;理化指标分析发现,总生物碱显著降低了HFD小鼠血脂(TG、TC、LDL-C)、谷丙转氨酶、谷草转氨酶活性,有效缓解高脂饮食导致的小鼠肝脏病变;RT-PCR结果显示,总生物碱具有抑制CD36/PPARγ介导的脂质摄取,激活AMPK/ACC通路促进脂肪酸氧化,抑制SREBP1核转位减少脂质合成等降脂活性。本实验结果表明,桑枝生物碱SZ-A具有良好的降脂功效,能激活CD36/PPARγ与AMPK/ACC通路,抑制下游脂质代谢相关基因表达,发挥降脂功效和肝脏保护作用。展开更多
为了探究小球藻多糖对高脂饮食诱导的非酒精性脂肪性肝病(NAFLD)的改善作用及其机制,将30只C57BL/6小鼠分为正常对照组、高脂模型组及低、中、高剂量干预组(100/300/500 mg/kg·d^(-1)),持续干预12周,检测其血清生化指标、炎症因子...为了探究小球藻多糖对高脂饮食诱导的非酒精性脂肪性肝病(NAFLD)的改善作用及其机制,将30只C57BL/6小鼠分为正常对照组、高脂模型组及低、中、高剂量干预组(100/300/500 mg/kg·d^(-1)),持续干预12周,检测其血清生化指标、炎症因子、氧化应激参数,结合16S rRNA测序和代谢产物分析肠道菌群及短链脂肪酸变化。结果显示:中高剂量干预显著降低体重增幅(38.95-39.80 g vs 42.23 g)及肝脏指数(P<0.05),改善脂代谢(TC、TG降低32.6%~41.1%)和肝损伤指标(ALT/AST降低,P<0.05)。其机制涉及:(1)特异性调节菌群结构,恢复厚壁菌门/拟杆菌门比值,富集Faecalibacterium prausnitzii(与ALT、LPS负相关,r=-0.53~-0.62)和Alloprevotella(与SOD活性正相关,r=0.69);(2)促进短链脂肪酸生成(总SCFAs恢复81.3%);(3)修复肠道屏障(血清LPS降低41.6%)。系统揭示小球藻多糖通过“菌群-代谢物-肝轴”多靶点协同改善NAFLD,为微藻资源在代谢性疾病干预中的应用提供理论依据。展开更多
基金supported by the National Natural Science Foundation of China(32001679)the Science and Technology Research of Shaanxi Province(2021QFY07-03)+1 种基金the Fundamental Research Funds for the Central Universities(GK202103098)supported by the Scientific and Technological Achievements Commercialization Program of Shaanxi(2023-YD-CGZH-13)。
文摘High-fat diet(HFD)promotes the imbalance of gut microbiota,contributing to the development of metabolic disorders.Pomegranate juice(PJ)and inulin,as dietary polyphenol and dietary fiber respectively,have been reported to ameliorate diet-induced gut microbiota dysbiosis and metabolic disorders.However,the combined effect of PJ and inulin on HFD-induced metabolic disorders has not been elucidated.In current study,we found that the combination of PJ and inulin prevent HFD-induced obesity,dyslipidemia,and gut microbiota dysbiosis.Moreover,the fecal levels of short-chain fatty acids(SCFAs)produced by inulin were increased after inulin combined with PJ.The levels of pomegranate polyphenol metabolites produced by PJ were also increased after PJ combined with inulin,especially pharmacokinetic analysis showed that the presence of inulin enhance the absorption level of urolithin A.Fecal metabolomic analysis found that PJ combined with inulin alter the metabolic status compared with PJ.It was also showed that the levels of SCFAs and pomegranate polyphenol metabolites among groups were correlated with gut microbiota and metabolic disorders indicators.Our results suggested that PJ combined with inulin prevent HFD-induced metabolic disorders,which may be attributed to the promotion of mutual metabolic transformation and absorption of PJ and inulin by gut microbiota.
文摘桑枝是桑科植物桑(Mrous alba L.)的干燥嫩枝,具有祛风湿、利关节等功效,现代研究发现生物碱是桑枝中的主要活性物质。本文以高脂饮食小鼠为模型研究桑枝生物碱(SZ-A)对降脂的功效。实验结果表明:饲喂高脂饲料16周后,小鼠体重显著上升;总生物碱干预后,SZ-A组小鼠体重显著低于模型组;理化指标分析发现,总生物碱显著降低了HFD小鼠血脂(TG、TC、LDL-C)、谷丙转氨酶、谷草转氨酶活性,有效缓解高脂饮食导致的小鼠肝脏病变;RT-PCR结果显示,总生物碱具有抑制CD36/PPARγ介导的脂质摄取,激活AMPK/ACC通路促进脂肪酸氧化,抑制SREBP1核转位减少脂质合成等降脂活性。本实验结果表明,桑枝生物碱SZ-A具有良好的降脂功效,能激活CD36/PPARγ与AMPK/ACC通路,抑制下游脂质代谢相关基因表达,发挥降脂功效和肝脏保护作用。
文摘为了探究小球藻多糖对高脂饮食诱导的非酒精性脂肪性肝病(NAFLD)的改善作用及其机制,将30只C57BL/6小鼠分为正常对照组、高脂模型组及低、中、高剂量干预组(100/300/500 mg/kg·d^(-1)),持续干预12周,检测其血清生化指标、炎症因子、氧化应激参数,结合16S rRNA测序和代谢产物分析肠道菌群及短链脂肪酸变化。结果显示:中高剂量干预显著降低体重增幅(38.95-39.80 g vs 42.23 g)及肝脏指数(P<0.05),改善脂代谢(TC、TG降低32.6%~41.1%)和肝损伤指标(ALT/AST降低,P<0.05)。其机制涉及:(1)特异性调节菌群结构,恢复厚壁菌门/拟杆菌门比值,富集Faecalibacterium prausnitzii(与ALT、LPS负相关,r=-0.53~-0.62)和Alloprevotella(与SOD活性正相关,r=0.69);(2)促进短链脂肪酸生成(总SCFAs恢复81.3%);(3)修复肠道屏障(血清LPS降低41.6%)。系统揭示小球藻多糖通过“菌群-代谢物-肝轴”多靶点协同改善NAFLD,为微藻资源在代谢性疾病干预中的应用提供理论依据。
