Aqueous zinc-ion batteries(AZIBs) are regarded as one of the most promising energy conversion and storage devices.Nevertheless,side reactions and dendrite growth on the zinc metal anode hinder their widespread applica...Aqueous zinc-ion batteries(AZIBs) are regarded as one of the most promising energy conversion and storage devices.Nevertheless,side reactions and dendrite growth on the zinc metal anode hinder their widespread application.In this study,hemin was employed as a multi-functional artificial interface for the first time to inhibit the disordered growth of zinc dendrites and mitigate side reactions.Theoretical calculations indicate that hemin is preferentially adsorbed onto the zinc anode,thus blocking the interaction between the active zinc anode and electrolyte.Compared with zinc foil,the Hemin@Zn anode demonstrates enhanced corrosion resistance,a decrease in hydrogen evolution,and more orderly deposition of zinc.As expected,the symmetric cell with Hemin@Zn anode can sustain up to 4000 h at 0.2 mA/cm^(2),0.2 mAh/cm^(2).Asymmetric Zn//Cu cells exhibit an average coulombic efficiency exceeding 99.72 % during 500 cycles.Moreover,the full cell Hemin@Zn//NH_(4)V_(4)O_(10) delivers a superior capacity up to 367 m Ah/g and the discharge capacity retention reaches 124 mAh/g after 1200 cycles even at a current density of 5 A/g.This work provides a simple and effective method for constructing a robust artificial interface to promote the application of long-life AZIBs.展开更多
G-quadruplex DNA(G4)can function as a kind of nucleic acid apoenzyme for constructing G4/hemin biocatalyst to mimic the catalytic function of hemoprotein.However,achieving stereoselective control with G4/hemin remains...G-quadruplex DNA(G4)can function as a kind of nucleic acid apoenzyme for constructing G4/hemin biocatalyst to mimic the catalytic function of hemoprotein.However,achieving stereoselective control with G4/hemin remains a persistent challenge.Here,we report that a PW17/hemin(PW17:5’-GGGTAGGGCGGGTTGGG-3’),adopting the 5’-5’stacked dimeric parallel G4 topology,can realize the enantioselective induction in intramolecular cyclopropanation of allyl diazoacetates with enantioselectivity up to 87%ee.Spectroscopic characterization and catalytic results demonstrate that the relatively open G-quartet of the 3’terminal in dimeric PW17 contributes a catalytic pocket for hemin accommodation and plays a pivotal role in enantioselective control.This finding expands the unique repertoire of heme enzyme using biological scaffolds from proteins to nucleic acids and resolves the long-standing challenge of stereochemical control in G4/hemin catalysis.展开更多
Cryptococcosis,a serious systemic fungal infection caused by Cryptococcus neoformans(C.neoformans)and its variants,poses a significant clinical challenge due to its poor prognosis and severe health implications.The tr...Cryptococcosis,a serious systemic fungal infection caused by Cryptococcus neoformans(C.neoformans)and its variants,poses a significant clinical challenge due to its poor prognosis and severe health implications.The treatment of cryptococcal infections is complicated by several unique factors,stemming from both the pathogenic characteristics of the fungi and the biological barriers they exploit.These include the fungi’s protective capsule,their ability to reside within host macrophages—thereby evading pharmacological intervention—and their involvement in multi-organ infections such as the lung and brain,in particular their strategic positioning within the brain,protected by the blood-brain barrier(BBB).To overcome these obstacles,precise active targeting emerges as a pivotal strategy.Identifying common targets is imperative to enhance therapeutic efficacy while ensuring the druggability of delivery systems.However,research on the methodology for selecting such shared targets remains sparse.In our investigation,we have pioneered the use of secreted proteins as shared target to trace the pathogens and their infection pathways.We identified the mannoprotein Cig1,prominently expressed on the surfaces of infected macrophages,lungs,and brains,as a viable shared target.On this basis,we utilized Hemin,a ligand for Cig1,to design liposomes(Hemin Lip)tailored for addressing complex fungal infections.By leveraging the interaction with the secreted protein Cig1,Hemin Lip specifically identifies and binds to organs and macrophages harboring cryptococcal infections,thereby facilitating targeted and efficacious clearance of both intracellular and extracellular fungus.Moreover,we have extended this targeting mechanism to other nanomedicinal platforms,including albumin nanoparticles.This study proposes an innovative drug delivery model that targets extracellular secretory proteins within the infection microenvironment,offering a streamlined formulation with the potential for effective therapy against complex infections.展开更多
使用含有偕胺肟基团的改性聚丙烯腈(PAN)纤维作为载体材料,通过轴向配位作用负载氯化血红素(hemin)制备了非均相催化剂hemin-PAN,重点对其在有机染料氧化降解反应中的催化性能进行研究。结果表明,hemin-PAN能够通过活化H2O2催化染料的...使用含有偕胺肟基团的改性聚丙烯腈(PAN)纤维作为载体材料,通过轴向配位作用负载氯化血红素(hemin)制备了非均相催化剂hemin-PAN,重点对其在有机染料氧化降解反应中的催化性能进行研究。结果表明,hemin-PAN能够通过活化H2O2催化染料的氧化降解反应,其催化活性与纤维中偕胺肟基团数量密切相关,PAN增重率为14.9%时,hemin-PAN有最高的催化活性;增加催化剂hemin负载量或提高反应温度都有利于染料的氧化降解反应,hemin-PAN(增重率为14.9%,hemin负载量为0.026 mmol/g)催化罗丹明B染料氧化降解反应的活化能为63.83 k J/mol。展开更多
Hemin can improve the stress resistance of plants through the heme oxygenase system.Additionally,substances contained in plants,such as secondary metabolites,can improve stress resistance.However,few studies have expl...Hemin can improve the stress resistance of plants through the heme oxygenase system.Additionally,substances contained in plants,such as secondary metabolites,can improve stress resistance.However,few studies have explored the effects of hemin on secondary metabolite content.Therefore,the effects of hemin on saponin synthesis and the mechanism of plant injury relief by hemin in Conyza blinii were investigated in this study.Hemin treatment promoted plant growth and increased the antioxidant enzyme activity and saponin content of C.blinii under osmotic stress and cold stress.Further study showed that hemin could provide sufficient precursors for saponin synthesis by improving the photosynthetic capacity of C.blinii and increasing the gene expression of key enzymes in the saponin synthesis pathway,thus increasing the saponin content.Moreover,the promotion effect of hemin on saponin synthesis is dependent on heme oxygenase-1 and can be reversed by the inhibitor Zn-protoporphyrin-IX(ZnPPIX).This study revealed that hemin can increase the saponin content of C.blinii and alleviate the damage caused by abiotic stress,and it also broadened the understanding of the relationship between hemin and secondary metabolites in plant abiotic stress relief.展开更多
Summary: The role of HO-1 inducer, hemin, in chronic renal failure (CRF) rats and its possible mechanism of action was studied. 5/6 subtotal nephrectomy was performed to establish chronic renal failure model. Rats wer...Summary: The role of HO-1 inducer, hemin, in chronic renal failure (CRF) rats and its possible mechanism of action was studied. 5/6 subtotal nephrectomy was performed to establish chronic renal failure model. Rats were randomly assigned to 4 groups: sham-operated group, CRF group, ferrous gluconate group and hemin group. At the 10th week after operation, serum creatinine, BUN, RBC, HGB and HCT were measured. Renal pathologic changes were observed. RT-PCR and immunohistochemistry were used to detect the expression and distribution of HO-1. RT-PCR and radioimmunoassay was used to determine the expression of ET-1 in the kidney and plasma. The results showed that as compared with CRF group, serum creatinine and BUN in hemin group were reduced significantly and nephrogenic anemia was improved markedly. Glomerular mesangial proliferation and interstitial lesion were also ameliorated significantly. Hemin not only increased the expression of HO-1 but also reduced the expression of ET-1 in the kidney. The level of ET-1 protein in the plasma was also reduced after hemin treatment. Most of these indexes were not obviously changed in ferrous gluconate group. It was suggested that through inducing the expression of HO-1 and reducing the level of ET-1 in the kidney and plasma, hemin plays an important protective role in 5/6 subtotal nephrectomized rats.展开更多
Objective:To explore antischitosome effects of artemether,hemin and Fe on S/LDH.Methods: Enzyme activity of rS/LDH was assayed in the standard reaction system by adding different concentration of reagents(0.00-0.10 mM...Objective:To explore antischitosome effects of artemether,hemin and Fe on S/LDH.Methods: Enzyme activity of rS/LDH was assayed in the standard reaction system by adding different concentration of reagents(0.00-0.10 mM artemether,0.00-0.02 mM hemin,0.00-0.50 mM Fe^(3+)). Same solvents of the each reagent were used as control.Results:There was no enzyme activity inhibition observed at 0.10 mM artemther:obivious inhibition for lactate oxidation reaction and pyruvate reduction reaction were detected at 0.002 mM and 0.004 mM of hemin,respectively: comparing with that of the control(P<0.05).The relative enzymatic activity inhibitions for pyruvate reduction reaction and lactate oxidation reaction at 0.02 mM hemin were 93.48%and 100.00%,respectively,comparing with that of the control(P<0.01):both pyruvate reduction and lactate oxidation reaction were inhibited completely at 0.50 mM Fe^(3+),comparing with that of the control(P<0.01).Conclusions:The results implied that SjLDH was not the direct molecular target of artemether.Hemin and Fe are inhibitors of SjLDH.展开更多
BACKGROUND： Despite a large amount of resuscitation research, the survival rate after cardiac arrest remains low, and brain injury is the key issue. Neuroglobin （NGB） is an oxygen-binding heme protein found in the...BACKGROUND： Despite a large amount of resuscitation research, the survival rate after cardiac arrest remains low, and brain injury is the key issue. Neuroglobin （NGB） is an oxygen-binding heme protein found in the brain with a protection role against ischemic-hypoxic brain injury. Hemin is an effective activator of neuroglobin. This study was undertaken to assess the effect of hemin on expression of neuroglobin （NGB） in the cerebral cortex, neuro-defi cit score （NDS） and pathological changes after cardiopulmonary resuscitation （CPR） in rats.METHODS： A total of 120 male Sprague-Dawley （SD） rats were randomly divided into a control group （A）, a CPR group （B） and a Hemin group （C）. The animal model of cardiac arrest （CA） induced by asphyxia and CPR was established. NGB expression in the cerebral cortex with immunohistochemistry, NDS and pathological changes in the cerebral cortex were examined at 3, 6, 12, 24 hours after recovery of spontaneous circulation （ROSC） in each group. Experimental data were treated as one-factor analysis of variance and the Tukey test.RESULTS： In comparison with group A, NGB expression was increased signifi cantly at 12 and 24 hours after ROSC （P〈0.05 or P〈0.01）, NDS was decreased signifi cantly at each time point after ROSC （P〈0.01）, and pathological changes were severe at each time point after ROSC in group B. In comparison with group A, NGB expression was increased signifi cantly at 6, 12, 24 hours after ROSC （P〈0.05 or P〈0.01）, NDS was decreased signifi cantly at 3, 6, 12 hours after ROSC （P〈0.01） in group C. In comparison with group B, NGB expression was increased signifi cantly at 12 and 24 hours after ROSC, NDS was increased signifi cantly at 12 and 24 hours after ROSC, and pathological changes were milder in group C.CONCLUSION： There were increased NGB expression in the cerebral cortex, decreased NDS, and severe pathological changes after CPR in rats. Hemin treatment up-regulated expression of NGB, improved NDS, mitigated pathological changes, and alleviated cerebral injury after CPR.展开更多
基金financially supported by the National Natural Science Foundation of China (No.52372188)Natural Science Foundation of Henan (Nos.242300421625,252300421333)+4 种基金CAS Henan Industrial Technology Innovation & Incubation Center (No.2024121)Key Scientific Research Project of Education Department of Henan Province (Nos.22A150042,23A150038,and 24A150019)2023 Introduction of studying abroad talent programthe China Postdoctoral Science Foundation (No.2019 M652546)Key Project of Science and Technology of Henan Province (No.252102240007)。
文摘Aqueous zinc-ion batteries(AZIBs) are regarded as one of the most promising energy conversion and storage devices.Nevertheless,side reactions and dendrite growth on the zinc metal anode hinder their widespread application.In this study,hemin was employed as a multi-functional artificial interface for the first time to inhibit the disordered growth of zinc dendrites and mitigate side reactions.Theoretical calculations indicate that hemin is preferentially adsorbed onto the zinc anode,thus blocking the interaction between the active zinc anode and electrolyte.Compared with zinc foil,the Hemin@Zn anode demonstrates enhanced corrosion resistance,a decrease in hydrogen evolution,and more orderly deposition of zinc.As expected,the symmetric cell with Hemin@Zn anode can sustain up to 4000 h at 0.2 mA/cm^(2),0.2 mAh/cm^(2).Asymmetric Zn//Cu cells exhibit an average coulombic efficiency exceeding 99.72 % during 500 cycles.Moreover,the full cell Hemin@Zn//NH_(4)V_(4)O_(10) delivers a superior capacity up to 367 m Ah/g and the discharge capacity retention reaches 124 mAh/g after 1200 cycles even at a current density of 5 A/g.This work provides a simple and effective method for constructing a robust artificial interface to promote the application of long-life AZIBs.
文摘G-quadruplex DNA(G4)can function as a kind of nucleic acid apoenzyme for constructing G4/hemin biocatalyst to mimic the catalytic function of hemoprotein.However,achieving stereoselective control with G4/hemin remains a persistent challenge.Here,we report that a PW17/hemin(PW17:5’-GGGTAGGGCGGGTTGGG-3’),adopting the 5’-5’stacked dimeric parallel G4 topology,can realize the enantioselective induction in intramolecular cyclopropanation of allyl diazoacetates with enantioselectivity up to 87%ee.Spectroscopic characterization and catalytic results demonstrate that the relatively open G-quartet of the 3’terminal in dimeric PW17 contributes a catalytic pocket for hemin accommodation and plays a pivotal role in enantioselective control.This finding expands the unique repertoire of heme enzyme using biological scaffolds from proteins to nucleic acids and resolves the long-standing challenge of stereochemical control in G4/hemin catalysis.
基金supported by the National Natural Science Foundation of China(NSFC No.82073789)Chongqing’Special Funding for Postdoctoral Research Projects(2212013362060154)+1 种基金the Fundamental Research Funds for the Central Universities(SWU120068)the Venture and Innovation Support Program for Chongqing Over-seas Returnees(cx2022025)。
文摘Cryptococcosis,a serious systemic fungal infection caused by Cryptococcus neoformans(C.neoformans)and its variants,poses a significant clinical challenge due to its poor prognosis and severe health implications.The treatment of cryptococcal infections is complicated by several unique factors,stemming from both the pathogenic characteristics of the fungi and the biological barriers they exploit.These include the fungi’s protective capsule,their ability to reside within host macrophages—thereby evading pharmacological intervention—and their involvement in multi-organ infections such as the lung and brain,in particular their strategic positioning within the brain,protected by the blood-brain barrier(BBB).To overcome these obstacles,precise active targeting emerges as a pivotal strategy.Identifying common targets is imperative to enhance therapeutic efficacy while ensuring the druggability of delivery systems.However,research on the methodology for selecting such shared targets remains sparse.In our investigation,we have pioneered the use of secreted proteins as shared target to trace the pathogens and their infection pathways.We identified the mannoprotein Cig1,prominently expressed on the surfaces of infected macrophages,lungs,and brains,as a viable shared target.On this basis,we utilized Hemin,a ligand for Cig1,to design liposomes(Hemin Lip)tailored for addressing complex fungal infections.By leveraging the interaction with the secreted protein Cig1,Hemin Lip specifically identifies and binds to organs and macrophages harboring cryptococcal infections,thereby facilitating targeted and efficacious clearance of both intracellular and extracellular fungus.Moreover,we have extended this targeting mechanism to other nanomedicinal platforms,including albumin nanoparticles.This study proposes an innovative drug delivery model that targets extracellular secretory proteins within the infection microenvironment,offering a streamlined formulation with the potential for effective therapy against complex infections.
文摘使用含有偕胺肟基团的改性聚丙烯腈(PAN)纤维作为载体材料,通过轴向配位作用负载氯化血红素(hemin)制备了非均相催化剂hemin-PAN,重点对其在有机染料氧化降解反应中的催化性能进行研究。结果表明,hemin-PAN能够通过活化H2O2催化染料的氧化降解反应,其催化活性与纤维中偕胺肟基团数量密切相关,PAN增重率为14.9%时,hemin-PAN有最高的催化活性;增加催化剂hemin负载量或提高反应温度都有利于染料的氧化降解反应,hemin-PAN(增重率为14.9%,hemin负载量为0.026 mmol/g)催化罗丹明B染料氧化降解反应的活化能为63.83 k J/mol。
基金supported by the Sichuan Science and Technology Program(No.2020YFH0136),China。
文摘Hemin can improve the stress resistance of plants through the heme oxygenase system.Additionally,substances contained in plants,such as secondary metabolites,can improve stress resistance.However,few studies have explored the effects of hemin on secondary metabolite content.Therefore,the effects of hemin on saponin synthesis and the mechanism of plant injury relief by hemin in Conyza blinii were investigated in this study.Hemin treatment promoted plant growth and increased the antioxidant enzyme activity and saponin content of C.blinii under osmotic stress and cold stress.Further study showed that hemin could provide sufficient precursors for saponin synthesis by improving the photosynthetic capacity of C.blinii and increasing the gene expression of key enzymes in the saponin synthesis pathway,thus increasing the saponin content.Moreover,the promotion effect of hemin on saponin synthesis is dependent on heme oxygenase-1 and can be reversed by the inhibitor Zn-protoporphyrin-IX(ZnPPIX).This study revealed that hemin can increase the saponin content of C.blinii and alleviate the damage caused by abiotic stress,and it also broadened the understanding of the relationship between hemin and secondary metabolites in plant abiotic stress relief.
文摘Summary: The role of HO-1 inducer, hemin, in chronic renal failure (CRF) rats and its possible mechanism of action was studied. 5/6 subtotal nephrectomy was performed to establish chronic renal failure model. Rats were randomly assigned to 4 groups: sham-operated group, CRF group, ferrous gluconate group and hemin group. At the 10th week after operation, serum creatinine, BUN, RBC, HGB and HCT were measured. Renal pathologic changes were observed. RT-PCR and immunohistochemistry were used to detect the expression and distribution of HO-1. RT-PCR and radioimmunoassay was used to determine the expression of ET-1 in the kidney and plasma. The results showed that as compared with CRF group, serum creatinine and BUN in hemin group were reduced significantly and nephrogenic anemia was improved markedly. Glomerular mesangial proliferation and interstitial lesion were also ameliorated significantly. Hemin not only increased the expression of HO-1 but also reduced the expression of ET-1 in the kidney. The level of ET-1 protein in the plasma was also reduced after hemin treatment. Most of these indexes were not obviously changed in ferrous gluconate group. It was suggested that through inducing the expression of HO-1 and reducing the level of ET-1 in the kidney and plasma, hemin plays an important protective role in 5/6 subtotal nephrectomized rats.
基金Supported by National Nature Science Fundation of China(No. 30860070)
文摘Objective:To explore antischitosome effects of artemether,hemin and Fe on S/LDH.Methods: Enzyme activity of rS/LDH was assayed in the standard reaction system by adding different concentration of reagents(0.00-0.10 mM artemether,0.00-0.02 mM hemin,0.00-0.50 mM Fe^(3+)). Same solvents of the each reagent were used as control.Results:There was no enzyme activity inhibition observed at 0.10 mM artemther:obivious inhibition for lactate oxidation reaction and pyruvate reduction reaction were detected at 0.002 mM and 0.004 mM of hemin,respectively: comparing with that of the control(P<0.05).The relative enzymatic activity inhibitions for pyruvate reduction reaction and lactate oxidation reaction at 0.02 mM hemin were 93.48%and 100.00%,respectively,comparing with that of the control(P<0.01):both pyruvate reduction and lactate oxidation reaction were inhibited completely at 0.50 mM Fe^(3+),comparing with that of the control(P<0.01).Conclusions:The results implied that SjLDH was not the direct molecular target of artemether.Hemin and Fe are inhibitors of SjLDH.
文摘BACKGROUND： Despite a large amount of resuscitation research, the survival rate after cardiac arrest remains low, and brain injury is the key issue. Neuroglobin （NGB） is an oxygen-binding heme protein found in the brain with a protection role against ischemic-hypoxic brain injury. Hemin is an effective activator of neuroglobin. This study was undertaken to assess the effect of hemin on expression of neuroglobin （NGB） in the cerebral cortex, neuro-defi cit score （NDS） and pathological changes after cardiopulmonary resuscitation （CPR） in rats.METHODS： A total of 120 male Sprague-Dawley （SD） rats were randomly divided into a control group （A）, a CPR group （B） and a Hemin group （C）. The animal model of cardiac arrest （CA） induced by asphyxia and CPR was established. NGB expression in the cerebral cortex with immunohistochemistry, NDS and pathological changes in the cerebral cortex were examined at 3, 6, 12, 24 hours after recovery of spontaneous circulation （ROSC） in each group. Experimental data were treated as one-factor analysis of variance and the Tukey test.RESULTS： In comparison with group A, NGB expression was increased signifi cantly at 12 and 24 hours after ROSC （P〈0.05 or P〈0.01）, NDS was decreased signifi cantly at each time point after ROSC （P〈0.01）, and pathological changes were severe at each time point after ROSC in group B. In comparison with group A, NGB expression was increased signifi cantly at 6, 12, 24 hours after ROSC （P〈0.05 or P〈0.01）, NDS was decreased signifi cantly at 3, 6, 12 hours after ROSC （P〈0.01） in group C. In comparison with group B, NGB expression was increased signifi cantly at 12 and 24 hours after ROSC, NDS was increased signifi cantly at 12 and 24 hours after ROSC, and pathological changes were milder in group C.CONCLUSION： There were increased NGB expression in the cerebral cortex, decreased NDS, and severe pathological changes after CPR in rats. Hemin treatment up-regulated expression of NGB, improved NDS, mitigated pathological changes, and alleviated cerebral injury after CPR.
