The shift from skotomorphogenesis to photomorphogenesis,a developmental transition in seed plants,in-volves dramatic proteomic changes.Lysine acetylation(Lys-Ac)is an evolutionarily conserved and recog-nized post-tran...The shift from skotomorphogenesis to photomorphogenesis,a developmental transition in seed plants,in-volves dramatic proteomic changes.Lysine acetylation(Lys-Ac)is an evolutionarily conserved and recog-nized post-translational modification that plays a crucial role in plant development.However,its role in seedling deetiolation remains unclear.In this study,we conducted a comparative lysine acetylomic anal-ysis of etiolated Arabidopsis seedlings before and after red(R)light irradiation,uncovering that exposure to R light mainly led to protein lysine deacetylation during seedling deetiolation.Phytochrome A(phyA),a unique far-red(FR)light photoreceptor,was deacetylated at lysine 65(K65)when etiolated seedlings were moved to light.This residue is a critical ubiquitination site that regulates phyA stability.Moreover,K65 deacetylation facilitates phyA ubiquitination and 26s proteasome-mediated degradation,and is required for the function of phyA in FR light signaling and seedling photomorphogenesis.Furthermore,we identified a plant-specific lysine deacetylase HDT2 that interacts with and deacetylates phyA in the nu-cleus to promote its ubiquitination and degradation during seedling deetiolation.Genetic analysis revealed that HDT2 is critical for phyA-mediated photomorphogenic growth.Taken together,these findings reveal that lysine deacetylation of phyA by HDT2 plays a crucial role in modulating phyA turnover in response to light,suggesting that Lys-Ac might be central to the reprogramming of plants for photomorphogenic growth.展开更多
基金supported by grants from the National Natural Science Foundation of China(32371326 and 32070551)the Science and Technology Projects in Guangzhou(E3330900-01)the Youth Innovation PromotionAssociation,CAs(201860).
文摘The shift from skotomorphogenesis to photomorphogenesis,a developmental transition in seed plants,in-volves dramatic proteomic changes.Lysine acetylation(Lys-Ac)is an evolutionarily conserved and recog-nized post-translational modification that plays a crucial role in plant development.However,its role in seedling deetiolation remains unclear.In this study,we conducted a comparative lysine acetylomic anal-ysis of etiolated Arabidopsis seedlings before and after red(R)light irradiation,uncovering that exposure to R light mainly led to protein lysine deacetylation during seedling deetiolation.Phytochrome A(phyA),a unique far-red(FR)light photoreceptor,was deacetylated at lysine 65(K65)when etiolated seedlings were moved to light.This residue is a critical ubiquitination site that regulates phyA stability.Moreover,K65 deacetylation facilitates phyA ubiquitination and 26s proteasome-mediated degradation,and is required for the function of phyA in FR light signaling and seedling photomorphogenesis.Furthermore,we identified a plant-specific lysine deacetylase HDT2 that interacts with and deacetylates phyA in the nu-cleus to promote its ubiquitination and degradation during seedling deetiolation.Genetic analysis revealed that HDT2 is critical for phyA-mediated photomorphogenic growth.Taken together,these findings reveal that lysine deacetylation of phyA by HDT2 plays a crucial role in modulating phyA turnover in response to light,suggesting that Lys-Ac might be central to the reprogramming of plants for photomorphogenic growth.
