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Secure task offloading strategy optimization of UAV-aided outdoor mobile high-definition live streaming
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作者 Ming YAN Yuxuan ZHANG +2 位作者 Chien Aun CHAN André F.GYGAX Chunguo LI 《Chinese Journal of Aeronautics》 2025年第10期201-215,共15页
Unmanned aerial vehicles(UAVs)bring more innovation and attraction to outdoor mobile high-definition(HD)live streaming with its unique perspective.Due to the heavy computational requirements of HD live broadcast tasks... Unmanned aerial vehicles(UAVs)bring more innovation and attraction to outdoor mobile high-definition(HD)live streaming with its unique perspective.Due to the heavy computational requirements of HD live broadcast tasks and the limited hardware performance of UAV equipment,how to reduce the system response delay and improve the energy efficiency of terminal equipment directly affects the secure broadcast of the system.Secure task offloading in this scenario is considered a promising solution and has received academic attention.In this paper,we simulate the UAV-aided outdoor mobile HD live streaming scenarios and optimize the relevant task offloading strategies.First,we design the total cost function of task offloading that jointly optimizes secure time latency and energy consumption.Additionally,we propose a collaborative computing model for multi-UAV task offloading.This model combines the idea of simulated annealing(SA)and introduces the compression factor to enhance the particle swarm optimization(PSO)to realize secure task offloading.The simulation results show that the proposed strategy has better performance in balancing network latency and energy consumption.Compared with the discrete teaching–learning-based optimization(DTLBO)and quantum PSO(QPSO)task offloading strategies,the fitness value of the proposed strategy is decreased by an average of 26.73%and 16.42%,respectively. 展开更多
关键词 Unmanned aerial vehicle(UAV) high-definition(HD)live streaming Secure task offloading Network energy consumption Particle swarm optimization(PSO)
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Standard-definition White-light,High-definition White-light versus Narrow-band Imaging Endoscopy for Detecting Colorectal Adenomas:A Multicenter Randomized Controlled Trial 被引量:1
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作者 Chang-wei DUAN Hui-hong ZHAI +10 位作者 Hui XIE Xian-zong MA Dong-liang YU Lang YANG Xin WANG Yu-fen TANG Jie ZHANG Hui SU Jian-qiu SHENG Jun-feng XU Peng JIN 《Current Medical Science》 SCIE CAS 2024年第3期554-560,共7页
Objective This study aimed to compare the performance of standard-definition white-light endoscopy(SD-WL),high-definition white-light endoscopy(HD-WL),and high-definition narrow-band imaging(HD-NBI)in detecting colore... Objective This study aimed to compare the performance of standard-definition white-light endoscopy(SD-WL),high-definition white-light endoscopy(HD-WL),and high-definition narrow-band imaging(HD-NBI)in detecting colorectal lesions in the Chinese population.Methods This was a multicenter,single-blind,randomized,controlled trial with a non-inferiority design.Patients undergoing endoscopy for physical examination,screening,and surveillance were enrolled from July 2017 to December 2020.The primary outcome measure was the adenoma detection rate(ADR),defined as the proportion of patients with at least one adenoma detected.The associated factors for detecting adenomas were assessed using univariate and multivariate logistic regression.Results Out of 653 eligible patients enrolled,data from 596 patients were analyzed.The ADRs were 34.5%in the SD-WL group,33.5%in the HD-WL group,and 37.5%in the HD-NBI group(P=0.72).The advanced neoplasm detection rates(ANDRs)in the three arms were 17.1%,15.5%,and 10.4%(P=0.17).No significant differences were found between the SD group and HD group regarding ADR or ANDR(ADR:34.5%vs.35.6%,P=0.79;ANDR:17.1%vs.13.0%,P=0.16,respectively).Similar results were observed between the HD-WL group and HD-NBI group(ADR:33.5%vs.37.7%,P=0.45;ANDR:15.5%vs.10.4%,P=0.18,respectively).In the univariate and multivariate logistic regression analyses,neither HD-WL nor HD-NBI led to a significant difference in overall adenoma detection compared to SD-WL(HD-WL:OR 0.91,P=0.69;HD-NBI:OR 1.15,P=0.80).Conclusion HD-NBI and HD-WL are comparable to SD-WL for overall adenoma detection among Chinese outpatients.It can be concluded that HD-NBI or HD-WL is not superior to SD-WL,but more effective instruction may be needed to guide the selection of different endoscopic methods in the future.Our study’s conclusions may aid in the efficient allocation and utilization of limited colonoscopy resources,especially advanced imaging technologies. 展开更多
关键词 standard-definition white-light endoscopy high-definition white-light endoscopy narrow-band imaging colonoscopy colorectal cancer screening adenoma detection rate
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Software-aided efficient identification of the components of compound formulae and their metabolites in rats by UHPLC/IM-QTOF-MS and an in-house high-definition MS^(2) library: Sishen formula as a case
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作者 Lili Hong Wei Wang +9 位作者 Shiyu Wang Wandi Hu Yuyang Sha Xiaoyan Xu Xiaoying Wang Kefeng Li Hongda Wang Xiumei Gao De-an Guo Wenzhi Yang 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2024年第10期1484-1495,共12页
Identifying the compound formulae-related xenobiotics in bio-samples is full of challenges.Conventional strategies always exhibit the insufficiencies in overall coverage,analytical efficiency,and degree of automation,... Identifying the compound formulae-related xenobiotics in bio-samples is full of challenges.Conventional strategies always exhibit the insufficiencies in overall coverage,analytical efficiency,and degree of automation,and the results highly rely on the personal knowledge and experience.The goal of this work was to establish a software-aided approach,by integrating ultra-high performance liquid chromatography/ion-mobility quadrupole time-of-flight mass spectrometry(UHPLC/IM-QTOF-MS)and in-house high-definition MS^(2) library,to enhance the identification of prototypes and metabolites of the compound formulae in vivo,taking Sishen formula(SSF)as a template.Seven different MS2 acquisition methods were compared,which demonstrated the potency of a hybrid scan approach(namely high-definition data-independent/data-dependent acquisition(HDDIDDA))in the identification precision,MS1 coverage,and MS^(2) spectra quality.The HDDIDDA data for 55 reference compounds,four component drugs,and SSF,together with the rat bio-samples(e.g.,plasma,urine,feces,liver,and kidney),were acquired.Based on the UNIFI™platform(Waters),the efficient data processing workflows were established by combining mass defect filtering(MDF)-induced classification,diagnostic product ions(DPIs),and neutral loss filtering(NLF)-dominated structural confirmation.The high-definition MS^(2) spectral libraries,dubbed in vitro-SSF and in vivo-SSF,were elaborated,enabling the efficient and automatic identification of SSF-associated xenobiotics in diverse rat bio-samples.Consequently,118 prototypes and 206 metabolites of SSF were identified,with the identification rate reaching 80.51%and 79.61%,respectively.The metabolic pathways mainly involved the oxidation,reduction,hydrolysis,sulfation,methylation,demethylation,acetylation,glucuronidation,and the combined reactions.Conclusively,the proposed strategy can drive the identification of compound formulae-related xenobiotics in vivo in an intelligent manner. 展开更多
关键词 Ultra-high performance liquid chromatography/ion-mobility quadrupole time-of-flight mass spectrometry(UHPLC/IM-QTOF-MS) Hybrid scan high-definition MS^(2)spectral library Sishen formula
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汉氏葡糖醋杆菌HDM1-3中第二信使c-di-GMP相关结构域蛋白质的生物信息学分析
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作者 杨承 庄梓涵 +3 位作者 吴昊基 陈炫 雷虹 李元敬 《黑龙江大学自然科学学报》 2025年第1期81-89,共9页
为探究汉氏葡糖醋杆菌(Komagataeibacter hansenii,K.hansenii)HDM1-3所包含的GGDEF结构域对合成环二鸟苷酸(cyclic diguanylate monophosphate,c-di-GMP)的作用,挖掘c-di-GMP对该菌种产细菌纤维素(Bacterial cellulose,BC)的作用,采用... 为探究汉氏葡糖醋杆菌(Komagataeibacter hansenii,K.hansenii)HDM1-3所包含的GGDEF结构域对合成环二鸟苷酸(cyclic diguanylate monophosphate,c-di-GMP)的作用,挖掘c-di-GMP对该菌种产细菌纤维素(Bacterial cellulose,BC)的作用,采用生物信息学方法系统分析了K.hansenii HDM1-3菌株的基因组,发现K.hansenii HDM1-3菌株中共有8个基因含有GGDEF-EAL结构域。利用Hmmer软件、Pfam数据库、SMART(Simple Modular Architecture Research Tool)平台、MEME Suite平台、MEGA11(Molecular Evolutionary Genetics Analysis 11)软件、ClustalW平台、Swiss-model平台、NCBI(National Center for Biotechnology Information)平台、InterPro平台及BLAST(Basic Local Alignment Search Tool)平台对该菌株所含有GGDEF-EAL结构域的功能进行了分析,并深入探究了该结构域对细菌纤维素产量的影响。功能预测分析表明,K.hansenii HDM1-3中含有GGDEF-EAL结构域的8个基因均参与c-di-GMP的合成、降解及信号传导。 展开更多
关键词 汉氏葡糖醋杆菌hdm1-3 C-DI-GMP 生物信息学 GGDEF-EAL结构域
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基于UPLC-HDMS技术的生脉散血清药物化学初步研究 被引量:25
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作者 吴芳芳 王喜军 +4 位作者 韩莹 魏文峰 吴修红 孙文军 孙晖 《世界科学技术-中医药现代化》 2010年第4期657-665,共9页
目的:分析鉴定口服生脉散后大鼠血中移行成分,揭示生脉散的药效物质基础。方法:应用UPLC-HDMS技术建立生脉散以及大鼠口服生脉散后含药血清样品的分析方法,通过对比空白血清、含药血清的成分异同,确定大鼠口服生脉散后的血中移行成分,... 目的:分析鉴定口服生脉散后大鼠血中移行成分,揭示生脉散的药效物质基础。方法:应用UPLC-HDMS技术建立生脉散以及大鼠口服生脉散后含药血清样品的分析方法,通过对比空白血清、含药血清的成分异同,确定大鼠口服生脉散后的血中移行成分,并利用质谱数据表征血中移行成分的结构。结果:大鼠口服生脉散后(12g·kg-1)在血中分析鉴定了18个血中移行成分,其中5个为新产生的代谢产物,13个成分为生脉散的原型成分。结论:血中移行成分及代谢产物可能为生脉散的体内直接作用物质,对其进行深入的研究将有助于阐明生脉散的有效成分及作用机理。 展开更多
关键词 生脉散 血清药物化学 血中移行成分 UPLC—hdms
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UPLC-HDMS法测定大鼠灌胃栀子柏皮片后小檗碱与栀子苷的药代动力学
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作者 徐艳丽 蒲艳春 +4 位作者 祝丽娣 刘天娇 孙向菊 刘冬敏 刘天富 《现代生物医学进展》 CAS 2019年第5期834-837,共4页
目的:通过UPLC-HDMS法测定小檗碱和栀子苷在大鼠灌胃栀子柏皮片后的药代动力学。方法:用固相萃取柱法处理血浆样本后,以正离子扫描方式,采用萃取离子监测(EIC)方式进行检测,采用Winnolin软件计算药物动力学参数。结果:小檗碱的达峰时间... 目的:通过UPLC-HDMS法测定小檗碱和栀子苷在大鼠灌胃栀子柏皮片后的药代动力学。方法:用固相萃取柱法处理血浆样本后,以正离子扫描方式,采用萃取离子监测(EIC)方式进行检测,采用Winnolin软件计算药物动力学参数。结果:小檗碱的达峰时间为1.89±0.56 h,其AUC值为793±87 ng/mL/h,药代参数显示该成分在体内浓度较低,消除较快;栀子苷的达峰时间为2.09±0.88 h,其AUC值为4687±345 ng/mL/h,药代参数显示该成分在体内表观分布容积较大,消除也较快。结论:利用高灵敏度的液质联用仪器在血中成功检测到了小檗碱及栀子苷,提示小檗碱及栀子苷为栀子柏皮片体内潜在药效作用物质。 展开更多
关键词 栀子柏皮片 小檗碱 栀子苷 UPLC/hdms
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甜瓜HDM基因家族鉴定及特性分析 被引量:2
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作者 张治 张婧 +2 位作者 张瑾 哈斯阿古拉 郝金凤 《遗传》 CAS CSCD 北大核心 2024年第2期168-180,共13页
组蛋白去甲基化酶(histone demethylase,HDM)是生物生长过程中的重要调节因子之一,在植物的生长发育和环境适应过程中发挥着重要的调控作用。本研究通过生物信息学方法对甜瓜(Cucumis melo L.)HDM基因家族进行了鉴定,并通过转录组数据... 组蛋白去甲基化酶(histone demethylase,HDM)是生物生长过程中的重要调节因子之一,在植物的生长发育和环境适应过程中发挥着重要的调控作用。本研究通过生物信息学方法对甜瓜(Cucumis melo L.)HDM基因家族进行了鉴定,并通过转录组数据检测了其成员在甜瓜组织的表达特性。结果显示,在甜瓜基因组中鉴定得到了20个CmHDM基因,在各条染色体上非均匀分布;其成员可以分成LSD1和JmjC两大类,而JmjC类群又可划分为5个亚群,各类群成员数量不等;种内和种间的共线性关系显示,甜瓜HDM基因仅存在一对片段重复,与番茄(Solanum lycopersicum)HDM基因共线性区域较多,亲缘关系较近;各成员所含保守结构域数量不等,且外显子和内含子在数量上有所差异;在启动子区域存在多种与激素和环境信号响应的顺式作用元件,表达特性分析显示各基因成员在甜瓜雄花、雌花、根、茎、叶、子房和成熟果实中均存在不同程度的表达。这些结果将有助于更好地理解HDM基因的潜在功能以及它们在参与调节甜瓜生长发育和环境自适中可能发挥的作用。 展开更多
关键词 甜瓜 hdm家族 基因鉴定 表达分析
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High-definition colonoscopy with i-Scan:Better diagnosis for small polyps and flat adenomas 被引量:12
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作者 Pier Alberto Testoni Chiara Notaristefano +2 位作者 Cristian Vailati Milena Di Leo Edi Viale 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第37期5231-5239,共9页
AIM: To investigate if high-definition (HD) colonoscope with i-Scan gave a higher detection rate of mucosal le- sions vs standard white-light instruments. METHODS: Data were collected from the computer- ized datab... AIM: To investigate if high-definition (HD) colonoscope with i-Scan gave a higher detection rate of mucosal le- sions vs standard white-light instruments. METHODS: Data were collected from the computer- ized database of the endoscopy unit of our tertiary referral center. We retrospectively analyzed 1101 con- secutive colonoscopies that were performed over 1 year with standard white-light (n = 849) or HD+ with i-Scan (n = 252) instruments by four endoscopists, in an outpatient setting. Colonoscopy records included patients' main details and family history for colorectal cancer, indication for colonoscopy (screening, diagnos- tic or surveillance), type of instrument used (standard white-light or HD+ plus i-Scan), name of endoscopist and bowel preparation. Records for each procedure included whether the cecum was reached or not and the reason for failure, complications during or imme- diately after the procedure, and number, size, location and characteristics of the lesions. Polyps or protruding lesions were defined as sessile or pedunculated, and nonprotruding lesions were defined according to Paris classification. For each lesion, histological diagnosis was recorded. RESULTS: Eight hundred and forty-nine colonosco- pies were carried with the standard white-light video colonoscope and 252 with the HD+ plus i-Scan video colonoscope, The four endoscopists did 264, 300, 276 and 261 procedures, respectively; 21.6%, 24.0%, 21.7% and 24.1% of them with the HD+ plus i-Scan technique. There were no significant differences be- tween the four endoscopists in either the number of procedures done or the proportions of each imaging technique used. Both techniques detected one or more mucosal lesions in 522/1101 procedures (47.4%). The overall number of lesions recognized was 1266; 645 in the right colon and 621 in the left. A significantly higher number of colonoscopies recognized lesions in the HD+ plus i-Scan mode (171/252 = 67.9%) than with the standard white-light technique (408/849 = 48.1%) (P 〈 0.0001). HD+ with i-Scan colonoscopies identified more lesions than standard white-light imag- ing (459/252 and 807/849, P 〈 0.0001), in the right or left colon (mean :1: SD, 1.62±1.36 vs 1.33±0.73, P 〈 0.003 and 1.55±0.98 vs 1.17±0.93, P = 0.033), more lesions 〈 10 mm (P 〈 0.0001) or nonprotruding (P 〈 0.022), and flat polyps (P = 0.04). The cumulative mean number of lesions per procedure detected by the four endoscopists was significantly higher with HD+ with i-Scan than with standard white-light imaging (1.82 ± 2.89 vs 0.95± 1.35, P 〈 0.0001). CONCLUSION: HD imaging with i-Scan during the withdrawal phase of colonoscopy significantly increased the detection of colonic mucosal lesions, particularly small and nonprotruding polyps. 展开更多
关键词 COLONOSCOPY high-definition with i-Scancolonoscopy White-light colonoscopy Colonic polyps Nonprotruding lesions Adenoma detection rate With-drawal time Surface enhancement Contrast enhance-ment Tone enhancement
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Wavelet Transform-Based High-Definition Map Construction From a Panoramic Camera 被引量:1
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作者 ZHUANG Hanyang ZHOU Xuejun +1 位作者 WANG Chunxiang QIAN Yuhan 《Journal of Shanghai Jiaotong university(Science)》 EI 2021年第5期569-576,共8页
High-definition(HD)maps are key components that provide rich topologic and semantic information for decision-making in vehicle autonomous driving systems.A complete ground orthophoto is usually used as the base image ... High-definition(HD)maps are key components that provide rich topologic and semantic information for decision-making in vehicle autonomous driving systems.A complete ground orthophoto is usually used as the base image to construct the HD map.The ground orthophoto is obtained through inverse perspective transformation and image mosaicing.During the image mosaicing,multiple consecutive orthophotos are stitched together using pose information and image registration.In this study,wavelet transform is introduced to the image mosaicing process to alleviate the information loss caused by image overlapping.In the orthophoto wavelet transform,high-frequency and low-frequency components are fused using different strategies to form a complete base image with clearer local details.Experimental results show that the accuracy of the orthophotos generated using this method is improved. 展开更多
关键词 high-definition(HD)map wavelet transform image registration PANORAMA
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SYNAPT G2 HDMS高清质谱性能特点及应用简介 被引量:1
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作者 王灼维 贾伟 Swee Lee Yap 《现代仪器》 2010年第5期20-24,共5页
SYNAPT^(TM) G2 HDMS质谱分析系统是沃特世公司第二代基于高效离子淌度(ion mobility spectrometry,IMS,也称离子迁移)技术的高性能四极杆-飞行时间质谱仪。通过两种技术的结合与升级,SYNAPT G2 HDMS不仅能按离子的质荷比分离检测,还可... SYNAPT^(TM) G2 HDMS质谱分析系统是沃特世公司第二代基于高效离子淌度(ion mobility spectrometry,IMS,也称离子迁移)技术的高性能四极杆-飞行时间质谱仪。通过两种技术的结合与升级,SYNAPT G2 HDMS不仅能按离子的质荷比分离检测,还可根据离子的大小、形状等将其分离。因此,此质谱系统不但可以提高传统质谱数据的准确性,更可以提供样品形态信息,从而得到样品活性、结构状态等信息。其在蛋白组学、代谢组学、糖及脂类分析、医学标志物发掘、药物开发与质控、食品安全检测、环境安全检测等方面有着广泛的应用空间,并已经表现出良好的性能优势,成为各领域工作者的有力的分析工具。本文简要介绍该系统的性能特点及应用。 展开更多
关键词 SYNAPT G2 hdms 离子淌度 蛋白质组学 代谢组学
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Optical diagnosis of colorectal polyps using high-definition i-scan: An educational experience
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作者 Mariёlle WE Bouwens Rogier de Ridder +4 位作者 Ad AM Masclee Ann Driessen Robert G Riedl Bjorn Winkens Silvia Sanduleanu 《World Journal of Gastroenterology》 SCIE CAS 2013年第27期4334-4343,共10页
AIM:To examine performances regarding prediction of polyp histology using high-definition (HD) i-scan in a group of endoscopists with varying levels of experience. METHODS:We used a digital library of HD i-scan still ... AIM:To examine performances regarding prediction of polyp histology using high-definition (HD) i-scan in a group of endoscopists with varying levels of experience. METHODS:We used a digital library of HD i-scan still images, comprising twin pictures (surface enhancement and tone enhancement), collected at our university hospital. We defined endoscopic features of adenomatous and non-adenomatous polyps, according to the following parameters:color, surface pattern and vascular pattern. We familiarized the participating endoscopists on optical diagnosis of colorectal polyps using a 20-min didactic training session. All endoscopists were asked to evaluate an image set of 50 colorectal polyps with regard to polyp histology. We classified the diagnoses into high confidence (i.e., cases in which the endoscopist could assign a diagnosis with certainty) and low confidence diagnoses (i.e., cases in which the endoscopist preferred to send the polyp for formal histology). Mean sensitivity, specificity and accuracy per endoscopist/image were computed and differences between groups tested using independent-samples t tests. High vs low confidence diagnoses were compared using the pairedsamples t test. RESULTS:Eleven endoscopists without previous experience on optical diagnosis evaluated a total of 550 images (396 adenomatous, 154 non-adenomatous). Mean sensitivity, specificity and accuracy for diagnosing adenomas were 79.3%, 85.7% and 81.1%, respectively. No significant differences were found between gastroenterologists and trainees regarding performances of optical diagnosis (mean accuracy 78.0%vs 82.9%,P = 0.098). Diminutive lesions were predicted with a lower mean accuracy as compared to non-diminutive lesions (74.2% vs 93.1%, P = 0.008). A total of 446 (81.1%) diagnoses were made with high confidence. High confidence diagnoses corresponded to a significantly higher mean accuracy than low confidence diagnoses (84.0% vs 64.3%, P = 0.008). A total of 319 (58.0%) images were evaluated as having excellent quality. Considering excellent quality images in conjunction with high confidence diagnosis, overall accuracy increased to 92.8%. CONCLUSION:After a single training session, endoscopists with varying levels of experience can already provide optical diagnosis with an accuracy of 84.0%. 展开更多
关键词 COLONOSCOPY high-definition i-scan Optical diagnosis Colorectal POLYPS Training
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A Reliable Adaptive High-Definition Transmission System for Distance Learning
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作者 徐奇 李鹤廷 《Journal of Shanghai Jiaotong university(Science)》 EI 2007年第6期740-743,共4页
There is an increasing demand for supporting high-quality real-time audiovisual services for the next generation wired and wireless networks. However, due to variety of bandwidths of different networks, it is a great ... There is an increasing demand for supporting high-quality real-time audiovisual services for the next generation wired and wireless networks. However, due to variety of bandwidths of different networks, it is a great challenge for deployment. In this paper, a novel high-definition (HD) video transmission system was proposed which depends upon reliable compound multicast protocols and QoS control over the various kinds of networks. This system detects client's network condition and assigns it to a proper proxy. Each proxy is capable of detecting network parameters and adaptively tuning such transport parameters as bit rate, video resolution, frame rate and QoS mechanisms to this condition. It also provides FEC error recovery under consideration of characteristics of MPEG4 video codec. Our simulation demonstrates that different network clients such as ADSL, CERNET, and CERNET2 can receive more video reliability with less delay. 展开更多
关键词 ADAPTIVE high-definition(HD) transmission system distance learning RELIABLE compound MULTICAST protocol (RCMP)
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汉氏葡糖醋杆菌Komagataeibacter hansenii HDM1-3不同发酵时间的转录组测序分析
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作者 邱永杰 李元敬 +2 位作者 郭丹 雷虹 李文辉 《黑龙江大学自然科学学报》 CAS 2024年第3期321-331,共11页
为了探究汉氏葡糖醋杆菌HDM1-3(Komagataeibacter hansenii HDM1-3)在不同发酵时间的差异基因表达水平,挖掘差异表达基因在细菌纤维素(Bacterial cellulose,BC)生物合成代谢相关基因中的功能,利用Illumina HiSeq平台对发酵前期(18 h)和... 为了探究汉氏葡糖醋杆菌HDM1-3(Komagataeibacter hansenii HDM1-3)在不同发酵时间的差异基因表达水平,挖掘差异表达基因在细菌纤维素(Bacterial cellulose,BC)生物合成代谢相关基因中的功能,利用Illumina HiSeq平台对发酵前期(18 h)和发酵后期(36 h)的Komagataeibacter hansenii HDM1-3菌株进行转录组测序分析,通过荧光定量PCR验证转录组测序结果的准确性,通过GO富集和KEGG富集分析差异表达基因。转录组测序共获得3154个CDS序列,其中57个未被注释,注释率达98.22%。差异表达基因分析表明,菌株HDM1-3发酵36 h相比于发酵18 h,共有684个显著性差异表达基因,其中上调基因369个(53.9%),下调基因315个(46.1%)。GO功能富集分析结果表明,发酵后期碳水化合物代谢相关功能基因下调,而细胞膜相关功能基因上调。KEGG富集分析结果表明,主要的能量代谢途径,包括糖酵解途径、磷酸戊糖途径和柠檬酸循环都显著下调,两个BC合成酶基因显著上调。这些结果为进一步分析HDM1-3菌株BC合成相关基因、提高BC产量的研究提供理论参考。 展开更多
关键词 汉氏葡糖醋杆菌hdm1-3 差异表达基因 荧光定量PCR 细菌纤维素
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Waters Synapt HDMS质谱分析系统
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《环境化学》 CAS CSCD 北大核心 2007年第2期F0002-F0002,共1页
沃特世公司(NYSE:WAT)生产的Waters Synapt HDMS质谱分析系统在分析化学和应用谱学匹兹堡展会(Pittcon)上荣获2007年度Pittcon最佳新产品金奖.这是继Waters ACQUITY UPLC系统于2004年喜获金奖以来,Waters Synapt”HDMSTM质谱分... 沃特世公司(NYSE:WAT)生产的Waters Synapt HDMS质谱分析系统在分析化学和应用谱学匹兹堡展会(Pittcon)上荣获2007年度Pittcon最佳新产品金奖.这是继Waters ACQUITY UPLC系统于2004年喜获金奖以来,Waters Synapt”HDMSTM质谱分析系统(Waters Synapt High Definition MS(HDMS)System)又一次赢得了这一最高奖项. 展开更多
关键词 PLC系统 质谱分析 分析化学 匹兹堡 谱学 WATERS Synapt hdms质谱分析系统
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Application of Visual High-definition Rigid Bronchoscope in Removal of Complicated Respiratory Foreign Body
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作者 HE Meng CHENG Yanbin BAO Yan 《外文科技期刊数据库(文摘版)医药卫生》 2021年第12期997-1000,共6页
Objective: to explore and analyze the effect of applying visual high-definition rigid bronchoscopy in the removal of complicated respiratory foreign body. Methods: from September 2017 to June 2020, 97 patients with co... Objective: to explore and analyze the effect of applying visual high-definition rigid bronchoscopy in the removal of complicated respiratory foreign body. Methods: from September 2017 to June 2020, 97 patients with complex foreign body in respiratory tract with valid data registered in the database of our hospital were selected as the research subjects. The basic data of the patients were statistically analyzed. All the patients in this study had complicated foreign body obstruction in respiratory tract. After admission, the medical staff gave the patients visual high-definition rigid bronchoscopy for auxiliary treatment, and the final treatment effect was evaluated and analyzed. Results: in this study, 97 patients with complex foreign body in respiratory tract were improved after treatment, with a total effective rate of 100%. In this study, there were 2 patients with respiratory tract injury during surgery, with an incidence rate of 2.06%. Conclusion: in the removal of complicated respiratory foreign body, the choice of visual high-definition rigid bronchoscope for treatment can help medical staff to identify respiratory foreign body and promote the outcome of the patient's condition, which has good promotion value and is worthy of further application. 展开更多
关键词 visual high-definition rigid bronchoscopy removal of complex respiratory foreign body treatment op
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HDM钢热处理工艺研究 被引量:3
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作者 彭程 刘树勋 +1 位作者 李培杰 曾大本 《金属热处理》 CAS CSCD 北大核心 2002年第5期3-6,共4页
通过选择不同固溶、时效温度组合 ,对新型镁合金压铸用模具钢 (HDM钢 )的组织及性能和热处理工艺之间的关系进行了详细地探讨 ,确定出HDM钢最佳固溶时效热处理工艺为 (110 0± 10 )℃× 1h +(70 0 +10 )℃× 2h。
关键词 hdm 热处理 热作模具钢 压铸
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HDM通过肺泡巨噬细胞TLR4诱导哮喘小鼠气道炎症的机制研究 被引量:2
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作者 郝俊青 李鸿佳 +5 位作者 李艳丽 王荣 姜虹 王旭平 毕文祥 董亮 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2009年第12期1095-1097,共3页
目的:研究HDM通过肺泡巨噬细胞(AM)Toll样受体4(TLR4)的高表达及诱导AM的活化,探讨其对哮喘小鼠气道炎症的影响。方法:BALB/c小鼠随机分为哮喘模型组(OVA)A,HDM处理组(HDM+OVA)B,对照组(生理盐水)C。用卵白蛋白(OVA)致敏与激发建立小鼠... 目的:研究HDM通过肺泡巨噬细胞(AM)Toll样受体4(TLR4)的高表达及诱导AM的活化,探讨其对哮喘小鼠气道炎症的影响。方法:BALB/c小鼠随机分为哮喘模型组(OVA)A,HDM处理组(HDM+OVA)B,对照组(生理盐水)C。用卵白蛋白(OVA)致敏与激发建立小鼠哮喘模型;HE染色观察小鼠气道及肺组织病理变化;光学显微镜下观察小鼠支气管肺泡灌洗液(BALF)中细胞分类及计数;酶联免疫吸附试验(ELISA)检测小鼠BALF上清中IL-4、IL-5、IL-13和IFN-γ的含量,实时定量PCR法测定AM的TLR4的表达,流式细胞技术(FCM)检测AM的CD80、CD86的表达。结果:与A组相比,B组BALF上清中IL-4、IL-5和IL-13的水平显著增高(P<0.05),而IFN-γ差异无统计学意义(P>0.05),与A组相比,AM的TLR4mRNA表达明显增高(P<0.05),CD80的表达差异无统计学意义,CD86的表达水平显著增高,差异有统计学意义(P<0.05)。结论:HDM通过AM的TLR4的高表达诱导AM的活化,加重哮喘的气道炎症。 展开更多
关键词 肺泡巨噬细胞 hdm TOLL样受体4 支气管哮喘
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HDM性哮喘疫苗研究进展 被引量:2
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作者 范怡敏 程训佳 《现代免疫学》 CAS CSCD 北大核心 2004年第5期437-438,F003,共3页
HDM能引起多种变应性疾病 ,已发现的HDM变应原有 19类 ,其中第 1类和第 2类最为重要。变应原来源的多肽或者编码HDM变应原多肽的DNA可以用于制备HDM性哮喘疫苗。应用一些可生物降解的微球包装质粒DNA ,或者同时运用CpG以及编码Th1细胞... HDM能引起多种变应性疾病 ,已发现的HDM变应原有 19类 ,其中第 1类和第 2类最为重要。变应原来源的多肽或者编码HDM变应原多肽的DNA可以用于制备HDM性哮喘疫苗。应用一些可生物降解的微球包装质粒DNA ,或者同时运用CpG以及编码Th1细胞因子的DNA等能加强预防接种疫苗引起的免疫反应 ,起到佐剂的作用 ;以BCG等与HDM变应原进行联合免疫亦能增强其效应。随着研究的深入 。 展开更多
关键词 hdm 哮喘 疫苗
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HDM2和p53蛋白的相互作用及其抑制剂的研究 被引量:5
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作者 李文东 刘芝华 《国外医学(肿瘤学分册)》 2002年第4期255-258,共4页
HDM 2和 p5 3蛋白之间存在一个自动调节的负反馈环。通过抑制二者的相互作用可治疗肿瘤。近年来 ,寻找抑制这种相互作用的药物已成为肿瘤治疗的研究热点。
关键词 hdm2 P53蛋白 肿瘤 抑制剂
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Hdm2剪接体促进结直肠癌细胞增殖的分子机制 被引量:1
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作者 周锋 沙德胜 刘红 《中外医疗》 2018年第30期1-4,共4页
目的探讨Hdm2剪接体促进结直肠癌细胞增殖的分子机制。方法该研究内容起止时间:2015年1月—2017年12月。构建Hdm2剪接体的稳转细胞株后进行CCK-8的检测,并利用western-blot相关下游蛋白的检测。结果Hdm2剪接体与对照组相比可以促进结肠... 目的探讨Hdm2剪接体促进结直肠癌细胞增殖的分子机制。方法该研究内容起止时间:2015年1月—2017年12月。构建Hdm2剪接体的稳转细胞株后进行CCK-8的检测,并利用western-blot相关下游蛋白的检测。结果Hdm2剪接体与对照组相比可以促进结肠癌细胞的增殖(P<0.05),p53和p21的表达明显下降(P<0.05),同时上调pp38和p38的表达(P<0.05)。结论 Hdm2剪切体与结肠癌细胞的增殖相关,其通过下调抑癌基因p53,p21的表达,激活p-p38和p38的表达促进结直肠癌的发生和发展。 展开更多
关键词 结直肠癌 hdm2剪切体 MRNA P53
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