The Arabidopsis thaliana RPD3-type histone deacetylases have been known to form conserved SIN3-type histone deacetylase complexes,but whether they form other types of complexes is unknown.Here,we perform affinity puri...The Arabidopsis thaliana RPD3-type histone deacetylases have been known to form conserved SIN3-type histone deacetylase complexes,but whether they form other types of complexes is unknown.Here,we perform affinity purification followed by mass spectrometry and demonstrate that the Arabidopsis RPD3-type histone deacetylases HDA6 and HDA19 interact with several previously uncharacterized proteins,thereby forming three types of plant-specific histone deacetylase complexes,which we named SANT,ESANT,and ARID.RNA-seq indicates that the newly identified components function together with HDA6 and HDA19 and coregulate the expression of a number of genes.HDA6 and HDA19 were previously thought to repress gene transcription by histone deacetylation.We find that the histone deacetylase complexes can repress gene expression via both histone deacetylation-dependent and-independent mechanisms.In the mutants of histone deacetylase complexes,the expression of a number of stressinduced genes is up-regulated,and several mutants of the histone deacetylase complexes show severe retardation in growth.Considering that growth retardation is thought to be a trade-off for an increase in stress tolerance,we infer that the histone deacetylase complexes identified in this study prevent overexpression of stress-induced genes and thereby ensure normal growth of plants under nonstress conditions.展开更多
Anthocyanins are plant pigments that play diverse roles in plant growth,adaptation,and stress tolerance.Anthocyanin biosynthesis is tightly regulated,but the underlying regulatory mechanisms remain unclear.Here,we ide...Anthocyanins are plant pigments that play diverse roles in plant growth,adaptation,and stress tolerance.Anthocyanin biosynthesis is tightly regulated,but the underlying regulatory mechanisms remain unclear.Here,we identify a regulatory module composed of the DNA-binding protein VAL1(VIVIPAROUS1/ABI3LIKE 1)and a SIN3(SWI-INDEPENDENT 3)-like histone deacetylase complex that dynamically regulates anthocyanin biosynthesis in Arabidopsis thaliana.Under normal growth conditions,VAL1 recruits the SNL(SIN3-Like)-HDA19(HISTONE DEACETYLASE 19)complex(SNL-HDA19c)to the PRODUCTION OF ANTHOCYANIN PIGMENT 1(PAP1)locus for histone deacetylation.Moreover,the negative regulators of jasmonic acid(JA)signaling,JASMONATE-ZIM DOMAIN(JAZ)proteins,interact with VAL1 and further stabilize the binding of VAL1 and SNL-HDA19c to PAP1 chromatin.These molecular interactions transcriptionally repress PAP1 and inhibit anthocyanin biosynthesis.Upon JA accumulation,JAZs are degraded,resulting in the release of both VAL1 and SNL-HDA19c from the PAP1 chromatin.This release leads to an immediate increase in histone acetylation,promoting transcriptional activation of PAP1 and anthocyanin production.These findings elucidate a regulatory module(VAL1-JAZ-SNL-HDA19c)that represses anthocyanin biosynthesis under normal growth conditions and further reveal how the stress hormone JA rapidly induces anthocyanin production,enabling plants to adapt to their growth conditions.展开更多
It has been almost 20 years since the first report of a WRKY transcription factor, SPF1, from sweet potato. Great progress has been made since then in establishing the diverse biological roles of WRKY transcription fa...It has been almost 20 years since the first report of a WRKY transcription factor, SPF1, from sweet potato. Great progress has been made since then in establishing the diverse biological roles of WRKY transcription factors in plant growth, development, and responses to biotic and abiotic stress. Despite the functional diversity, almost all ana-lyzed WRKY proteins recognize the TrGACC/T W-box sequences and, therefore, mechanisms other than mere recognition of the core W-box promoter elements are necessary to achieve the regulatory specificity of WRKY transcription factors. Research over the past several years has revealed that WRKY transcription factors physically interact with a wide range of proteins with roles in signaling, transcription, and chromatin remodeling. Studies of WRKY-interacting proteins have provided important insights into the regulation and mode of action of members of the important family of transcrip-tion factors. It has also emerged that the slightly varied WRKY domains and other protein motifs conserved within each of the seven WRKY subfamilies participate in protein-protein interactions and mediate complex functional interactions between WRKY proteins and between WRKY and other regulatory proteins in the modulation of important biologi- cal processes. In this review, we summarize studies of protein-protein interactions for WRKY transcription factors and discuss how the interacting partners contribute, at different levels, to the establishment of the complex regulatory and functional network of WRKY transcription factors.展开更多
基金supported by the National Natural Science Foundation of China(32025003)by the National Key Research and Development Program of China(2016YFA0500801)from the Chinese Ministry of Science and Technology。
文摘The Arabidopsis thaliana RPD3-type histone deacetylases have been known to form conserved SIN3-type histone deacetylase complexes,but whether they form other types of complexes is unknown.Here,we perform affinity purification followed by mass spectrometry and demonstrate that the Arabidopsis RPD3-type histone deacetylases HDA6 and HDA19 interact with several previously uncharacterized proteins,thereby forming three types of plant-specific histone deacetylase complexes,which we named SANT,ESANT,and ARID.RNA-seq indicates that the newly identified components function together with HDA6 and HDA19 and coregulate the expression of a number of genes.HDA6 and HDA19 were previously thought to repress gene transcription by histone deacetylation.We find that the histone deacetylase complexes can repress gene expression via both histone deacetylation-dependent and-independent mechanisms.In the mutants of histone deacetylase complexes,the expression of a number of stressinduced genes is up-regulated,and several mutants of the histone deacetylase complexes show severe retardation in growth.Considering that growth retardation is thought to be a trade-off for an increase in stress tolerance,we infer that the histone deacetylase complexes identified in this study prevent overexpression of stress-induced genes and thereby ensure normal growth of plants under nonstress conditions.
基金supported in part by the National Key Research and Development Program of China(grant no.2024YFF1000301)National Natural Science Foundation of China(grant no.32330007)Peking-Tsinghua Center for Life Sciences,and the Chinese Academy of Sciences.
文摘Anthocyanins are plant pigments that play diverse roles in plant growth,adaptation,and stress tolerance.Anthocyanin biosynthesis is tightly regulated,but the underlying regulatory mechanisms remain unclear.Here,we identify a regulatory module composed of the DNA-binding protein VAL1(VIVIPAROUS1/ABI3LIKE 1)and a SIN3(SWI-INDEPENDENT 3)-like histone deacetylase complex that dynamically regulates anthocyanin biosynthesis in Arabidopsis thaliana.Under normal growth conditions,VAL1 recruits the SNL(SIN3-Like)-HDA19(HISTONE DEACETYLASE 19)complex(SNL-HDA19c)to the PRODUCTION OF ANTHOCYANIN PIGMENT 1(PAP1)locus for histone deacetylation.Moreover,the negative regulators of jasmonic acid(JA)signaling,JASMONATE-ZIM DOMAIN(JAZ)proteins,interact with VAL1 and further stabilize the binding of VAL1 and SNL-HDA19c to PAP1 chromatin.These molecular interactions transcriptionally repress PAP1 and inhibit anthocyanin biosynthesis.Upon JA accumulation,JAZs are degraded,resulting in the release of both VAL1 and SNL-HDA19c from the PAP1 chromatin.This release leads to an immediate increase in histone acetylation,promoting transcriptional activation of PAP1 and anthocyanin production.These findings elucidate a regulatory module(VAL1-JAZ-SNL-HDA19c)that represses anthocyanin biosynthesis under normal growth conditions and further reveal how the stress hormone JA rapidly induces anthocyanin production,enabling plants to adapt to their growth conditions.
文摘It has been almost 20 years since the first report of a WRKY transcription factor, SPF1, from sweet potato. Great progress has been made since then in establishing the diverse biological roles of WRKY transcription factors in plant growth, development, and responses to biotic and abiotic stress. Despite the functional diversity, almost all ana-lyzed WRKY proteins recognize the TrGACC/T W-box sequences and, therefore, mechanisms other than mere recognition of the core W-box promoter elements are necessary to achieve the regulatory specificity of WRKY transcription factors. Research over the past several years has revealed that WRKY transcription factors physically interact with a wide range of proteins with roles in signaling, transcription, and chromatin remodeling. Studies of WRKY-interacting proteins have provided important insights into the regulation and mode of action of members of the important family of transcrip-tion factors. It has also emerged that the slightly varied WRKY domains and other protein motifs conserved within each of the seven WRKY subfamilies participate in protein-protein interactions and mediate complex functional interactions between WRKY proteins and between WRKY and other regulatory proteins in the modulation of important biologi- cal processes. In this review, we summarize studies of protein-protein interactions for WRKY transcription factors and discuss how the interacting partners contribute, at different levels, to the establishment of the complex regulatory and functional network of WRKY transcription factors.
