Intracellular bacteria(ICB),cloaked by the protective barriers of host cells,pose a formidable challenge to selective and efficient eradication.The employment of activatable photosensitizers based antibacterial photod...Intracellular bacteria(ICB),cloaked by the protective barriers of host cells,pose a formidable challenge to selective and efficient eradication.The employment of activatable photosensitizers based antibacterial photodynamic therapy(a PDT)holds significant potential for selective imaging and photo-inactivation of ICB while minimizing side effects on normal cells.Drawing inspiration from the elevated hypochlorous acid(HClO)levels in ICB infected phagocytes,herein we firstly designed and synthesized a series of HCl Oresponsive dinuclear Ru(Ⅱ)complexes(Ru1-Ru3)to achieve such a goal.Initially,the luminescence,^(1)O_(2)generation and a PDT activity of these Ru(Ⅱ)complexes were suppressed due to the quenching effect of the azo group,but were recovered after reaction with HCl O in solutions or within ICB infected phagocytes.The detailed results revealed that Ru1 and Ru3 could not only selectively visualize ICB,but also demonstrated remarkable a PDT activity against ICB,surpassing vancomycin both in vitro and in vivo.展开更多
基金supported by National Natural Science Foundation of China(No.22371289)。
文摘Intracellular bacteria(ICB),cloaked by the protective barriers of host cells,pose a formidable challenge to selective and efficient eradication.The employment of activatable photosensitizers based antibacterial photodynamic therapy(a PDT)holds significant potential for selective imaging and photo-inactivation of ICB while minimizing side effects on normal cells.Drawing inspiration from the elevated hypochlorous acid(HClO)levels in ICB infected phagocytes,herein we firstly designed and synthesized a series of HCl Oresponsive dinuclear Ru(Ⅱ)complexes(Ru1-Ru3)to achieve such a goal.Initially,the luminescence,^(1)O_(2)generation and a PDT activity of these Ru(Ⅱ)complexes were suppressed due to the quenching effect of the azo group,but were recovered after reaction with HCl O in solutions or within ICB infected phagocytes.The detailed results revealed that Ru1 and Ru3 could not only selectively visualize ICB,but also demonstrated remarkable a PDT activity against ICB,surpassing vancomycin both in vitro and in vivo.
