目的采用腹部横切手术及肝门静脉注射法将丙型肝炎病毒(hepatitis c virus,HCV)迅速足量注射接种到树鼩体内肝脏,提高HCV对树鼩的感染率。方法采用横切打开树鼩腹腔,找到肝门静脉,进行肝门静脉注射接种HCV,手术后1周进行采血、ALT检测、...目的采用腹部横切手术及肝门静脉注射法将丙型肝炎病毒(hepatitis c virus,HCV)迅速足量注射接种到树鼩体内肝脏,提高HCV对树鼩的感染率。方法采用横切打开树鼩腹腔,找到肝门静脉,进行肝门静脉注射接种HCV,手术后1周进行采血、ALT检测、HCV病毒载量检测、肝脏病理检测及HCV-RNA抗原检测。结果横切手术及肝门静脉注射20只树鼩,手术后经护理恢复,树鼩伤口恢复良好,手术成功率及肝门静脉注射准确率均达100%,保证HCV完全快速进入树鼩肝脏。有60%(12/20)树鼩出现一过性的病毒血症或间歇性病毒血症,感染树鼩的病毒载量最高可达到1x105拷贝/mL,肝功能中ALT指标最高可达到192μ/L,肝脏活体组织病理检查发现不同程度的肝炎症状,肝组织能检测到HCV-RNA抗原。结论腹部横切具有充分暴露肝门静脉、操作方便、符合解剖生理、组织创伤较小、伤口易于愈合等优点,适用于肝门静脉注射,同时提高了感染率,该技术方法为进一步深入研究HCV感染树鼩奠定基础,对于其它相关实验动物和动物实验具有一定借鉴经验和参考价值。展开更多
The aim of this work is to analyse the global dynamics of an extended mathematical model of Hepatitis C virus (HCV) infection in vivo with cellular proliferation, spontaneous cure and hepatocyte homeostasis. We firstl...The aim of this work is to analyse the global dynamics of an extended mathematical model of Hepatitis C virus (HCV) infection in vivo with cellular proliferation, spontaneous cure and hepatocyte homeostasis. We firstly prove the existence of local and global solutions of the model and establish some properties of this solution as positivity and asymptotic behaviour. Secondly we show, by the construction of appropriate Lyapunov functions, that the uninfected equilibrium and the unique infected equilibrium of the mathematical model of HCV are globally asymptotically stable respectively when the threshold number and when .展开更多
丙型肝炎病毒(Hepatitis C virus,HCV)属于黄病毒科肝炎病毒属的病毒,不经治疗可能发展为肝硬化和肝癌。虽然在丙型肝炎治疗上已经取得了很大进步,但是HCV疫苗仍然没有研制出来。HCV疫苗主要困难在于HCV基因遗传多样性、HCV持续性感染...丙型肝炎病毒(Hepatitis C virus,HCV)属于黄病毒科肝炎病毒属的病毒,不经治疗可能发展为肝硬化和肝癌。虽然在丙型肝炎治疗上已经取得了很大进步,但是HCV疫苗仍然没有研制出来。HCV疫苗主要困难在于HCV基因遗传多样性、HCV持续性感染的多种机制、宿主在抗病毒方面较差的免疫反应以及缺乏合适的模型系统等方面。了解固有免疫和适应性免疫应答对候选疫苗的设计也很重要。一个疫苗技术的创新应用需要生物学评价、保护性免疫评价及临床试验等各方面评估。展开更多
Background: Chronic Hepatitis C virus (HCV) is an increasing threat to international public health, as it can have severe impacts on morbidity and mortality. Burundi has done little to address the hepatitis C burden i...Background: Chronic Hepatitis C virus (HCV) is an increasing threat to international public health, as it can have severe impacts on morbidity and mortality. Burundi has done little to address the hepatitis C burden in-country. This analysis aims to address the current epidemiological landscape of HCV in Burundi and identify efforts to achieve elimination by 2030. Methods: In order to estimate the chronic HCV population through 2030, an Excel-based Markov model was created;calibrated using historical data populated from a literature review and an in-country lead expert panel. Using this model, two scenarios were developed: Base 2017, continuing the current standard of care through 2030, and Elimination, where treatment and diagnosis were increased to achieve a 90% reduction in total infections by 2030. Results: In 2017, there were estimated 392,000 viremic infections, with a viremic rate of 3.3%. Under the Base 2017 scenario, the number of viremic infections is expected to decline by less than 15% by 2030, while liver related morbidity and mortality are expected to increase, with an estimated 2100 deaths due to HCV by 2030. Under the Elimination strategy, outcomes are estimated to significantly improve, with viremic infections declining by an estimated 90% by 2030 and HCV-related mortality expected to decrease by 85%. More than 12,470 lives could be saved by achieving elimination by 2030. Conclusion: Achieving elimination of HCV in Burundi is reasonable with implementation of increased diagnosis and treatment efforts, including affordable programming to reduce liver-related morbidity and mortality.展开更多
丙型肝炎病毒(Hepatitis C virus,HCV)是一种危害人类健康的病原体,感染人体后极易导致慢性肝炎,并能引起肝纤维化或脂肪肝,可能进一步发展成为肝硬化、肝癌等终末期肝病。尽管已被发现20多年,丙肝病毒的来源以及进化途径一直没有确定...丙型肝炎病毒(Hepatitis C virus,HCV)是一种危害人类健康的病原体,感染人体后极易导致慢性肝炎,并能引起肝纤维化或脂肪肝,可能进一步发展成为肝硬化、肝癌等终末期肝病。尽管已被发现20多年,丙肝病毒的来源以及进化途径一直没有确定。与此同时,缺乏合适的动物模型严重阻碍HCV致病机理的研究。从2011年起,随着新型测序技术的应用,多种丙肝非灵长类同源病毒相继被发现,这些研究成果将在研究丙肝病毒来源、进化途径以及相关动物模型建立中起重要作用。展开更多
文摘目的采用腹部横切手术及肝门静脉注射法将丙型肝炎病毒(hepatitis c virus,HCV)迅速足量注射接种到树鼩体内肝脏,提高HCV对树鼩的感染率。方法采用横切打开树鼩腹腔,找到肝门静脉,进行肝门静脉注射接种HCV,手术后1周进行采血、ALT检测、HCV病毒载量检测、肝脏病理检测及HCV-RNA抗原检测。结果横切手术及肝门静脉注射20只树鼩,手术后经护理恢复,树鼩伤口恢复良好,手术成功率及肝门静脉注射准确率均达100%,保证HCV完全快速进入树鼩肝脏。有60%(12/20)树鼩出现一过性的病毒血症或间歇性病毒血症,感染树鼩的病毒载量最高可达到1x105拷贝/mL,肝功能中ALT指标最高可达到192μ/L,肝脏活体组织病理检查发现不同程度的肝炎症状,肝组织能检测到HCV-RNA抗原。结论腹部横切具有充分暴露肝门静脉、操作方便、符合解剖生理、组织创伤较小、伤口易于愈合等优点,适用于肝门静脉注射,同时提高了感染率,该技术方法为进一步深入研究HCV感染树鼩奠定基础,对于其它相关实验动物和动物实验具有一定借鉴经验和参考价值。
文摘The aim of this work is to analyse the global dynamics of an extended mathematical model of Hepatitis C virus (HCV) infection in vivo with cellular proliferation, spontaneous cure and hepatocyte homeostasis. We firstly prove the existence of local and global solutions of the model and establish some properties of this solution as positivity and asymptotic behaviour. Secondly we show, by the construction of appropriate Lyapunov functions, that the uninfected equilibrium and the unique infected equilibrium of the mathematical model of HCV are globally asymptotically stable respectively when the threshold number and when .
文摘丙型肝炎病毒(Hepatitis C virus,HCV)属于黄病毒科肝炎病毒属的病毒,不经治疗可能发展为肝硬化和肝癌。虽然在丙型肝炎治疗上已经取得了很大进步,但是HCV疫苗仍然没有研制出来。HCV疫苗主要困难在于HCV基因遗传多样性、HCV持续性感染的多种机制、宿主在抗病毒方面较差的免疫反应以及缺乏合适的模型系统等方面。了解固有免疫和适应性免疫应答对候选疫苗的设计也很重要。一个疫苗技术的创新应用需要生物学评价、保护性免疫评价及临床试验等各方面评估。
文摘Background: Chronic Hepatitis C virus (HCV) is an increasing threat to international public health, as it can have severe impacts on morbidity and mortality. Burundi has done little to address the hepatitis C burden in-country. This analysis aims to address the current epidemiological landscape of HCV in Burundi and identify efforts to achieve elimination by 2030. Methods: In order to estimate the chronic HCV population through 2030, an Excel-based Markov model was created;calibrated using historical data populated from a literature review and an in-country lead expert panel. Using this model, two scenarios were developed: Base 2017, continuing the current standard of care through 2030, and Elimination, where treatment and diagnosis were increased to achieve a 90% reduction in total infections by 2030. Results: In 2017, there were estimated 392,000 viremic infections, with a viremic rate of 3.3%. Under the Base 2017 scenario, the number of viremic infections is expected to decline by less than 15% by 2030, while liver related morbidity and mortality are expected to increase, with an estimated 2100 deaths due to HCV by 2030. Under the Elimination strategy, outcomes are estimated to significantly improve, with viremic infections declining by an estimated 90% by 2030 and HCV-related mortality expected to decrease by 85%. More than 12,470 lives could be saved by achieving elimination by 2030. Conclusion: Achieving elimination of HCV in Burundi is reasonable with implementation of increased diagnosis and treatment efforts, including affordable programming to reduce liver-related morbidity and mortality.
文摘丙型肝炎病毒(Hepatitis C virus,HCV)是一种危害人类健康的病原体,感染人体后极易导致慢性肝炎,并能引起肝纤维化或脂肪肝,可能进一步发展成为肝硬化、肝癌等终末期肝病。尽管已被发现20多年,丙肝病毒的来源以及进化途径一直没有确定。与此同时,缺乏合适的动物模型严重阻碍HCV致病机理的研究。从2011年起,随着新型测序技术的应用,多种丙肝非灵长类同源病毒相继被发现,这些研究成果将在研究丙肝病毒来源、进化途径以及相关动物模型建立中起重要作用。
