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盐穗木病程相关蛋白HcPR10抗血清的制备及鉴定
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作者 张花 杨涛 +1 位作者 衡友强 王艳 《广西植物》 CAS CSCD 北大核心 2020年第12期1732-1739,共8页
病程相关蛋白(PRs)在植物抗病抗逆过程中发挥重要作用。盐穗木病程相关蛋白基因HcPR10(GenBank:KF673356)来自盐穗木(Halostachys capsica)在600 mmol·L^-1NaCl胁迫下的盐抑制差减文库。为探究盐穗木病程相关蛋白HcPR10发挥生物学... 病程相关蛋白(PRs)在植物抗病抗逆过程中发挥重要作用。盐穗木病程相关蛋白基因HcPR10(GenBank:KF673356)来自盐穗木(Halostachys capsica)在600 mmol·L^-1NaCl胁迫下的盐抑制差减文库。为探究盐穗木病程相关蛋白HcPR10发挥生物学功能的机制,该研究通过体外表达和纯化HcPR10重组蛋白制备特异性的HcPR10多克隆抗体。并采用双酶切构建原核重组表达载体pET28a-HcPR10,转化至大肠杆菌(Escherichia coli)BL21诱导表达,通过正交分析优化重组蛋白可溶性诱导表达的条件,利用Ni-NTA亲和层析柱纯化融合蛋白,免疫BALB/c小鼠制备多克隆抗体,基于纯化获得的His-HcPR10重组蛋白和转HcPR10拟南芥总蛋白,分别利用ELISA和Western Blotting检测抗血清效价和特异性。结果表明:成功构建重组表达载体pET28a-HcPR10;正交结果显示诱导温度27℃,诱导转速200 r·min^-1,IPTG浓度0.7 mmol·L^-1,诱导时间6 h条件下可诱导表达大量可溶性目的蛋白;ELISA检测抗HcPR10血清效价达1∶243000,Western Blotting印迹结果显示制备的抗血清可以与重组蛋白和转基因拟南芥(Arabidopsis thaliana)中异源表达的HcPR10蛋白特异性结合。该研究获得了效价高、特异性强的盐穗木病程相关蛋白HcPR10抗血清,为进一步研究HcPR10的亚细胞定位及生物学功能奠定了基础。 展开更多
关键词 盐穗木病程相关蛋白hcpr10 原核表达 蛋白纯化 抗体制备及鉴定
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厂拌热再生设备选型对沥青混合料再生质量影响探析 被引量:3
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作者 李光林 张均辉 《辽宁省交通高等专科学校学报》 2009年第6期35-38,共4页
本文就沥青厂拌热再生设备选型进行阐述,探讨了不同种类厂拌热再生设备对沥青混合料的再生技术产生的作用等。并由此对高等级公路沥青路面养护维修工程中沥青混合料再生利用技术的完善具有一定参考价值。
关键词 厂拌热再生技术 再生设备 使用特点
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Hot central-plant recycling technology:A systematic review on raw materials and performance-influencing factors
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作者 Chengwei Xing Zhibin Chang +2 位作者 Wei Jiang Zhanchuang Han Mingchen Li 《Journal of Traffic and Transportation Engineering(English Edition)》 2025年第4期1040-1063,共24页
The hot central-plant recycling(HCPR)technology has been widely concerned by researchers in pavement engineering because of its excellent economic benefits and positive environmental outcomes.Over the last few years,t... The hot central-plant recycling(HCPR)technology has been widely concerned by researchers in pavement engineering because of its excellent economic benefits and positive environmental outcomes.Over the last few years,the application of HCPR technology in highway construction and maintenance has increasingly expanded.However,despite this wider adoption,critical issues concerning the composition of raw materials and performance-influencing factors of hot central-plant recycled asphalt mixtures(HCPRAM)necessitate careful consideration and deeper understanding.Therefore,conducting a detailed interpretation and systematic analysis of each component material and performance-influencing factors holds tremendous significance for advancing the design methodology,optimizing the production process,and enhancing the overall quality and durability of HCPRAM.This paper comprehensively reviews the current state-of-the-art research pertaining to the raw material composition and the crucial factors affecting the road performance of HCPRAM.Firstly,the functionality of recycled asphalt pavement(RAP)materials,virgin asphalt,virgin aggregates,rejuvenators,and fibers in the mixtures are introduced.Then,the influencing factors of the performance of HRAM are described in detail from both internal and external factors.Finally,the paper further discusses persistent challenges and knowledge gaps identified in the current research landscape of HCPR technology.Based on this critical analysis,pertinent recommendations are suggested to guide productive avenues for future research and development. 展开更多
关键词 hcpr technology hcprAM RAP Performance-influencing factors Production processes
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