AIM: To analyze the tissue morphologic phenotype and liver gene expression profile of hBIF transgenic mice. METHODS: Transgene expression was analyzed with RT-PCR and Western blotting. For one of the transgenic mouse ...AIM: To analyze the tissue morphologic phenotype and liver gene expression profile of hBIF transgenic mice. METHODS: Transgene expression was analyzed with RT-PCR and Western blotting. For one of the transgenic mouse lines, tissue expression pattern of the transgene was also examined with immunochemical methods. Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice. The liver gene expression profile of transgenic mice was analyzed with microchip, and some of bhe differentially expressed genes were verified with RT-PCR. RESULTS: The expressions of hBIF were shown in livers from 6 of 7 transgenic mouse lines. The overexpression of hB1F transgene did not cause pathological changes. Expressions of three genes were up-regulated, while down-regulation was observed for 25 genes. CONCLUSION: The overexpression of hBIF transgene may cause changes of gene expression profiles in the liver of transgenic mice.展开更多
Human B1 binding factor (hB1F) is a novel transcription factor cloned in our laboratory, which has the capacity of binding the Bl region of HBV enhancer Ⅱ (EN Ⅱ) and stimulating its activity. In this report, the fun...Human B1 binding factor (hB1F) is a novel transcription factor cloned in our laboratory, which has the capacity of binding the Bl region of HBV enhancer Ⅱ (EN Ⅱ) and stimulating its activity. In this report, the functional relationship between hBlF and other liver-enriched activators of EN Ⅱ was investigated. The results of CAT assay indicated that there was only additive effect between hB1F and HNF4 as well as HNF3β. On the contrary, the presence of both hBlF and HNF1 would result in significant functional synergism. Further GST pull down assay indicated that hBlF could interact with HNFl directly in vitro. This study provides the evidence that hB1F and HNF1 work syn-ergistically in the regulation of the activity of HBV EN Ⅱ.展开更多
We have cloned the cDNA of human nuclear receptor nr5a2(hb1f) gene and obtained its whole genomic sequence previously. In this work we carried out in-depth bioinformatic analysis on the genomic sequence of nr5a2(hb1f)...We have cloned the cDNA of human nuclear receptor nr5a2(hb1f) gene and obtained its whole genomic sequence previously. In this work we carried out in-depth bioinformatic analysis on the genomic sequence of nr5a2(hb1f) gene. Sequence comparison and prediction algorithms implicated that there might be additional coding regions in the 210 kb genomic sequence besides known exons, especially in the two largest introns. Comparison of the structures of nr5a loci in different species revealed distinguishable conservation and apparent gene duplication during evolution. The remarkable conservation among promoters of zebrafish, mouse and human nr5a2 genes suggested that they would be regulated by the same transcription factors.展开更多
Human nuclear receptor hB1F is a novel member of the fushi tarazu factor Ⅰ subfamily of nuclear receptor superfamily. The studies about its homologous genes indicate that hB1F may play a key role in regulating the me...Human nuclear receptor hB1F is a novel member of the fushi tarazu factor Ⅰ subfamily of nuclear receptor superfamily. The studies about its homologous genes indicate that hB1F may play a key role in regulating the metabolic homeostasis of cholesterol. After obtaining the founder mice carrying the trausgeue of hblf by microiujectiou, each founder was mated to normal C57 mouse and the positive F1 by PCR identification of the same founder were iutercrossed within sisters and brothers to establish the trausgeuic mouse lineage. The results of F1, F2 and offspring of test cross identification showed that the widely expressed hb1f trausgeuic mouse lineage was established successfully in this study. The tissue morphology of the trausgeuic lineage was also analyzed preliminarily.展开更多
基金Supported by the National Natural Science Foundation of China,No.39830360 the National "863" High Technology Research and Development Program of China,No.2001AA221261 the Qi Ming Xing Program from Shanghai Science and Technology Committee,No.01QA 140
文摘AIM: To analyze the tissue morphologic phenotype and liver gene expression profile of hBIF transgenic mice. METHODS: Transgene expression was analyzed with RT-PCR and Western blotting. For one of the transgenic mouse lines, tissue expression pattern of the transgene was also examined with immunochemical methods. Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice. The liver gene expression profile of transgenic mice was analyzed with microchip, and some of bhe differentially expressed genes were verified with RT-PCR. RESULTS: The expressions of hBIF were shown in livers from 6 of 7 transgenic mouse lines. The overexpression of hB1F transgene did not cause pathological changes. Expressions of three genes were up-regulated, while down-regulation was observed for 25 genes. CONCLUSION: The overexpression of hBIF transgene may cause changes of gene expression profiles in the liver of transgenic mice.
基金the National Natural Science Foundation of China (Grant No. 39893320) Science and Technology Fund of Shanghai (Grant No. 98JC14023).
文摘Human B1 binding factor (hB1F) is a novel transcription factor cloned in our laboratory, which has the capacity of binding the Bl region of HBV enhancer Ⅱ (EN Ⅱ) and stimulating its activity. In this report, the functional relationship between hBlF and other liver-enriched activators of EN Ⅱ was investigated. The results of CAT assay indicated that there was only additive effect between hB1F and HNF4 as well as HNF3β. On the contrary, the presence of both hBlF and HNF1 would result in significant functional synergism. Further GST pull down assay indicated that hBlF could interact with HNFl directly in vitro. This study provides the evidence that hB1F and HNF1 work syn-ergistically in the regulation of the activity of HBV EN Ⅱ.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39893320-4)the Chinese Human Genome Center at Shanghai (Grant No. CNCS-98-M-06)+1 种基金the Basic Research Program from the Ministry of Science Technology (Grant No. G19
文摘We have cloned the cDNA of human nuclear receptor nr5a2(hb1f) gene and obtained its whole genomic sequence previously. In this work we carried out in-depth bioinformatic analysis on the genomic sequence of nr5a2(hb1f) gene. Sequence comparison and prediction algorithms implicated that there might be additional coding regions in the 210 kb genomic sequence besides known exons, especially in the two largest introns. Comparison of the structures of nr5a loci in different species revealed distinguishable conservation and apparent gene duplication during evolution. The remarkable conservation among promoters of zebrafish, mouse and human nr5a2 genes suggested that they would be regulated by the same transcription factors.
文摘Human nuclear receptor hB1F is a novel member of the fushi tarazu factor Ⅰ subfamily of nuclear receptor superfamily. The studies about its homologous genes indicate that hB1F may play a key role in regulating the metabolic homeostasis of cholesterol. After obtaining the founder mice carrying the trausgeue of hblf by microiujectiou, each founder was mated to normal C57 mouse and the positive F1 by PCR identification of the same founder were iutercrossed within sisters and brothers to establish the trausgeuic mouse lineage. The results of F1, F2 and offspring of test cross identification showed that the widely expressed hb1f trausgeuic mouse lineage was established successfully in this study. The tissue morphology of the trausgeuic lineage was also analyzed preliminarily.
