目的建造BALB/c小鼠背部银屑病样模型,探讨中成药银屑平丸治疗银屑病的可能作用机制。方法选取48只BALB/c小鼠,随机分为空白组、模型对照组、雷公藤组、银屑平丸高、中、低剂量组,8只/组。给予除空白组外其余小鼠背部皮肤外涂咪喹莫特...目的建造BALB/c小鼠背部银屑病样模型,探讨中成药银屑平丸治疗银屑病的可能作用机制。方法选取48只BALB/c小鼠,随机分为空白组、模型对照组、雷公藤组、银屑平丸高、中、低剂量组,8只/组。给予除空白组外其余小鼠背部皮肤外涂咪喹莫特软膏连续8 d,1次/d,建立模型小鼠。末次给药后,观察小鼠背部皮肤进行皮损面积和严重程度指数(psoriasis area and serenty index,PASI)评分;检测血清中IL-17、IL-23水平;取背部皮肤组织进行组织病理比较。结果 (1)银屑平丸各组和雷公藤组对银屑病样小鼠背部皮肤PASI评分比较无明显的差异(P>0.05),而较模型对照组和空白组评分均有明显的差异(P<0.05)。(2)各组银屑病样小鼠外周血IL-17和IL-23含量明显高于空白组(P>0.05)。而银屑平丸各组和雷公藤组均与模型对照组外周血中含量有明显的差异(P<0.05)。(3)银屑平丸各组和雷公藤组对银屑病样小鼠背部皮肤组织病理变化较模型对照组和空白组有明显差异(P<0.05)。结论银屑平丸改善银屑病模型小鼠的作用机制可能通过抑制IL-17、IL-23,起到减轻小鼠银屑病样皮损的发生、发展的作用。展开更多
AIM:To develop the 17-item Asthenopia Survey Questionnaire(ASQ)-17 by Rasch analysis,and to generate a predictiveness score.METHODS:Totally 739 participants were recruited and 680 were involved in the result analysis ...AIM:To develop the 17-item Asthenopia Survey Questionnaire(ASQ)-17 by Rasch analysis,and to generate a predictiveness score.METHODS:Totally 739 participants were recruited and 680 were involved in the result analysis in this prospective,cross-sectional study.Three rounds of Rasch analysis were used to analyze the psychometric characteristics of items and options.RESULTS:Phase 1 assessed the original ASQ-19,adjusted the item scoring mode to a four-point Likert response rating scale and combined the 18th and 19th items into a new item.Phase 2 deleted the 11th item.Phases 3 and 4 assessed the new ASQ-17.All the evaluation indexes of ASQ-17 were acceptable.The Infit and Outfit MnSq values of items were 0.67-1.48,the variance explained by the principal component and the unexplained variance explained by the first contrast were 53.90%-59.40%and 1.50-1.80 in three dimensions.The curve peaks of scores in each dimension were separated and in the same order.The PSR and PSI values were 2.80 and 0.89,respectively.The mean scores of dimensions A(9.5±4.1 vs 3.5±3.2),B(7.3±3.3 vs 2.5±2.7),C(4.3±2.2 vs 1.4±2.0)and total(21.1±8.1 vs 7.4±7.0)in asthenopia participants were significantly higher than those without asthenopia(all P<0.001).The area under the curve in two groups was 0.899(P<0.001).Youden’s index was up to the maximum value of 0.784 when the cut-off value was 12.5.CONCLUSION:ASQ-17 has stronger option sorting and suitability than ASQ-19.It is an effective assessment tool for asthenopia with an optimal cut-off threshold value of 12.5,which is suitable for diagnosis and curative effect evaluation.展开更多
文摘目的建造BALB/c小鼠背部银屑病样模型,探讨中成药银屑平丸治疗银屑病的可能作用机制。方法选取48只BALB/c小鼠,随机分为空白组、模型对照组、雷公藤组、银屑平丸高、中、低剂量组,8只/组。给予除空白组外其余小鼠背部皮肤外涂咪喹莫特软膏连续8 d,1次/d,建立模型小鼠。末次给药后,观察小鼠背部皮肤进行皮损面积和严重程度指数(psoriasis area and serenty index,PASI)评分;检测血清中IL-17、IL-23水平;取背部皮肤组织进行组织病理比较。结果 (1)银屑平丸各组和雷公藤组对银屑病样小鼠背部皮肤PASI评分比较无明显的差异(P>0.05),而较模型对照组和空白组评分均有明显的差异(P<0.05)。(2)各组银屑病样小鼠外周血IL-17和IL-23含量明显高于空白组(P>0.05)。而银屑平丸各组和雷公藤组均与模型对照组外周血中含量有明显的差异(P<0.05)。(3)银屑平丸各组和雷公藤组对银屑病样小鼠背部皮肤组织病理变化较模型对照组和空白组有明显差异(P<0.05)。结论银屑平丸改善银屑病模型小鼠的作用机制可能通过抑制IL-17、IL-23,起到减轻小鼠银屑病样皮损的发生、发展的作用。
基金Supported by Wenzhou Science and Technology Bureau Project(No.Y2020036)the National Science Foundation of China(No.82000861)National Key Research and Development Program of China(No.2020YFC2008200).
文摘AIM:To develop the 17-item Asthenopia Survey Questionnaire(ASQ)-17 by Rasch analysis,and to generate a predictiveness score.METHODS:Totally 739 participants were recruited and 680 were involved in the result analysis in this prospective,cross-sectional study.Three rounds of Rasch analysis were used to analyze the psychometric characteristics of items and options.RESULTS:Phase 1 assessed the original ASQ-19,adjusted the item scoring mode to a four-point Likert response rating scale and combined the 18th and 19th items into a new item.Phase 2 deleted the 11th item.Phases 3 and 4 assessed the new ASQ-17.All the evaluation indexes of ASQ-17 were acceptable.The Infit and Outfit MnSq values of items were 0.67-1.48,the variance explained by the principal component and the unexplained variance explained by the first contrast were 53.90%-59.40%and 1.50-1.80 in three dimensions.The curve peaks of scores in each dimension were separated and in the same order.The PSR and PSI values were 2.80 and 0.89,respectively.The mean scores of dimensions A(9.5±4.1 vs 3.5±3.2),B(7.3±3.3 vs 2.5±2.7),C(4.3±2.2 vs 1.4±2.0)and total(21.1±8.1 vs 7.4±7.0)in asthenopia participants were significantly higher than those without asthenopia(all P<0.001).The area under the curve in two groups was 0.899(P<0.001).Youden’s index was up to the maximum value of 0.784 when the cut-off value was 12.5.CONCLUSION:ASQ-17 has stronger option sorting and suitability than ASQ-19.It is an effective assessment tool for asthenopia with an optimal cut-off threshold value of 12.5,which is suitable for diagnosis and curative effect evaluation.
