A few species in the genus Grateloupia （Halymeniaceae, Rhodophyta） have been investigated in detail with respect to morphological observations and molecular analyses. In this study, the au- thors document the vegeta...A few species in the genus Grateloupia （Halymeniaceae, Rhodophyta） have been investigated in detail with respect to morphological observations and molecular analyses. In this study, the au- thors document the vegetative and reproductive structures of two new species of Grateloupia, G. dalianensis H.W.Wang et D.Zhao, sp.nov, and G. yinggehaiensis H.W.Wang et R.X.Luan, sp.nov. They both have the morphological character that carpogonial ampullae and auxiliary cell ampullae are the simple Grateloupia-type. The two species can be distinguished from other species of the genus by their distinctive morphological features respectively. Bused on ribulose-l,5-bisphosphate carboxylase/oxygenase （rbcL） gene sequences, the phylogenetic tree obtained in the study indicated that they are both embedded within the Grateloupia clade. G. dalianensis clusters a subclade with G. asiatica, and G. yinggehaiensis forms a single monophyletic subclade with G. hawaiiana.展开更多
On the basis of morphological observations, life history and molecular phylogeny, Grateloupia yangjiangensis, which is similar to G. filicina, G. orientalis, G. catenata, and G. ramosissima in appearance, was re-exami...On the basis of morphological observations, life history and molecular phylogeny, Grateloupia yangjiangensis, which is similar to G. filicina, G. orientalis, G. catenata, and G. ramosissima in appearance, was re-examined. The results are as follows：（1） the auxiliary-cell ampullae of G. yangjiangensis were of Grateloupia type, thalli was fleshy and gelatinous in texture, and the erect axes were compressed; the cortex was 0.25–0.30 mm thick, consisting of five to seven outer layers, and there were five inner layers of triangular or stellate cells;（2） there was no filamentous stage in the development of the carpospores;（3） the ribulose-1,5-bisphosphate carboxylase/oxygenase gene（rbcL） sequence of four G. yangjiangensis examined showed that there was no intergeneric divergence among them, and for the phylogenetic tree, four sequences of G. yangjiangensis formed a single monophyletic subclade within the large Grateloupia clade of Halymeniaceae. In conclusion, G. yangjiangensis was a single species within the genus Grateloupia. This research provided criterion for identification and cultivation of G. yangjiangensis.展开更多
Grateloupia ramosa Wang & Luan sp. nov.(Halymeniaceae, Rhodophyta) is newly described from Hainan Province, southern China. The organism has the following morphological features:(1) purplish red, cartilaginous and...Grateloupia ramosa Wang & Luan sp. nov.(Halymeniaceae, Rhodophyta) is newly described from Hainan Province, southern China. The organism has the following morphological features:(1) purplish red, cartilaginous and lubricous thalli 5–10 cm in height;(2) compressed percurrent axes bearing abundant branches with opposite arrangement;(3) claw-like apices on top, constricted to 2–4 cm at the base;(4) cortex consisting of 3–6 layers of elliptical or anomalous cells and a medulla covered by compact medullary filaments;(5) reproductive structures distributed throughout the thallus, especially centralized at the bottom of the end portion of the branches; and(6) 4-celled Carpogonial branches and 3-celled auxiliary-cell branches, both of the Grateloupia-type. The morphological diff erences were supported by molecular phylogenetics based on ribulose-1, 5-bisphosphate carboxylase/oxygenase( rbc L) gene sequence analysis. There was only a 1 bp divergence between specimens collected from Wenchang and Lingshui of Hainan province. The new species was embedded in the large Grateloupia clade of the Halymeniaceae. The pairwise distances between G. ramosa and other species within Grateloupia ranged from 26 to 105 bp, within pairwise distances of 13–111 bp between species of the large genus Grateloupia in Halymeniaceae. Thus, we propose this new species as G. ramosa Wang & Luan sp. nov.展开更多
通过形态结构观察、生殖器官的发育和rbcL序列分析相结合的方法,发现了分布于海南省陵水新村的东方蜈蚣藻(Grateloupia orientalis S.-M.Lin et H.-Y.Liang)为海南新纪录种,该物种具有以下特征:藻体质地凝胶状或软骨质;皮层外部由5～6...通过形态结构观察、生殖器官的发育和rbcL序列分析相结合的方法,发现了分布于海南省陵水新村的东方蜈蚣藻(Grateloupia orientalis S.-M.Lin et H.-Y.Liang)为海南新纪录种,该物种具有以下特征:藻体质地凝胶状或软骨质;皮层外部由5～6层念珠状的细胞构成,内部由1～2层星形细胞构成;生殖枝丛由两个分枝组成;果孢子囊分布在除藻体基部外的皮层内。展开更多
Red alga species in the phylum Rhodophyta are ecologically and economically important,which are widely used as food and medicinal products because they are rich in bioactive compounds.Unfortunately,researchers often m...Red alga species in the phylum Rhodophyta are ecologically and economically important,which are widely used as food and medicinal products because they are rich in bioactive compounds.Unfortunately,researchers often meet challenges in identifying red algal species and understanding the evolutionary relationship among them.Shooting away these limitations necessitates detailed genomic studies,including those of cellular organelles.In this study,we sequenced circular organelle genomes of three red macroalgal species,Grateloupia asiatica,Pachymeniopsis lanceolata and Polyopes affinis,yielding two complete chloroplast genomes of P.lanceolata and P.affinis and one mitochondrial genome of P.affinis.The average chloroplast and mitochondrial genome sizes were 192,724 bp and 29,699 bp,respectively,which encoded 202 and 25 pro-teins,respectively,on average.The short-and long-repeat sequences,gene rearrangements,the nucleotide diversity,and phylogenetic relationship among these three species were analyzed.Memo:revise conjunctions and prepositions to form a clearer list(A,B,C,and D).The 194 chloroplast and 23 mitochondrial protein-coding genes shared by the species belonging to Florideophyceae and Bangiophyceae were used to reconstruct a maximum-likelihood phylogenetic tree.In addition,we developed 15 species-specific PCR markers,five for each species,using single-nucleotide polymorphism information.Our results should aid in identifying these species and deciphering the evolutionary relationship among species in the phylum Rhodophyta.展开更多
基金The general program of the National Natural Science Foundation of China (NSFC) under contract No.30870161
文摘A few species in the genus Grateloupia （Halymeniaceae, Rhodophyta） have been investigated in detail with respect to morphological observations and molecular analyses. In this study, the au- thors document the vegetative and reproductive structures of two new species of Grateloupia, G. dalianensis H.W.Wang et D.Zhao, sp.nov, and G. yinggehaiensis H.W.Wang et R.X.Luan, sp.nov. They both have the morphological character that carpogonial ampullae and auxiliary cell ampullae are the simple Grateloupia-type. The two species can be distinguished from other species of the genus by their distinctive morphological features respectively. Bused on ribulose-l,5-bisphosphate carboxylase/oxygenase （rbcL） gene sequences, the phylogenetic tree obtained in the study indicated that they are both embedded within the Grateloupia clade. G. dalianensis clusters a subclade with G. asiatica, and G. yinggehaiensis forms a single monophyletic subclade with G. hawaiiana.
基金The general program of the National Natural Science Foundation of China(NSFC)under contract Nos 30870161 and 31270251
文摘On the basis of morphological observations, life history and molecular phylogeny, Grateloupia yangjiangensis, which is similar to G. filicina, G. orientalis, G. catenata, and G. ramosissima in appearance, was re-examined. The results are as follows：（1） the auxiliary-cell ampullae of G. yangjiangensis were of Grateloupia type, thalli was fleshy and gelatinous in texture, and the erect axes were compressed; the cortex was 0.25–0.30 mm thick, consisting of five to seven outer layers, and there were five inner layers of triangular or stellate cells;（2） there was no filamentous stage in the development of the carpospores;（3） the ribulose-1,5-bisphosphate carboxylase/oxygenase gene（rbcL） sequence of four G. yangjiangensis examined showed that there was no intergeneric divergence among them, and for the phylogenetic tree, four sequences of G. yangjiangensis formed a single monophyletic subclade within the large Grateloupia clade of Halymeniaceae. In conclusion, G. yangjiangensis was a single species within the genus Grateloupia. This research provided criterion for identification and cultivation of G. yangjiangensis.
基金Supported by the National Natural Science Foundation of China(Nos.3087016131270251)
文摘Grateloupia ramosa Wang & Luan sp. nov.(Halymeniaceae, Rhodophyta) is newly described from Hainan Province, southern China. The organism has the following morphological features:(1) purplish red, cartilaginous and lubricous thalli 5–10 cm in height;(2) compressed percurrent axes bearing abundant branches with opposite arrangement;(3) claw-like apices on top, constricted to 2–4 cm at the base;(4) cortex consisting of 3–6 layers of elliptical or anomalous cells and a medulla covered by compact medullary filaments;(5) reproductive structures distributed throughout the thallus, especially centralized at the bottom of the end portion of the branches; and(6) 4-celled Carpogonial branches and 3-celled auxiliary-cell branches, both of the Grateloupia-type. The morphological diff erences were supported by molecular phylogenetics based on ribulose-1, 5-bisphosphate carboxylase/oxygenase( rbc L) gene sequence analysis. There was only a 1 bp divergence between specimens collected from Wenchang and Lingshui of Hainan province. The new species was embedded in the large Grateloupia clade of the Halymeniaceae. The pairwise distances between G. ramosa and other species within Grateloupia ranged from 26 to 105 bp, within pairwise distances of 13–111 bp between species of the large genus Grateloupia in Halymeniaceae. Thus, we propose this new species as G. ramosa Wang & Luan sp. nov.
文摘通过形态结构观察、生殖器官的发育和rbcL序列分析相结合的方法,发现了分布于海南省陵水新村的东方蜈蚣藻(Grateloupia orientalis S.-M.Lin et H.-Y.Liang)为海南新纪录种,该物种具有以下特征:藻体质地凝胶状或软骨质;皮层外部由5～6层念珠状的细胞构成,内部由1～2层星形细胞构成;生殖枝丛由两个分枝组成;果孢子囊分布在除藻体基部外的皮层内。
基金supported by the Korea Institute of Marine Science&Technology Promotion(KMIST)funded by the Ministry of Oceans and Fisheries,Korea[grant number 20210656].
文摘Red alga species in the phylum Rhodophyta are ecologically and economically important,which are widely used as food and medicinal products because they are rich in bioactive compounds.Unfortunately,researchers often meet challenges in identifying red algal species and understanding the evolutionary relationship among them.Shooting away these limitations necessitates detailed genomic studies,including those of cellular organelles.In this study,we sequenced circular organelle genomes of three red macroalgal species,Grateloupia asiatica,Pachymeniopsis lanceolata and Polyopes affinis,yielding two complete chloroplast genomes of P.lanceolata and P.affinis and one mitochondrial genome of P.affinis.The average chloroplast and mitochondrial genome sizes were 192,724 bp and 29,699 bp,respectively,which encoded 202 and 25 pro-teins,respectively,on average.The short-and long-repeat sequences,gene rearrangements,the nucleotide diversity,and phylogenetic relationship among these three species were analyzed.Memo:revise conjunctions and prepositions to form a clearer list(A,B,C,and D).The 194 chloroplast and 23 mitochondrial protein-coding genes shared by the species belonging to Florideophyceae and Bangiophyceae were used to reconstruct a maximum-likelihood phylogenetic tree.In addition,we developed 15 species-specific PCR markers,five for each species,using single-nucleotide polymorphism information.Our results should aid in identifying these species and deciphering the evolutionary relationship among species in the phylum Rhodophyta.
