A new algorithm is proposed to determine the actual length and the number of the protruding fibres of yarn based on a combination of image acquisition technology. First, a yarn hairiness image is obtained by the serie...A new algorithm is proposed to determine the actual length and the number of the protruding fibres of yarn based on a combination of image acquisition technology. First, a yarn hairiness image is obtained by the series of image processing procedures that include grayscale transformation,skew correction,yarn binary image acquisition and yarn core binary image obtaining. Then,the hairiness is realized in single pixel width by the usage of thinning algorithm. Finally, a baseline of yarn core margin is obtained,and pixels that match 8-neighbor template correctly are found by row scanning in a certain area. From this,these pixels are judged and the real crossover points of yarn core margin and hairiness,i. e.,the starting points of hairiness,are gained. The real length of the protruding fibres is calculated by tracking hairiness from the starting point constantly.展开更多
Yam hairiness is a complex concept, which generally cannot be completely defined by a single figure. Hairiness can be considered as the fiber ends and loops standing out from the main compact yarn body. Uster hairines...Yam hairiness is a complex concept, which generally cannot be completely defined by a single figure. Hairiness can be considered as the fiber ends and loops standing out from the main compact yarn body. Uster hairiness system characterizes the hairiness by H value, i.e. the mean value of the total length of all hairs within one centimeter of yarn. The raw data HI are in fact realization of spatial process （hairiness spatial process -- HSP） and can be used for more complex evaluation of hairiness characteristics in the space and frequency domain. The main aim of this contribution is description of some tools for spatial characterization of yarn hairiness. The simple methods for complex characterization of lISP statistical behavior （stationarity, independence, linearity etc. ） are presented. The techniques based on the embedding dimension and correlation integral or long-range dependences evaluation are discussed. The selected methods are core of HYARN program in MATLAB. Application of this program for deeper characterization of artificial data and cotton type yam are shown.展开更多
Traditional ring spinning system is associated with the several limitations, one of which is yarn hairiness. The awareness has been vibrantly growing among the spinning mills for reducing staple yarn hairiness because...Traditional ring spinning system is associated with the several limitations, one of which is yarn hairiness. The awareness has been vibrantly growing among the spinning mills for reducing staple yarn hairiness because of its detrimental effects on the subsequent processes (i.e., weaving, knitting, dyeing etc.) and the quality of resultant textile products. As a result, modified form of ring frame has attracted a lot of attention among the researchers for making yarn with the lower level of hairiness value. Besides the raw material parameters and some processing variables, spinning triangle (ST) is the most critical region that has decisive influence on the distribution of fiber tensions and their spatial orientations and shapes in staple yarn body. Thus, using appropriate methods to control the spinning triangle geometry actively has been a vital issue in the field of yarn hairiness reduction. In this paper, it is attempted to make an overview on the spinning triangle based modifications of ring frame that have been designed and destined to reduce hairiness. However, agent aided ring spinning system has also been briefly discussed in this paper as well.展开更多
In order to improve the quality of yarn,a research was carried out at ring spinning.A modifiedtraveller has been used to reduce the yarn hairiness vastly.According to the statistical analysis ofthe data,it was found t...In order to improve the quality of yarn,a research was carried out at ring spinning.A modifiedtraveller has been used to reduce the yarn hairiness vastly.According to the statistical analysis ofthe data,it was found that the geometrical shape of travellers is the key factor affecting the yarnhairiness at ring spinning.展开更多
Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herb...Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herbaceous plants,with notable applications in species such as Arabidopsis(Yin et al.,2024),soybean(Zhang et al.,2024),rice(Zhang et al.,2020),and Chinese cabbage(Li et al.,2021).However,its application in fruit trees is limited.This is primarily due to their long growth cycles and lack of rapid,efficient,and stable transgenic systems,which severely hinders foundational research involving plant genetic transformation(Mei et al.,2024).Furthermore,for subtropical fruit trees,the presence of recalcitrant seeds adds an extra layer of difficulty to genetic transformation(Umarani et al.,2015),as most methods rely on seed germination as a basis for transformation.展开更多
Drought is a major environmental constraint that significantly affects seedling emergence,yield,and quality of Tartary buckwheat,thereby hindering the development of its industry.However,the molecular mechanisms under...Drought is a major environmental constraint that significantly affects seedling emergence,yield,and quality of Tartary buckwheat,thereby hindering the development of its industry.However,the molecular mechanisms underlying drought tolerance genes in Tartary buckwheat remain largely unexplored.Alcohol dehydrogenase(ADH),an essential plant protein,plays a crucial role in growth,development,and stress responses;however,its specific role in drought resistance remains unclear.This study identifies an ADH gene,FtADH1,using a membership function value of drought tolerance(MFVD)combined with a genome-wide association study(GWAS)and transcriptomic profiles that confer drought tolerance in Tartary buckwheat.Our findings demonstrated that the overexpression of FtADH1 in Arabidopsis and Tartary buckwheat hairy roots enhances drought tolerance by promoting root elongation and mitigating elevated levels of reactive oxygen species(ROS).Our findings demonstrate that FtADH1 can enhance drought tolerance in Tartary buckwheat and Arabidopsis.This study identifies FtADH1 as a new regulator of Tartary buckwheat’s ROS levels and stress responses,functioning by regulating protective enzyme activities at a high level to scavenge ROS and modulating root growth under drought stress.Further,it identifies proteins interacting with FtADH1 through a prokaryotic expression pull-down assay combined with mass spectrometry,revealing that FtADH1 interacts explicitly with the S-adenosyl-L-methionine(SAM)synthetase protein,FtSAMS1.Overexpression of FtSAMS1 enhances ADH enzymatic activity,leading to increased SAM content in overexpressing Tartary buckwheat hairy roots under water-deficit conditions.Additionally,overexpression of FtSAMS1 induces a drought-resistant phenotype in Arabidopsis and Tartary buckwheat hairy roots under drought stress,revealing the biological function of FtADH1.Evolutionary analysis indicates that ADH1 in Fagopyrum species has undergone significant evolutionary events,including duplication and purifying selection,which may contribute to functional diversification and adaptive advantages such as drought resistance in cultivated buckwheat.In summary,this study suggests that FtADH1 is a key contributor to drought tolerance,and its interaction with FtSAMS1 offers promising potential for developing drought-resistant varieties in Tartary buckwheat and its relative species.展开更多
In this work,we apply tunneling formalism to analyze charged particles tunneling across a hairy black hole horizon.Such black hole solutions are essential for frameworks based on Horndeski's gravity theory.Applyin...In this work,we apply tunneling formalism to analyze charged particles tunneling across a hairy black hole horizon.Such black hole solutions are essential for frameworks based on Horndeski's gravity theory.Applying a semi-classical technique,we examine the tunneling of charged particles from a hairy black hole and derive the generic tunneling spectrum of released particles,ignoring self-gravitational and interaction.It is studied to ignore the back-reaction impact of the radiated particle on the hairy black hole.We analyze the properties of the black hole,such as temperature and entropy,under the influence of quantum gravity and also observe that the firstorder correction is present.We study tunneling radiation produced by a charged field equation in the presence of a generalized uncertainty effect.We modify the semi-classical technique by using the generalized uncertainty principle,the WKB approximation,and surface gravity.展开更多
The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop genetics.Within this system,single guide RNAs(sgRNAs)are instrumental in the precise cleavage of DNA double strands.However,the efficie...The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop genetics.Within this system,single guide RNAs(sgRNAs)are instrumental in the precise cleavage of DNA double strands.However,the efficiency of gene editing varies among sgRNAs,emphasizing the need to meticulously select target sites,especially in the context of Citrullus lanatus,a species notorious for its challenging and inefficient generation of transgenic plants through stable transformation.This study employed an Agrobacterium rhizogenes-mediated hairy root method to assess effective target sites for gene editing of ClCIPK17 across various Citrullus species.Hairy roots were successfully induced in different plant tissues at diverse growth stages of Citrullus lanatus,Citrullus mucosospermus,and Citrullus amarus.Employing a vector with two sgRNAs(sgRNA1 and sgRNA5)positioned within conserved regions of exon 1 and exon 5 of ClCIPK17 in the CRISPR/Cas9 system,targeted mutations were detected in 90.9%of accessions across the four Citrullus species.Notably,73.94%of all examined hairy roots exhibited mutations at the sgRNA1 site,while the sgRNA5 site showed no mutations.Among the 31 different mutation types identified at the sgRNA1 site,base deletion was the most prevalent.Using the sgRNA1 site of ClCIPK17,stable transgenic watermelon buds were obtained from explants,and the targeted mutations of the sgRNA1 site were confirmed.These findings underscore the viability of the hairy root transformation system in assessing the editing efficiency of sgRNA targets in diverse Citrullus species.展开更多
Alfalfa(Medicago sativa.L.)is a globally significant autotetraploid legume forage crop.However,despite its importance,establishing efficient gene editing systems for cultivated alfalfa remains a formidable challenge.I...Alfalfa(Medicago sativa.L.)is a globally significant autotetraploid legume forage crop.However,despite its importance,establishing efficient gene editing systems for cultivated alfalfa remains a formidable challenge.In this study,we pioneered the development of a highly effective ultrasonic-assisted leaf disc transformation system for Gongnong 1 alfalfa,a variety widely cultivated in Northeast China.Subsequently,we created a single transcript CRISPR/Cas9(CRISPR_2.0)toolkit,incorporating multiplex gRNAs,designed for gene editing in Gongnong 1.Both Cas9 and gRNA scaffolds were under the control of the Arabidopsis ubiquitin-10 promoter,a widely employed polymeraseⅡconstitutive promoter known for strong transgene expression in dicots.To assess the toolkit’s efficiency,we targeted PALM1,a gene associated with a recognizable multifoliate phenotype.Utilizing the CRISPR_2.0 toolkit,we directed PALM1 editing at two sites in the wild-type Gongnong 1.Results indicated a 35.1%occurrence of editing events all in target 2 alleles,while no mutations were detected at target 1 in the transgenic-positive lines.To explore more efficient sgRNAs,we developed a rapid,reliable screening system based on Agrobacterium rhizogenes-mediated hairy root transformation,incorporating the visible reporter MtLAP1.This screening system demonstrated that most purple visible hairy roots underwent gene editing.Notably,sgRNA3,with an 83.0%editing efficiency,was selected using the visible hairy root system.As anticipated,tetra-allelic homozygous palm1 mutations exhibited a clear multifoliate phenotype.These palm1 lines demonstrated an average crude protein yield increase of 21.5%compared to trifoliolate alfalfa.Our findings highlight the modified CRISPR_2.0 system as a highly efficient and robust gene editing tool for autotetraploid alfalfa.展开更多
Many economically important crops and vegetables belonging to the cruciferous family are heavily endangered by clubroot disease caused by Plasmodiophora brassicae infection.Breeding of clubroot resistant cultivars bas...Many economically important crops and vegetables belonging to the cruciferous family are heavily endangered by clubroot disease caused by Plasmodiophora brassicae infection.Breeding of clubroot resistant cultivars based on mapping and cloning of resistant genes is commonly regarded as the most cost-effective and efficient way to fight against this disease.The traditional way of R gene functional validation requires stable transformation that is both time-and labor-consuming.In this study,a rapid and efficient hairy-root transgenic protocol mediated by Agrobacterium rhizogenes was developed.The transformation positive rate was over 80%in Brassica napus showed by GUS reporter gene and this transformation only took 1/6 of the time compared with stable transformation.The system was applicable to different B.napus varieties and other cruciferous crops including Brassica rapa and Brassica oleracea.In particular,two known CR genes,CRA3.7.1 and CRA8.2.4 were used respectively,as example to show that the system works well for CR gene study combined with subsequent P.brassicae infection in B.napus.Most importantly,it works both in over-expression that led to disease resistance,as well as in RNAi which led to disease susceptible phenotype.Therefore,this system can be used in batch-wise identification of CR genes,and also offered the possibility of manipulating key genes within the P.brassicae genome that could improve our knowledge on host-pathogen interaction.展开更多
We investigate the observational appearance of static and spherically symmetric hairy black holes in the framework of gravitational decoupling with the weak energy condition(WEC). Two types of thin illumination condit...We investigate the observational appearance of static and spherically symmetric hairy black holes in the framework of gravitational decoupling with the weak energy condition(WEC). Two types of thin illumination conditions are studied: spherical accretion and disk accretion. As the hairy parameter increases, the size of the photon sphere and photon rings in both models decreases, and the overall luminosity attenuation becomes more pronounced. In spherical accretion, the luminosity of infalling accretion is significantly lower than that of stationary accretion. In disk accretion the luminosity of the black hole is contributed by direct emission, the lensing ring and the photon ring. Employing four types of astrophysical disk luminosity model,we investigate the appearance of halos and note that their luminosities do not superimpose when the source is on or beyond the innermost stable circular orbit.展开更多
Jute(Corchorus capsularis L.)is the second most important natural plant fiber source after cotton.However,developing an efficient gene editing system for jute remains a challenge.In this study,the transgenic hairy roo...Jute(Corchorus capsularis L.)is the second most important natural plant fiber source after cotton.However,developing an efficient gene editing system for jute remains a challenge.In this study,the transgenic hairy root system mediated by Agrobacterium rhizogenes strain K599 was developed for Meifeng 4,an elite jute variety widely cultivated in China.The transgenic hairy root system for jute was verified by subcellular localization and bimolecular fluorescence complementation(BiFC)assays.The CHLOROPLASTOS ALTERADOS 1(CcCLA1)gene,which is involved in the development of chloroplasts,was targeted for editing at two sites in Meifeng 4.Based on this hairy root transformation,the gRNA scaffold was placed under the control of cotton ubiquitin GhU6.7 and-GhU6.9 promoters,respectively,to assess the efficiency of gene editing.Results indicated the 50.0%(GhU6.7)and 38.5%(GhU6.9)editing events in the target 2 alleles(gRNA2),but no mutation was detected in the target 1 allele(gRNA1)in transgenic-positive hairy roots.CcCLA1 gene editing at gRNA2 under the control of GhU6.7 in Meifeng 4 was also carried out by Agrobacterium tumefaciens-mediated transformation.Two CcCLA1 mutants were albinic,with a gene editing efficiency of 5.3%.These findings confirm that the CRISPR/Cas9 system,incorporating promoter GhU6.7,can be used as a gene editing tool for jute.展开更多
Hairy cell leukemia(HCL)is an uncommon mature B-cell malignancy characterized by a typical morphology,immunophenotype,and clinical profile.The vast majority of HCL patients harbor the canonical BRAF V600E mutation whi...Hairy cell leukemia(HCL)is an uncommon mature B-cell malignancy characterized by a typical morphology,immunophenotype,and clinical profile.The vast majority of HCL patients harbor the canonical BRAF V600E mutation which has become a rationalized target of the subsequently deregulated RAS-RAF-MEK-MAPK signaling pathway in HCL patients who have relapsed or who are refractory to front-line therapy.However,several HCL patients with a classical phenotype display non-canonical BRAF mutations or rearrangements.These include sequence variants within alternative exons and an oncogenic fusion with the IGH gene.Care must be taken in the molecular diagnostic work-up of patients with typical HCL but without the BRAF V600E to include investigation of these uncommon mechanisms.Identification,functional characterization,and reporting of further such patients is likely to provide insights into the pathogenesis of HCL and enable rational selection of targeted inhibitors in such patients if required.展开更多
Panax ginseng C.A.Mey.is an important plant species used in traditional Chinese medicine,whose primary active ingredient is a ginsenoside.Ginsenoside biosynthesis is not only regulated by transcription factors but als...Panax ginseng C.A.Mey.is an important plant species used in traditional Chinese medicine,whose primary active ingredient is a ginsenoside.Ginsenoside biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural genes.Nonetheless,the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary metabolites.Squalene epoxidase(SQE)is a key enzyme in the mevalonic acid pathway,which affects the biosynthesis of secondary metabolites such as terpenoid.Using ginseng transcriptome,expression,and ginsenoside content databases,this study employed bioinformatic methods to systematically analyze the genes encoding SQE in ginseng.We first selected six PgSQE candidates that were closely involved in ginsenoside biosynthesis and then identified PgSQE08-01 to be highly associated with ginsenoside biosynthesis.Next,we constructed the overexpression vector pCAMBIA3301-PgSQE08-01 and the RNAi vector pART27-PgSQE08-01 and transformed ginseng adventitious roots using Agrobacterium rhizogenes,to obtain positive hairy-root clones.Thereafter,quantitative reverse transcriptionpolymerase chain reaction and high-performance liquid chromatography were used to determine the expression of relevant genes and ginsenoside content,respectively.Then,we focused on the function of PgSQE08-01 gene,which was noted to be involved in ginsenoside biosynthesis.Thus,these findings not only provided a molecular basis for the identification of important functional genes in ginseng but also enriched genetic resources for the biosynthesis of ginsenosides using synthetic biology.展开更多
Identification of management practices that can improve soil health is critical to improving the sustainability of soybean [Glycine max (L.) Merr.] production. The objective of this study was to examine the long-term ...Identification of management practices that can improve soil health is critical to improving the sustainability of soybean [Glycine max (L.) Merr.] production. The objective of this study was to examine the long-term effects of continuous soybean, corn-soybean, and soybean-cotton rotations with chicken litter and cover crops (hairy vetch, wheat, fallow) on soil health parameters, including nutrient accumulation and soil organic matter dynamics under a split plot design. The depth intervals of soil sampling were 0 - 15, 15 - 30, 30 - 60, and 60 - 90 cm. Chicken litter resulted in 62.1% and 32.8% higher water extractable organic soil N content than fallow and wheat, respectively, in the surface 0 - 15 cm of soil only. However, there was no significant difference in 1-day Solvita respiration, water extractable organic C, C/N ratio, health score, moisture, earthworm, organic matter, pH, or CEC of soil among fallow, hairy vetch, chicken litter, and wheat regardless of soil depth. Unexpectedly, annual application of chicken litter at 4.4 Mg ha−1 as an N source or growing a winter-season cover crop such as hairy vetch or wheat for continuous 16 years did not significantly increase soil organic matter or water extractable organic soil C. Annual application of chicken litter at 4.4 metric tons (Mg) ha–1 for 16 years increased soil nitrate-nitrogen (NO3−-N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), and zinc (Zn) contents by 92%, 400%, 134%, 20%, 43%, 206%, and 430% in 0 - 15 cm depth compared with their initial soil values, respectively, extracted with Haney H3A-2 (2 g L–1 lithium citrate + 0.6 g L–1 citric acid + 0.4 g L–1 malic acid + 0.4 g L–1 oxalic acid) solution. The increases enhanced soil supply of these nutrients to following crops, but also increased the risks of losing them to the environment. Hairy vetch caused higher H3A extracted soil manganese (Mn) content than fallow and chicken litter in 0 - 60 cm. There was no significant difference in 1-day Solvita respiration, water extractable organic C and N, health score, moisture, organic matter, pH, CEC, or population of earthworm of soil among continuous soybean, corn-soybean, and soybean-cotton in any soil depth. Another major finding of this study was that continuous soybean exerted no adverse effect on soil health relative to the commonly used corn (Zea mays L.)-soybean and soybean-cotton (Gossypium hirsutum L.) rotations under no-tillage after 16 years. To mitigate the risks of nutrient runoff and leaching from long-term chicken litter application, we recommend reducing litter application rates and integrating cover crops into crop rotations to enhance nutrient cycling and reduce environmental impacts.展开更多
In this paper,we investigate the gravitational quasi-normal modes(QNMs)of Horndeski hairy black holes.By using the ordinary finite element method,the relations between the QNMs and the parameters of black holes are mi...In this paper,we investigate the gravitational quasi-normal modes(QNMs)of Horndeski hairy black holes.By using the ordinary finite element method,the relations between the QNMs and the parameters of black holes are minutely shown.Both the oscillating frequency and the decay rate increase with the increase of the scalar charge Q.While the oscillating frequency and the decay rate decrease with the black hole mass M increasing.And the quasi-period of the oscillation decreases when the angular momentum l of the perturbation field increases.However,the decay rate has barely changed for different values of 1.Thus,the numerical results indicate that Horndeski black holes are stable.展开更多
The artemisinin accumulation in the hairy root cultures of Artemisia annua L. was enhanced via a treatment of three fungal elicitors separately ( Verticillium dahliae Kleb., Rhizopus stolonifer (Ehrenb. ex ...The artemisinin accumulation in the hairy root cultures of Artemisia annua L. was enhanced via a treatment of three fungal elicitors separately ( Verticillium dahliae Kleb., Rhizopus stolonifer (Ehrenb. ex Fr.) Vuill and Colletotrichum dematium (Pers.) Grove). Among these three elicitors, V. dahliae had the highest inducing efficiency, but none of them manifests any noticeable effects on the cell growth of the hairy root cultures. The artemisinin content of the hairy root cultures treated with V. dahliae elicitor was 1.12 mg/g DW, which was 45% higher than the control (0.77 mg/g DW). The results showed that elicitation was dependent on the elicitor concentration, the incubation period and the physiological stage at which the hairy root cultures were treated. In addition, the authors found that for V. dahliae , the optimum concentration was 0.4 mg carbohydrate per millilitre medium, the strongest response of A. annua hairy root cultures to the elicitation was at the late exponential growth stage, and the highest artemisinin content of the hairy root cultures was on the 4th day post treatment.展开更多
The technological process of compact spinning and the compact procedure of fiber band in compact field are briefly illustrated. The motions of fiber band in compact field are discussed theoretically from which tilting...The technological process of compact spinning and the compact procedure of fiber band in compact field are briefly illustrated. The motions of fiber band in compact field are discussed theoretically from which tilting angle of suction slot in profile tube, additional twists created by fiber band’s rotating around its own axis and ultimate twists in compact yarn are deduced accordingly. The existence of additional twists is also verified through experiments.展开更多
Variations in leaf morphological characteristics have been extensively studied at both interand intraspecific levels although not explicitly on paper birch (Betula papyrifera Marsh). Paper birch populations might have...Variations in leaf morphological characteristics have been extensively studied at both interand intraspecific levels although not explicitly on paper birch (Betula papyrifera Marsh). Paper birch populations might have considerable genotypic and leaf morphological variations that have allowed them to inhabit wide environmental gradients. In this study, we analyzed variations in leaf morphological characteristics in 23 paper birch populations collected across Canada and grown in a greenhouse. Furthermore, we examined whether the variations in leaf morphological characteristics observed were related to the climate of the population’s origin. We found significant genotypic differences in all leaf morphological characteristics (p < 0.05) measured among the birch populations. Thus, we expected that the morphological variations in birch might be related to natural diversity in birch populations due to environmental differences at habitat origin. Principal component analysis (PCA) reduced thirteen leaf morphological variables to five principal components (PC) explaining 84.74% of the total variance in the original data. PCs accumulated with specific leaf area, petiole and leaf width were positively related to latitudinal, longitudinal, and elevational gradients at the population’s origin. Unpredictably, these PCs were significantly negatively correlated to precipitation and aridity index at the origin. Thus, we analyzed if correlations within leaf morphological characteristics had supported the birch populations to acclimate and produce unpredictable relations with the environment of origin. Our results showed that the populations originated in limited precipitation (during growing season) had large leaf width and petiole size but low leaf hairs on adaxial surface. Thus, all these leaf morphological features provide a basis for the birch to reduce water loss from leaves and balance water use efficiency in reduced precipitation. Furthermore, the leaf characteristics measured may also include phenotypic plasticity of the birch as an acclimation to the environment as in the greenhouse.展开更多
基金National Natural Science Foundation of China(No.61301276)Xi’an Polytechnic University Young Scholar Backbone Supporting Plan,ChinaDiscipline Construction Funds of Xi’an Polytechnic University,China(No.107090811)
文摘A new algorithm is proposed to determine the actual length and the number of the protruding fibres of yarn based on a combination of image acquisition technology. First, a yarn hairiness image is obtained by the series of image processing procedures that include grayscale transformation,skew correction,yarn binary image acquisition and yarn core binary image obtaining. Then,the hairiness is realized in single pixel width by the usage of thinning algorithm. Finally, a baseline of yarn core margin is obtained,and pixels that match 8-neighbor template correctly are found by row scanning in a certain area. From this,these pixels are judged and the real crossover points of yarn core margin and hairiness,i. e.,the starting points of hairiness,are gained. The real length of the protruding fibres is calculated by tracking hairiness from the starting point constantly.
基金Supported by the research project"Textile center"of Czech Ministry of Education1M4674788501
文摘Yam hairiness is a complex concept, which generally cannot be completely defined by a single figure. Hairiness can be considered as the fiber ends and loops standing out from the main compact yarn body. Uster hairiness system characterizes the hairiness by H value, i.e. the mean value of the total length of all hairs within one centimeter of yarn. The raw data HI are in fact realization of spatial process （hairiness spatial process -- HSP） and can be used for more complex evaluation of hairiness characteristics in the space and frequency domain. The main aim of this contribution is description of some tools for spatial characterization of yarn hairiness. The simple methods for complex characterization of lISP statistical behavior （stationarity, independence, linearity etc. ） are presented. The techniques based on the embedding dimension and correlation integral or long-range dependences evaluation are discussed. The selected methods are core of HYARN program in MATLAB. Application of this program for deeper characterization of artificial data and cotton type yam are shown.
文摘Traditional ring spinning system is associated with the several limitations, one of which is yarn hairiness. The awareness has been vibrantly growing among the spinning mills for reducing staple yarn hairiness because of its detrimental effects on the subsequent processes (i.e., weaving, knitting, dyeing etc.) and the quality of resultant textile products. As a result, modified form of ring frame has attracted a lot of attention among the researchers for making yarn with the lower level of hairiness value. Besides the raw material parameters and some processing variables, spinning triangle (ST) is the most critical region that has decisive influence on the distribution of fiber tensions and their spatial orientations and shapes in staple yarn body. Thus, using appropriate methods to control the spinning triangle geometry actively has been a vital issue in the field of yarn hairiness reduction. In this paper, it is attempted to make an overview on the spinning triangle based modifications of ring frame that have been designed and destined to reduce hairiness. However, agent aided ring spinning system has also been briefly discussed in this paper as well.
文摘In order to improve the quality of yarn,a research was carried out at ring spinning.A modifiedtraveller has been used to reduce the yarn hairiness vastly.According to the statistical analysis ofthe data,it was found that the geometrical shape of travellers is the key factor affecting the yarnhairiness at ring spinning.
基金funded by the Key-Area Research and Development Program of Guangdong Province(Grant No.2022B0202070002)the Guangxi Science and Technology Major Program(Grant No.GuikeAA23023007-2)+1 种基金the Guangdong Province Modern Agricultural Industry Technology System Innovation Team Construction Project(2024CXTD19)Guangdong Basic and Applied Basic Research Foundation(Grant No.2023A1515010303)。
文摘Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herbaceous plants,with notable applications in species such as Arabidopsis(Yin et al.,2024),soybean(Zhang et al.,2024),rice(Zhang et al.,2020),and Chinese cabbage(Li et al.,2021).However,its application in fruit trees is limited.This is primarily due to their long growth cycles and lack of rapid,efficient,and stable transgenic systems,which severely hinders foundational research involving plant genetic transformation(Mei et al.,2024).Furthermore,for subtropical fruit trees,the presence of recalcitrant seeds adds an extra layer of difficulty to genetic transformation(Umarani et al.,2015),as most methods rely on seed germination as a basis for transformation.
基金supported by the National Natural Science Foundation of China(32372045)the Key Laboratory of Molecular Breeding for Grain and Oil Crops in Guizhou Province,China(Qiankehezhongyindi(2023)008)the Key Laboratory of Functional Agriculture of Guizhou Provincial Higher Education Institutions,China(Qianjiaoji(2023)007).
文摘Drought is a major environmental constraint that significantly affects seedling emergence,yield,and quality of Tartary buckwheat,thereby hindering the development of its industry.However,the molecular mechanisms underlying drought tolerance genes in Tartary buckwheat remain largely unexplored.Alcohol dehydrogenase(ADH),an essential plant protein,plays a crucial role in growth,development,and stress responses;however,its specific role in drought resistance remains unclear.This study identifies an ADH gene,FtADH1,using a membership function value of drought tolerance(MFVD)combined with a genome-wide association study(GWAS)and transcriptomic profiles that confer drought tolerance in Tartary buckwheat.Our findings demonstrated that the overexpression of FtADH1 in Arabidopsis and Tartary buckwheat hairy roots enhances drought tolerance by promoting root elongation and mitigating elevated levels of reactive oxygen species(ROS).Our findings demonstrate that FtADH1 can enhance drought tolerance in Tartary buckwheat and Arabidopsis.This study identifies FtADH1 as a new regulator of Tartary buckwheat’s ROS levels and stress responses,functioning by regulating protective enzyme activities at a high level to scavenge ROS and modulating root growth under drought stress.Further,it identifies proteins interacting with FtADH1 through a prokaryotic expression pull-down assay combined with mass spectrometry,revealing that FtADH1 interacts explicitly with the S-adenosyl-L-methionine(SAM)synthetase protein,FtSAMS1.Overexpression of FtSAMS1 enhances ADH enzymatic activity,leading to increased SAM content in overexpressing Tartary buckwheat hairy roots under water-deficit conditions.Additionally,overexpression of FtSAMS1 induces a drought-resistant phenotype in Arabidopsis and Tartary buckwheat hairy roots under drought stress,revealing the biological function of FtADH1.Evolutionary analysis indicates that ADH1 in Fagopyrum species has undergone significant evolutionary events,including duplication and purifying selection,which may contribute to functional diversification and adaptive advantages such as drought resistance in cultivated buckwheat.In summary,this study suggests that FtADH1 is a key contributor to drought tolerance,and its interaction with FtSAMS1 offers promising potential for developing drought-resistant varieties in Tartary buckwheat and its relative species.
基金funded by the National Natural Science Foundation of China under Grant No.11975145。
文摘In this work,we apply tunneling formalism to analyze charged particles tunneling across a hairy black hole horizon.Such black hole solutions are essential for frameworks based on Horndeski's gravity theory.Applying a semi-classical technique,we examine the tunneling of charged particles from a hairy black hole and derive the generic tunneling spectrum of released particles,ignoring self-gravitational and interaction.It is studied to ignore the back-reaction impact of the radiated particle on the hairy black hole.We analyze the properties of the black hole,such as temperature and entropy,under the influence of quantum gravity and also observe that the firstorder correction is present.We study tunneling radiation produced by a charged field equation in the presence of a generalized uncertainty effect.We modify the semi-classical technique by using the generalized uncertainty principle,the WKB approximation,and surface gravity.
基金supported by the Basic Research Funds of Chinese Academy of Agricultural Sciences(Grant No.1610192023201)the National Key Research and Development Program of China(Grant No.SQ2023YFE0201239)+3 种基金China Agriculture Research System of MOF and MARA(Grant No.CARS-25-03)the Major Science and Technology Projects of Henan Province(Grant No.221100110400)the Funding of Joint Research on Agricultural Variety Improvement of Henan Province(Grant No.2022010503)the Agricultural Science and Technology Innovation Program(Grant No.CAAS-ASTIP-2021-ZFRI)。
文摘The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop genetics.Within this system,single guide RNAs(sgRNAs)are instrumental in the precise cleavage of DNA double strands.However,the efficiency of gene editing varies among sgRNAs,emphasizing the need to meticulously select target sites,especially in the context of Citrullus lanatus,a species notorious for its challenging and inefficient generation of transgenic plants through stable transformation.This study employed an Agrobacterium rhizogenes-mediated hairy root method to assess effective target sites for gene editing of ClCIPK17 across various Citrullus species.Hairy roots were successfully induced in different plant tissues at diverse growth stages of Citrullus lanatus,Citrullus mucosospermus,and Citrullus amarus.Employing a vector with two sgRNAs(sgRNA1 and sgRNA5)positioned within conserved regions of exon 1 and exon 5 of ClCIPK17 in the CRISPR/Cas9 system,targeted mutations were detected in 90.9%of accessions across the four Citrullus species.Notably,73.94%of all examined hairy roots exhibited mutations at the sgRNA1 site,while the sgRNA5 site showed no mutations.Among the 31 different mutation types identified at the sgRNA1 site,base deletion was the most prevalent.Using the sgRNA1 site of ClCIPK17,stable transgenic watermelon buds were obtained from explants,and the targeted mutations of the sgRNA1 site were confirmed.These findings underscore the viability of the hairy root transformation system in assessing the editing efficiency of sgRNA targets in diverse Citrullus species.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA26030301)Hohhot Key R&D Project(2023-JBGSS-1),the National Natural Science Foundation of China(U23A200206,32071864,32325035)+1 种基金the Taishan Scholar Program of Shandong(to Chunxiang Fu)the Shandong Provincial Natural Science Foundation(ZR202210270038)。
文摘Alfalfa(Medicago sativa.L.)is a globally significant autotetraploid legume forage crop.However,despite its importance,establishing efficient gene editing systems for cultivated alfalfa remains a formidable challenge.In this study,we pioneered the development of a highly effective ultrasonic-assisted leaf disc transformation system for Gongnong 1 alfalfa,a variety widely cultivated in Northeast China.Subsequently,we created a single transcript CRISPR/Cas9(CRISPR_2.0)toolkit,incorporating multiplex gRNAs,designed for gene editing in Gongnong 1.Both Cas9 and gRNA scaffolds were under the control of the Arabidopsis ubiquitin-10 promoter,a widely employed polymeraseⅡconstitutive promoter known for strong transgene expression in dicots.To assess the toolkit’s efficiency,we targeted PALM1,a gene associated with a recognizable multifoliate phenotype.Utilizing the CRISPR_2.0 toolkit,we directed PALM1 editing at two sites in the wild-type Gongnong 1.Results indicated a 35.1%occurrence of editing events all in target 2 alleles,while no mutations were detected at target 1 in the transgenic-positive lines.To explore more efficient sgRNAs,we developed a rapid,reliable screening system based on Agrobacterium rhizogenes-mediated hairy root transformation,incorporating the visible reporter MtLAP1.This screening system demonstrated that most purple visible hairy roots underwent gene editing.Notably,sgRNA3,with an 83.0%editing efficiency,was selected using the visible hairy root system.As anticipated,tetra-allelic homozygous palm1 mutations exhibited a clear multifoliate phenotype.These palm1 lines demonstrated an average crude protein yield increase of 21.5%compared to trifoliolate alfalfa.Our findings highlight the modified CRISPR_2.0 system as a highly efficient and robust gene editing tool for autotetraploid alfalfa.
基金supported by grants from the Wuhan Science and Technology Major Project on Key techniques of biological breeding and Breeding of new varieties(Grant No.2022021302024851)the special project for sustainable development agenda of innovation demonstration zone(Grant No.202204AC100001-A04)the National Key R&D Program of China(Grant No.2022YFD1200400)。
文摘Many economically important crops and vegetables belonging to the cruciferous family are heavily endangered by clubroot disease caused by Plasmodiophora brassicae infection.Breeding of clubroot resistant cultivars based on mapping and cloning of resistant genes is commonly regarded as the most cost-effective and efficient way to fight against this disease.The traditional way of R gene functional validation requires stable transformation that is both time-and labor-consuming.In this study,a rapid and efficient hairy-root transgenic protocol mediated by Agrobacterium rhizogenes was developed.The transformation positive rate was over 80%in Brassica napus showed by GUS reporter gene and this transformation only took 1/6 of the time compared with stable transformation.The system was applicable to different B.napus varieties and other cruciferous crops including Brassica rapa and Brassica oleracea.In particular,two known CR genes,CRA3.7.1 and CRA8.2.4 were used respectively,as example to show that the system works well for CR gene study combined with subsequent P.brassicae infection in B.napus.Most importantly,it works both in over-expression that led to disease resistance,as well as in RNAi which led to disease susceptible phenotype.Therefore,this system can be used in batch-wise identification of CR genes,and also offered the possibility of manipulating key genes within the P.brassicae genome that could improve our knowledge on host-pathogen interaction.
基金supported by the National Natural Science Foundation of China (NSFC) with grant nos 12175212, 12275183 and 12275184。
文摘We investigate the observational appearance of static and spherically symmetric hairy black holes in the framework of gravitational decoupling with the weak energy condition(WEC). Two types of thin illumination conditions are studied: spherical accretion and disk accretion. As the hairy parameter increases, the size of the photon sphere and photon rings in both models decreases, and the overall luminosity attenuation becomes more pronounced. In spherical accretion, the luminosity of infalling accretion is significantly lower than that of stationary accretion. In disk accretion the luminosity of the black hole is contributed by direct emission, the lensing ring and the photon ring. Employing four types of astrophysical disk luminosity model,we investigate the appearance of halos and note that their luminosities do not superimpose when the source is on or beyond the innermost stable circular orbit.
基金supported by the National Natural Science Foundation of China (31771369)the Natural Science Foundation of Fujian, China (2023J01443)the China Agriculture Research System of the Ministry of Agriculture and MARA (CARS-16)
文摘Jute(Corchorus capsularis L.)is the second most important natural plant fiber source after cotton.However,developing an efficient gene editing system for jute remains a challenge.In this study,the transgenic hairy root system mediated by Agrobacterium rhizogenes strain K599 was developed for Meifeng 4,an elite jute variety widely cultivated in China.The transgenic hairy root system for jute was verified by subcellular localization and bimolecular fluorescence complementation(BiFC)assays.The CHLOROPLASTOS ALTERADOS 1(CcCLA1)gene,which is involved in the development of chloroplasts,was targeted for editing at two sites in Meifeng 4.Based on this hairy root transformation,the gRNA scaffold was placed under the control of cotton ubiquitin GhU6.7 and-GhU6.9 promoters,respectively,to assess the efficiency of gene editing.Results indicated the 50.0%(GhU6.7)and 38.5%(GhU6.9)editing events in the target 2 alleles(gRNA2),but no mutation was detected in the target 1 allele(gRNA1)in transgenic-positive hairy roots.CcCLA1 gene editing at gRNA2 under the control of GhU6.7 in Meifeng 4 was also carried out by Agrobacterium tumefaciens-mediated transformation.Two CcCLA1 mutants were albinic,with a gene editing efficiency of 5.3%.These findings confirm that the CRISPR/Cas9 system,incorporating promoter GhU6.7,can be used as a gene editing tool for jute.
文摘Hairy cell leukemia(HCL)is an uncommon mature B-cell malignancy characterized by a typical morphology,immunophenotype,and clinical profile.The vast majority of HCL patients harbor the canonical BRAF V600E mutation which has become a rationalized target of the subsequently deregulated RAS-RAF-MEK-MAPK signaling pathway in HCL patients who have relapsed or who are refractory to front-line therapy.However,several HCL patients with a classical phenotype display non-canonical BRAF mutations or rearrangements.These include sequence variants within alternative exons and an oncogenic fusion with the IGH gene.Care must be taken in the molecular diagnostic work-up of patients with typical HCL but without the BRAF V600E to include investigation of these uncommon mechanisms.Identification,functional characterization,and reporting of further such patients is likely to provide insights into the pathogenesis of HCL and enable rational selection of targeted inhibitors in such patients if required.
基金This work was supported by an award from the Department of Science and Technology of Jilin Province(20210402043GH and 20210204063YY).
文摘Panax ginseng C.A.Mey.is an important plant species used in traditional Chinese medicine,whose primary active ingredient is a ginsenoside.Ginsenoside biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural genes.Nonetheless,the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary metabolites.Squalene epoxidase(SQE)is a key enzyme in the mevalonic acid pathway,which affects the biosynthesis of secondary metabolites such as terpenoid.Using ginseng transcriptome,expression,and ginsenoside content databases,this study employed bioinformatic methods to systematically analyze the genes encoding SQE in ginseng.We first selected six PgSQE candidates that were closely involved in ginsenoside biosynthesis and then identified PgSQE08-01 to be highly associated with ginsenoside biosynthesis.Next,we constructed the overexpression vector pCAMBIA3301-PgSQE08-01 and the RNAi vector pART27-PgSQE08-01 and transformed ginseng adventitious roots using Agrobacterium rhizogenes,to obtain positive hairy-root clones.Thereafter,quantitative reverse transcriptionpolymerase chain reaction and high-performance liquid chromatography were used to determine the expression of relevant genes and ginsenoside content,respectively.Then,we focused on the function of PgSQE08-01 gene,which was noted to be involved in ginsenoside biosynthesis.Thus,these findings not only provided a molecular basis for the identification of important functional genes in ginseng but also enriched genetic resources for the biosynthesis of ginsenosides using synthetic biology.
文摘Identification of management practices that can improve soil health is critical to improving the sustainability of soybean [Glycine max (L.) Merr.] production. The objective of this study was to examine the long-term effects of continuous soybean, corn-soybean, and soybean-cotton rotations with chicken litter and cover crops (hairy vetch, wheat, fallow) on soil health parameters, including nutrient accumulation and soil organic matter dynamics under a split plot design. The depth intervals of soil sampling were 0 - 15, 15 - 30, 30 - 60, and 60 - 90 cm. Chicken litter resulted in 62.1% and 32.8% higher water extractable organic soil N content than fallow and wheat, respectively, in the surface 0 - 15 cm of soil only. However, there was no significant difference in 1-day Solvita respiration, water extractable organic C, C/N ratio, health score, moisture, earthworm, organic matter, pH, or CEC of soil among fallow, hairy vetch, chicken litter, and wheat regardless of soil depth. Unexpectedly, annual application of chicken litter at 4.4 Mg ha−1 as an N source or growing a winter-season cover crop such as hairy vetch or wheat for continuous 16 years did not significantly increase soil organic matter or water extractable organic soil C. Annual application of chicken litter at 4.4 metric tons (Mg) ha–1 for 16 years increased soil nitrate-nitrogen (NO3−-N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), and zinc (Zn) contents by 92%, 400%, 134%, 20%, 43%, 206%, and 430% in 0 - 15 cm depth compared with their initial soil values, respectively, extracted with Haney H3A-2 (2 g L–1 lithium citrate + 0.6 g L–1 citric acid + 0.4 g L–1 malic acid + 0.4 g L–1 oxalic acid) solution. The increases enhanced soil supply of these nutrients to following crops, but also increased the risks of losing them to the environment. Hairy vetch caused higher H3A extracted soil manganese (Mn) content than fallow and chicken litter in 0 - 60 cm. There was no significant difference in 1-day Solvita respiration, water extractable organic C and N, health score, moisture, organic matter, pH, CEC, or population of earthworm of soil among continuous soybean, corn-soybean, and soybean-cotton in any soil depth. Another major finding of this study was that continuous soybean exerted no adverse effect on soil health relative to the commonly used corn (Zea mays L.)-soybean and soybean-cotton (Gossypium hirsutum L.) rotations under no-tillage after 16 years. To mitigate the risks of nutrient runoff and leaching from long-term chicken litter application, we recommend reducing litter application rates and integrating cover crops into crop rotations to enhance nutrient cycling and reduce environmental impacts.
文摘In this paper,we investigate the gravitational quasi-normal modes(QNMs)of Horndeski hairy black holes.By using the ordinary finite element method,the relations between the QNMs and the parameters of black holes are minutely shown.Both the oscillating frequency and the decay rate increase with the increase of the scalar charge Q.While the oscillating frequency and the decay rate decrease with the black hole mass M increasing.And the quasi-period of the oscillation decreases when the angular momentum l of the perturbation field increases.However,the decay rate has barely changed for different values of 1.Thus,the numerical results indicate that Horndeski black holes are stable.
文摘The artemisinin accumulation in the hairy root cultures of Artemisia annua L. was enhanced via a treatment of three fungal elicitors separately ( Verticillium dahliae Kleb., Rhizopus stolonifer (Ehrenb. ex Fr.) Vuill and Colletotrichum dematium (Pers.) Grove). Among these three elicitors, V. dahliae had the highest inducing efficiency, but none of them manifests any noticeable effects on the cell growth of the hairy root cultures. The artemisinin content of the hairy root cultures treated with V. dahliae elicitor was 1.12 mg/g DW, which was 45% higher than the control (0.77 mg/g DW). The results showed that elicitation was dependent on the elicitor concentration, the incubation period and the physiological stage at which the hairy root cultures were treated. In addition, the authors found that for V. dahliae , the optimum concentration was 0.4 mg carbohydrate per millilitre medium, the strongest response of A. annua hairy root cultures to the elicitation was at the late exponential growth stage, and the highest artemisinin content of the hairy root cultures was on the 4th day post treatment.
基金Sponsored by Shanghai Rising Star Program, No .02QF14005
文摘The technological process of compact spinning and the compact procedure of fiber band in compact field are briefly illustrated. The motions of fiber band in compact field are discussed theoretically from which tilting angle of suction slot in profile tube, additional twists created by fiber band’s rotating around its own axis and ultimate twists in compact yarn are deduced accordingly. The existence of additional twists is also verified through experiments.
文摘Variations in leaf morphological characteristics have been extensively studied at both interand intraspecific levels although not explicitly on paper birch (Betula papyrifera Marsh). Paper birch populations might have considerable genotypic and leaf morphological variations that have allowed them to inhabit wide environmental gradients. In this study, we analyzed variations in leaf morphological characteristics in 23 paper birch populations collected across Canada and grown in a greenhouse. Furthermore, we examined whether the variations in leaf morphological characteristics observed were related to the climate of the population’s origin. We found significant genotypic differences in all leaf morphological characteristics (p < 0.05) measured among the birch populations. Thus, we expected that the morphological variations in birch might be related to natural diversity in birch populations due to environmental differences at habitat origin. Principal component analysis (PCA) reduced thirteen leaf morphological variables to five principal components (PC) explaining 84.74% of the total variance in the original data. PCs accumulated with specific leaf area, petiole and leaf width were positively related to latitudinal, longitudinal, and elevational gradients at the population’s origin. Unpredictably, these PCs were significantly negatively correlated to precipitation and aridity index at the origin. Thus, we analyzed if correlations within leaf morphological characteristics had supported the birch populations to acclimate and produce unpredictable relations with the environment of origin. Our results showed that the populations originated in limited precipitation (during growing season) had large leaf width and petiole size but low leaf hairs on adaxial surface. Thus, all these leaf morphological features provide a basis for the birch to reduce water loss from leaves and balance water use efficiency in reduced precipitation. Furthermore, the leaf characteristics measured may also include phenotypic plasticity of the birch as an acclimation to the environment as in the greenhouse.
