A new algorithm is proposed to determine the actual length and the number of the protruding fibres of yarn based on a combination of image acquisition technology. First, a yarn hairiness image is obtained by the serie...A new algorithm is proposed to determine the actual length and the number of the protruding fibres of yarn based on a combination of image acquisition technology. First, a yarn hairiness image is obtained by the series of image processing procedures that include grayscale transformation,skew correction,yarn binary image acquisition and yarn core binary image obtaining. Then,the hairiness is realized in single pixel width by the usage of thinning algorithm. Finally, a baseline of yarn core margin is obtained,and pixels that match 8-neighbor template correctly are found by row scanning in a certain area. From this,these pixels are judged and the real crossover points of yarn core margin and hairiness,i. e.,the starting points of hairiness,are gained. The real length of the protruding fibres is calculated by tracking hairiness from the starting point constantly.展开更多
Yam hairiness is a complex concept, which generally cannot be completely defined by a single figure. Hairiness can be considered as the fiber ends and loops standing out from the main compact yarn body. Uster hairines...Yam hairiness is a complex concept, which generally cannot be completely defined by a single figure. Hairiness can be considered as the fiber ends and loops standing out from the main compact yarn body. Uster hairiness system characterizes the hairiness by H value, i.e. the mean value of the total length of all hairs within one centimeter of yarn. The raw data HI are in fact realization of spatial process （hairiness spatial process -- HSP） and can be used for more complex evaluation of hairiness characteristics in the space and frequency domain. The main aim of this contribution is description of some tools for spatial characterization of yarn hairiness. The simple methods for complex characterization of lISP statistical behavior （stationarity, independence, linearity etc. ） are presented. The techniques based on the embedding dimension and correlation integral or long-range dependences evaluation are discussed. The selected methods are core of HYARN program in MATLAB. Application of this program for deeper characterization of artificial data and cotton type yam are shown.展开更多
Traditional ring spinning system is associated with the several limitations, one of which is yarn hairiness. The awareness has been vibrantly growing among the spinning mills for reducing staple yarn hairiness because...Traditional ring spinning system is associated with the several limitations, one of which is yarn hairiness. The awareness has been vibrantly growing among the spinning mills for reducing staple yarn hairiness because of its detrimental effects on the subsequent processes (i.e., weaving, knitting, dyeing etc.) and the quality of resultant textile products. As a result, modified form of ring frame has attracted a lot of attention among the researchers for making yarn with the lower level of hairiness value. Besides the raw material parameters and some processing variables, spinning triangle (ST) is the most critical region that has decisive influence on the distribution of fiber tensions and their spatial orientations and shapes in staple yarn body. Thus, using appropriate methods to control the spinning triangle geometry actively has been a vital issue in the field of yarn hairiness reduction. In this paper, it is attempted to make an overview on the spinning triangle based modifications of ring frame that have been designed and destined to reduce hairiness. However, agent aided ring spinning system has also been briefly discussed in this paper as well.展开更多
In order to improve the quality of yarn,a research was carried out at ring spinning.A modifiedtraveller has been used to reduce the yarn hairiness vastly.According to the statistical analysis ofthe data,it was found t...In order to improve the quality of yarn,a research was carried out at ring spinning.A modifiedtraveller has been used to reduce the yarn hairiness vastly.According to the statistical analysis ofthe data,it was found that the geometrical shape of travellers is the key factor affecting the yarnhairiness at ring spinning.展开更多
Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herb...Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herbaceous plants,with notable applications in species such as Arabidopsis(Yin et al.,2024),soybean(Zhang et al.,2024),rice(Zhang et al.,2020),and Chinese cabbage(Li et al.,2021).However,its application in fruit trees is limited.This is primarily due to their long growth cycles and lack of rapid,efficient,and stable transgenic systems,which severely hinders foundational research involving plant genetic transformation(Mei et al.,2024).Furthermore,for subtropical fruit trees,the presence of recalcitrant seeds adds an extra layer of difficulty to genetic transformation(Umarani et al.,2015),as most methods rely on seed germination as a basis for transformation.展开更多
Drought is a major environmental constraint that significantly affects seedling emergence,yield,and quality of Tartary buckwheat,thereby hindering the development of its industry.However,the molecular mechanisms under...Drought is a major environmental constraint that significantly affects seedling emergence,yield,and quality of Tartary buckwheat,thereby hindering the development of its industry.However,the molecular mechanisms underlying drought tolerance genes in Tartary buckwheat remain largely unexplored.Alcohol dehydrogenase(ADH),an essential plant protein,plays a crucial role in growth,development,and stress responses;however,its specific role in drought resistance remains unclear.This study identifies an ADH gene,FtADH1,using a membership function value of drought tolerance(MFVD)combined with a genome-wide association study(GWAS)and transcriptomic profiles that confer drought tolerance in Tartary buckwheat.Our findings demonstrated that the overexpression of FtADH1 in Arabidopsis and Tartary buckwheat hairy roots enhances drought tolerance by promoting root elongation and mitigating elevated levels of reactive oxygen species(ROS).Our findings demonstrate that FtADH1 can enhance drought tolerance in Tartary buckwheat and Arabidopsis.This study identifies FtADH1 as a new regulator of Tartary buckwheat’s ROS levels and stress responses,functioning by regulating protective enzyme activities at a high level to scavenge ROS and modulating root growth under drought stress.Further,it identifies proteins interacting with FtADH1 through a prokaryotic expression pull-down assay combined with mass spectrometry,revealing that FtADH1 interacts explicitly with the S-adenosyl-L-methionine(SAM)synthetase protein,FtSAMS1.Overexpression of FtSAMS1 enhances ADH enzymatic activity,leading to increased SAM content in overexpressing Tartary buckwheat hairy roots under water-deficit conditions.Additionally,overexpression of FtSAMS1 induces a drought-resistant phenotype in Arabidopsis and Tartary buckwheat hairy roots under drought stress,revealing the biological function of FtADH1.Evolutionary analysis indicates that ADH1 in Fagopyrum species has undergone significant evolutionary events,including duplication and purifying selection,which may contribute to functional diversification and adaptive advantages such as drought resistance in cultivated buckwheat.In summary,this study suggests that FtADH1 is a key contributor to drought tolerance,and its interaction with FtSAMS1 offers promising potential for developing drought-resistant varieties in Tartary buckwheat and its relative species.展开更多
Cannabis sativa is highly valued for its use in fiber production,medicine,and recreational products.Its secondary metabolites(SM)are renowned for their wide range of health benefits and psychoactive properties.While m...Cannabis sativa is highly valued for its use in fiber production,medicine,and recreational products.Its secondary metabolites(SM)are renowned for their wide range of health benefits and psychoactive properties.While much of the existing research has focused on cannabinoid production in the plant’s aerial parts,particularly the leaves and flowers,the root system remains understudied in terms of its SM profile.One promising in vitro approach for metabolite production involves the use of‘hairy roots(HRs)’.These roots mimic the phytochemical profile of native roots but grow more efficiently and yield higher quantities of metabolites.HRs are genetically altered root tissues that develop at the site of infection when Agrobacterium rhizogenes is introduced into wounded plant tissues.HRs cultures in Cannabis represent a breakthrough in plant metabolic engineering,offering potential for the controlled biosynthesis of cannabinoids and terpenoids.By utilising genome editing(GE)tools such as CRISPR-based tools,these cultures can produce novel bioactive compounds at an industrial scale.The use of elicitors enhances the production of SM by activating their biosynthetic pathways,further boosting yields.This system provides a sustainable alternative to conventional farming and chemical synthesis,addressing challenges such as pharmaceutical shortages,enhancing climate resilience,and promoting more resource-efficient biomanufacturing.Few studies have explored elicitor-induced HR cultures in Cannabis to enhance terpenoid production.This review highlights research on HRs for SM synthesis and introduces a platform that positions Cannabis as a leader in biomanufacturing and sustainable biotechnology,promoting advancements across the agricultural and pharmaceutical industries globally.展开更多
Tartary buckwheat(Fagopyrum tataricum),an underutilized pseudocereal,possesses significant nutritional and pharmaceutical properties and demonstrates resistance to drought and nutrient deficiency.However,this environm...Tartary buckwheat(Fagopyrum tataricum),an underutilized pseudocereal,possesses significant nutritional and pharmaceutical properties and demonstrates resistance to drought and nutrient deficiency.However,this environmentally sustainable crop exhibits sensitivity to salt stress,which can induce water loss,stomatal closure,impair photosynthesis and metabolism,and diminish yield and quality of Tartary buckwheat.Understanding the mechanisms of salt stress tolerance in buckwheat is therefore crucial.This study identified a locus containing 35 candidate genes on chromosome 2 that shows significant association with salt tolerance of Tartary buckwheat through genome-wide association analysis(GWAS).Transcriptome analysis demonstrated that the serine/threonine-protein kinase Aurora-3(FtAUR3)family gene exhibited upregulation in response to salt stress.A single nucleotide deletion in the FtAUR3 promoter results in elevated FtAUR3 expression and enhanced salt tolerance in Tartary buckwheat.Overexpression of FtAUR3 in buckwheat hairy roots promotes the accumulation of flavonoids,including rutin and cinnamic acid,while inducing the expression of flavonoid biosynthesis genes,such as PAL,C4H,F3H,and F3'H,under salt stress.Additionally,overexpression of FtAUR3 in Arabidopsis thaliana induced the expression of salt-resistant genes(salt-resistant genes(SOS1),AVP1,etc.)and enhanced salt tolerance compared to wild type plants.Under salt stress,FtAUR3 significantly enhances the levels of reactive oxygen species pathway components,including superoxide dismutase,catalase,and peroxidase,thereby improving plant salt tolerance.The study demonstrated that FtAUR3 interacts with the critical enzyme Ft GAPB in the reactive oxygen species(ROS)pathway,suggesting a potential mechanism through which FtAUR3 contributes to ROS signaling.These findings indicate that FtAUR3 plays a crucial positive role in Tartary buckwheat resistance against salt stress.展开更多
In this work,we apply tunneling formalism to analyze charged particles tunneling across a hairy black hole horizon.Such black hole solutions are essential for frameworks based on Horndeski's gravity theory.Applyin...In this work,we apply tunneling formalism to analyze charged particles tunneling across a hairy black hole horizon.Such black hole solutions are essential for frameworks based on Horndeski's gravity theory.Applying a semi-classical technique,we examine the tunneling of charged particles from a hairy black hole and derive the generic tunneling spectrum of released particles,ignoring self-gravitational and interaction.It is studied to ignore the back-reaction impact of the radiated particle on the hairy black hole.We analyze the properties of the black hole,such as temperature and entropy,under the influence of quantum gravity and also observe that the firstorder correction is present.We study tunneling radiation produced by a charged field equation in the presence of a generalized uncertainty effect.We modify the semi-classical technique by using the generalized uncertainty principle,the WKB approximation,and surface gravity.展开更多
The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop genetics.Within this system,single guide RNAs(sgRNAs)are instrumental in the precise cleavage of DNA double strands.However,the efficie...The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop genetics.Within this system,single guide RNAs(sgRNAs)are instrumental in the precise cleavage of DNA double strands.However,the efficiency of gene editing varies among sgRNAs,emphasizing the need to meticulously select target sites,especially in the context of Citrullus lanatus,a species notorious for its challenging and inefficient generation of transgenic plants through stable transformation.This study employed an Agrobacterium rhizogenes-mediated hairy root method to assess effective target sites for gene editing of ClCIPK17 across various Citrullus species.Hairy roots were successfully induced in different plant tissues at diverse growth stages of Citrullus lanatus,Citrullus mucosospermus,and Citrullus amarus.Employing a vector with two sgRNAs(sgRNA1 and sgRNA5)positioned within conserved regions of exon 1 and exon 5 of ClCIPK17 in the CRISPR/Cas9 system,targeted mutations were detected in 90.9%of accessions across the four Citrullus species.Notably,73.94%of all examined hairy roots exhibited mutations at the sgRNA1 site,while the sgRNA5 site showed no mutations.Among the 31 different mutation types identified at the sgRNA1 site,base deletion was the most prevalent.Using the sgRNA1 site of ClCIPK17,stable transgenic watermelon buds were obtained from explants,and the targeted mutations of the sgRNA1 site were confirmed.These findings underscore the viability of the hairy root transformation system in assessing the editing efficiency of sgRNA targets in diverse Citrullus species.展开更多
The artemisinin accumulation in the hairy root cultures of Artemisia annua L. was enhanced via a treatment of three fungal elicitors separately ( Verticillium dahliae Kleb., Rhizopus stolonifer (Ehrenb. ex ...The artemisinin accumulation in the hairy root cultures of Artemisia annua L. was enhanced via a treatment of three fungal elicitors separately ( Verticillium dahliae Kleb., Rhizopus stolonifer (Ehrenb. ex Fr.) Vuill and Colletotrichum dematium (Pers.) Grove). Among these three elicitors, V. dahliae had the highest inducing efficiency, but none of them manifests any noticeable effects on the cell growth of the hairy root cultures. The artemisinin content of the hairy root cultures treated with V. dahliae elicitor was 1.12 mg/g DW, which was 45% higher than the control (0.77 mg/g DW). The results showed that elicitation was dependent on the elicitor concentration, the incubation period and the physiological stage at which the hairy root cultures were treated. In addition, the authors found that for V. dahliae , the optimum concentration was 0.4 mg carbohydrate per millilitre medium, the strongest response of A. annua hairy root cultures to the elicitation was at the late exponential growth stage, and the highest artemisinin content of the hairy root cultures was on the 4th day post treatment.展开更多
The technological process of compact spinning and the compact procedure of fiber band in compact field are briefly illustrated. The motions of fiber band in compact field are discussed theoretically from which tilting...The technological process of compact spinning and the compact procedure of fiber band in compact field are briefly illustrated. The motions of fiber band in compact field are discussed theoretically from which tilting angle of suction slot in profile tube, additional twists created by fiber band’s rotating around its own axis and ultimate twists in compact yarn are deduced accordingly. The existence of additional twists is also verified through experiments.展开更多
Variations in leaf morphological characteristics have been extensively studied at both interand intraspecific levels although not explicitly on paper birch (Betula papyrifera Marsh). Paper birch populations might have...Variations in leaf morphological characteristics have been extensively studied at both interand intraspecific levels although not explicitly on paper birch (Betula papyrifera Marsh). Paper birch populations might have considerable genotypic and leaf morphological variations that have allowed them to inhabit wide environmental gradients. In this study, we analyzed variations in leaf morphological characteristics in 23 paper birch populations collected across Canada and grown in a greenhouse. Furthermore, we examined whether the variations in leaf morphological characteristics observed were related to the climate of the population’s origin. We found significant genotypic differences in all leaf morphological characteristics (p < 0.05) measured among the birch populations. Thus, we expected that the morphological variations in birch might be related to natural diversity in birch populations due to environmental differences at habitat origin. Principal component analysis (PCA) reduced thirteen leaf morphological variables to five principal components (PC) explaining 84.74% of the total variance in the original data. PCs accumulated with specific leaf area, petiole and leaf width were positively related to latitudinal, longitudinal, and elevational gradients at the population’s origin. Unpredictably, these PCs were significantly negatively correlated to precipitation and aridity index at the origin. Thus, we analyzed if correlations within leaf morphological characteristics had supported the birch populations to acclimate and produce unpredictable relations with the environment of origin. Our results showed that the populations originated in limited precipitation (during growing season) had large leaf width and petiole size but low leaf hairs on adaxial surface. Thus, all these leaf morphological features provide a basis for the birch to reduce water loss from leaves and balance water use efficiency in reduced precipitation. Furthermore, the leaf characteristics measured may also include phenotypic plasticity of the birch as an acclimation to the environment as in the greenhouse.展开更多
The compact spinning with pneumatic groove utilizes transverse air force and mechanical force of the pneumatic groove to condense fiber bundles to eliminate the spinning triangle.Range analysis and variance analysis o...The compact spinning with pneumatic groove utilizes transverse air force and mechanical force of the pneumatic groove to condense fiber bundles to eliminate the spinning triangle.Range analysis and variance analysis of orthogonal experiment were used to discover both important and general factors that influence the condensing effect of the compact yarn including negative pressure,traveler,roving moisture regain,and spinning tension.Results from this research have confirmed that the spinning tension is the most important factor affecting the condensing effect and the yarn quality;the negative pressure,traveler,and roving moisture regain are general factors.The optimal combination for producing least yarn hairiness,A3B2C1D1,is acquired,where the value of negative pressure A is -3.0 kPa,roving moisture regain B is 7.64%,traveler C is 20 #(yarn tex 20.8),and spinning tension D is 25 cN.展开更多
The effects of the blending ratio on the properties of Ramie/Polyester blended yarns are discussed in this paper. The experimental results show that the elongation of the yarn with the polyester content more than 40% ...The effects of the blending ratio on the properties of Ramie/Polyester blended yarns are discussed in this paper. The experimental results show that the elongation of the yarn with the polyester content more than 40% is better than that of others. There is a lowest tenacity of the yarn corresponding to the blending ratio of 50/50 or so. The other properties of the yarn, such as the evenness and hairiness, will be improved with the increasing of the polyester content.展开更多
Using the forearm test, the prickle of 26 commercially available light-weight worsted woven wool fabrics and 7 other fiber fabrics were studied under (24±1)℃ temperature and (65±5)% RH conditions. The surfa...Using the forearm test, the prickle of 26 commercially available light-weight worsted woven wool fabrics and 7 other fiber fabrics were studied under (24±1)℃ temperature and (65±5)% RH conditions. The surface fiber diameter of part of the wool fabrics was measured using a microscope. It was found that most of the light-weight worsted woven wool fabrics gave a prickle sensation under the above conditions. The prickle sensation was significantly correlated with the mean fiber diameter of the surface hairiness. It was also found that the prickle of the light-weight worsted woven wool fabrics was significantly correlated with the number of surface fibers which were coarser than 26 μm diameter.展开更多
The mechanism of filament pre-tension is put forward. The effect of filament pre-tension on Sirofil composite yam properties, such as hairiness and structural stability, are theoretically and experimentally studied. T...The mechanism of filament pre-tension is put forward. The effect of filament pre-tension on Sirofil composite yam properties, such as hairiness and structural stability, are theoretically and experimentally studied. The theoretical analysis agrees well with the experimental results. The present theory shows that suitable choice of filament pre-tension can enormously reduce hairiness and improve structural stability of Sirofil composite yarn.展开更多
By nature, pineapple fiber (PALF) obtained from pineapple leaf is a smooth, shiny and white natural cellulosic fiber. In current investigation of spinning technique for pineapple leaf fiber based on cotton spinning me...By nature, pineapple fiber (PALF) obtained from pineapple leaf is a smooth, shiny and white natural cellulosic fiber. In current investigation of spinning technique for pineapple leaf fiber based on cotton spinning method comparison of produced yarn properties has been reported. For one of the investigations of this study, the fibers were cut into staple length and various properties of fibers were analyzed. Data and results acquired from this illustrated that there is excellent spinnability on the fiber. Two separate researches were conducted with consideration to produce resultant yarn by spinning of PALF. Yarn derived by solo spinning technique of 100% PALF and PALF blending with polyester and cotton fibers through binary blending technique in equal proportion has been spun, then yarn count, tensile properties, yarn evenness, hairiness have been evaluated and analyzed. The results from numerical simulations analysis indicated that yarn obtained from the Pineapple leaf fiber (PALF) had great potential to be used in apparels.展开更多
基金National Natural Science Foundation of China(No.61301276)Xi’an Polytechnic University Young Scholar Backbone Supporting Plan,ChinaDiscipline Construction Funds of Xi’an Polytechnic University,China(No.107090811)
文摘A new algorithm is proposed to determine the actual length and the number of the protruding fibres of yarn based on a combination of image acquisition technology. First, a yarn hairiness image is obtained by the series of image processing procedures that include grayscale transformation,skew correction,yarn binary image acquisition and yarn core binary image obtaining. Then,the hairiness is realized in single pixel width by the usage of thinning algorithm. Finally, a baseline of yarn core margin is obtained,and pixels that match 8-neighbor template correctly are found by row scanning in a certain area. From this,these pixels are judged and the real crossover points of yarn core margin and hairiness,i. e.,the starting points of hairiness,are gained. The real length of the protruding fibres is calculated by tracking hairiness from the starting point constantly.
基金Supported by the research project"Textile center"of Czech Ministry of Education1M4674788501
文摘Yam hairiness is a complex concept, which generally cannot be completely defined by a single figure. Hairiness can be considered as the fiber ends and loops standing out from the main compact yarn body. Uster hairiness system characterizes the hairiness by H value, i.e. the mean value of the total length of all hairs within one centimeter of yarn. The raw data HI are in fact realization of spatial process （hairiness spatial process -- HSP） and can be used for more complex evaluation of hairiness characteristics in the space and frequency domain. The main aim of this contribution is description of some tools for spatial characterization of yarn hairiness. The simple methods for complex characterization of lISP statistical behavior （stationarity, independence, linearity etc. ） are presented. The techniques based on the embedding dimension and correlation integral or long-range dependences evaluation are discussed. The selected methods are core of HYARN program in MATLAB. Application of this program for deeper characterization of artificial data and cotton type yam are shown.
文摘Traditional ring spinning system is associated with the several limitations, one of which is yarn hairiness. The awareness has been vibrantly growing among the spinning mills for reducing staple yarn hairiness because of its detrimental effects on the subsequent processes (i.e., weaving, knitting, dyeing etc.) and the quality of resultant textile products. As a result, modified form of ring frame has attracted a lot of attention among the researchers for making yarn with the lower level of hairiness value. Besides the raw material parameters and some processing variables, spinning triangle (ST) is the most critical region that has decisive influence on the distribution of fiber tensions and their spatial orientations and shapes in staple yarn body. Thus, using appropriate methods to control the spinning triangle geometry actively has been a vital issue in the field of yarn hairiness reduction. In this paper, it is attempted to make an overview on the spinning triangle based modifications of ring frame that have been designed and destined to reduce hairiness. However, agent aided ring spinning system has also been briefly discussed in this paper as well.
文摘In order to improve the quality of yarn,a research was carried out at ring spinning.A modifiedtraveller has been used to reduce the yarn hairiness vastly.According to the statistical analysis ofthe data,it was found that the geometrical shape of travellers is the key factor affecting the yarnhairiness at ring spinning.
基金funded by the Key-Area Research and Development Program of Guangdong Province(Grant No.2022B0202070002)the Guangxi Science and Technology Major Program(Grant No.GuikeAA23023007-2)+1 种基金the Guangdong Province Modern Agricultural Industry Technology System Innovation Team Construction Project(2024CXTD19)Guangdong Basic and Applied Basic Research Foundation(Grant No.2023A1515010303)。
文摘Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herbaceous plants,with notable applications in species such as Arabidopsis(Yin et al.,2024),soybean(Zhang et al.,2024),rice(Zhang et al.,2020),and Chinese cabbage(Li et al.,2021).However,its application in fruit trees is limited.This is primarily due to their long growth cycles and lack of rapid,efficient,and stable transgenic systems,which severely hinders foundational research involving plant genetic transformation(Mei et al.,2024).Furthermore,for subtropical fruit trees,the presence of recalcitrant seeds adds an extra layer of difficulty to genetic transformation(Umarani et al.,2015),as most methods rely on seed germination as a basis for transformation.
基金supported by the National Natural Science Foundation of China(32372045)the Key Laboratory of Molecular Breeding for Grain and Oil Crops in Guizhou Province,China(Qiankehezhongyindi(2023)008)the Key Laboratory of Functional Agriculture of Guizhou Provincial Higher Education Institutions,China(Qianjiaoji(2023)007).
文摘Drought is a major environmental constraint that significantly affects seedling emergence,yield,and quality of Tartary buckwheat,thereby hindering the development of its industry.However,the molecular mechanisms underlying drought tolerance genes in Tartary buckwheat remain largely unexplored.Alcohol dehydrogenase(ADH),an essential plant protein,plays a crucial role in growth,development,and stress responses;however,its specific role in drought resistance remains unclear.This study identifies an ADH gene,FtADH1,using a membership function value of drought tolerance(MFVD)combined with a genome-wide association study(GWAS)and transcriptomic profiles that confer drought tolerance in Tartary buckwheat.Our findings demonstrated that the overexpression of FtADH1 in Arabidopsis and Tartary buckwheat hairy roots enhances drought tolerance by promoting root elongation and mitigating elevated levels of reactive oxygen species(ROS).Our findings demonstrate that FtADH1 can enhance drought tolerance in Tartary buckwheat and Arabidopsis.This study identifies FtADH1 as a new regulator of Tartary buckwheat’s ROS levels and stress responses,functioning by regulating protective enzyme activities at a high level to scavenge ROS and modulating root growth under drought stress.Further,it identifies proteins interacting with FtADH1 through a prokaryotic expression pull-down assay combined with mass spectrometry,revealing that FtADH1 interacts explicitly with the S-adenosyl-L-methionine(SAM)synthetase protein,FtSAMS1.Overexpression of FtSAMS1 enhances ADH enzymatic activity,leading to increased SAM content in overexpressing Tartary buckwheat hairy roots under water-deficit conditions.Additionally,overexpression of FtSAMS1 induces a drought-resistant phenotype in Arabidopsis and Tartary buckwheat hairy roots under drought stress,revealing the biological function of FtADH1.Evolutionary analysis indicates that ADH1 in Fagopyrum species has undergone significant evolutionary events,including duplication and purifying selection,which may contribute to functional diversification and adaptive advantages such as drought resistance in cultivated buckwheat.In summary,this study suggests that FtADH1 is a key contributor to drought tolerance,and its interaction with FtSAMS1 offers promising potential for developing drought-resistant varieties in Tartary buckwheat and its relative species.
基金supported by a research grant of Gyeongsangbuk-do(No.GBHEMP202504),Republic of Korea.
文摘Cannabis sativa is highly valued for its use in fiber production,medicine,and recreational products.Its secondary metabolites(SM)are renowned for their wide range of health benefits and psychoactive properties.While much of the existing research has focused on cannabinoid production in the plant’s aerial parts,particularly the leaves and flowers,the root system remains understudied in terms of its SM profile.One promising in vitro approach for metabolite production involves the use of‘hairy roots(HRs)’.These roots mimic the phytochemical profile of native roots but grow more efficiently and yield higher quantities of metabolites.HRs are genetically altered root tissues that develop at the site of infection when Agrobacterium rhizogenes is introduced into wounded plant tissues.HRs cultures in Cannabis represent a breakthrough in plant metabolic engineering,offering potential for the controlled biosynthesis of cannabinoids and terpenoids.By utilising genome editing(GE)tools such as CRISPR-based tools,these cultures can produce novel bioactive compounds at an industrial scale.The use of elicitors enhances the production of SM by activating their biosynthetic pathways,further boosting yields.This system provides a sustainable alternative to conventional farming and chemical synthesis,addressing challenges such as pharmaceutical shortages,enhancing climate resilience,and promoting more resource-efficient biomanufacturing.Few studies have explored elicitor-induced HR cultures in Cannabis to enhance terpenoid production.This review highlights research on HRs for SM synthesis and introduces a platform that positions Cannabis as a leader in biomanufacturing and sustainable biotechnology,promoting advancements across the agricultural and pharmaceutical industries globally.
基金supported by the Asian Cooperation Fund Project(102125241610090000029)the National Key R&D Program of China(2019YFD1001300 and 2019YFD1001305)+1 种基金the Natural Science Foundation of Hebei Province,China(C2023204097)the National Natural Science Foundation of China(31560578)。
文摘Tartary buckwheat(Fagopyrum tataricum),an underutilized pseudocereal,possesses significant nutritional and pharmaceutical properties and demonstrates resistance to drought and nutrient deficiency.However,this environmentally sustainable crop exhibits sensitivity to salt stress,which can induce water loss,stomatal closure,impair photosynthesis and metabolism,and diminish yield and quality of Tartary buckwheat.Understanding the mechanisms of salt stress tolerance in buckwheat is therefore crucial.This study identified a locus containing 35 candidate genes on chromosome 2 that shows significant association with salt tolerance of Tartary buckwheat through genome-wide association analysis(GWAS).Transcriptome analysis demonstrated that the serine/threonine-protein kinase Aurora-3(FtAUR3)family gene exhibited upregulation in response to salt stress.A single nucleotide deletion in the FtAUR3 promoter results in elevated FtAUR3 expression and enhanced salt tolerance in Tartary buckwheat.Overexpression of FtAUR3 in buckwheat hairy roots promotes the accumulation of flavonoids,including rutin and cinnamic acid,while inducing the expression of flavonoid biosynthesis genes,such as PAL,C4H,F3H,and F3'H,under salt stress.Additionally,overexpression of FtAUR3 in Arabidopsis thaliana induced the expression of salt-resistant genes(salt-resistant genes(SOS1),AVP1,etc.)and enhanced salt tolerance compared to wild type plants.Under salt stress,FtAUR3 significantly enhances the levels of reactive oxygen species pathway components,including superoxide dismutase,catalase,and peroxidase,thereby improving plant salt tolerance.The study demonstrated that FtAUR3 interacts with the critical enzyme Ft GAPB in the reactive oxygen species(ROS)pathway,suggesting a potential mechanism through which FtAUR3 contributes to ROS signaling.These findings indicate that FtAUR3 plays a crucial positive role in Tartary buckwheat resistance against salt stress.
基金funded by the National Natural Science Foundation of China under Grant No.11975145。
文摘In this work,we apply tunneling formalism to analyze charged particles tunneling across a hairy black hole horizon.Such black hole solutions are essential for frameworks based on Horndeski's gravity theory.Applying a semi-classical technique,we examine the tunneling of charged particles from a hairy black hole and derive the generic tunneling spectrum of released particles,ignoring self-gravitational and interaction.It is studied to ignore the back-reaction impact of the radiated particle on the hairy black hole.We analyze the properties of the black hole,such as temperature and entropy,under the influence of quantum gravity and also observe that the firstorder correction is present.We study tunneling radiation produced by a charged field equation in the presence of a generalized uncertainty effect.We modify the semi-classical technique by using the generalized uncertainty principle,the WKB approximation,and surface gravity.
基金supported by the Basic Research Funds of Chinese Academy of Agricultural Sciences(Grant No.1610192023201)the National Key Research and Development Program of China(Grant No.SQ2023YFE0201239)+3 种基金China Agriculture Research System of MOF and MARA(Grant No.CARS-25-03)the Major Science and Technology Projects of Henan Province(Grant No.221100110400)the Funding of Joint Research on Agricultural Variety Improvement of Henan Province(Grant No.2022010503)the Agricultural Science and Technology Innovation Program(Grant No.CAAS-ASTIP-2021-ZFRI)。
文摘The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop genetics.Within this system,single guide RNAs(sgRNAs)are instrumental in the precise cleavage of DNA double strands.However,the efficiency of gene editing varies among sgRNAs,emphasizing the need to meticulously select target sites,especially in the context of Citrullus lanatus,a species notorious for its challenging and inefficient generation of transgenic plants through stable transformation.This study employed an Agrobacterium rhizogenes-mediated hairy root method to assess effective target sites for gene editing of ClCIPK17 across various Citrullus species.Hairy roots were successfully induced in different plant tissues at diverse growth stages of Citrullus lanatus,Citrullus mucosospermus,and Citrullus amarus.Employing a vector with two sgRNAs(sgRNA1 and sgRNA5)positioned within conserved regions of exon 1 and exon 5 of ClCIPK17 in the CRISPR/Cas9 system,targeted mutations were detected in 90.9%of accessions across the four Citrullus species.Notably,73.94%of all examined hairy roots exhibited mutations at the sgRNA1 site,while the sgRNA5 site showed no mutations.Among the 31 different mutation types identified at the sgRNA1 site,base deletion was the most prevalent.Using the sgRNA1 site of ClCIPK17,stable transgenic watermelon buds were obtained from explants,and the targeted mutations of the sgRNA1 site were confirmed.These findings underscore the viability of the hairy root transformation system in assessing the editing efficiency of sgRNA targets in diverse Citrullus species.
文摘The artemisinin accumulation in the hairy root cultures of Artemisia annua L. was enhanced via a treatment of three fungal elicitors separately ( Verticillium dahliae Kleb., Rhizopus stolonifer (Ehrenb. ex Fr.) Vuill and Colletotrichum dematium (Pers.) Grove). Among these three elicitors, V. dahliae had the highest inducing efficiency, but none of them manifests any noticeable effects on the cell growth of the hairy root cultures. The artemisinin content of the hairy root cultures treated with V. dahliae elicitor was 1.12 mg/g DW, which was 45% higher than the control (0.77 mg/g DW). The results showed that elicitation was dependent on the elicitor concentration, the incubation period and the physiological stage at which the hairy root cultures were treated. In addition, the authors found that for V. dahliae , the optimum concentration was 0.4 mg carbohydrate per millilitre medium, the strongest response of A. annua hairy root cultures to the elicitation was at the late exponential growth stage, and the highest artemisinin content of the hairy root cultures was on the 4th day post treatment.
基金Sponsored by Shanghai Rising Star Program, No .02QF14005
文摘The technological process of compact spinning and the compact procedure of fiber band in compact field are briefly illustrated. The motions of fiber band in compact field are discussed theoretically from which tilting angle of suction slot in profile tube, additional twists created by fiber band’s rotating around its own axis and ultimate twists in compact yarn are deduced accordingly. The existence of additional twists is also verified through experiments.
文摘Variations in leaf morphological characteristics have been extensively studied at both interand intraspecific levels although not explicitly on paper birch (Betula papyrifera Marsh). Paper birch populations might have considerable genotypic and leaf morphological variations that have allowed them to inhabit wide environmental gradients. In this study, we analyzed variations in leaf morphological characteristics in 23 paper birch populations collected across Canada and grown in a greenhouse. Furthermore, we examined whether the variations in leaf morphological characteristics observed were related to the climate of the population’s origin. We found significant genotypic differences in all leaf morphological characteristics (p < 0.05) measured among the birch populations. Thus, we expected that the morphological variations in birch might be related to natural diversity in birch populations due to environmental differences at habitat origin. Principal component analysis (PCA) reduced thirteen leaf morphological variables to five principal components (PC) explaining 84.74% of the total variance in the original data. PCs accumulated with specific leaf area, petiole and leaf width were positively related to latitudinal, longitudinal, and elevational gradients at the population’s origin. Unpredictably, these PCs were significantly negatively correlated to precipitation and aridity index at the origin. Thus, we analyzed if correlations within leaf morphological characteristics had supported the birch populations to acclimate and produce unpredictable relations with the environment of origin. Our results showed that the populations originated in limited precipitation (during growing season) had large leaf width and petiole size but low leaf hairs on adaxial surface. Thus, all these leaf morphological features provide a basis for the birch to reduce water loss from leaves and balance water use efficiency in reduced precipitation. Furthermore, the leaf characteristics measured may also include phenotypic plasticity of the birch as an acclimation to the environment as in the greenhouse.
基金National Key Technologies R&D Program of China during the 11th Five-Year Plan Period(No.2007BAE41B04)
文摘The compact spinning with pneumatic groove utilizes transverse air force and mechanical force of the pneumatic groove to condense fiber bundles to eliminate the spinning triangle.Range analysis and variance analysis of orthogonal experiment were used to discover both important and general factors that influence the condensing effect of the compact yarn including negative pressure,traveler,roving moisture regain,and spinning tension.Results from this research have confirmed that the spinning tension is the most important factor affecting the condensing effect and the yarn quality;the negative pressure,traveler,and roving moisture regain are general factors.The optimal combination for producing least yarn hairiness,A3B2C1D1,is acquired,where the value of negative pressure A is -3.0 kPa,roving moisture regain B is 7.64%,traveler C is 20 #(yarn tex 20.8),and spinning tension D is 25 cN.
文摘The effects of the blending ratio on the properties of Ramie/Polyester blended yarns are discussed in this paper. The experimental results show that the elongation of the yarn with the polyester content more than 40% is better than that of others. There is a lowest tenacity of the yarn corresponding to the blending ratio of 50/50 or so. The other properties of the yarn, such as the evenness and hairiness, will be improved with the increasing of the polyester content.
文摘Using the forearm test, the prickle of 26 commercially available light-weight worsted woven wool fabrics and 7 other fiber fabrics were studied under (24±1)℃ temperature and (65±5)% RH conditions. The surface fiber diameter of part of the wool fabrics was measured using a microscope. It was found that most of the light-weight worsted woven wool fabrics gave a prickle sensation under the above conditions. The prickle sensation was significantly correlated with the mean fiber diameter of the surface hairiness. It was also found that the prickle of the light-weight worsted woven wool fabrics was significantly correlated with the number of surface fibers which were coarser than 26 μm diameter.
基金National Natural Science Foundation of China (No.10572038)the Program for New Century Excellent Talents in University .
文摘The mechanism of filament pre-tension is put forward. The effect of filament pre-tension on Sirofil composite yam properties, such as hairiness and structural stability, are theoretically and experimentally studied. The theoretical analysis agrees well with the experimental results. The present theory shows that suitable choice of filament pre-tension can enormously reduce hairiness and improve structural stability of Sirofil composite yarn.
文摘By nature, pineapple fiber (PALF) obtained from pineapple leaf is a smooth, shiny and white natural cellulosic fiber. In current investigation of spinning technique for pineapple leaf fiber based on cotton spinning method comparison of produced yarn properties has been reported. For one of the investigations of this study, the fibers were cut into staple length and various properties of fibers were analyzed. Data and results acquired from this illustrated that there is excellent spinnability on the fiber. Two separate researches were conducted with consideration to produce resultant yarn by spinning of PALF. Yarn derived by solo spinning technique of 100% PALF and PALF blending with polyester and cotton fibers through binary blending technique in equal proportion has been spun, then yarn count, tensile properties, yarn evenness, hairiness have been evaluated and analyzed. The results from numerical simulations analysis indicated that yarn obtained from the Pineapple leaf fiber (PALF) had great potential to be used in apparels.
