BACKGROUND Exosome-based therapies represent a promising approach for hair regeneration.Unlike conventional treatments such as minoxidil and finasteride,exosomes deliver bioactive cargo that can stimulate dermal papil...BACKGROUND Exosome-based therapies represent a promising approach for hair regeneration.Unlike conventional treatments such as minoxidil and finasteride,exosomes deliver bioactive cargo that can stimulate dermal papilla cells,enhance angiogenesis,and modulate inflammatory pathways.However,variability in exosome sources,isolation techniques,and dosing protocols limits their clinical translation.AIM To synthesize findings from in vitro,preclinical and clinical studies,and to evaluate the efficacy,mechanisms,and challenges associated with exosome-based hair restoration therapies.METHODS A literature search was conducted using multiple databases(PubMed/Medline,Embase,Scopus,and Web of Science)employing terms for exosomes and hair regeneration for articles published in English to February 2025,following Preferred Reporting Items for Systematic Reviews and Meta-analyses guidelines.RESULTS A total of 27 studies(three in vitro,three pre-clinical,18 with both in vitro and preclinical component and three clinical)met the pre-defined search and inclusion criteria and were included in this review.CONCLUSION Exosome-based therapies hold immense promise for hair regeneration by leveraging their ability to modulate key signaling pathways and enhance hair follicle regeneration.While in vitro and preclinical studies demonstrate consistent efficacy across diverse exosome sources,methodological heterogeneity and a limited number of clinical studies warrant further clinical research to realize their full clinical potential for hair regeneration.展开更多
A hair tonic containing 1.0%Arctium lappa root extract(ALRE)was evaluated for its efficacy and tolerability in Chinese consumers.ALRE was selected based on its ability to promote Collagen Type XVII Alpha 1(COL17A1)syn...A hair tonic containing 1.0%Arctium lappa root extract(ALRE)was evaluated for its efficacy and tolerability in Chinese consumers.ALRE was selected based on its ability to promote Collagen Type XVII Alpha 1(COL17A1)synthesis,along with other active ingredients targeting scalp health and follicular regeneration.In vitro assays confirmed that ALRE significantly enhanced COL17A1 expression.A 28-day clinical trial involving Chinese participants demonstrated that the tonic reduced hair loss by 37.61%and increased local hair density by 26.63%,with no reported adverse effects.These findings validate the product’s effectiveness in a distinct consumer population and highlight the importance of integrating mechanistic insights with clinical validation.Further research should explore long-term efficacy and demographic-specific responses to optimize its application.展开更多
The anti-hair loss mechanism of Aquilaria sinensis leaf extract(ASE)has been studied by using metabolomics and network pharmacology.Metabolomics was utilized to comprehensively identify the active constituents of ASE,...The anti-hair loss mechanism of Aquilaria sinensis leaf extract(ASE)has been studied by using metabolomics and network pharmacology.Metabolomics was utilized to comprehensively identify the active constituents of ASE,and the network pharmacology was used to elucidate their anti-hair loss mechanism,which was verified by molecular docking technology.572 active compounds were identified from the ASE by metabolomics methods,where there are 1447 corresponding targets and 492 targets related to hair loss,totaling 88 targets.20 core active substances were identified by constructing a network between common targets and active substances,which include vanillic acid,chorionic acid,caffeic acid and apigenin.The five key targets of TNF,TP53,IL6,PPARG,and EGFR were screened out by the PPI network analysis on 88 common targets.The GO and KEGG pathway enrichment analysis showed that the inflammation,hormone balance,cell growth,proliferation,apoptosis,and oxidative stress are involved.Molecular docking studies have confirmed the high binding affinity between core active compounds and key targets.The drug similarity assessment on these core compounds suggested that they have the potential to be used as potential hair loss treatment drugs.This study elucidates the complex molecular mechanism of ASE in treating hair loss,and provides a reference for the future applications in hair care products.展开更多
Objective This study aimed to comprehensively investigate the potential protective effects and underlying mechanisms of taurine against dihydrotestosterone(DHT)-induced androgenetic alopecia(AGA)in male C57BL/6 mice,w...Objective This study aimed to comprehensively investigate the potential protective effects and underlying mechanisms of taurine against dihydrotestosterone(DHT)-induced androgenetic alopecia(AGA)in male C57BL/6 mice,with a focus on hair follicle cycle modulation,cellular proliferation/apoptosis,and key related signaling pathways.Methods Six-week-old female C57BL/6 mice were initially used to assess the hair growth-promoting potential of taurine.After acclimatization,they were randomly assigned to three groups(n=8):control(regular drinking water),taurine(drinking water containing 1%taurine),and minoxidil(topical 2%minoxidil,positive control).For the AGA study,male C57BL/6 mice were randomly divided into five groups(n=8):control(physiological saline),DHT(model group,1 mg/d DHT),DHT+low-dose taurine(1 mg/d DHT+2 mg/d taurine),DHT+high-dose taurine(1 mg/d DHT+10 mg/d taurine),and DHT+minoxidil(positive control,1 mg/d DHT+topical 2%minoxidil).One day before treatment initiation,dorsal hair was shaved with scissors,and residual hair was removed using a depilatory cream.DHT and taurine were administered via daily intraperitoneal injection.Hair regrowth was assessed by photographing the depilated area at regular intervals and quantified using a four-point grading system(0-3).Dorsal skin samples were collected on day 14 for histological analysis(H&E staining),immunofluorescence staining(Ki67 for proliferation,TUNEL for apoptosis),ELISA(DHT quantification),RT-qPCR,and Western blot analysis to evaluate the expression of key genes and proteins(androgen receptor(AR),transforming growth factor(TGF)‑β1,TGF‑β2,Dickkopf-1(DKK1)).Results In female mice,taurine supplementation significantly accelerated hair growth,with effects comparable to minoxidil.This was evidenced by an earlier transition from pink(telogen)to black(anagen)skin and increased hair growth scores.Histological analysis showed that taurine increased hair follicle count and dermal thickness.Immunofluorescence confirmed enhanced keratinocyte proliferation in the hair matrix.In the DHTinduced AGA model,DHT significantly extended the telogen phase,inhibited hair growth,increased skin DHT content,and induced hair follicle miniaturization.Taurine treatment,particularly at the high dose,effectively counteracted these effects:it promoted the telogen-to-anagen transition and improved hair growth scores.Histomorphometric analysis showed that taurine significantly restored DHT-induced reductions in dermal thickness,hair follicle count,hair bulb depth,and follicle size.Taurine treatment also reduced apoptosis and promoted the proliferation of hair follicle cells,as demonstrated by Ki67 and TUNEL assays.Crucially,RT-qPCR and Western blot analyses revealed that DHT significantly up-regulated the expression of AR,TGF‑β1,TGF‑β2,and DKK1 at both mRNA and protein levels in dorsal skin.Taurine administration markedly down-regulated the expression of these pathogenic factors,bringing them closer to the levels observed in the control group.Conclusion Taurine demonstrates significant efficacy in alleviating DHT-induced AGA in male C57BL/6 mice.Its protective effects are mediated through multi-faceted mechanisms.(1)Promoting hair follicle cycle progression:it accelerates the transition from telogen to anagen,counteracting DHT-induced prolongation of the telogen phase.(2)Modulating cellular dynamics:it stimulates the proliferation of hair matrix keratinocytes and reduces DHT-induced apoptosis within hair follicle cells.(3)Suppressing androgen-driven pathogenic pathways:it downregulates the expression of critical molecules in the AGA pathway,including AR,the cytokines TGF-β1 and TGF-β2,and the Wnt pathway inhibitor DKK1.Given its favorable safety profile and multi-targeted action,taurine emerges as a promising novel therapeutic candidate or adjunct for treating AGA.Further investigation into its clinical potential and precise molecular mechanisms is warranted.This study provides a robust preclinical foundation for considering taurine supplementation or topical application in hair loss management strategies.展开更多
Hair coloring has emerged as an integral part of the cosmetic industry,particularly in response to the increasing global aging phenomenon.The natural melanin analog,polydopamine(PDA),has garnered considerable attentio...Hair coloring has emerged as an integral part of the cosmetic industry,particularly in response to the increasing global aging phenomenon.The natural melanin analog,polydopamine(PDA),has garnered considerable attention as an eco-friendly hair dye,and several kinds of polymerization ways of dopamine(DA)have been proposed including alkali catalysis,metal ion catalysis,strong oxidants,and enzyme-mediated oxidation reactions and polymerizations.Yet the controllability of polymerization and potential toxicity of involved metal ions are still in question.Inspired by the photoprotective mechanism in human skin,we have developed the melanin-inspired hair dyeing strategy that allowed for the in situ oxidative polymerization of DA under ultraviolet(UV)light.This polymerization was triggered by photobase generators(PBGs),a class of compounds that produced organic bases upon UV and sunlight irradiation.The resulting hair showed an adjustable color from light brown to black by tuning the ratio of DA and PBG(DA@PBG),the concentration of DA,and light exposure time.The dyed hairs showed excellent washing resistance and superior anti-static properties.Furthermore,Hair Color Spray DA@PBG also demonstrated a desirable hair dyeing effect and excellent biosecurity by simply spraying it on the hair under sunlight.This novel sunlight-induced method provided a new direction towards the preparation of natural hair dyes and could promote the development of green and safe hair dyes in colorful and brilliant artistic-grade hair coloring.展开更多
The genetic regulation of hair density in animals remains poorly understood.The Dazu black goat,characterized by its black coarse hair and white skin,provides a unique model for dissecting coarse hair density(CHD).Usi...The genetic regulation of hair density in animals remains poorly understood.The Dazu black goat,characterized by its black coarse hair and white skin,provides a unique model for dissecting coarse hair density(CHD).Using high-resolution micro-camera imaging,this study analyzed 905 skin images,33 skin transcriptomes,272 whole-genome sequences,and 182 downloaded transcriptomes.Morphological assessment from juvenile to adult stages revealed the thickening of hair shafts accompanied by a progressive decline in density,largely attributable to rapid surface expansion of the trunk skin.Transcriptomic comparison between high-and low-CHD individuals identified 572 differentially expressed genes(DEGs).A genome-wide association study detected 25 significant single nucleotide polymorphisms(P<9.07e-8)and mapped 48 annotated genes,with the most prominent association signal located near GJA1 on chr9.15931585-18621011.Literature review and Venn analysis highlighted six genes(GJA1,GPRC5D,CD1D,CD207,TFAM,and CXCL12)with documented roles in skin and hair biology,and three genes(GJA1,GPRC5D,and ATP6V1B1)overlapped with DEGs.Multiple-tissue transcriptomic profiling,western blotting,immunohistochemical staining,and skin single-cell RNA sequencing confirmed that GJA1 and GPRC5D were highly and specifically expressed in skin,particularly within hair follicles.Expression was localized predominantly to follicular stem cells and dermal papilla cells,suggesting a significant role in folliculogenesis and structural maintenance.Cross-validation using four public datasets further demonstrated positive correlations between GJA1 and GPRC5D expression and hair follicle density.The innovative micro-camera application allowed the elucidation of spatiotemporal patterns and genes associated with CHD,thereby addressing a significant knowledge gap in animal hair density.展开更多
Although cisplatin is a widely used chemotherapeutic agent,it is severely toxic and causes irreversible hearing loss,restricting its application in clinical settings.This study aimed to determine the molecular mechani...Although cisplatin is a widely used chemotherapeutic agent,it is severely toxic and causes irreversible hearing loss,restricting its application in clinical settings.This study aimed to determine the molecular mechanism underlying cisplatin-induced ototoxicity.Here,we established in vitro and in vivo ototoxicity models of cisplatininduced hair cell loss,and our results showed that reducing STING levels decreased inflammatory factor expression and hair cell death.In addition,we found that cisplatin-induced mitochondrial dysfunction was accompanied by cytosolic DNA,which may act as a critical linker between the cyclic GMP-AMP synthesis−stimulator of interferon genes(cGASSTING)pathway and the pathogenesis of cisplatin-induced hearing loss.H-151,a specific inhibitor of STING,reduced hair cell damage and ameliorated the hearing loss caused by cisplatin in vivo.This study underscores the role of cGASSTING in cisplatin ototoxicity and presents H-151 as a promising therapeutic for hearing loss.展开更多
The androgenetic alopecia(AGA)is the most prevalent clinical manifestation of hair loss,believed to be associated with excessive dihydrotestosterone(DHT)caused by typeⅡ5α-reductase(5αR2).The utilization of oral fin...The androgenetic alopecia(AGA)is the most prevalent clinical manifestation of hair loss,believed to be associated with excessive dihydrotestosterone(DHT)caused by typeⅡ5α-reductase(5αR2).The utilization of oral finasteride(FNS),which selectively inhibits 5αR2,is frequently constrained by its adverse effects.Topical FNS formulations can mitigate adverse effects but often exhibit limited dermal permeability.Nanocarriers show great potential in augmenting the cutaneous permeation of loaded FNS due to their inherent properties of selective accumulation within the hair follicles(HFs).In this study,hollow mesoporous silica nanoparticles(HMSN)with varying sizes were utilized as the nanocarriers for FNS,following mixing with the Carbopol hydrogel(F@H/Gel)for direct topical application.Specifically,the influence of size on the targeted delivery of FNS to HFs,and its enhanced therapeutic efficacy for the AGA mice model was evaluated.Results showed that the HMSN,with a diameter of approximately 300 nm,exhibited significant enhancement in FNS retention within skin and HFs,as well as remarkably accelerated hair regrowth on an AGA mouse model.In conclusion,this FNS topical formulation has proved to be a viable approach in offering a secure and efficient treatment modality for AGA.展开更多
Objective:To evaluate nurses’levels of knowledge and practice regarding preoperative hair removal and prevention of surgical site infection(SSI).Methods:The descriptive and cross-sectional research was conducted betw...Objective:To evaluate nurses’levels of knowledge and practice regarding preoperative hair removal and prevention of surgical site infection(SSI).Methods:The descriptive and cross-sectional research was conducted between February 2023 and May 2023 in a public hospital in northern Turkey with 123 nurses who agreed to participate in the study.Results:The results showed that 64.2%of the nurses thought that preoperative hair removal should be done by shaving with a razor,74%thought that hair removal should be performed the night before the operation,and 70.7%thought that the patient/patient’s relative should perform preoperative hair removal.Conclusions:This study found that nurses’levels of knowledge and practice regarding preoperative hair removal and prevention of SSI were not at the desired level.Following current resources and improving their levels of knowledge and practices are considered to be important for nurses.展开更多
Noise-induced hearing loss is a worldwide public health issue that is characterized by temporary or permanent changes in hearing sensitivity.This condition is closely linked to inflammatory responses,and interventions...Noise-induced hearing loss is a worldwide public health issue that is characterized by temporary or permanent changes in hearing sensitivity.This condition is closely linked to inflammatory responses,and interventions targeting the inflammatory gene tumor necrosis factoralpha(TNFα)are known to mitigate cochlear noise damage.TNFα-induced proteins(TNFAIPs)are a family of translucent acidic proteins,and TNFAIP6 has a notable association with inflammatory responses.To date,there have been few reports on TNFAIP6 levels in the inner ear.To elucidate the precise mechanism,we generated transgenic mouse models with conditional knockout of Tnfaip6(Tnfaip6 cKO).Evaluation of hair cell morphology and function revealed no significant differences in hair cell numbers or ribbon synapses between Tnfaip6 cKO and wild-type mice.Moreover,there were no notable variations in hair cell numbers or hearing function in noisy environments.Our results indicate that Tnfaip6 does not have a substantial impact on the auditory system.展开更多
The association between sugar-sweetened beverages(SSBs)intake and hair loss condition might give scientific support for better dietary practices to prevent hair loss.We aimed to explore the association of SSBs intake ...The association between sugar-sweetened beverages(SSBs)intake and hair loss condition might give scientific support for better dietary practices to prevent hair loss.We aimed to explore the association of SSBs intake with the hair loss condition in women.This online cross-sectional study,conducted from January 2022 to April 2022,recruited 1169 women aged 18-45 years from 31 provinces on mainland of China.We u sed the updated version of the 15-item Beverage Intake Questionnaire(BEVQ-15)to evaluate the usual beverage consumption over the past month and the Hair-Shedding Visual Scale for Asian Women(HSVS-A)to evaluate the extent of hair shedding.We utilized binary logistic regression to assess the association between SSBs intake and hair loss.Among 1169 female participants with an average age of 27.2,there were 211(18.0%)participants with hair loss.Compared with participants who never drank SSBs,those who drank m ore than 7 times/week or 1000 mL/week were more likely to have hair loss,with an adjusted odds ratio(OR)of 2.25(95%confidence interval(CI)=1.32,3.83)or 2.22(95%CI=1.28,3.85).Furthermore,the m ediation effect of anxiety status on the association between SSBs and female hair loss was observed,with a significant indirect effect(β_(indirect)=0.0199,95%CI=0.0032,0.0418).In conclusion,our study showed that w omen who consumed higher amounts of SSBs had an increased risk of hair loss,which might attract more attention from young women and benefit from reducing SSBs consumption.Further longitudinal and interventional studies are still needed to confirm the current association and provide evidenced-based health education.展开更多
Hair follicle stem cell(HFSC),capable of self-renewal and differentiation in hair follicle,represents an emerging stem cell model for regenerative medicine.The interaction between HFSC and dermal papilla cell(DPC)gove...Hair follicle stem cell(HFSC),capable of self-renewal and differentiation in hair follicle,represents an emerging stem cell model for regenerative medicine.The interaction between HFSC and dermal papilla cell(DPC)governs hair follicle development.FGF7 functions as a paracrine protein regulating epithelial proliferation,differentiation and migration.The single-cell transcriptome profling and immunofuorescence analysis demonstrated that FGF7 localizes at DPC,while FGF7 receptor(FGFR2)expresses in both DPC and HFSC.Through co-culture experiments of HFSC and DPC,the results indicated that FGF7 secreted from DPC promotes the proliferation of DPC and HFSC via Wnt signaling pathway and induces HFSC differentiation.Furthermore,CUT&Tag assay revealed genomic colocalization between FGF7 and pluripotency-related genes and GSK3β.Electrophoretic mobility shift assay(EMSA)demonstrated that FGF7 interacts with the promoter region of CISH and PRKX.This research provides valuable insights into the molecular mechanisms underlying the hair cycle.Understanding the interaction between HFSC and DPC,as well as the role of FGF7,may advance regenerative medicine and hair loss treatment.展开更多
Objective:To evaluate the effects of Stellera chamaejasme L.(S.chamaejasme,Rui Xiang Lang Du)extract on hair growth in a mouse model.Methods:The extract was prepared using 95%ethanol and topically applied as a 1%or 3%...Objective:To evaluate the effects of Stellera chamaejasme L.(S.chamaejasme,Rui Xiang Lang Du)extract on hair growth in a mouse model.Methods:The extract was prepared using 95%ethanol and topically applied as a 1%or 3%solution to the dorsal skin of shaved mice for 16 consecutive days.A control mouse group received an equal volume of vehicle for the same period.After 16 days,the dorsal skin was histologically examined through hematoxylin-eosin staining.Further,quantitative real time-polymerase chain reaction was performed on skin tissue lysates to evaluate the expression levels of mRNAs encoding proteins involved in hair growth,including WNT10A,noggin(NOG),transforming growth factor-β receptor 1(TBR1),epidermal growth factor(EGF),versican,fibroblast growth factor 10(FGF10),lymphoid enhancer-binding factor 1(LEF1),and transforming growth factor-β(TGF-β).Results:Compared with vehicle,S.chamaejasme extract dose-dependently enhanced hair growth.Histological analysis revealed that S.chamaejasme extract increased the number and diameter of hair follicles in subcutaneous tissue,as well as dermal layer thickness,which are indicative of anagen phase induction.Additionally,S.chamaejasme extract upregulated the mRNA expression levels of WNT10A,NOG,TBR1,EFG,FGF10,LEF1,and TGF-β.Conclusion:The results suggest that S.chamaejasme extract could be a potential treatment for promoting hair growth.展开更多
Skins expose to kinds of risk factors for damage,such as the hormone drugs,skin care products and ultraviolet radiation,which is accompanied by the production of excessive reactive oxygen species(ROS)and eventually le...Skins expose to kinds of risk factors for damage,such as the hormone drugs,skin care products and ultraviolet radiation,which is accompanied by the production of excessive reactive oxygen species(ROS)and eventually leads to hypertrichosis.This skin disease is not aesthetically pleasing and even causes psychological and spiritual problems such as inferiority,anxiety and irritability.Current therapies are limited and often unsatisfactory,such as pharmacological and physical therapies,which have adverse effects and cause the irreversible destruction of hair follicles.Gold nanoclusters have good biocompatibility and their biosynthesis in vivo is responsive to oxidative stress microenvironment(OSM),which could be a safe and effective drug for ROS-induced skin injury.In our study,we demonstrated that zero valence fluorescent gold nanoclusters(FGNCs)were in situ biosynthesized in the plucking-induced damaged skin but not in the normal skin after the administration of gold precursors(+3),while FGNCs inhibited hair follicle regeneration by negatively regulating nuclear transcription factor kappa B(NFκB)-mediated inflammatory response signaling pathway(NFκB/tumor necrosis factor-α(TNF-α)axis).This OSM-responsive in situ biosynthesis method is facile and safe and holds great promise for curing hypertrichosis associated with skin dermatitis and injury.展开更多
BACKGROUND Mesenchymal stem cells(MSCs)and their secretome have significant potential in promoting hair follicle development.However,the effects of MSC therapy have been reported to vary due to their heterogeneous cha...BACKGROUND Mesenchymal stem cells(MSCs)and their secretome have significant potential in promoting hair follicle development.However,the effects of MSC therapy have been reported to vary due to their heterogeneous characteristics.Different sources of MSCs or culture systems may cause heterogeneity of exosomes.AIM To define the potential of human adipose-derived MSC exosomes(hADSC-Exos)and human umbilical cord-derived MSC exosomes(hUCMSC-Exos)for improving dermal papillary cell proliferation in androgenetic alopecia.METHODS We conducted liquid chromatography-mass spectrometry proteomic analysis of hADSC-Exos and hUCMSC-Exos.Liquid chromatography-mass spectrometry suggested that hADSC-Exos were related to metabolism and immunity.Additionally,the hADSC-Exo proteins regulated the cell cycle and other 9 functional groups.RESULTS We verified that hADSC-Exos inhibited glycogen synthase kinase-3βexpression by activating the Wnt/β-catenin signaling pathway via cell division cycle protein 42,and enhanced dermal papillary cell proliferation and migration.Excess dihydrotestosterone caused androgenetic alopecia by shortening the hair follicle growth phase,but hADSC-Exos reversed these effects.CONCLUSION This study indicated that hair development is influenced by hADSC-Exo-mediated cell-to-cell communication via the Wnt/β-catenin pathway.展开更多
Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration vi...Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration via paracrine signaling;however,their clinical applications are limited by potential risks such as tumorigenesis and xenogeneic immune rejection,which are similar to the risks associated with other stem cell transplantations.The present study therefore focuses on small extracellular vesicles derived from hair follicle neural crest stem cells,which preserve the bioactive properties of the parent cells while avoiding the transplantation-associated risks.In vitro,small extracellular vesicles derived from hair follicle neural crest stem cells significantly enhanced the proliferation,migration,tube formation,and barrier function of perineurial cells,and subsequently upregulated the expression of tight junction proteins.Furthermore,in a rat model of sciatic nerve defects bridged with silicon tubes,treatment with small extracellular vesicles derived from hair follicle neural crest stem cells resulted in higher tight junction protein expression in perineurial cells,thus facilitating neural tissue regeneration.At 10 weeks post-surgery,rats treated with small extracellular vesicles derived from hair follicle neural crest stem cells exhibited improved nerve function recovery and reduced muscle atrophy.Transcriptomic and micro RNA analyses revealed that small extracellular vesicles derived from hair follicle neural crest stem cells deliver mi R-21-5p,which inhibits mothers against decapentaplegic homolog 7 expression,thereby activating the transforming growth factor-β/mothers against decapentaplegic homolog signaling pathway and upregulating hyaluronan synthase 2 expression,and further enhancing tight junction protein expression.Together,our findings indicate that small extracellular vesicles derived from hair follicle neural crest stem cells promote the proliferation,migration,and tight junction protein formation of perineurial cells.These results provide new insights into peripheral nerve regeneration from the perspective of perineurial cells,and present a novel approach for the clinical treatment of peripheral nerve defects.展开更多
Hair follicle stem cells(HFSCs)in the bulge are a multipotent adult stem cell population.They can periodically give rise to new HFs and even regenerate the epidermis and sebaceous glands during wound healing.An increa...Hair follicle stem cells(HFSCs)in the bulge are a multipotent adult stem cell population.They can periodically give rise to new HFs and even regenerate the epidermis and sebaceous glands during wound healing.An increasing number of biomarkers have been used to isolate,label,and trace HFSCs in recent years.Considering more detailed data from single-cell transcriptomics technology,we mainly focus on the important HFSC molecular markers and their regulatory roles in this review.展开更多
To explore the efficacy evaluation method of depilatory cream.60 subjects were selected as subjects,and statistical analysis was carried out on the depilation effect of large samples.The hair was removed with a razor ...To explore the efficacy evaluation method of depilatory cream.60 subjects were selected as subjects,and statistical analysis was carried out on the depilation effect of large samples.The hair was removed with a razor in the control area and with depilation cream in the sample area,Antera 3D skin imaging analyzer was used to image the experimental area before and after the experiment,and the hair growth was dynamically monitored at 7,14,21 and 28 days,measure the length of hair with instrument and measure the number of people.The hair length change rate and hair inhibition rate were dynamically monitored in 28 days.The hair length change rate was-10.5%and hair inhibition rate was 93.3%after 28 days,more intuitive display of the product depilation effect,so as to determine the efficacy of depilation products evaluation method.展开更多
Pubic hair removal has increased in frequency over recent years, with some degree of pubic hair grooming considered desirable by both men and women. Despite the increasing use of chemical depilatory creams for the rem...Pubic hair removal has increased in frequency over recent years, with some degree of pubic hair grooming considered desirable by both men and women. Despite the increasing use of chemical depilatory creams for the removal of hair in intimate regions, there is a lack of published data regarding the safety and efficacy of such products when used for full hair removal in the genital area. This study investigated the in-use cutaneous compatibility of two thioglycolate chemical depilatory creams for full male and female genital depilation through three single application studies. Initially, a preliminary study tested a standard male intimate chemical depilatory cream (Product A) on 18 male participants to ensure acceptable tolerance of the product. Following this, Study 1 applied Product A to 33 male participants whilst a standard female intimate chemical depilatory cream (Product B) was applied to 31 female participants in Study 2. Visual dermal assessments, together with participant-perceived assessment of tolerance, were conducted before application, and at 30 minutes, 24 and 72 hours after product removal. Study 1 reported 5 incidences (out of 33) of dermal irritation at 30 minutes post product removal, with 3 incidences remaining after 72 hours post product removal. Study 2 reported 4 incidences (out of 31) of dermal irritation at 30 minutes post product removal with 1 incidence remaining after 72 hours post product removal. Both chemical depilatory creams were concluded by a dermatologist, on the basis of dermal irritation and participant-perceived tolerance, to have achieved acceptable dermal tolerance when applied to the intended area of use for the maximum recommended application time. This data provides confidence in the safety of chemical depilatory creams when used for full removal of the pubic hair of men and women and adds to the existing published database, validating the safety and efficacy of these products for genital hair removal.展开更多
Hearing loss has become increasingly prevalent and causes considerable disability,thus gravely burdening the global economy.Irreversible loss of hair cells is a main cause of sensorineural hearing loss,and currently,t...Hearing loss has become increasingly prevalent and causes considerable disability,thus gravely burdening the global economy.Irreversible loss of hair cells is a main cause of sensorineural hearing loss,and currently,the only relatively effective clinical treatments are limited to digital hearing equipment like cochlear implants and hearing aids,but these are of limited benefit in patients.It is therefore urgent to understand the mechanisms of damage repair in order to develop new neuroprotective strategies.At present,how to promote the regeneration of functional hair cells is a key scientific question in the field of hearing research.Multi-ple signaling pathways and transcriptional factors trigger the activation of hair cell progenitors and ensure the maturation of newborn hair cells,and in this article,we first review the principal mechanisms underlying hair cell reproduction.We then further discuss therapeutic strategies involving the co-regulation of multiple signaling pathways in order to induce effective functional hair cell regeneration after degeneration,and we summarize current achievements in hair cell regeneration.Lastly,we discuss potential future approaches,such as small molecule drugs and gene therapy,which might be applied for regenerating functional hair cells in the clinic.展开更多
文摘BACKGROUND Exosome-based therapies represent a promising approach for hair regeneration.Unlike conventional treatments such as minoxidil and finasteride,exosomes deliver bioactive cargo that can stimulate dermal papilla cells,enhance angiogenesis,and modulate inflammatory pathways.However,variability in exosome sources,isolation techniques,and dosing protocols limits their clinical translation.AIM To synthesize findings from in vitro,preclinical and clinical studies,and to evaluate the efficacy,mechanisms,and challenges associated with exosome-based hair restoration therapies.METHODS A literature search was conducted using multiple databases(PubMed/Medline,Embase,Scopus,and Web of Science)employing terms for exosomes and hair regeneration for articles published in English to February 2025,following Preferred Reporting Items for Systematic Reviews and Meta-analyses guidelines.RESULTS A total of 27 studies(three in vitro,three pre-clinical,18 with both in vitro and preclinical component and three clinical)met the pre-defined search and inclusion criteria and were included in this review.CONCLUSION Exosome-based therapies hold immense promise for hair regeneration by leveraging their ability to modulate key signaling pathways and enhance hair follicle regeneration.While in vitro and preclinical studies demonstrate consistent efficacy across diverse exosome sources,methodological heterogeneity and a limited number of clinical studies warrant further clinical research to realize their full clinical potential for hair regeneration.
文摘A hair tonic containing 1.0%Arctium lappa root extract(ALRE)was evaluated for its efficacy and tolerability in Chinese consumers.ALRE was selected based on its ability to promote Collagen Type XVII Alpha 1(COL17A1)synthesis,along with other active ingredients targeting scalp health and follicular regeneration.In vitro assays confirmed that ALRE significantly enhanced COL17A1 expression.A 28-day clinical trial involving Chinese participants demonstrated that the tonic reduced hair loss by 37.61%and increased local hair density by 26.63%,with no reported adverse effects.These findings validate the product’s effectiveness in a distinct consumer population and highlight the importance of integrating mechanistic insights with clinical validation.Further research should explore long-term efficacy and demographic-specific responses to optimize its application.
文摘The anti-hair loss mechanism of Aquilaria sinensis leaf extract(ASE)has been studied by using metabolomics and network pharmacology.Metabolomics was utilized to comprehensively identify the active constituents of ASE,and the network pharmacology was used to elucidate their anti-hair loss mechanism,which was verified by molecular docking technology.572 active compounds were identified from the ASE by metabolomics methods,where there are 1447 corresponding targets and 492 targets related to hair loss,totaling 88 targets.20 core active substances were identified by constructing a network between common targets and active substances,which include vanillic acid,chorionic acid,caffeic acid and apigenin.The five key targets of TNF,TP53,IL6,PPARG,and EGFR were screened out by the PPI network analysis on 88 common targets.The GO and KEGG pathway enrichment analysis showed that the inflammation,hormone balance,cell growth,proliferation,apoptosis,and oxidative stress are involved.Molecular docking studies have confirmed the high binding affinity between core active compounds and key targets.The drug similarity assessment on these core compounds suggested that they have the potential to be used as potential hair loss treatment drugs.This study elucidates the complex molecular mechanism of ASE in treating hair loss,and provides a reference for the future applications in hair care products.
基金supported by grants from The National Natural Science Foundation of China(31772690)the Natural Science Foundation of Shanxi Province(201701D121106)PhD Research Startup Foundation of Changzhi Medical College(BS202308)。
文摘Objective This study aimed to comprehensively investigate the potential protective effects and underlying mechanisms of taurine against dihydrotestosterone(DHT)-induced androgenetic alopecia(AGA)in male C57BL/6 mice,with a focus on hair follicle cycle modulation,cellular proliferation/apoptosis,and key related signaling pathways.Methods Six-week-old female C57BL/6 mice were initially used to assess the hair growth-promoting potential of taurine.After acclimatization,they were randomly assigned to three groups(n=8):control(regular drinking water),taurine(drinking water containing 1%taurine),and minoxidil(topical 2%minoxidil,positive control).For the AGA study,male C57BL/6 mice were randomly divided into five groups(n=8):control(physiological saline),DHT(model group,1 mg/d DHT),DHT+low-dose taurine(1 mg/d DHT+2 mg/d taurine),DHT+high-dose taurine(1 mg/d DHT+10 mg/d taurine),and DHT+minoxidil(positive control,1 mg/d DHT+topical 2%minoxidil).One day before treatment initiation,dorsal hair was shaved with scissors,and residual hair was removed using a depilatory cream.DHT and taurine were administered via daily intraperitoneal injection.Hair regrowth was assessed by photographing the depilated area at regular intervals and quantified using a four-point grading system(0-3).Dorsal skin samples were collected on day 14 for histological analysis(H&E staining),immunofluorescence staining(Ki67 for proliferation,TUNEL for apoptosis),ELISA(DHT quantification),RT-qPCR,and Western blot analysis to evaluate the expression of key genes and proteins(androgen receptor(AR),transforming growth factor(TGF)‑β1,TGF‑β2,Dickkopf-1(DKK1)).Results In female mice,taurine supplementation significantly accelerated hair growth,with effects comparable to minoxidil.This was evidenced by an earlier transition from pink(telogen)to black(anagen)skin and increased hair growth scores.Histological analysis showed that taurine increased hair follicle count and dermal thickness.Immunofluorescence confirmed enhanced keratinocyte proliferation in the hair matrix.In the DHTinduced AGA model,DHT significantly extended the telogen phase,inhibited hair growth,increased skin DHT content,and induced hair follicle miniaturization.Taurine treatment,particularly at the high dose,effectively counteracted these effects:it promoted the telogen-to-anagen transition and improved hair growth scores.Histomorphometric analysis showed that taurine significantly restored DHT-induced reductions in dermal thickness,hair follicle count,hair bulb depth,and follicle size.Taurine treatment also reduced apoptosis and promoted the proliferation of hair follicle cells,as demonstrated by Ki67 and TUNEL assays.Crucially,RT-qPCR and Western blot analyses revealed that DHT significantly up-regulated the expression of AR,TGF‑β1,TGF‑β2,and DKK1 at both mRNA and protein levels in dorsal skin.Taurine administration markedly down-regulated the expression of these pathogenic factors,bringing them closer to the levels observed in the control group.Conclusion Taurine demonstrates significant efficacy in alleviating DHT-induced AGA in male C57BL/6 mice.Its protective effects are mediated through multi-faceted mechanisms.(1)Promoting hair follicle cycle progression:it accelerates the transition from telogen to anagen,counteracting DHT-induced prolongation of the telogen phase.(2)Modulating cellular dynamics:it stimulates the proliferation of hair matrix keratinocytes and reduces DHT-induced apoptosis within hair follicle cells.(3)Suppressing androgen-driven pathogenic pathways:it downregulates the expression of critical molecules in the AGA pathway,including AR,the cytokines TGF-β1 and TGF-β2,and the Wnt pathway inhibitor DKK1.Given its favorable safety profile and multi-targeted action,taurine emerges as a promising novel therapeutic candidate or adjunct for treating AGA.Further investigation into its clinical potential and precise molecular mechanisms is warranted.This study provides a robust preclinical foundation for considering taurine supplementation or topical application in hair loss management strategies.
基金financially supported by the National Natural Science Foundation of China(No.52225311)the Fundamental Research Funds for Central Universities.
文摘Hair coloring has emerged as an integral part of the cosmetic industry,particularly in response to the increasing global aging phenomenon.The natural melanin analog,polydopamine(PDA),has garnered considerable attention as an eco-friendly hair dye,and several kinds of polymerization ways of dopamine(DA)have been proposed including alkali catalysis,metal ion catalysis,strong oxidants,and enzyme-mediated oxidation reactions and polymerizations.Yet the controllability of polymerization and potential toxicity of involved metal ions are still in question.Inspired by the photoprotective mechanism in human skin,we have developed the melanin-inspired hair dyeing strategy that allowed for the in situ oxidative polymerization of DA under ultraviolet(UV)light.This polymerization was triggered by photobase generators(PBGs),a class of compounds that produced organic bases upon UV and sunlight irradiation.The resulting hair showed an adjustable color from light brown to black by tuning the ratio of DA and PBG(DA@PBG),the concentration of DA,and light exposure time.The dyed hairs showed excellent washing resistance and superior anti-static properties.Furthermore,Hair Color Spray DA@PBG also demonstrated a desirable hair dyeing effect and excellent biosecurity by simply spraying it on the hair under sunlight.This novel sunlight-induced method provided a new direction towards the preparation of natural hair dyes and could promote the development of green and safe hair dyes in colorful and brilliant artistic-grade hair coloring.
基金supported by the National Key Research and Development Program of China(2022YFD1300202)Collection,Utilization,and Innovation of Animal Resources by Research Institutes and Enterprises of Chongqing(Cqnyncw-kqlhtxm),Chongqing Modern Agricultural Industry Technology System(CQMAITS202413)National Training Program of Innovation and Entrepreneurship for Undergraduates(S202310635040)。
文摘The genetic regulation of hair density in animals remains poorly understood.The Dazu black goat,characterized by its black coarse hair and white skin,provides a unique model for dissecting coarse hair density(CHD).Using high-resolution micro-camera imaging,this study analyzed 905 skin images,33 skin transcriptomes,272 whole-genome sequences,and 182 downloaded transcriptomes.Morphological assessment from juvenile to adult stages revealed the thickening of hair shafts accompanied by a progressive decline in density,largely attributable to rapid surface expansion of the trunk skin.Transcriptomic comparison between high-and low-CHD individuals identified 572 differentially expressed genes(DEGs).A genome-wide association study detected 25 significant single nucleotide polymorphisms(P<9.07e-8)and mapped 48 annotated genes,with the most prominent association signal located near GJA1 on chr9.15931585-18621011.Literature review and Venn analysis highlighted six genes(GJA1,GPRC5D,CD1D,CD207,TFAM,and CXCL12)with documented roles in skin and hair biology,and three genes(GJA1,GPRC5D,and ATP6V1B1)overlapped with DEGs.Multiple-tissue transcriptomic profiling,western blotting,immunohistochemical staining,and skin single-cell RNA sequencing confirmed that GJA1 and GPRC5D were highly and specifically expressed in skin,particularly within hair follicles.Expression was localized predominantly to follicular stem cells and dermal papilla cells,suggesting a significant role in folliculogenesis and structural maintenance.Cross-validation using four public datasets further demonstrated positive correlations between GJA1 and GPRC5D expression and hair follicle density.The innovative micro-camera application allowed the elucidation of spatiotemporal patterns and genes associated with CHD,thereby addressing a significant knowledge gap in animal hair density.
基金supported by grants from the National Natural Science Foundation of China(82222017,82271183,and 81970883)the Key Research and Development Program of Hubei Province(2022BCA046)funding from the Fundamental Research Funds for the Central Universities(2042022kf0059).
文摘Although cisplatin is a widely used chemotherapeutic agent,it is severely toxic and causes irreversible hearing loss,restricting its application in clinical settings.This study aimed to determine the molecular mechanism underlying cisplatin-induced ototoxicity.Here,we established in vitro and in vivo ototoxicity models of cisplatininduced hair cell loss,and our results showed that reducing STING levels decreased inflammatory factor expression and hair cell death.In addition,we found that cisplatin-induced mitochondrial dysfunction was accompanied by cytosolic DNA,which may act as a critical linker between the cyclic GMP-AMP synthesis−stimulator of interferon genes(cGASSTING)pathway and the pathogenesis of cisplatin-induced hearing loss.H-151,a specific inhibitor of STING,reduced hair cell damage and ameliorated the hearing loss caused by cisplatin in vivo.This study underscores the role of cGASSTING in cisplatin ototoxicity and presents H-151 as a promising therapeutic for hearing loss.
基金funded by the National Natural Science Foundation of China Regional Innovation and Development Joint Fund(Sichuan)(No.U21A20417)the National Natural Science Foundation of China(No.31930067)。
文摘The androgenetic alopecia(AGA)is the most prevalent clinical manifestation of hair loss,believed to be associated with excessive dihydrotestosterone(DHT)caused by typeⅡ5α-reductase(5αR2).The utilization of oral finasteride(FNS),which selectively inhibits 5αR2,is frequently constrained by its adverse effects.Topical FNS formulations can mitigate adverse effects but often exhibit limited dermal permeability.Nanocarriers show great potential in augmenting the cutaneous permeation of loaded FNS due to their inherent properties of selective accumulation within the hair follicles(HFs).In this study,hollow mesoporous silica nanoparticles(HMSN)with varying sizes were utilized as the nanocarriers for FNS,following mixing with the Carbopol hydrogel(F@H/Gel)for direct topical application.Specifically,the influence of size on the targeted delivery of FNS to HFs,and its enhanced therapeutic efficacy for the AGA mice model was evaluated.Results showed that the HMSN,with a diameter of approximately 300 nm,exhibited significant enhancement in FNS retention within skin and HFs,as well as remarkably accelerated hair regrowth on an AGA mouse model.In conclusion,this FNS topical formulation has proved to be a viable approach in offering a secure and efficient treatment modality for AGA.
文摘Objective:To evaluate nurses’levels of knowledge and practice regarding preoperative hair removal and prevention of surgical site infection(SSI).Methods:The descriptive and cross-sectional research was conducted between February 2023 and May 2023 in a public hospital in northern Turkey with 123 nurses who agreed to participate in the study.Results:The results showed that 64.2%of the nurses thought that preoperative hair removal should be done by shaving with a razor,74%thought that hair removal should be performed the night before the operation,and 70.7%thought that the patient/patient’s relative should perform preoperative hair removal.Conclusions:This study found that nurses’levels of knowledge and practice regarding preoperative hair removal and prevention of SSI were not at the desired level.Following current resources and improving their levels of knowledge and practices are considered to be important for nurses.
基金supported by grants from the National Natural Science Foundation of China(82192862,82371157,82201303,and 82271173)the Natural Science Foundation of Jiangsu Province(BE2023653,BK20230025,and BK20200133)+1 种基金the China Postdoctoral Science Foundation(2020M681555)a Distinguished Young Scholar supported by the Medical Science and Technology Development Foundation,Nanjing Department of Health(JQX20003).
文摘Noise-induced hearing loss is a worldwide public health issue that is characterized by temporary or permanent changes in hearing sensitivity.This condition is closely linked to inflammatory responses,and interventions targeting the inflammatory gene tumor necrosis factoralpha(TNFα)are known to mitigate cochlear noise damage.TNFα-induced proteins(TNFAIPs)are a family of translucent acidic proteins,and TNFAIP6 has a notable association with inflammatory responses.To date,there have been few reports on TNFAIP6 levels in the inner ear.To elucidate the precise mechanism,we generated transgenic mouse models with conditional knockout of Tnfaip6(Tnfaip6 cKO).Evaluation of hair cell morphology and function revealed no significant differences in hair cell numbers or ribbon synapses between Tnfaip6 cKO and wild-type mice.Moreover,there were no notable variations in hair cell numbers or hearing function in noisy environments.Our results indicate that Tnfaip6 does not have a substantial impact on the auditory system.
文摘The association between sugar-sweetened beverages(SSBs)intake and hair loss condition might give scientific support for better dietary practices to prevent hair loss.We aimed to explore the association of SSBs intake with the hair loss condition in women.This online cross-sectional study,conducted from January 2022 to April 2022,recruited 1169 women aged 18-45 years from 31 provinces on mainland of China.We u sed the updated version of the 15-item Beverage Intake Questionnaire(BEVQ-15)to evaluate the usual beverage consumption over the past month and the Hair-Shedding Visual Scale for Asian Women(HSVS-A)to evaluate the extent of hair shedding.We utilized binary logistic regression to assess the association between SSBs intake and hair loss.Among 1169 female participants with an average age of 27.2,there were 211(18.0%)participants with hair loss.Compared with participants who never drank SSBs,those who drank m ore than 7 times/week or 1000 mL/week were more likely to have hair loss,with an adjusted odds ratio(OR)of 2.25(95%confidence interval(CI)=1.32,3.83)or 2.22(95%CI=1.28,3.85).Furthermore,the m ediation effect of anxiety status on the association between SSBs and female hair loss was observed,with a significant indirect effect(β_(indirect)=0.0199,95%CI=0.0032,0.0418).In conclusion,our study showed that w omen who consumed higher amounts of SSBs had an increased risk of hair loss,which might attract more attention from young women and benefit from reducing SSBs consumption.Further longitudinal and interventional studies are still needed to confirm the current association and provide evidenced-based health education.
基金supported by the National Key Research And Development Program of China(2022YFD1300204)。
文摘Hair follicle stem cell(HFSC),capable of self-renewal and differentiation in hair follicle,represents an emerging stem cell model for regenerative medicine.The interaction between HFSC and dermal papilla cell(DPC)governs hair follicle development.FGF7 functions as a paracrine protein regulating epithelial proliferation,differentiation and migration.The single-cell transcriptome profling and immunofuorescence analysis demonstrated that FGF7 localizes at DPC,while FGF7 receptor(FGFR2)expresses in both DPC and HFSC.Through co-culture experiments of HFSC and DPC,the results indicated that FGF7 secreted from DPC promotes the proliferation of DPC and HFSC via Wnt signaling pathway and induces HFSC differentiation.Furthermore,CUT&Tag assay revealed genomic colocalization between FGF7 and pluripotency-related genes and GSK3β.Electrophoretic mobility shift assay(EMSA)demonstrated that FGF7 interacts with the promoter region of CISH and PRKX.This research provides valuable insights into the molecular mechanisms underlying the hair cycle.Understanding the interaction between HFSC and DPC,as well as the role of FGF7,may advance regenerative medicine and hair loss treatment.
基金supported by an intramural research grant(Development of Data Utilization Technology for Natural Product Research,2E33521)from the Korea Institute of Science and Technology(KIST)the data were deposited in the KIST Dashboard.
文摘Objective:To evaluate the effects of Stellera chamaejasme L.(S.chamaejasme,Rui Xiang Lang Du)extract on hair growth in a mouse model.Methods:The extract was prepared using 95%ethanol and topically applied as a 1%or 3%solution to the dorsal skin of shaved mice for 16 consecutive days.A control mouse group received an equal volume of vehicle for the same period.After 16 days,the dorsal skin was histologically examined through hematoxylin-eosin staining.Further,quantitative real time-polymerase chain reaction was performed on skin tissue lysates to evaluate the expression levels of mRNAs encoding proteins involved in hair growth,including WNT10A,noggin(NOG),transforming growth factor-β receptor 1(TBR1),epidermal growth factor(EGF),versican,fibroblast growth factor 10(FGF10),lymphoid enhancer-binding factor 1(LEF1),and transforming growth factor-β(TGF-β).Results:Compared with vehicle,S.chamaejasme extract dose-dependently enhanced hair growth.Histological analysis revealed that S.chamaejasme extract increased the number and diameter of hair follicles in subcutaneous tissue,as well as dermal layer thickness,which are indicative of anagen phase induction.Additionally,S.chamaejasme extract upregulated the mRNA expression levels of WNT10A,NOG,TBR1,EFG,FGF10,LEF1,and TGF-β.Conclusion:The results suggest that S.chamaejasme extract could be a potential treatment for promoting hair growth.
基金supported by the National Natural Science Foundation of China(Nos.82061148012,82027806,82372220,21974019)the National Key Research and Development Program of China(No.2017YFA0205300)the Primary Research&Development Plan of Jiangsu Province(No.BE2019716)。
文摘Skins expose to kinds of risk factors for damage,such as the hormone drugs,skin care products and ultraviolet radiation,which is accompanied by the production of excessive reactive oxygen species(ROS)and eventually leads to hypertrichosis.This skin disease is not aesthetically pleasing and even causes psychological and spiritual problems such as inferiority,anxiety and irritability.Current therapies are limited and often unsatisfactory,such as pharmacological and physical therapies,which have adverse effects and cause the irreversible destruction of hair follicles.Gold nanoclusters have good biocompatibility and their biosynthesis in vivo is responsive to oxidative stress microenvironment(OSM),which could be a safe and effective drug for ROS-induced skin injury.In our study,we demonstrated that zero valence fluorescent gold nanoclusters(FGNCs)were in situ biosynthesized in the plucking-induced damaged skin but not in the normal skin after the administration of gold precursors(+3),while FGNCs inhibited hair follicle regeneration by negatively regulating nuclear transcription factor kappa B(NFκB)-mediated inflammatory response signaling pathway(NFκB/tumor necrosis factor-α(TNF-α)axis).This OSM-responsive in situ biosynthesis method is facile and safe and holds great promise for curing hypertrichosis associated with skin dermatitis and injury.
基金Supported by the Peak Disciplines(Type IV)of Institutions of Higher Learning in Shanghai and the China Postdoctoral Science Foundation,No.2022M722409.
文摘BACKGROUND Mesenchymal stem cells(MSCs)and their secretome have significant potential in promoting hair follicle development.However,the effects of MSC therapy have been reported to vary due to their heterogeneous characteristics.Different sources of MSCs or culture systems may cause heterogeneity of exosomes.AIM To define the potential of human adipose-derived MSC exosomes(hADSC-Exos)and human umbilical cord-derived MSC exosomes(hUCMSC-Exos)for improving dermal papillary cell proliferation in androgenetic alopecia.METHODS We conducted liquid chromatography-mass spectrometry proteomic analysis of hADSC-Exos and hUCMSC-Exos.Liquid chromatography-mass spectrometry suggested that hADSC-Exos were related to metabolism and immunity.Additionally,the hADSC-Exo proteins regulated the cell cycle and other 9 functional groups.RESULTS We verified that hADSC-Exos inhibited glycogen synthase kinase-3βexpression by activating the Wnt/β-catenin signaling pathway via cell division cycle protein 42,and enhanced dermal papillary cell proliferation and migration.Excess dihydrotestosterone caused androgenetic alopecia by shortening the hair follicle growth phase,but hADSC-Exos reversed these effects.CONCLUSION This study indicated that hair development is influenced by hADSC-Exo-mediated cell-to-cell communication via the Wnt/β-catenin pathway.
基金supported by the National Natural Science Foundation of China,No.81571211(to FL)the Natural Science Foundation of Shanghai,No.22ZR1476800(to CH)。
文摘Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration via paracrine signaling;however,their clinical applications are limited by potential risks such as tumorigenesis and xenogeneic immune rejection,which are similar to the risks associated with other stem cell transplantations.The present study therefore focuses on small extracellular vesicles derived from hair follicle neural crest stem cells,which preserve the bioactive properties of the parent cells while avoiding the transplantation-associated risks.In vitro,small extracellular vesicles derived from hair follicle neural crest stem cells significantly enhanced the proliferation,migration,tube formation,and barrier function of perineurial cells,and subsequently upregulated the expression of tight junction proteins.Furthermore,in a rat model of sciatic nerve defects bridged with silicon tubes,treatment with small extracellular vesicles derived from hair follicle neural crest stem cells resulted in higher tight junction protein expression in perineurial cells,thus facilitating neural tissue regeneration.At 10 weeks post-surgery,rats treated with small extracellular vesicles derived from hair follicle neural crest stem cells exhibited improved nerve function recovery and reduced muscle atrophy.Transcriptomic and micro RNA analyses revealed that small extracellular vesicles derived from hair follicle neural crest stem cells deliver mi R-21-5p,which inhibits mothers against decapentaplegic homolog 7 expression,thereby activating the transforming growth factor-β/mothers against decapentaplegic homolog signaling pathway and upregulating hyaluronan synthase 2 expression,and further enhancing tight junction protein expression.Together,our findings indicate that small extracellular vesicles derived from hair follicle neural crest stem cells promote the proliferation,migration,and tight junction protein formation of perineurial cells.These results provide new insights into peripheral nerve regeneration from the perspective of perineurial cells,and present a novel approach for the clinical treatment of peripheral nerve defects.
基金National Natural Science Foundation of China,No.82173446the Youth Training Program of the Army Medical University,No.2018XQN01.
文摘Hair follicle stem cells(HFSCs)in the bulge are a multipotent adult stem cell population.They can periodically give rise to new HFs and even regenerate the epidermis and sebaceous glands during wound healing.An increasing number of biomarkers have been used to isolate,label,and trace HFSCs in recent years.Considering more detailed data from single-cell transcriptomics technology,we mainly focus on the important HFSC molecular markers and their regulatory roles in this review.
文摘To explore the efficacy evaluation method of depilatory cream.60 subjects were selected as subjects,and statistical analysis was carried out on the depilation effect of large samples.The hair was removed with a razor in the control area and with depilation cream in the sample area,Antera 3D skin imaging analyzer was used to image the experimental area before and after the experiment,and the hair growth was dynamically monitored at 7,14,21 and 28 days,measure the length of hair with instrument and measure the number of people.The hair length change rate and hair inhibition rate were dynamically monitored in 28 days.The hair length change rate was-10.5%and hair inhibition rate was 93.3%after 28 days,more intuitive display of the product depilation effect,so as to determine the efficacy of depilation products evaluation method.
文摘Pubic hair removal has increased in frequency over recent years, with some degree of pubic hair grooming considered desirable by both men and women. Despite the increasing use of chemical depilatory creams for the removal of hair in intimate regions, there is a lack of published data regarding the safety and efficacy of such products when used for full hair removal in the genital area. This study investigated the in-use cutaneous compatibility of two thioglycolate chemical depilatory creams for full male and female genital depilation through three single application studies. Initially, a preliminary study tested a standard male intimate chemical depilatory cream (Product A) on 18 male participants to ensure acceptable tolerance of the product. Following this, Study 1 applied Product A to 33 male participants whilst a standard female intimate chemical depilatory cream (Product B) was applied to 31 female participants in Study 2. Visual dermal assessments, together with participant-perceived assessment of tolerance, were conducted before application, and at 30 minutes, 24 and 72 hours after product removal. Study 1 reported 5 incidences (out of 33) of dermal irritation at 30 minutes post product removal, with 3 incidences remaining after 72 hours post product removal. Study 2 reported 4 incidences (out of 31) of dermal irritation at 30 minutes post product removal with 1 incidence remaining after 72 hours post product removal. Both chemical depilatory creams were concluded by a dermatologist, on the basis of dermal irritation and participant-perceived tolerance, to have achieved acceptable dermal tolerance when applied to the intended area of use for the maximum recommended application time. This data provides confidence in the safety of chemical depilatory creams when used for full removal of the pubic hair of men and women and adds to the existing published database, validating the safety and efficacy of these products for genital hair removal.
基金supported by grants from the National Key R&D Program of China(2021YFA1101300,2021YFA1101800,2020YFA0112503)the National Natural Science Foundation of China(82030029,81970882,82000984,92149304)+4 种基金the Science and Technology Department of Sichuan Province(2021YFS0371)the Shenzhen Fundamental Research Program(JCYJ20190814093401920,JCYJ20210324125608022)the China National Postdoctoral Program for Innovative Talents(BX20200082)the China Postdoctoral Science Foundation(2020M681468)the Open Research Fund of State Key Laboratory of Genetic Engineering,Fudan University(SKLGE-2104).
文摘Hearing loss has become increasingly prevalent and causes considerable disability,thus gravely burdening the global economy.Irreversible loss of hair cells is a main cause of sensorineural hearing loss,and currently,the only relatively effective clinical treatments are limited to digital hearing equipment like cochlear implants and hearing aids,but these are of limited benefit in patients.It is therefore urgent to understand the mechanisms of damage repair in order to develop new neuroprotective strategies.At present,how to promote the regeneration of functional hair cells is a key scientific question in the field of hearing research.Multi-ple signaling pathways and transcriptional factors trigger the activation of hair cell progenitors and ensure the maturation of newborn hair cells,and in this article,we first review the principal mechanisms underlying hair cell reproduction.We then further discuss therapeutic strategies involving the co-regulation of multiple signaling pathways in order to induce effective functional hair cell regeneration after degeneration,and we summarize current achievements in hair cell regeneration.Lastly,we discuss potential future approaches,such as small molecule drugs and gene therapy,which might be applied for regenerating functional hair cells in the clinic.
