BACKGROUND Cervical cancer is a prevalent form of cancer affecting women worldwide and it is the second most common cancer among women in Indonesia,accounting for 8.5%of all cancer-related deaths.Cervical cancer progr...BACKGROUND Cervical cancer is a prevalent form of cancer affecting women worldwide and it is the second most common cancer among women in Indonesia,accounting for 8.5%of all cancer-related deaths.Cervical cancer progression can be evaluated through laboratory tests to detect anaemia,an increased platelet count,and elevated inflammatory markers,therefore,effective laboratory examination is crucial for early detection and treatment of cervical cancer.AIM To evaluate the association between laboratory findings(haematology,haematology index,and inflammatory index)and the clinical stage of cervical cancer.METHODS This cross-sectional study analyzed adult cervical cancer patients’data from medical records and laboratory results including sociodemographic status,histopathological finding,clinical stage,and complete haematology examination.Numerical data was analyzed by the one-way ANOVA(normal data distribution),while the Kruskal-Wallis test was used for non-parametric data(abnormal distribution),followed by appropriate post-hoc analysis.The categorical data was analyzed by the Chi-square or Fisher Exact tests.The significance level was established at a P value<0.05.RESULTS This study involved the data of 208 adult cervical cancer patients and found no association between age,marital history,parity history,hormonal contraceptive use and cervical cancer stages.There were significant differences in the clinical laboratory test results based on the clinical stage of cervical cancer,including haemoglobin levels(P<0.001),leucocytes(P<0.001),neutrophils(P<0.001),monocytes(P=0.002),lymphocytes(P=0.006),platelets(P<0.001),neutrophil-lymphocyte ratio/NLR(P<0.001),lymphocyte-monocyte ratio/LMR(P<0.001),and plateletlymphocyte ratio/PLR(P<0.001).There were also significant differences in the systemic inflammatory index(SII)and systematic inflammatory response index(SIRI)between stage III+IV cervical cancer and stage II(SII P<0.001;SIRI P=0.001)and stage I(SII P<0.001;SIRI P=0.016),associated with the shifts in previously mentioned complete haematological values with cancer advancement.CONCLUSION The haematological parameters,inflammatory haematological ratios,and inflammatory indices exhibited significant differences between cervical cancer stages,therefore these tests can be utilized to evaluate cervical cancer progression.展开更多
The reduced efficiency of antibiotic growth promoters in livestock, and the unavoidable residual effects on livestock products such as egg, meat and milk have pushed researchers to rush towards the use of phytobiotics...The reduced efficiency of antibiotic growth promoters in livestock, and the unavoidable residual effects on livestock products such as egg, meat and milk have pushed researchers to rush towards the use of phytobiotics in animal feed. This study was conducted at the Teaching and Research Farm of the University of Dschang to evaluate the Effect of <span style="white-space:nowrap;"><i>Cucurma longa</i></span> powder as feed additive on hemato-serological parameters in Guinea pigs. A total of 120 three weeks old Guinea pigs (60 males and 60 females), with an average weight of 150 ± 20 g were used. The animals were randomly distributed into four treatments in a completely randomized design with 30 animals per treatment (15 males and 15 females). Four experimental treatments were used by adding 0%, 0.25%, 0.5% and 1% <i>C. longa</i> in the diet corresponding to R0, R0.25%, R0.5% and R1% respectively. At the 8th week of age, 12 Guinea pigs (6 males and 6 females) per treatment were fasted for 12 hours and sacrificed by cervical dislocation. Blood was rapidly collected from the jugular vein for the evaluation of haematological and biochemical parameters. Results revealed that the inclusion of <span style="white-space:normal;"><i></span>Curcuma longa<span style="white-space:normal;"></i></span> at 0.25% significantly (p < 0.05) increased the concentration of hematocrit, lymphocytes, monocytes, granulocytes and platelets. A significant decrease (p < 0.05) was observed in hemoglobin, red blood cells, white blood cells, serum lipids, globulin, AST, ALT, and urea. It was concluded that the use of <span style="white-space:normal;"><i></span>Curcuma longa<span style="white-space:normal;"></i></span> powder at 0.25% as feed additive positively regulates blood serum parameters of Guinea pigs.展开更多
Background: Adequate selection of a prospective whole blood donor protects his health and safety of the recipient. Objectives: The main objective of this study was to determine the haematology parameters of apparently...Background: Adequate selection of a prospective whole blood donor protects his health and safety of the recipient. Objectives: The main objective of this study was to determine the haematology parameters of apparently healthy prospective whole blood donors. Participants and Methods: This was a hospital based prospective study carried out from August to October 2020 at the blood transfusion unit of the Lagos State University Teaching Hospital (LASUTH), Ikeja, Nigeria. A structured pretested questionnaire was used for data collection. The socio demographic status and the haematology parameters of apparently healthy prospective whole blood donors who tested negative for HIV, hepatitis B and C markers were captured. Obtained data were analysed with the statistical package for the social scientist software version 20. Results: One hundred male (97.1%) and three female (2.9%) apparently healthy prospective whole blood donors were studied. The median age of study subjects was 30 years. Obtained median haematology parameter values were 13 g/dl, 40%, 4.9/nl and 203.9/nl for haemoglobin concentration, haematocrit, total white cell and platelet counts respectively. The median values for the mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) of participants were 32.6 g/dl, 27.7 pg and 85.7 fl respectively. Observed prevalence of subnormal haematology parameters for haemoglobin concentration, total white cells, platelets were 12.6%, 25.2%, and 13.6% respectively. Also subnormal values for MCHC, MCH, MCV were 11.7%, 26.2%, and 16.5% respectively among prospective whole blood donors in this study. No higher than normal haematology parameter values were observed. Median values for erythrocyte sedimentation rate was 8.4 mm/hr. Conclusion: A significant percentage of apparently healthy prospective whole blood donors had subnormal haematology parameters values. Obtained normal values in our study are comparable with local reference range reports from previous studies in Nigeria and other parts of Africa. 124947 .展开更多
Basic haematological parameters were studied in adult, sexually mature individuals of colour morphs (striata and maculata) in the populations of Pelophylax ridibundus living in biotopes with various degrees of anthrop...Basic haematological parameters were studied in adult, sexually mature individuals of colour morphs (striata and maculata) in the populations of Pelophylax ridibundus living in biotopes with various degrees of anthropogenic pollution (control, domestic sewage pollution and heavy metal pollution) in Southern Bulgaria. We found that in the polluted biotopes in individuals of both morphs of P. ridibundus the blood parameters: erythrocyte (RBC) and leukocyte (WBS) count, haemoglobin concentration (Hb), haematocrit (PCV) were statistically reliably higher, the number of lymphocytes (Ly) decreased, and the parameters: mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC), mean cell volume (MCV) and differential blood formula changed considerably in comparison with the control group. In terms of anthropogenic pollution, the average values of RBC and Hb were higher in P. ridibundus of striata morph.展开更多
The present study investigated the effect of partial replacement of soybean meal(Glycine max) with canola meal(CM)(Brassica napus) on the growth performance,haematology,serum biochemistry and meat quality characterist...The present study investigated the effect of partial replacement of soybean meal(Glycine max) with canola meal(CM)(Brassica napus) on the growth performance,haematology,serum biochemistry and meat quality characteristics of female Japanese quails in a 35-day feeding trial.One hundred and forty6-week-old quails 158.28 ± 11.919 g were randomly allocated to 5 isonitrogenous and isoenergetic experimental diets: control diet(CM0; with no CM inclusion); CM0 with 2.5%(CM25),5.0%(CM50),12.5%(CM125) and 17.5%(CM175) soybean meal replaced with CM.Average weekly gain(AWG) and feed conversion efficiency(FCE) were determined.Haematology,serum biochemistry,carcass traits and meat quality parameters were determined at slaughter.Quails fed CM175 had the lowest(P < 0.05) feed intake whereas no differences were observed among the other 4 diets.No dietary effects on AWG,FCE and haematological parameters were observed.Serum biochemical parameters were not influenced by diets with the exception of alkaline phosphatase(ALP),where quails fed CM25 had higher ALP(161.0 U/L) than those fed CM0(37.25 U/L).Quails fed CM25 had the highest chroma(7.39) while those fed CM125 had the lowest(3.58) at 24 h post-slaughter.Diets had no influence(P > 0.05) on cooking losses and peak positive force of quail meat.It was concluded that CM can replace soybean in quail diets up to 12.5% without compromising growth performance,health and quality of meat.Inclusion levels beyond 12.5% promoted poor voluntary feed intake and thus may require feed additives to enhance utilization.展开更多
Objective:To investigate the haematology and biochemistry values of apparently healthy captive sand cats kept in Al Ain Wildlife Park and Resort,United Arab Emirates,with a view to establishing baseline values.Methods...Objective:To investigate the haematology and biochemistry values of apparently healthy captive sand cats kept in Al Ain Wildlife Park and Resort,United Arab Emirates,with a view to establishing baseline values.Methods:Blood was collected from the femoral vein using aseptic techniques,kept in a cool box and sent to laboratory for analysis.The blood was analysed for haematological and biochemical values using veterinary hematology and chemistry analysers(ABX ABC Vet,Horiba ABX SAS^(®)Montpellier,France).Results:Haematological values were within the normal ranges recorded in domestic cats and there was no statistical difference between values found in males and females.Aspartate aminotransferase values were higher(P<0.05)in males compared to female cats.Serum protein,creatinine and glucose values for females were significantly(P<0.05)higher than those for males.Creatinine kinase,urea and lactate dehydrogenase levels were higher than those reported for normal domestic cat,but there was no statistical difference(P>0.05)between males and females values.Conclusions:Our results present reference ranges for haematology and biochemistry parameters in captive sand cats.These values will be important for diagnosis of various diseases and monitoring of treatments.展开更多
We specifically discuss the mechanisms of the pathogenesis,diagnosis,and management of blastic plasmacytoid dendritic cell neoplasm(BPDCN),a rare but aggressive haematologic malignancy characterized by frequent skin m...We specifically discuss the mechanisms of the pathogenesis,diagnosis,and management of blastic plasmacytoid dendritic cell neoplasm(BPDCN),a rare but aggressive haematologic malignancy characterized by frequent skin manifestations and systemic dissemination.The article enriches our understanding of BPDCN through detailed case reports showing the clinical,immunophenotypic,and histopathological features that are critical for diagnosing this disease.These cases highlight the essential role of pathologists in employing advanced immunophenotyping techniques to accurately identify the disease early in its course and guide treatment decisions.Furthermore,we explore the implications of these findings for management strategies,emphasizing the use of targeted therapies such as tagraxofusp and the potential of allogeneic haematopoietic stem cell transplantation in achieving remission.The editorial underscores the importance of interdisciplinary approaches in managing BPDCN,pointing towards a future where precision medicine could significantly improve patient outcomes.展开更多
The study aimed to investigate the Haematological and Serum Biochemical indices of finisher broiler chickens fed graded levels of Euphorbia heterophylla leaf meal (EHLM) also known as spurge weed. The birds were allot...The study aimed to investigate the Haematological and Serum Biochemical indices of finisher broiler chickens fed graded levels of Euphorbia heterophylla leaf meal (EHLM) also known as spurge weed. The birds were allotted into six dietary treatments of ten birds segregated into three replicates each. The diets formulated with EHLM were included at 0%, 5%, 10%, 15%, 20% and 25% levels in diets 1, 2, 3, 4, 5 and 6 respectively to replace soybean. Each treatment was replicated three times in a completely randomized design. Uncoagulated blood samples were collected from the birds at the end of the 56 days feeding trial and analysed for packed cell volume (PCV), haemoglobin concentrate (Hb), red blood cells (RBC) and white blood cells (WBC). The mean corpuscular haemoglobin volume (MCV), mean corpuscular haemoglobin (MCH), platelets, neutrophils, lymphocytes, monocytes, eosinophils, and basophils were calculated using PCV, RBC and Hb. The blood meant for serological analysis was centrifuged at 1000 G for 10 minutes, after which the serum was separated and used for determining serum total protein (Tp), Albumin, Serum glutamic oxaloacetic transaminase (SGOT) and Serum glutamic pyruvic transaminase (SGPT). The results revealed that the control group had significantly higher values of PCV, RBC, and Hb compared to other treatment groups. However, the values of MCV, MCH, lymphocytes, heterophils, and eosinophils were similar to the control. The biochemical parameters showed significant differences among treatment groups, but not significantly different from the control. The study concluded that EHLM may not pose a health challenge to broiler chickens at levels of 5 - 15 percent, but improved health, immunity and performance can be achieved at the 15% inclusion level.展开更多
Due to its toxicity, dichlorvos—a common organophosphate pesticide—poses significant risks to human health. This study utilized male Wistar rats to explore the potential protective effects of L-arginine supplementat...Due to its toxicity, dichlorvos—a common organophosphate pesticide—poses significant risks to human health. This study utilized male Wistar rats to explore the potential protective effects of L-arginine supplementation against dichlorvos-induced toxicity, focusing on cardiotoxicity, haematotoxicity and oxidative stress. The rats were divided into four groups: Control, L-arginine (L), Dichlorvos (D), and L-arginine + Dichlorvos (L + D). Dichlorvos was administered to the D group, L-arginine (100 mg/kg) to the L group, and both L-arginine and dichlorvos to the L + D group. The study evaluated various parameters, including cardiovascular, oxidative stress markers, and haematological indices. Significant changes in haematological parameters such as haemoglobin (Hb), haematocrit (HCT), and red blood cell count (RBC) indicated haematotoxicity after dichlorvos administration. Additionally, elevated cardiac markers, including lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB), suggested cardiotoxic effects. Exposure to dichlorvos also resulted in decreased antioxidant enzyme levels and increased oxidative stress indicators like malondialdehyde (MDA). Remarkably, L-arginine supplementation mitigated the damage caused by dichlorvos. It normalized the altered haematological parameters, demonstrating its protective effect against haematotoxicity. The rise in cardiac markers was reduced with L-arginine supplementation, indicating protection against cardiotoxicity. Moreover, L-arginine significantly decreased oxidative stress, as evidenced by lower MDA levels and restored antioxidant enzyme activity. In conclusion, L-arginine supplementation in male Wistar rats showed promising protective effects against dichlorvos-induced cardiotoxicity, haematotoxicity and oxidative stress. This suggests that L-arginine may offer a beneficial intervention to mitigate the adverse effects of dichlorvos on blood and heart health, paving the way for potential treatments for pesticide poisoning.展开更多
Objective:To investigate the antidiabetic properties of aqueous extract of stem bark of Afzelia africana(A.africana)and its beneficial effect on haematological parameters in streptozotocin induced diabetic rats.Method...Objective:To investigate the antidiabetic properties of aqueous extract of stem bark of Afzelia africana(A.africana)and its beneficial effect on haematological parameters in streptozotocin induced diabetic rats.Methods:A total of 30 rats including 24 diabetic and 6 normal rats were used for this study.Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin.After being confirmed diabetic,animals were orally treated with distilled water or extracts at 100 or 200 mg/kg body weight daily for 10 days.The haematological parameters including red blood and white blood cells and their functional indices were evaluated in diabetic treated groups compared with the controls.Results:The extract significantly reduced the blood glucose levels while the best result was obtained at 200 mg/kg body weight The feed and water intake in diabetic rats were significantly reduced while weight loss was minimized at both dosages.Similarly,the levels of red blood,white blood cells and their functional indices were significantly improved after extract administration at both doses.Conclusions:It can be concluded that the aqueous extract of bark of A.africana possesses antihyperglycemic properties.In addition,the extract can prevent various complications of diabetes and improve some haematological parameters.Further experimental investigation is needed to exploit its relevant therapeutic effect to substantiate its ethnomedicinal usage.展开更多
Objective:To evaluate clinical and hematological aspects of dogs naturally infected with Hepatozoon canis(H.canis)presented at the Small Animal Clinics of Guru Angad Dev Veterinary and Animal Sciences University,Ludhi...Objective:To evaluate clinical and hematological aspects of dogs naturally infected with Hepatozoon canis(H.canis)presented at the Small Animal Clinics of Guru Angad Dev Veterinary and Animal Sciences University,Ludhiana.Methods:Blood films of 34 naturally infected dogs were examined for haematological alterations and parasitaemia.Signalment and clinical signs were recorded from the animals.Clinical histories were filled oul during the consultation.Results:Of the 34 positive dogs by Giemsa stained peripheral blood films.88.23%presented parasilaemia by H.canis only,while 11.77%had the combination of H.canis,Babesia sp.and Ehrlichia sp.Young male dogs less than oue-year-old,of nondescript breed,were the most commonly affected.And 26.47%were presented with anorexia/inappetence as the only clinical symptom.Other clinical symptoms were mild to moderate fever,pale mucosae and lethargy;a few were also showing the signs of vomiting and diarrhoea.Haematological alterations showed mainly normochromic-normocytic anaemia,leukocytosis and neutrophilia.Conclusions:The findings of this study substantiate that H.canis caused clinical and haematological alterations of the varied intensity in dogs,even with low parasilaemia.should he taken into consideration.展开更多
Objective:To explore cytotoxic activity of ethanol extract of Alpinia calcarata Rose(EEAC) rhizome against Ehiiich ascites carcinoma(EAC) tumor bearing Swiss Albino mice.Methods: In the present study,its anti-neoplast...Objective:To explore cytotoxic activity of ethanol extract of Alpinia calcarata Rose(EEAC) rhizome against Ehiiich ascites carcinoma(EAC) tumor bearing Swiss Albino mice.Methods: In the present study,its anti-neoplastic activity has been studied by monitoring parameters like tumor weight measurement,survival time,tumor cell growth inhibition,haematological characteristics etc.Results:It was found that EEAC at dose 8 mg/kg/day(i.p.) significantly decreased tumor weight(62.0%;P【0.01),increased life span(70.25%;P 【0.01) and reduced tumor cell growth rate(85.7%;P【0.01) in comparison to those of EAC bearing mice.The plant extract also improved the depleted haematological parameters like RBC WBC,Hb%,differential counts(e.g.lymphocytes,neutrophils,monocytes etc) of EAC bearing mice towards normal.The host toxic effects were not very high and recovered gradually towards normal within a few days after treatment.ConchiskHis:EEAC exhibits potent in vivo cytotoxic activity against EAC tumor bearing Swiss Albino mice.So,the plant can be considered as a probable new source of antitumor agents.展开更多
To investigate the effects ofMoringa oleifera leaf meal （MOLM） on the performance and haematology of broiler finisher using 120 Cobb chicks of 4 weeks old broiler chicks. The birds were assigned randomly into four d...To investigate the effects ofMoringa oleifera leaf meal （MOLM） on the performance and haematology of broiler finisher using 120 Cobb chicks of 4 weeks old broiler chicks. The birds were assigned randomly into four dietary treatments containing MOLM at 0, 6%, 8%, and 10% （treatments 1, 2, 3 and 4） inclusion levels, respectively, in a complete randomized design experiment. The effect of the dietary treatments on the growth performance and blood parameters of the broiler finisher was determined. MOLM at a rate of 6% （T2）, 8% （T3）, and 10% （T4） of the diets （as fed basis） to replace 3.2%, 5.6% and 8.6% of the crude protein （CP） of the control diet. The daily feed, dry matter and CP intake of the chicks fed MOLM diets were higher （p〈0.05） than those fed the control diet. Average weight gain （AWG） of birds fed MOLM diets reduced as inclusion levels increases 09〈0.05） than those fed the control diet. Chicks fed T2 showed higher （p〈0.05） AWG than those on T3 and T4. Feed conversion ratios （FCR, weight gain/g and feed intake） were higher for chicks fed MOLM. MOLM showed minimal deleterious effects in birds. However, birds fed T2 （6%） diets recorded significantly （p〈0.05） the highest body weight gain. There were significant differences （p〈0.05） among groups in packed cell volume （PVC） of birds. The haemoglobin （Hb）, mean corpuscular volume （MCV）, mean corpuscular haemoglobin （MCH） and mean corpuscular haemoglobin concentration （MCHC） counts showed no significant difference （p〉0.05） among treatments. The results indicated that at 10% in the diets of broiler finisher chick, MOLM could be substituted with expensive conventional protein sources without any deleterious effects on performance and blood parameters of broiler finisher chicken.展开更多
Bt corn “MON810: Ajeeb YG” produces delta endotoxins in the whole plant due to the genetic modification process. The chemical analysis of this variety showed significant differences from its conventional counterpart...Bt corn “MON810: Ajeeb YG” produces delta endotoxins in the whole plant due to the genetic modification process. The chemical analysis of this variety showed significant differences from its conventional counterpart “Ajeeb”. Further, feeding studies on rats were designed to complete assessing the safety of “MON810: Ajeeb YG”. Three groups of rats (6 males and 6 females’ rats/group) were fed on control diet, non-Bt corn and Bt corn for 1.5 months and 3 months. After 1.5 months, 3 males and 3 females from each group were sacrificed, and after another 1.5 months, 3 males and 3 females from each group and their offspring were sacrificed. Histopathological examination, blood haematology and serum biochemical analysis were determined. Results indicated significant differences among the tested parameters in the three groups especially Bt group. Severe changes in the liver of Bt group after 3 months were observed.展开更多
Background: Measurement of haematological parameters has been historically helpful in the diagnosis of many diseases in endurance sportsmen. The modifications of these parameters during endurance race have not yet bee...Background: Measurement of haematological parameters has been historically helpful in the diagnosis of many diseases in endurance sportsmen. The modifications of these parameters during endurance race have not yet been evaluated in many African countries. Objectives: To determine haematological values before and immediately after a half-marathon event, as well as within 24 hours after the race and to analyze the changes observed. Methods: A cross-sectional study was conducted from 10 to 21 August 2018 at Brazzaville, Congo. All measurements were confined to 76 male participants (39 specialists vs 37 no specialists of endurance race) in the Brazzaville half-marathon (21.1 km), aged between 19 - 39 years (mean age: 26.7 ± 2.6 years). Coulter profiles with differential white cell counts and haptoglobin levels were determined in venous sample before and after competitive half-marathon race. The same measurements were performed during the 24 hrs following the competition. Results: In the pre-race sample, mild anemia was detected in 12 subjects and mild thrombocytopenia in 7 subjects. Haptoglobin levels were reduced in 5 subjects. Haematological values, all post-race, varied significantly before and after race, particularly for RBC, Hb, Hct, PLT, MCV, MCH, MCHC, WBC, neutrophil counts, lymphocyte counts, monocyte counts, basophil counts, eosinophil counts and haptoglobin. These differences between specialists and no specialists were statistically (p Conclusion: Our data may help sport physicians, sport physiologists and trainers to better follow-up haematological reactions associated with the half-marathon race.展开更多
The blue fox (Vulpes lagopus) is a colour mutation of wild arctic fox. It is commonly farmed for its fur. The aim of the present study was to analyse a baseline biochemical profile of serum from adult farmed blue foxe...The blue fox (Vulpes lagopus) is a colour mutation of wild arctic fox. It is commonly farmed for its fur. The aim of the present study was to analyse a baseline biochemical profile of serum from adult farmed blue foxes during pelting season. Serum biochemical profile can be used later on as a reference in chemical analysis of blood serum. Biochemical profile here consists of liver enzymes alanine aminotransferase (ALAT), aspartatate aminotranasferase (ASAT), alkaline phospahatase (ALP), and total bilirubin (T-BIL);kidney function substances creatinine, urea and inorganic phosphate (Pi);muscle enzyme creatinine kinase (CK);glucose (GLUK) from pancreas;serum total proteins (PROT) and albumin (ALB);electrolytes potassium (K), natrium (Na), chloride (CL), calcium (S-Ca);and lipids such as total cholesterol (T-Cho). For some of the measured parameters reference values were also available from previous studies. Certain values were close to those previously published but some differed markedly. These differences can be explained by differences e.g. in techniques, conditions, unit reports, and breed and age of the animals. The present study additionally reviewed previously published haematological data concerning small blood count, i.e. hemoglobin, hematocrit, white blood cells and red blood cells. Summarized material revealed that these variables are very similar despite of study and animal material. Thus, small blood count can be reliably used to screen general welfare status of farmed blue foxes. The reference values presented in this paper will be valuable as a guide for clinical diagnosis and research.展开更多
Sturgeons are the most important principal market species in the Caspian Sea. In the present study, measurement of blood parameters was carried out with the aim of evaluating the normal value of hematological and seru...Sturgeons are the most important principal market species in the Caspian Sea. In the present study, measurement of blood parameters was carried out with the aim of evaluating the normal value of hematological and serum biochemical parameters of brood stocks Acipenser persicus during spawning season. Blood samples were collected from 36 brood stocks of A. persicus（18 males and 18 females） during the spawning season. Higher value of red blood cells, white blood cells, haemoglobin, haematocrit was observed in male breeders with significant differences between female breeders（p〉0.05）. Haemoglobin and haematocrit value in this study was within the range of 8.70 to 9.2 g/d L and 29.73% to 30.26%, respectively. The statistically significant differences between mean corpuscular volumes, mean corpuscular haemoglobin, lymphocyte and neutrophil percentages, aspartate aminotransferase, alkaline phosphatase and creatine kinase were observed in the male and female brood stocks.It is concluded from this study, some of the hematological and serum biochemical parameters of male and female A. persicus breeders were significantly varied from each other. In comparative investigation with earlier reports,the values of red blood cells, haemoglobin and haematocrit are highly varied due to age variations of fish.However, further studies are necessary to understand the impact of sexual, age, size, length, and season and habitat variation. Serum biochemical parameters can be used for confirming the maturity of A. persicus and monitoring any changes in the water quality parameters and soil.展开更多
The objective of this work was to determine the sodium metabisulphite (NaMBS) subchronic toxicity used as a food additive in Algeria. Three groups of female Wistar rats were treated with 0.25%, 1% and 4% of NaMBS in t...The objective of this work was to determine the sodium metabisulphite (NaMBS) subchronic toxicity used as a food additive in Algeria. Three groups of female Wistar rats were treated with 0.25%, 1% and 4% of NaMBS in their drinking water for 90 days. An immunization protocol was conducted during the experiment. Mortality, comportmental and weight modifications, and food and water consumption were recorded. At the end of the experiment, the control and experiment rats were killed, and their blood and organs were removed. Immunoglobulin levels were evaluated;biochemical and hematological parameters were investigated. Our results showed that the administration of NaMBS at 1% and 4% had significant effects on body weight, food and water consumed. There was an increase in biochemical parameters (calcium, urea, creatinine, uric acid, transaminases) and decrease immunoglobulin levels. The hematology revealed a decrease in red blood cells and hemoglobin, as well as leucocytosis. Physiological study showed enlarged spleen, kidney, liver and stomach. In light of our results, we can conclude that subchronic intake of NaMBS 1% and 4% seems to alter immune function, biochemical, hematological and physiological para-meters in Wistar rats.展开更多
The research aimed to determine the effect of feed supplements of cassava （Manihot esculenta Crantz） leaves, gliricidia （Gliricidia sepeum） leaves and katuk （Sauropus androgynus （L.） Merr.） leaves on the hemat...The research aimed to determine the effect of feed supplements of cassava （Manihot esculenta Crantz） leaves, gliricidia （Gliricidia sepeum） leaves and katuk （Sauropus androgynus （L.） Merr.） leaves on the hematology of lactating buffalo. Four lactating buffaloes in Kanagarian Pamatang Panjang, Sijunjung district, West Sumatera, Indonesia were studied. The experiment used a Latin square design with four treatments and four replications/block. The treatments were control （without supplementary foliage, A）, with cassava leaves （B）, with gliricidia leaves （C） and with katuk leaves （D） each at 5 kg/haead/day. The hematology variables measured were erythrocytes and leucocytes counts, as well as levels of hemoglobin and hematocrit. The results showed that supplementary foliage of 5 kg/head/day of cassava, gliricidia or katuk as leaves significantly increased the erythrocytes count and hemoglobin level, but did not affect the number of leucocytes in lactating buffalo.展开更多
Background: Thioacetamide (TAA) has been used extensively in the development of suitable animal models of acute and chronic liver injury employing various doses, times and routes of its administration, particularly in...Background: Thioacetamide (TAA) has been used extensively in the development of suitable animal models of acute and chronic liver injury employing various doses, times and routes of its administration, particularly in drinking water due to its resemblance with human liver fibrosis and cirrhosis. The aim of this study was to investigate and compare hematological alteration during the acute and chronic liver inflammation. Methods: Acute Liver inflammation was induced in Wistar rats via intraperitoneal injection of thioacetamide and the animals were sacrificed 12 h after the TAA administration. Induction of chronic liver inflammation was performed by continuous administration of TAA in the drinking water (200 mg/L) during 18 weeks of experiment. After that all animals were sacrificed and Blood samples were collected for further analysis. Results: Single intra peritoneal injection of TAA (300 mg/kg B.W.) induced an acute condition with hematological changes including leukocytosis with marked neutrophilia (P = 0.0429), lymphopenia, thrombocytosis as well as increased hemoglobin concentration (P 0.05) and decline of erythrocytic count (P = 0.0009). Eighteen weeks of uninterrupted supply of TAA (200 mg/L) in drinking water lead to chronic inflammation and the hematological alterations were leucopenia (P = 0.0197) accompanied with neutropenia and thrombocytopenia. Increase in RBCs (P = 0.0073) and Hb contents was also observed with a decline of red cell indices. Conclusion: Taken together these findings we can conclude that the animals respond differently under acute and chronic inflammatory condition with TAA administration. Leukocytosis with marked neutrophilia, thrombocytosis as well as increased hemoglobin concentration and decline of erythrocytic count were observed in acute while leucopenia accompanied with neutropenia and thrombocytopenia and increase in RBCs, Hb and Hct was also observed with a decline of other red cell indices during chronic phase.展开更多
文摘BACKGROUND Cervical cancer is a prevalent form of cancer affecting women worldwide and it is the second most common cancer among women in Indonesia,accounting for 8.5%of all cancer-related deaths.Cervical cancer progression can be evaluated through laboratory tests to detect anaemia,an increased platelet count,and elevated inflammatory markers,therefore,effective laboratory examination is crucial for early detection and treatment of cervical cancer.AIM To evaluate the association between laboratory findings(haematology,haematology index,and inflammatory index)and the clinical stage of cervical cancer.METHODS This cross-sectional study analyzed adult cervical cancer patients’data from medical records and laboratory results including sociodemographic status,histopathological finding,clinical stage,and complete haematology examination.Numerical data was analyzed by the one-way ANOVA(normal data distribution),while the Kruskal-Wallis test was used for non-parametric data(abnormal distribution),followed by appropriate post-hoc analysis.The categorical data was analyzed by the Chi-square or Fisher Exact tests.The significance level was established at a P value<0.05.RESULTS This study involved the data of 208 adult cervical cancer patients and found no association between age,marital history,parity history,hormonal contraceptive use and cervical cancer stages.There were significant differences in the clinical laboratory test results based on the clinical stage of cervical cancer,including haemoglobin levels(P<0.001),leucocytes(P<0.001),neutrophils(P<0.001),monocytes(P=0.002),lymphocytes(P=0.006),platelets(P<0.001),neutrophil-lymphocyte ratio/NLR(P<0.001),lymphocyte-monocyte ratio/LMR(P<0.001),and plateletlymphocyte ratio/PLR(P<0.001).There were also significant differences in the systemic inflammatory index(SII)and systematic inflammatory response index(SIRI)between stage III+IV cervical cancer and stage II(SII P<0.001;SIRI P=0.001)and stage I(SII P<0.001;SIRI P=0.016),associated with the shifts in previously mentioned complete haematological values with cancer advancement.CONCLUSION The haematological parameters,inflammatory haematological ratios,and inflammatory indices exhibited significant differences between cervical cancer stages,therefore these tests can be utilized to evaluate cervical cancer progression.
文摘The reduced efficiency of antibiotic growth promoters in livestock, and the unavoidable residual effects on livestock products such as egg, meat and milk have pushed researchers to rush towards the use of phytobiotics in animal feed. This study was conducted at the Teaching and Research Farm of the University of Dschang to evaluate the Effect of <span style="white-space:nowrap;"><i>Cucurma longa</i></span> powder as feed additive on hemato-serological parameters in Guinea pigs. A total of 120 three weeks old Guinea pigs (60 males and 60 females), with an average weight of 150 ± 20 g were used. The animals were randomly distributed into four treatments in a completely randomized design with 30 animals per treatment (15 males and 15 females). Four experimental treatments were used by adding 0%, 0.25%, 0.5% and 1% <i>C. longa</i> in the diet corresponding to R0, R0.25%, R0.5% and R1% respectively. At the 8th week of age, 12 Guinea pigs (6 males and 6 females) per treatment were fasted for 12 hours and sacrificed by cervical dislocation. Blood was rapidly collected from the jugular vein for the evaluation of haematological and biochemical parameters. Results revealed that the inclusion of <span style="white-space:normal;"><i></span>Curcuma longa<span style="white-space:normal;"></i></span> at 0.25% significantly (p < 0.05) increased the concentration of hematocrit, lymphocytes, monocytes, granulocytes and platelets. A significant decrease (p < 0.05) was observed in hemoglobin, red blood cells, white blood cells, serum lipids, globulin, AST, ALT, and urea. It was concluded that the use of <span style="white-space:normal;"><i></span>Curcuma longa<span style="white-space:normal;"></i></span> powder at 0.25% as feed additive positively regulates blood serum parameters of Guinea pigs.
文摘Background: Adequate selection of a prospective whole blood donor protects his health and safety of the recipient. Objectives: The main objective of this study was to determine the haematology parameters of apparently healthy prospective whole blood donors. Participants and Methods: This was a hospital based prospective study carried out from August to October 2020 at the blood transfusion unit of the Lagos State University Teaching Hospital (LASUTH), Ikeja, Nigeria. A structured pretested questionnaire was used for data collection. The socio demographic status and the haematology parameters of apparently healthy prospective whole blood donors who tested negative for HIV, hepatitis B and C markers were captured. Obtained data were analysed with the statistical package for the social scientist software version 20. Results: One hundred male (97.1%) and three female (2.9%) apparently healthy prospective whole blood donors were studied. The median age of study subjects was 30 years. Obtained median haematology parameter values were 13 g/dl, 40%, 4.9/nl and 203.9/nl for haemoglobin concentration, haematocrit, total white cell and platelet counts respectively. The median values for the mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) of participants were 32.6 g/dl, 27.7 pg and 85.7 fl respectively. Observed prevalence of subnormal haematology parameters for haemoglobin concentration, total white cells, platelets were 12.6%, 25.2%, and 13.6% respectively. Also subnormal values for MCHC, MCH, MCV were 11.7%, 26.2%, and 16.5% respectively among prospective whole blood donors in this study. No higher than normal haematology parameter values were observed. Median values for erythrocyte sedimentation rate was 8.4 mm/hr. Conclusion: A significant percentage of apparently healthy prospective whole blood donors had subnormal haematology parameters values. Obtained normal values in our study are comparable with local reference range reports from previous studies in Nigeria and other parts of Africa. 124947 .
文摘Basic haematological parameters were studied in adult, sexually mature individuals of colour morphs (striata and maculata) in the populations of Pelophylax ridibundus living in biotopes with various degrees of anthropogenic pollution (control, domestic sewage pollution and heavy metal pollution) in Southern Bulgaria. We found that in the polluted biotopes in individuals of both morphs of P. ridibundus the blood parameters: erythrocyte (RBC) and leukocyte (WBS) count, haemoglobin concentration (Hb), haematocrit (PCV) were statistically reliably higher, the number of lymphocytes (Ly) decreased, and the parameters: mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC), mean cell volume (MCV) and differential blood formula changed considerably in comparison with the control group. In terms of anthropogenic pollution, the average values of RBC and Hb were higher in P. ridibundus of striata morph.
基金financial support from Health and Welfare Sector Education and Training Authority towards this projectthe funding received from the North-West University Ph D bursary
文摘The present study investigated the effect of partial replacement of soybean meal(Glycine max) with canola meal(CM)(Brassica napus) on the growth performance,haematology,serum biochemistry and meat quality characteristics of female Japanese quails in a 35-day feeding trial.One hundred and forty6-week-old quails 158.28 ± 11.919 g were randomly allocated to 5 isonitrogenous and isoenergetic experimental diets: control diet(CM0; with no CM inclusion); CM0 with 2.5%(CM25),5.0%(CM50),12.5%(CM125) and 17.5%(CM175) soybean meal replaced with CM.Average weekly gain(AWG) and feed conversion efficiency(FCE) were determined.Haematology,serum biochemistry,carcass traits and meat quality parameters were determined at slaughter.Quails fed CM175 had the lowest(P < 0.05) feed intake whereas no differences were observed among the other 4 diets.No dietary effects on AWG,FCE and haematological parameters were observed.Serum biochemical parameters were not influenced by diets with the exception of alkaline phosphatase(ALP),where quails fed CM25 had higher ALP(161.0 U/L) than those fed CM0(37.25 U/L).Quails fed CM25 had the highest chroma(7.39) while those fed CM125 had the lowest(3.58) at 24 h post-slaughter.Diets had no influence(P > 0.05) on cooking losses and peak positive force of quail meat.It was concluded that CM can replace soybean in quail diets up to 12.5% without compromising growth performance,health and quality of meat.Inclusion levels beyond 12.5% promoted poor voluntary feed intake and thus may require feed additives to enhance utilization.
基金supported by management of Al Ain Wildlife Park and Resort(Grant No.12/917002).
文摘Objective:To investigate the haematology and biochemistry values of apparently healthy captive sand cats kept in Al Ain Wildlife Park and Resort,United Arab Emirates,with a view to establishing baseline values.Methods:Blood was collected from the femoral vein using aseptic techniques,kept in a cool box and sent to laboratory for analysis.The blood was analysed for haematological and biochemical values using veterinary hematology and chemistry analysers(ABX ABC Vet,Horiba ABX SAS^(®)Montpellier,France).Results:Haematological values were within the normal ranges recorded in domestic cats and there was no statistical difference between values found in males and females.Aspartate aminotransferase values were higher(P<0.05)in males compared to female cats.Serum protein,creatinine and glucose values for females were significantly(P<0.05)higher than those for males.Creatinine kinase,urea and lactate dehydrogenase levels were higher than those reported for normal domestic cat,but there was no statistical difference(P>0.05)between males and females values.Conclusions:Our results present reference ranges for haematology and biochemistry parameters in captive sand cats.These values will be important for diagnosis of various diseases and monitoring of treatments.
基金Supported by The Chongqing Health Commission and Science and Technology Bureau,No.2023MSXM060.
文摘We specifically discuss the mechanisms of the pathogenesis,diagnosis,and management of blastic plasmacytoid dendritic cell neoplasm(BPDCN),a rare but aggressive haematologic malignancy characterized by frequent skin manifestations and systemic dissemination.The article enriches our understanding of BPDCN through detailed case reports showing the clinical,immunophenotypic,and histopathological features that are critical for diagnosing this disease.These cases highlight the essential role of pathologists in employing advanced immunophenotyping techniques to accurately identify the disease early in its course and guide treatment decisions.Furthermore,we explore the implications of these findings for management strategies,emphasizing the use of targeted therapies such as tagraxofusp and the potential of allogeneic haematopoietic stem cell transplantation in achieving remission.The editorial underscores the importance of interdisciplinary approaches in managing BPDCN,pointing towards a future where precision medicine could significantly improve patient outcomes.
文摘The study aimed to investigate the Haematological and Serum Biochemical indices of finisher broiler chickens fed graded levels of Euphorbia heterophylla leaf meal (EHLM) also known as spurge weed. The birds were allotted into six dietary treatments of ten birds segregated into three replicates each. The diets formulated with EHLM were included at 0%, 5%, 10%, 15%, 20% and 25% levels in diets 1, 2, 3, 4, 5 and 6 respectively to replace soybean. Each treatment was replicated three times in a completely randomized design. Uncoagulated blood samples were collected from the birds at the end of the 56 days feeding trial and analysed for packed cell volume (PCV), haemoglobin concentrate (Hb), red blood cells (RBC) and white blood cells (WBC). The mean corpuscular haemoglobin volume (MCV), mean corpuscular haemoglobin (MCH), platelets, neutrophils, lymphocytes, monocytes, eosinophils, and basophils were calculated using PCV, RBC and Hb. The blood meant for serological analysis was centrifuged at 1000 G for 10 minutes, after which the serum was separated and used for determining serum total protein (Tp), Albumin, Serum glutamic oxaloacetic transaminase (SGOT) and Serum glutamic pyruvic transaminase (SGPT). The results revealed that the control group had significantly higher values of PCV, RBC, and Hb compared to other treatment groups. However, the values of MCV, MCH, lymphocytes, heterophils, and eosinophils were similar to the control. The biochemical parameters showed significant differences among treatment groups, but not significantly different from the control. The study concluded that EHLM may not pose a health challenge to broiler chickens at levels of 5 - 15 percent, but improved health, immunity and performance can be achieved at the 15% inclusion level.
文摘Due to its toxicity, dichlorvos—a common organophosphate pesticide—poses significant risks to human health. This study utilized male Wistar rats to explore the potential protective effects of L-arginine supplementation against dichlorvos-induced toxicity, focusing on cardiotoxicity, haematotoxicity and oxidative stress. The rats were divided into four groups: Control, L-arginine (L), Dichlorvos (D), and L-arginine + Dichlorvos (L + D). Dichlorvos was administered to the D group, L-arginine (100 mg/kg) to the L group, and both L-arginine and dichlorvos to the L + D group. The study evaluated various parameters, including cardiovascular, oxidative stress markers, and haematological indices. Significant changes in haematological parameters such as haemoglobin (Hb), haematocrit (HCT), and red blood cell count (RBC) indicated haematotoxicity after dichlorvos administration. Additionally, elevated cardiac markers, including lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB), suggested cardiotoxic effects. Exposure to dichlorvos also resulted in decreased antioxidant enzyme levels and increased oxidative stress indicators like malondialdehyde (MDA). Remarkably, L-arginine supplementation mitigated the damage caused by dichlorvos. It normalized the altered haematological parameters, demonstrating its protective effect against haematotoxicity. The rise in cardiac markers was reduced with L-arginine supplementation, indicating protection against cardiotoxicity. Moreover, L-arginine significantly decreased oxidative stress, as evidenced by lower MDA levels and restored antioxidant enzyme activity. In conclusion, L-arginine supplementation in male Wistar rats showed promising protective effects against dichlorvos-induced cardiotoxicity, haematotoxicity and oxidative stress. This suggests that L-arginine may offer a beneficial intervention to mitigate the adverse effects of dichlorvos on blood and heart health, paving the way for potential treatments for pesticide poisoning.
文摘Objective:To investigate the antidiabetic properties of aqueous extract of stem bark of Afzelia africana(A.africana)and its beneficial effect on haematological parameters in streptozotocin induced diabetic rats.Methods:A total of 30 rats including 24 diabetic and 6 normal rats were used for this study.Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin.After being confirmed diabetic,animals were orally treated with distilled water or extracts at 100 or 200 mg/kg body weight daily for 10 days.The haematological parameters including red blood and white blood cells and their functional indices were evaluated in diabetic treated groups compared with the controls.Results:The extract significantly reduced the blood glucose levels while the best result was obtained at 200 mg/kg body weight The feed and water intake in diabetic rats were significantly reduced while weight loss was minimized at both dosages.Similarly,the levels of red blood,white blood cells and their functional indices were significantly improved after extract administration at both doses.Conclusions:It can be concluded that the aqueous extract of bark of A.africana possesses antihyperglycemic properties.In addition,the extract can prevent various complications of diabetes and improve some haematological parameters.Further experimental investigation is needed to exploit its relevant therapeutic effect to substantiate its ethnomedicinal usage.
基金Supported by Directorate of Research,Guru Angad Dev Veterinary and Animal Sciences University,Ludhiana-141004,India.Grant No.-RKVYB-2(Ⅲ)c(d)DR-2,sanctioned on October,2010
文摘Objective:To evaluate clinical and hematological aspects of dogs naturally infected with Hepatozoon canis(H.canis)presented at the Small Animal Clinics of Guru Angad Dev Veterinary and Animal Sciences University,Ludhiana.Methods:Blood films of 34 naturally infected dogs were examined for haematological alterations and parasitaemia.Signalment and clinical signs were recorded from the animals.Clinical histories were filled oul during the consultation.Results:Of the 34 positive dogs by Giemsa stained peripheral blood films.88.23%presented parasilaemia by H.canis only,while 11.77%had the combination of H.canis,Babesia sp.and Ehrlichia sp.Young male dogs less than oue-year-old,of nondescript breed,were the most commonly affected.And 26.47%were presented with anorexia/inappetence as the only clinical symptom.Other clinical symptoms were mild to moderate fever,pale mucosae and lethargy;a few were also showing the signs of vomiting and diarrhoea.Haematological alterations showed mainly normochromic-normocytic anaemia,leukocytosis and neutrophilia.Conclusions:The findings of this study substantiate that H.canis caused clinical and haematological alterations of the varied intensity in dogs,even with low parasilaemia.should he taken into consideration.
文摘Objective:To explore cytotoxic activity of ethanol extract of Alpinia calcarata Rose(EEAC) rhizome against Ehiiich ascites carcinoma(EAC) tumor bearing Swiss Albino mice.Methods: In the present study,its anti-neoplastic activity has been studied by monitoring parameters like tumor weight measurement,survival time,tumor cell growth inhibition,haematological characteristics etc.Results:It was found that EEAC at dose 8 mg/kg/day(i.p.) significantly decreased tumor weight(62.0%;P【0.01),increased life span(70.25%;P 【0.01) and reduced tumor cell growth rate(85.7%;P【0.01) in comparison to those of EAC bearing mice.The plant extract also improved the depleted haematological parameters like RBC WBC,Hb%,differential counts(e.g.lymphocytes,neutrophils,monocytes etc) of EAC bearing mice towards normal.The host toxic effects were not very high and recovered gradually towards normal within a few days after treatment.ConchiskHis:EEAC exhibits potent in vivo cytotoxic activity against EAC tumor bearing Swiss Albino mice.So,the plant can be considered as a probable new source of antitumor agents.
文摘To investigate the effects ofMoringa oleifera leaf meal （MOLM） on the performance and haematology of broiler finisher using 120 Cobb chicks of 4 weeks old broiler chicks. The birds were assigned randomly into four dietary treatments containing MOLM at 0, 6%, 8%, and 10% （treatments 1, 2, 3 and 4） inclusion levels, respectively, in a complete randomized design experiment. The effect of the dietary treatments on the growth performance and blood parameters of the broiler finisher was determined. MOLM at a rate of 6% （T2）, 8% （T3）, and 10% （T4） of the diets （as fed basis） to replace 3.2%, 5.6% and 8.6% of the crude protein （CP） of the control diet. The daily feed, dry matter and CP intake of the chicks fed MOLM diets were higher （p〈0.05） than those fed the control diet. Average weight gain （AWG） of birds fed MOLM diets reduced as inclusion levels increases 09〈0.05） than those fed the control diet. Chicks fed T2 showed higher （p〈0.05） AWG than those on T3 and T4. Feed conversion ratios （FCR, weight gain/g and feed intake） were higher for chicks fed MOLM. MOLM showed minimal deleterious effects in birds. However, birds fed T2 （6%） diets recorded significantly （p〈0.05） the highest body weight gain. There were significant differences （p〈0.05） among groups in packed cell volume （PVC） of birds. The haemoglobin （Hb）, mean corpuscular volume （MCV）, mean corpuscular haemoglobin （MCH） and mean corpuscular haemoglobin concentration （MCHC） counts showed no significant difference （p〉0.05） among treatments. The results indicated that at 10% in the diets of broiler finisher chick, MOLM could be substituted with expensive conventional protein sources without any deleterious effects on performance and blood parameters of broiler finisher chicken.
文摘Bt corn “MON810: Ajeeb YG” produces delta endotoxins in the whole plant due to the genetic modification process. The chemical analysis of this variety showed significant differences from its conventional counterpart “Ajeeb”. Further, feeding studies on rats were designed to complete assessing the safety of “MON810: Ajeeb YG”. Three groups of rats (6 males and 6 females’ rats/group) were fed on control diet, non-Bt corn and Bt corn for 1.5 months and 3 months. After 1.5 months, 3 males and 3 females from each group were sacrificed, and after another 1.5 months, 3 males and 3 females from each group and their offspring were sacrificed. Histopathological examination, blood haematology and serum biochemical analysis were determined. Results indicated significant differences among the tested parameters in the three groups especially Bt group. Severe changes in the liver of Bt group after 3 months were observed.
文摘Background: Measurement of haematological parameters has been historically helpful in the diagnosis of many diseases in endurance sportsmen. The modifications of these parameters during endurance race have not yet been evaluated in many African countries. Objectives: To determine haematological values before and immediately after a half-marathon event, as well as within 24 hours after the race and to analyze the changes observed. Methods: A cross-sectional study was conducted from 10 to 21 August 2018 at Brazzaville, Congo. All measurements were confined to 76 male participants (39 specialists vs 37 no specialists of endurance race) in the Brazzaville half-marathon (21.1 km), aged between 19 - 39 years (mean age: 26.7 ± 2.6 years). Coulter profiles with differential white cell counts and haptoglobin levels were determined in venous sample before and after competitive half-marathon race. The same measurements were performed during the 24 hrs following the competition. Results: In the pre-race sample, mild anemia was detected in 12 subjects and mild thrombocytopenia in 7 subjects. Haptoglobin levels were reduced in 5 subjects. Haematological values, all post-race, varied significantly before and after race, particularly for RBC, Hb, Hct, PLT, MCV, MCH, MCHC, WBC, neutrophil counts, lymphocyte counts, monocyte counts, basophil counts, eosinophil counts and haptoglobin. These differences between specialists and no specialists were statistically (p Conclusion: Our data may help sport physicians, sport physiologists and trainers to better follow-up haematological reactions associated with the half-marathon race.
文摘The blue fox (Vulpes lagopus) is a colour mutation of wild arctic fox. It is commonly farmed for its fur. The aim of the present study was to analyse a baseline biochemical profile of serum from adult farmed blue foxes during pelting season. Serum biochemical profile can be used later on as a reference in chemical analysis of blood serum. Biochemical profile here consists of liver enzymes alanine aminotransferase (ALAT), aspartatate aminotranasferase (ASAT), alkaline phospahatase (ALP), and total bilirubin (T-BIL);kidney function substances creatinine, urea and inorganic phosphate (Pi);muscle enzyme creatinine kinase (CK);glucose (GLUK) from pancreas;serum total proteins (PROT) and albumin (ALB);electrolytes potassium (K), natrium (Na), chloride (CL), calcium (S-Ca);and lipids such as total cholesterol (T-Cho). For some of the measured parameters reference values were also available from previous studies. Certain values were close to those previously published but some differed markedly. These differences can be explained by differences e.g. in techniques, conditions, unit reports, and breed and age of the animals. The present study additionally reviewed previously published haematological data concerning small blood count, i.e. hemoglobin, hematocrit, white blood cells and red blood cells. Summarized material revealed that these variables are very similar despite of study and animal material. Thus, small blood count can be reliably used to screen general welfare status of farmed blue foxes. The reference values presented in this paper will be valuable as a guide for clinical diagnosis and research.
基金supported by Iranian Fisheries Research OrganizationTehran University
文摘Sturgeons are the most important principal market species in the Caspian Sea. In the present study, measurement of blood parameters was carried out with the aim of evaluating the normal value of hematological and serum biochemical parameters of brood stocks Acipenser persicus during spawning season. Blood samples were collected from 36 brood stocks of A. persicus（18 males and 18 females） during the spawning season. Higher value of red blood cells, white blood cells, haemoglobin, haematocrit was observed in male breeders with significant differences between female breeders（p〉0.05）. Haemoglobin and haematocrit value in this study was within the range of 8.70 to 9.2 g/d L and 29.73% to 30.26%, respectively. The statistically significant differences between mean corpuscular volumes, mean corpuscular haemoglobin, lymphocyte and neutrophil percentages, aspartate aminotransferase, alkaline phosphatase and creatine kinase were observed in the male and female brood stocks.It is concluded from this study, some of the hematological and serum biochemical parameters of male and female A. persicus breeders were significantly varied from each other. In comparative investigation with earlier reports,the values of red blood cells, haemoglobin and haematocrit are highly varied due to age variations of fish.However, further studies are necessary to understand the impact of sexual, age, size, length, and season and habitat variation. Serum biochemical parameters can be used for confirming the maturity of A. persicus and monitoring any changes in the water quality parameters and soil.
文摘The objective of this work was to determine the sodium metabisulphite (NaMBS) subchronic toxicity used as a food additive in Algeria. Three groups of female Wistar rats were treated with 0.25%, 1% and 4% of NaMBS in their drinking water for 90 days. An immunization protocol was conducted during the experiment. Mortality, comportmental and weight modifications, and food and water consumption were recorded. At the end of the experiment, the control and experiment rats were killed, and their blood and organs were removed. Immunoglobulin levels were evaluated;biochemical and hematological parameters were investigated. Our results showed that the administration of NaMBS at 1% and 4% had significant effects on body weight, food and water consumed. There was an increase in biochemical parameters (calcium, urea, creatinine, uric acid, transaminases) and decrease immunoglobulin levels. The hematology revealed a decrease in red blood cells and hemoglobin, as well as leucocytosis. Physiological study showed enlarged spleen, kidney, liver and stomach. In light of our results, we can conclude that subchronic intake of NaMBS 1% and 4% seems to alter immune function, biochemical, hematological and physiological para-meters in Wistar rats.
文摘The research aimed to determine the effect of feed supplements of cassava （Manihot esculenta Crantz） leaves, gliricidia （Gliricidia sepeum） leaves and katuk （Sauropus androgynus （L.） Merr.） leaves on the hematology of lactating buffalo. Four lactating buffaloes in Kanagarian Pamatang Panjang, Sijunjung district, West Sumatera, Indonesia were studied. The experiment used a Latin square design with four treatments and four replications/block. The treatments were control （without supplementary foliage, A）, with cassava leaves （B）, with gliricidia leaves （C） and with katuk leaves （D） each at 5 kg/haead/day. The hematology variables measured were erythrocytes and leucocytes counts, as well as levels of hemoglobin and hematocrit. The results showed that supplementary foliage of 5 kg/head/day of cassava, gliricidia or katuk as leaves significantly increased the erythrocytes count and hemoglobin level, but did not affect the number of leucocytes in lactating buffalo.
文摘Background: Thioacetamide (TAA) has been used extensively in the development of suitable animal models of acute and chronic liver injury employing various doses, times and routes of its administration, particularly in drinking water due to its resemblance with human liver fibrosis and cirrhosis. The aim of this study was to investigate and compare hematological alteration during the acute and chronic liver inflammation. Methods: Acute Liver inflammation was induced in Wistar rats via intraperitoneal injection of thioacetamide and the animals were sacrificed 12 h after the TAA administration. Induction of chronic liver inflammation was performed by continuous administration of TAA in the drinking water (200 mg/L) during 18 weeks of experiment. After that all animals were sacrificed and Blood samples were collected for further analysis. Results: Single intra peritoneal injection of TAA (300 mg/kg B.W.) induced an acute condition with hematological changes including leukocytosis with marked neutrophilia (P = 0.0429), lymphopenia, thrombocytosis as well as increased hemoglobin concentration (P 0.05) and decline of erythrocytic count (P = 0.0009). Eighteen weeks of uninterrupted supply of TAA (200 mg/L) in drinking water lead to chronic inflammation and the hematological alterations were leucopenia (P = 0.0197) accompanied with neutropenia and thrombocytopenia. Increase in RBCs (P = 0.0073) and Hb contents was also observed with a decline of red cell indices. Conclusion: Taken together these findings we can conclude that the animals respond differently under acute and chronic inflammatory condition with TAA administration. Leukocytosis with marked neutrophilia, thrombocytosis as well as increased hemoglobin concentration and decline of erythrocytic count were observed in acute while leucopenia accompanied with neutropenia and thrombocytopenia and increase in RBCs, Hb and Hct was also observed with a decline of other red cell indices during chronic phase.
