Biphasic calcium phosphate （BCP） nanopowders were synthesized by using microwave and non-microwave irradiation assisted processes. The synthesized powders were pressed under a pressure of 90 MPa, and then were sinte...Biphasic calcium phosphate （BCP） nanopowders were synthesized by using microwave and non-microwave irradiation assisted processes. The synthesized powders were pressed under a pressure of 90 MPa, and then were sintered at 1000-1200℃ for 1 h. The mechanical properties of the samples were investigated. The formed phases and microstructures were characterized by X-ray diffraction （XRD） and scanning electron microscopy （SEM）. The results showed that the synthesis time was shorter, along with a more homogeneous microstructure, when the microwave irradiation assisted method was applied. The compression strength and the Young＇s modulus of the samples synthesized with microwave irradiation were about 60 MPa and 3 GPa, but those of the samples synthesized without microwave irradiation were about 30 MPa and 2 GPa, respectively. XRD patterns of the microwave irradiation assisted and non-microwave irradiation assisted nanopowders showed the coexistence of hydroxyapatite （HA） and lricalcium phosphate （TCP） phases in the system.展开更多
目的研究羟基磷灰石/磷酸三钙(hydroxyapatite/tricalcium phosphate,HA/TCP)双相生物陶瓷与重组人骨形态发生蛋白-2(recombinant human bone morphogentic protein-2,rhBMP-2)的复合材料对骨质疏松骨缺损的修复作用。方法选用30只8个...目的研究羟基磷灰石/磷酸三钙(hydroxyapatite/tricalcium phosphate,HA/TCP)双相生物陶瓷与重组人骨形态发生蛋白-2(recombinant human bone morphogentic protein-2,rhBMP-2)的复合材料对骨质疏松骨缺损的修复作用。方法选用30只8个月龄雌性SD大鼠,随机分为两组。实验组(20只)行双侧卵巢摘除术,对照组(10只)行假手术。术后12周确定实验组骨质疏松模型建立成功,两组大鼠双侧股骨外侧髁上造成直径3 mm骨缺损。同时实验组随机植骨分为两组:单纯HA/TCP组和rhBMP-2/HA-TCP复合材料组;对照组全部植入rhBMP-2/HA-TCP复合材料。植骨术后4、8和12周取双侧股骨标本,进行组织学、X射线、骨密度及生物力学检测。结果实验HA/TCP组4周时材料周围存在密度减低区,缺损部位新生骨量明显低于实验复合材料组,12周时新生骨板界面可见类骨质,材料吸收较快;实验复合材料组12周时材料大部分降解转化为新骨,可见成熟的骨小梁和骨髓组织,有编织骨形成。与单纯HA/TCP组相比,4、8周时实验复合材料组缺损部位X射线、骨密度以及生物力学值明显提高(P<0.05),与对照复合材料组比较差异无显著性(P>0.05)。结论HA/TCP双相生物陶瓷是rhBMP-2较理想的载体材料,复合后具有良好的骨诱导作用,能够明显促进骨质疏松骨缺损的修复。展开更多
用化学沉淀法制备β-磷酸钙/羟基磷灰石双向陶瓷(β-TCP/HA)粉末。采用粉末冶金法,以(HA/β-TCP)粉末和Ti粉末为原料,经1050℃真空烧结60 min制备出含不同质量分数钛粉的(HA/β-TCP)/Ti复合材料:(β-TCP/HA)/60wt%Ti、(β-TCP/HA)/70wt...用化学沉淀法制备β-磷酸钙/羟基磷灰石双向陶瓷(β-TCP/HA)粉末。采用粉末冶金法,以(HA/β-TCP)粉末和Ti粉末为原料,经1050℃真空烧结60 min制备出含不同质量分数钛粉的(HA/β-TCP)/Ti复合材料:(β-TCP/HA)/60wt%Ti、(β-TCP/HA)/70wt%Ti、(β-TCP/HA)/80wt%Ti。用扫描电子显微镜(SEM)和X射线衍射仪(XRD)对复合材料进行了组织结构和物相成分表征,并采用维氏显微硬度计测试了复合材料的显微硬度。采用模拟体液浸泡(SPF)进行体外生物活性表征,结果表明:复合材料的物相主要为α-Ti、TiO_2、Ca5(PO4)3(OH)、β-Ca3(PO4)2和Ca Ti O3;块状陶瓷相沿富Ti相等形成的网状结构的孔隙中分布。(β-TCP/HA)/60wt%Ti复合材料中Ca5(PO4)3(OH)分解程度最小,显微硬度为HV539,最接近人体骨骼的硬度。经14天SPF溶液浸泡后,Ca5(PO4)3(OH)的衍射峰沿(211)晶面具有明显的择优取向,材料表面密布结晶度良好的棒状Ca5(PO4)3(OH)晶体。展开更多
文摘Biphasic calcium phosphate （BCP） nanopowders were synthesized by using microwave and non-microwave irradiation assisted processes. The synthesized powders were pressed under a pressure of 90 MPa, and then were sintered at 1000-1200℃ for 1 h. The mechanical properties of the samples were investigated. The formed phases and microstructures were characterized by X-ray diffraction （XRD） and scanning electron microscopy （SEM）. The results showed that the synthesis time was shorter, along with a more homogeneous microstructure, when the microwave irradiation assisted method was applied. The compression strength and the Young＇s modulus of the samples synthesized with microwave irradiation were about 60 MPa and 3 GPa, but those of the samples synthesized without microwave irradiation were about 30 MPa and 2 GPa, respectively. XRD patterns of the microwave irradiation assisted and non-microwave irradiation assisted nanopowders showed the coexistence of hydroxyapatite （HA） and lricalcium phosphate （TCP） phases in the system.
文摘目的研究羟基磷灰石/磷酸三钙(hydroxyapatite/tricalcium phosphate,HA/TCP)双相生物陶瓷与重组人骨形态发生蛋白-2(recombinant human bone morphogentic protein-2,rhBMP-2)的复合材料对骨质疏松骨缺损的修复作用。方法选用30只8个月龄雌性SD大鼠,随机分为两组。实验组(20只)行双侧卵巢摘除术,对照组(10只)行假手术。术后12周确定实验组骨质疏松模型建立成功,两组大鼠双侧股骨外侧髁上造成直径3 mm骨缺损。同时实验组随机植骨分为两组:单纯HA/TCP组和rhBMP-2/HA-TCP复合材料组;对照组全部植入rhBMP-2/HA-TCP复合材料。植骨术后4、8和12周取双侧股骨标本,进行组织学、X射线、骨密度及生物力学检测。结果实验HA/TCP组4周时材料周围存在密度减低区,缺损部位新生骨量明显低于实验复合材料组,12周时新生骨板界面可见类骨质,材料吸收较快;实验复合材料组12周时材料大部分降解转化为新骨,可见成熟的骨小梁和骨髓组织,有编织骨形成。与单纯HA/TCP组相比,4、8周时实验复合材料组缺损部位X射线、骨密度以及生物力学值明显提高(P<0.05),与对照复合材料组比较差异无显著性(P>0.05)。结论HA/TCP双相生物陶瓷是rhBMP-2较理想的载体材料,复合后具有良好的骨诱导作用,能够明显促进骨质疏松骨缺损的修复。
文摘用化学沉淀法制备β-磷酸钙/羟基磷灰石双向陶瓷(β-TCP/HA)粉末。采用粉末冶金法,以(HA/β-TCP)粉末和Ti粉末为原料,经1050℃真空烧结60 min制备出含不同质量分数钛粉的(HA/β-TCP)/Ti复合材料:(β-TCP/HA)/60wt%Ti、(β-TCP/HA)/70wt%Ti、(β-TCP/HA)/80wt%Ti。用扫描电子显微镜(SEM)和X射线衍射仪(XRD)对复合材料进行了组织结构和物相成分表征,并采用维氏显微硬度计测试了复合材料的显微硬度。采用模拟体液浸泡(SPF)进行体外生物活性表征,结果表明:复合材料的物相主要为α-Ti、TiO_2、Ca5(PO4)3(OH)、β-Ca3(PO4)2和Ca Ti O3;块状陶瓷相沿富Ti相等形成的网状结构的孔隙中分布。(β-TCP/HA)/60wt%Ti复合材料中Ca5(PO4)3(OH)分解程度最小,显微硬度为HV539,最接近人体骨骼的硬度。经14天SPF溶液浸泡后,Ca5(PO4)3(OH)的衍射峰沿(211)晶面具有明显的择优取向,材料表面密布结晶度良好的棒状Ca5(PO4)3(OH)晶体。
