[ Objective] This paper aimed to investigate the origin, characteristics and molecular evolution of duck derived H4N6 subtype avian influ- enza virus （DK/SH/Y20/06） and enrich the epidemiologic data of the waterfowl...[ Objective] This paper aimed to investigate the origin, characteristics and molecular evolution of duck derived H4N6 subtype avian influ- enza virus （DK/SH/Y20/06） and enrich the epidemiologic data of the waterfowl origin AIV. [Method] The entire genome of DK/SH/Y20/06 was amplified and subjected to genome sequencing. The molecular software was used for sequence analysis and phylogenetic tree construction of DK/ SH/Y20/06 with some other reference sequences in GenBank. [Result] The results indicated that the amino acid sequence adjacent to HA cleav- age site was PEKASR ↓ GLF, which was the typical characteristics of the LPAIV. The phylogenetic analysis indicated that the HA gene of the isolate was derived from the Eurasian lineage in the eastern hemisphere. The NA gene was at the same branch with A/rnallard/Yan chen/2005（ H4N6）, sharing 98.3% sequence identity. The PB2, PB1, NP and PA gene of this isolate had genetically close relationships with H6 subtype AIV which is epidemic in China at present. The M gene fell into the same branch with A/environment/Korea/CSM05/2004（ H3N1 ）. The NS segment had the highest similarity with A/wild duck/Korea/YS44/2004（H1N2）. The eight genes were not at the same branch and shared a low similarity with other H4N6 subtype avian influenza viruses isolated in North America. [Condusion] These data showed that DK/SH/Y20/06（H4N6） was possibly a re- combinant virus derived from H4N6 subtype, H6N2, H6N5, H3N1 and H1 N2 subtype AIV by complex gene recombination in duck.展开更多
水禽源的H4N6病毒株能否感染鸡或者其他禽类,以及致病力如何尚未清楚,本研究利用2018年1月初从健康水禽监测样品中分离到的1株H4N6亚型AIV,命名为A/Duck/Henan/JM/2018(H4N6),对其HA基因遗传特点及对SPF鸡和樱桃谷鸭的感染特点进行了系...水禽源的H4N6病毒株能否感染鸡或者其他禽类,以及致病力如何尚未清楚,本研究利用2018年1月初从健康水禽监测样品中分离到的1株H4N6亚型AIV,命名为A/Duck/Henan/JM/2018(H4N6),对其HA基因遗传特点及对SPF鸡和樱桃谷鸭的感染特点进行了系统研究。试验结果显示,JM株的HA基因裂解位点为PEKASR I GLF,符合低致病性禽流感病毒的分子特征,属于欧亚分支,与国内的H4N6毒株亲缘关系较近,表明该毒株的HA基因变异不大;JM株感染鸡和鸭后可通过消化道和呼吸道有效地向外界排毒,说明病毒可以在体内增殖,且有一定的水平传播能力,揭示病毒有跨宿主传播的可能。展开更多
In order to investigate the Magnetic Behavior of Compounds,the Variable —temperature magnetic susceptibility data of the title compounds(I:[Cu(C6H8O4)(C7H6N2)2H2O]∞) and(II:[Cu(C4H4O4)(C7H6N2)2 H2O]∞) were recorded...In order to investigate the Magnetic Behavior of Compounds,the Variable —temperature magnetic susceptibility data of the title compounds(I:[Cu(C6H8O4)(C7H6N2)2H2O]∞) and(II:[Cu(C4H4O4)(C7H6N2)2 H2O]∞) were recorded and discussed quantitatively based on to Ising’s model and particle swarm optimization(PSO).J≈-1.3cm-1,which shows that there is a weak interaction between two Cu(II) ions in the title compounds.It can be interpreted with Kahn’s approximate molecular orbital model.Room-temperature magnetic moment(2.150μB) is larger than pure-spin magnetic moment(1.732μB) and g is larger than ge(2.002),which comfirms the existence of spin-orbit coupling in the compounds.展开更多
基金supported by Science and Technology Commission of Shanghai Municipality Biomedicine Major Projects(09DZ1906602)
文摘[ Objective] This paper aimed to investigate the origin, characteristics and molecular evolution of duck derived H4N6 subtype avian influ- enza virus （DK/SH/Y20/06） and enrich the epidemiologic data of the waterfowl origin AIV. [Method] The entire genome of DK/SH/Y20/06 was amplified and subjected to genome sequencing. The molecular software was used for sequence analysis and phylogenetic tree construction of DK/ SH/Y20/06 with some other reference sequences in GenBank. [Result] The results indicated that the amino acid sequence adjacent to HA cleav- age site was PEKASR ↓ GLF, which was the typical characteristics of the LPAIV. The phylogenetic analysis indicated that the HA gene of the isolate was derived from the Eurasian lineage in the eastern hemisphere. The NA gene was at the same branch with A/rnallard/Yan chen/2005（ H4N6）, sharing 98.3% sequence identity. The PB2, PB1, NP and PA gene of this isolate had genetically close relationships with H6 subtype AIV which is epidemic in China at present. The M gene fell into the same branch with A/environment/Korea/CSM05/2004（ H3N1 ）. The NS segment had the highest similarity with A/wild duck/Korea/YS44/2004（H1N2）. The eight genes were not at the same branch and shared a low similarity with other H4N6 subtype avian influenza viruses isolated in North America. [Condusion] These data showed that DK/SH/Y20/06（H4N6） was possibly a re- combinant virus derived from H4N6 subtype, H6N2, H6N5, H3N1 and H1 N2 subtype AIV by complex gene recombination in duck.
文摘水禽源的H4N6病毒株能否感染鸡或者其他禽类,以及致病力如何尚未清楚,本研究利用2018年1月初从健康水禽监测样品中分离到的1株H4N6亚型AIV,命名为A/Duck/Henan/JM/2018(H4N6),对其HA基因遗传特点及对SPF鸡和樱桃谷鸭的感染特点进行了系统研究。试验结果显示,JM株的HA基因裂解位点为PEKASR I GLF,符合低致病性禽流感病毒的分子特征,属于欧亚分支,与国内的H4N6毒株亲缘关系较近,表明该毒株的HA基因变异不大;JM株感染鸡和鸭后可通过消化道和呼吸道有效地向外界排毒,说明病毒可以在体内增殖,且有一定的水平传播能力,揭示病毒有跨宿主传播的可能。
文摘In order to investigate the Magnetic Behavior of Compounds,the Variable —temperature magnetic susceptibility data of the title compounds(I:[Cu(C6H8O4)(C7H6N2)2H2O]∞) and(II:[Cu(C4H4O4)(C7H6N2)2 H2O]∞) were recorded and discussed quantitatively based on to Ising’s model and particle swarm optimization(PSO).J≈-1.3cm-1,which shows that there is a weak interaction between two Cu(II) ions in the title compounds.It can be interpreted with Kahn’s approximate molecular orbital model.Room-temperature magnetic moment(2.150μB) is larger than pure-spin magnetic moment(1.732μB) and g is larger than ge(2.002),which comfirms the existence of spin-orbit coupling in the compounds.
