Histone methylation is involved in a wide range of biological regulation in plants,and is conducted by three major components,including methyltransferases,demethylases,and histone readers.Compared with the other two c...Histone methylation is involved in a wide range of biological regulation in plants,and is conducted by three major components,including methyltransferases,demethylases,and histone readers.Compared with the other two components,research on histone readers is relatively limited.In this study,we demonstrate that OsSHH5 functions as an H3K9me1 reader to regulate rice disease resistance,tillering,and grain yield.Loss of OsSHH5 function significantly enhances both grain yield and disease resistance.Mechanistically,OsSHH5 recruits the H3K9 methyltransferase SGD733 and binds to H3K9me1,thereby maintaining H3K9me1 enrichment and facilitating gene silencing.In leaves,OsSHH5 interacts with the transcriptional factor HPY1 to target the resistance-related genes OsWAKg52 and OsWRKY81,maintaining their H3K9me1 levels and suppressing multiple PAMP-triggered immune responses,which ultimately reduces rice disease resistance.In tiller buds,OsSHH5 interacts with the transcriptional factor TCP19 to target the tillering-related gene OsNGR5,maintaining its H3K9me1 enrichment and inhibition of tillering,leading to reduced yield.Collectively,these findings reveal that OsSHH5 plays a vital role in integrating immune response,tillering,and grain yield in rice,providing new insights into the function of histone readers and offering a new strategy to improve rice yield and disease resistance.展开更多
目的研究分析胎膜早破(PROM)并发绒毛膜羊膜炎患者胎盘组织中组蛋白H3第4位赖氨酸三甲基化(H3K4me3)、组蛋白H3第27位赖氨酸三甲基化(H3K27me3)及修饰酶的表达水平及其临床意义。方法回顾性收集2021年6月~2023年12月因PROM在西安高新医...目的研究分析胎膜早破(PROM)并发绒毛膜羊膜炎患者胎盘组织中组蛋白H3第4位赖氨酸三甲基化(H3K4me3)、组蛋白H3第27位赖氨酸三甲基化(H3K27me3)及修饰酶的表达水平及其临床意义。方法回顾性收集2021年6月~2023年12月因PROM在西安高新医院就诊的95例孕妇临床资料,根据是否并发组织学绒毛膜羊膜炎(HCA)分为感染组(n=41)和非感染组(n=54);另选取同期30例正常妊娠产妇作为对照组。取所有受试者胎盘组织,检测H3K4me3,H3K27me3的表达水平;实时荧光定量PCR(qRT-PCR)检测H3K4me3,H3K27me3特异性甲基化转移酶[混合谱系白血病蛋白1(MLL1),Zeste增强子同源物2(EZH2)]和去甲基化转移酶[赖氨酸特异性去甲基化酶5B(KDM5B),Jumonji结构域包含蛋白3(JMJD3)]水平。分析三组H3K4me3,H3K27me3及特异性修饰酶表达差异及与HCA组织学分期的相关性;绘制受试者工作特征(ROC)曲线,计算曲线下面积(AUC)值,评估H3K4me3,H3K27me3对并发HCA感染的预测价值。结果与对照组相比,感染组和非感染组H3K4me3(0.08%±0.02%,0.12%±0.03%vs 0.25%±0.06%)及MLL1 mRNA(1.32±0.16,1.44±0.23 vs 2.02±0.31),JMJD3 mRNA(0.78±0.13,0.91±0.15 vs 1.53±0.23)水平明显降低(t=10.939~19.062),H3K27me39(0.23%±0.05%,0.15%±0.03%vs 0.10%±0.02%)及EZH2 mRNA(1.96±0.26,1.85±0.25 vs 1.15±0.29),KDM5B mRNA(1.46±0.15,1.35±0.18 vs 0.94±0.12)水平明显升高(t=6.077~14.974),且感染组各水平低于/高于非感染组(t=2.017~10.688),差异具有统计学意义(均P<0.05)。PROM胎盘组织中H3K4me3与H3K27me3水平呈显著负相关(r=-0.604,P<0.05);H3K4me3,MLL1,JMJD3 mRNA水平分别与HCA组织学分期呈负相关(r=-0.646,-0.489,-0.503,均P<0.05);H3K27me3,EZH2 mRNA,KDM5B mRNA水平分别与HCA组织学分期呈正相关(r=0.632,0.515,0.520,均P<0.05)。当H3K4me3,H3K27me3截断值分别取0.10%,0.19%时,两者联合预测PROM并发HCA感染的AUC(95%CI)值为0.896(0.882~0.947),敏感度、特异度分别为92.14%和86.23%,明显高于两者单一指标诊断。结论PROM胎盘组织中H3K4me3,H3K27me3及修饰酶水平与HCA感染及其组织学分期密切相关,对并发HCA感染具有较高的临床预测价值,有望作为临床诊断的新型生物标志物。展开更多
基金supported by the National Natural Science Foundation of China(32470391,32401801,and 31870322)the Open Competitive Project of the Wuhan East Lake high-tech Zone(2023KJB220)the Key Research and Development Program of Hubei Province(2025BBA002).
文摘Histone methylation is involved in a wide range of biological regulation in plants,and is conducted by three major components,including methyltransferases,demethylases,and histone readers.Compared with the other two components,research on histone readers is relatively limited.In this study,we demonstrate that OsSHH5 functions as an H3K9me1 reader to regulate rice disease resistance,tillering,and grain yield.Loss of OsSHH5 function significantly enhances both grain yield and disease resistance.Mechanistically,OsSHH5 recruits the H3K9 methyltransferase SGD733 and binds to H3K9me1,thereby maintaining H3K9me1 enrichment and facilitating gene silencing.In leaves,OsSHH5 interacts with the transcriptional factor HPY1 to target the resistance-related genes OsWAKg52 and OsWRKY81,maintaining their H3K9me1 levels and suppressing multiple PAMP-triggered immune responses,which ultimately reduces rice disease resistance.In tiller buds,OsSHH5 interacts with the transcriptional factor TCP19 to target the tillering-related gene OsNGR5,maintaining its H3K9me1 enrichment and inhibition of tillering,leading to reduced yield.Collectively,these findings reveal that OsSHH5 plays a vital role in integrating immune response,tillering,and grain yield in rice,providing new insights into the function of histone readers and offering a new strategy to improve rice yield and disease resistance.
文摘目的研究分析胎膜早破(PROM)并发绒毛膜羊膜炎患者胎盘组织中组蛋白H3第4位赖氨酸三甲基化(H3K4me3)、组蛋白H3第27位赖氨酸三甲基化(H3K27me3)及修饰酶的表达水平及其临床意义。方法回顾性收集2021年6月~2023年12月因PROM在西安高新医院就诊的95例孕妇临床资料,根据是否并发组织学绒毛膜羊膜炎(HCA)分为感染组(n=41)和非感染组(n=54);另选取同期30例正常妊娠产妇作为对照组。取所有受试者胎盘组织,检测H3K4me3,H3K27me3的表达水平;实时荧光定量PCR(qRT-PCR)检测H3K4me3,H3K27me3特异性甲基化转移酶[混合谱系白血病蛋白1(MLL1),Zeste增强子同源物2(EZH2)]和去甲基化转移酶[赖氨酸特异性去甲基化酶5B(KDM5B),Jumonji结构域包含蛋白3(JMJD3)]水平。分析三组H3K4me3,H3K27me3及特异性修饰酶表达差异及与HCA组织学分期的相关性;绘制受试者工作特征(ROC)曲线,计算曲线下面积(AUC)值,评估H3K4me3,H3K27me3对并发HCA感染的预测价值。结果与对照组相比,感染组和非感染组H3K4me3(0.08%±0.02%,0.12%±0.03%vs 0.25%±0.06%)及MLL1 mRNA(1.32±0.16,1.44±0.23 vs 2.02±0.31),JMJD3 mRNA(0.78±0.13,0.91±0.15 vs 1.53±0.23)水平明显降低(t=10.939~19.062),H3K27me39(0.23%±0.05%,0.15%±0.03%vs 0.10%±0.02%)及EZH2 mRNA(1.96±0.26,1.85±0.25 vs 1.15±0.29),KDM5B mRNA(1.46±0.15,1.35±0.18 vs 0.94±0.12)水平明显升高(t=6.077~14.974),且感染组各水平低于/高于非感染组(t=2.017~10.688),差异具有统计学意义(均P<0.05)。PROM胎盘组织中H3K4me3与H3K27me3水平呈显著负相关(r=-0.604,P<0.05);H3K4me3,MLL1,JMJD3 mRNA水平分别与HCA组织学分期呈负相关(r=-0.646,-0.489,-0.503,均P<0.05);H3K27me3,EZH2 mRNA,KDM5B mRNA水平分别与HCA组织学分期呈正相关(r=0.632,0.515,0.520,均P<0.05)。当H3K4me3,H3K27me3截断值分别取0.10%,0.19%时,两者联合预测PROM并发HCA感染的AUC(95%CI)值为0.896(0.882~0.947),敏感度、特异度分别为92.14%和86.23%,明显高于两者单一指标诊断。结论PROM胎盘组织中H3K4me3,H3K27me3及修饰酶水平与HCA感染及其组织学分期密切相关,对并发HCA感染具有较高的临床预测价值,有望作为临床诊断的新型生物标志物。
