针对含光伏(photovoltaic,PV)、电动汽车(electric vehicle,EV)及家庭电器负荷的智能社区,以车入户(vehicle to home,V2H)的形式将EV纳入家庭需求响应框架,利用EV的双向输能特性并考虑EV充/放电带来的电池容量退化成本,协同PV、电网的...针对含光伏(photovoltaic,PV)、电动汽车(electric vehicle,EV)及家庭电器负荷的智能社区,以车入户(vehicle to home,V2H)的形式将EV纳入家庭需求响应框架,利用EV的双向输能特性并考虑EV充/放电带来的电池容量退化成本,协同PV、电网的实时电价和用户需求的可容忍时延,基于Lyapunov优化理论提出随机环境下V2H用户的EV充/放电调度策略和每户家庭的负荷响应策略,最小化家庭用户的长期平均购电成本。并提出一种智能社区在线能量交易方案,旨在最小化智能社区总的购电成本、最大限度提高社区能源利用率。理论分析和仿真结果表明,所提算法无需实时电价、PV出力、用户负荷需求的先验概率信息,仅基于当前系统状态就可使优化目标趋于最优值,实现家庭用户的能量调度和家庭用户之间的能量共享,减少家庭购电成本,提高用户之间能量交易的灵活性。展开更多
为确定H9N2猪流感病毒(H9N2-SIV)通过瞬时受体电位通道M2(TRPM2)介导肺微血管上皮细胞(PMVEC)铁死亡的分子机制,使用H9N2-SIV接种PMVEC,构建TRPM2-siRNA质粒并转染细胞。用透射电镜观察细胞超微结构,用荧光探针法检测活性氧(ROS)、Ca^(...为确定H9N2猪流感病毒(H9N2-SIV)通过瞬时受体电位通道M2(TRPM2)介导肺微血管上皮细胞(PMVEC)铁死亡的分子机制,使用H9N2-SIV接种PMVEC,构建TRPM2-siRNA质粒并转染细胞。用透射电镜观察细胞超微结构,用荧光探针法检测活性氧(ROS)、Ca^(2+)和Fe^(2+);用生化试剂盒检测丙二醛(MDA)和谷胱甘肽(GSH)含量,并通过荧光定量PCR和Western-blot检测葡萄糖调节蛋白78(GRP78)、TRPM2、蛋白激酶R样内质网激酶(PERK)、活化转录因子4(ATF4)、阳离子转运调控样蛋白1(CHAC1)、谷胱甘肽过氧化物酶4(GPX4)的m RNA和蛋白表达水平。结果显示,H9N2-SIV感染可诱导细胞铁死亡,敲低TRPM2可以减少细胞内ROS水平,降低Ca^(2+)、Fe^(2+)及MDA含量,GSH水平明显增加;此外,GRP78、PERK、ATF4、CHAC1 m RNA和蛋白表达水平下调,GPX4的m RNA和蛋白表达水平上调。结果表明,H9N2-SIV感染可诱导细胞铁死亡,其可通过激活TRPM2使Ca^(2+)内流增多,进而激活PERK/ATF4/CHAC1信号通路,加速GSH耗竭,抑制GPX4的活性,促进细胞铁死亡。展开更多
为了探讨了H2H(Hospital To Home)营养管理模式下的持续营养补充对食管癌患者术后营养状况及生活质量的影响,选取了在胸外科首次确诊为食管癌并接受手术治疗的81例患者为研究对象,随机分为实验组和对照组。对照组实行常规营养管理,实验...为了探讨了H2H(Hospital To Home)营养管理模式下的持续营养补充对食管癌患者术后营养状况及生活质量的影响,选取了在胸外科首次确诊为食管癌并接受手术治疗的81例患者为研究对象,随机分为实验组和对照组。对照组实行常规营养管理,实验组采取H2H营养管理模式进行持续营养补充。结果显示,两组患者术后血红蛋白浓度均有不同程度下降,且实验组术后3 d的血红蛋白浓度低于对照组(P=0.037)。术后3 d的血清白蛋白(38.2 g·L^(-1))及前白蛋白(256.7 g·L^(-1))浓度实验组均高于对照组(36.3、180.9 g·L^(-1),均有P<0.05)。两组患者术后总蛋白和前白蛋白浓度均有下降,出院后逐渐回升,实验组的总体趋势高于对照组。对生活质量进行评估,实验组术后6个月的躯体功能、情绪功能及总体健康水平得分均显著高于对照组(81.7 vs 70.2,85.5 vs 75.0,73.0 vs 62.8,均有P<0.05)。综上,H2H营养管理模式下的持续营养补充可改善食管癌患者术后营养状况,提高生活质量,有助于改善预后。H2H营养管理模式可为肿瘤患者术后长期营养管理提供参考模式。展开更多
目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微...目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微镜观察细胞形态变化,并通过油红O染色、免疫荧光法、线粒体探针法以及线粒体电镜技术对分化细胞进行鉴定分析。结果:未分化的C3H10T1/2细胞形态多样,具有突触伸展特征;分化后的细胞逐渐变为圆形或椭圆形,形成环形脂滴;未分化组细胞形态无明显变化。油红O染色显示,未分化组细胞基本无染色,而分化组中约90%的细胞红染,脂滴分布于细胞核周围,吸光度值显著升高(P<0.05)。分化组解偶联蛋白1(uncoupling protein 1,UCP1)、过氧化物酶体增殖激活受体γ(peroxisome proliferator-activated receptorγ,PPARγ)辅激活因子1α(PPARγco-activator-1α,PGC-1α)、PPARγ和转录因子PRDM16的相对荧光强度和蛋白表达量显著高于未分化组(P<0.05),且线粒体活性增强。结论:本研究成功诱导C3H10T1/2细胞分化为成熟棕色脂肪细胞,结合细胞形态观察、关键蛋白表达检测及线粒体功能分析进行综合评估,证实了细胞的有效分化及成熟棕色脂肪细胞的功能特性。展开更多
Construction of metal-mediated redox sites is an appealing approach to enhance photocatalytic CO_(2)reduction coupled with H_(2)O oxidation.However,conventional static redox sites generally lack spatiotemporal matchin...Construction of metal-mediated redox sites is an appealing approach to enhance photocatalytic CO_(2)reduction coupled with H_(2)O oxidation.However,conventional static redox sites generally lack spatiotemporal matching during reaction processes due to the constraints of rigid structure and the linear scaling relationship of adsorbed species.Herein,an alkanolamine-Ir synergistic system was developed,where flexible monoethanolamine(MEA)molecules function as molecular ferries to selectively adsorb CO_(2)via carbamate formation,while adjacent Ir nanoparticles(NPs)serve as H spillover hubs that relay protons,creating spatiotemporal adaptability that synchronizes CO_(2)reduction and water oxidation.In addition,time-resolved in situ spectroscopy directly captures the rapid transformation of carbamate intermediates concurrent with sustained IrOOH intermediates formation.Microkinetic modeling further demonstrates that the MEA-Ir modified system(M-Ir/ACN)creates interconnected H spillover networks between Ir NPs and MEA,facilitating efficient proton transport that drives^(*)COOH formation with a favorable thermodynamic energy.As a result,the M-Ir/ACN achieves a 20-fold increase in CO production compared to the pristine sample while maintaining high stability throughout 45 h of continuous operation.This study presents that flexible molecular ferries boost CO_(2)adsorption,and deciphers how flexible molecular-metal synergy directs the trafficking of CO_(2)-derived intermediates toward highly efficient CO_(2)photoreduction.展开更多
Metal hydrides with high hydrogen density provide promising hydrogen storage paths for hydrogen transportation.However,the requirement of highly pure H_(2)for re-hydrogenation limits its wide application.Here,amorphou...Metal hydrides with high hydrogen density provide promising hydrogen storage paths for hydrogen transportation.However,the requirement of highly pure H_(2)for re-hydrogenation limits its wide application.Here,amorphous Al_(2)O_(3)shells(10 nm)were deposited on the surface of highly active hydrogen storage material particles(MgH_(2)-ZrTi)by atomic layer deposition to obtain MgH_(2)-ZrTi@Al_(2)O_(3),which have been demonstrated to be air stable with selective adsorption of H_(2)under a hydrogen atmosphere with different impurities(CH_(4),O_(2),N_(2),and CO_(2)).About 4.79 wt% H_(2)was adsorbed by MgH_(2)-ZrTi@10nmAl_(2)O_(3)at 75℃under 10%CH_(4)+90%H_(2)atmosphere within 3 h with no kinetic or density decay after 5 cycles(~100%capacity retention).Furthermore,about 4 wt%of H_(2)was absorbed by MgH_(2)-ZrTi@10nmAl_(2)O_(3)under 0.1%O_(2)+0.4%N_(2)+99.5%H_(2)and 0.1%CO_(2)+0.4%N_(2)+99.5%H_(2)atmospheres at 100℃within 0.5 h,respectively,demonstrating the selective hydrogen absorption of MgH_(2)-ZrTi@10nmAl_(2)O_(3)in both oxygen-containing and carbon dioxide-containing atmospheres hydrogen atmosphere.The absorption and desorption curves of MgH_(2)-ZrTi@10nmAl_(2)O_(3)with and without absorption in pure hydrogen and then in 21%O_(2)+79%N_(2)for 1 h were found to overlap,further confirming the successful shielding effect of Al_(2)O_(3)shells against O_(2)and N_(2).The MgH_(2)-ZrTi@10nmAl_(2)O_(3)has been demonstrated to be air stable and have excellent selective hydrogen absorption performance under the atmosphere with CH_(4),O_(2),N_(2),and CO_(2).展开更多
目的:探讨餐后2 h血糖(2 h PG)、糖化血红蛋白(HbA1c)、空腹血糖(FPG)、在妊娠期糖尿病(GDM)孕妇中表达情况及其与母婴结局的关系。方法:回顾性分析2022年1月至2024年12月在漯河市第二人民医院产检的150例孕妇资料,按照是否发生GDM分为...目的:探讨餐后2 h血糖(2 h PG)、糖化血红蛋白(HbA1c)、空腹血糖(FPG)、在妊娠期糖尿病(GDM)孕妇中表达情况及其与母婴结局的关系。方法:回顾性分析2022年1月至2024年12月在漯河市第二人民医院产检的150例孕妇资料,按照是否发生GDM分为两组,其中75例发生GDM孕妇纳入研究组,75例未发生GDM孕妇纳入对照组。检测两组2 h PG、FPG、HbA1c,比较两组各指标检测值差异及不良妊娠结局、围生儿结局发生情况,分析2 h PG、FPG、HbA1c与GDM孕妇母婴结局的相关性。结果:研究组孕妇2 h PG检测值为(10.05±1.10)mmol/L、FPG检测值为(6.91±0.63)mmol/L、HbA1c检测值为(5.91±0.63)%,均高于对照组(P<0.05);与对照组相比,研究组胎儿窘迫、妊娠期高血压疾病(HDCP)、早产、产后出血、胎膜早破发生率较高(P<0.05),Spearman分析显示,GDM孕妇FPG、2 h PG、HbA1c水平与胎儿窘迫、HDCP、早产、胎膜早破、产后出血发生呈正相关性(P<0.05);研究组新生儿高胆红素血症(NHB)、巨大儿、新生儿低血糖症、新生儿窒息发生率高于对照组(P<0.05),Spearman分析显示,2 h PG、FPG、HbA1c与巨大儿、NHB、新生儿窒息、新生儿低血糖症呈正相关性(P<0.05)。结论:GDM孕妇2 h PG、FPG、HbA1c异常升高,其水平与GDM孕妇不良母婴结局发生密切相关。展开更多
文摘针对含光伏(photovoltaic,PV)、电动汽车(electric vehicle,EV)及家庭电器负荷的智能社区,以车入户(vehicle to home,V2H)的形式将EV纳入家庭需求响应框架,利用EV的双向输能特性并考虑EV充/放电带来的电池容量退化成本,协同PV、电网的实时电价和用户需求的可容忍时延,基于Lyapunov优化理论提出随机环境下V2H用户的EV充/放电调度策略和每户家庭的负荷响应策略,最小化家庭用户的长期平均购电成本。并提出一种智能社区在线能量交易方案,旨在最小化智能社区总的购电成本、最大限度提高社区能源利用率。理论分析和仿真结果表明,所提算法无需实时电价、PV出力、用户负荷需求的先验概率信息,仅基于当前系统状态就可使优化目标趋于最优值,实现家庭用户的能量调度和家庭用户之间的能量共享,减少家庭购电成本,提高用户之间能量交易的灵活性。
文摘为确定H9N2猪流感病毒(H9N2-SIV)通过瞬时受体电位通道M2(TRPM2)介导肺微血管上皮细胞(PMVEC)铁死亡的分子机制,使用H9N2-SIV接种PMVEC,构建TRPM2-siRNA质粒并转染细胞。用透射电镜观察细胞超微结构,用荧光探针法检测活性氧(ROS)、Ca^(2+)和Fe^(2+);用生化试剂盒检测丙二醛(MDA)和谷胱甘肽(GSH)含量,并通过荧光定量PCR和Western-blot检测葡萄糖调节蛋白78(GRP78)、TRPM2、蛋白激酶R样内质网激酶(PERK)、活化转录因子4(ATF4)、阳离子转运调控样蛋白1(CHAC1)、谷胱甘肽过氧化物酶4(GPX4)的m RNA和蛋白表达水平。结果显示,H9N2-SIV感染可诱导细胞铁死亡,敲低TRPM2可以减少细胞内ROS水平,降低Ca^(2+)、Fe^(2+)及MDA含量,GSH水平明显增加;此外,GRP78、PERK、ATF4、CHAC1 m RNA和蛋白表达水平下调,GPX4的m RNA和蛋白表达水平上调。结果表明,H9N2-SIV感染可诱导细胞铁死亡,其可通过激活TRPM2使Ca^(2+)内流增多,进而激活PERK/ATF4/CHAC1信号通路,加速GSH耗竭,抑制GPX4的活性,促进细胞铁死亡。
文摘为了探讨了H2H(Hospital To Home)营养管理模式下的持续营养补充对食管癌患者术后营养状况及生活质量的影响,选取了在胸外科首次确诊为食管癌并接受手术治疗的81例患者为研究对象,随机分为实验组和对照组。对照组实行常规营养管理,实验组采取H2H营养管理模式进行持续营养补充。结果显示,两组患者术后血红蛋白浓度均有不同程度下降,且实验组术后3 d的血红蛋白浓度低于对照组(P=0.037)。术后3 d的血清白蛋白(38.2 g·L^(-1))及前白蛋白(256.7 g·L^(-1))浓度实验组均高于对照组(36.3、180.9 g·L^(-1),均有P<0.05)。两组患者术后总蛋白和前白蛋白浓度均有下降,出院后逐渐回升,实验组的总体趋势高于对照组。对生活质量进行评估,实验组术后6个月的躯体功能、情绪功能及总体健康水平得分均显著高于对照组(81.7 vs 70.2,85.5 vs 75.0,73.0 vs 62.8,均有P<0.05)。综上,H2H营养管理模式下的持续营养补充可改善食管癌患者术后营养状况,提高生活质量,有助于改善预后。H2H营养管理模式可为肿瘤患者术后长期营养管理提供参考模式。
文摘目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微镜观察细胞形态变化,并通过油红O染色、免疫荧光法、线粒体探针法以及线粒体电镜技术对分化细胞进行鉴定分析。结果:未分化的C3H10T1/2细胞形态多样,具有突触伸展特征;分化后的细胞逐渐变为圆形或椭圆形,形成环形脂滴;未分化组细胞形态无明显变化。油红O染色显示,未分化组细胞基本无染色,而分化组中约90%的细胞红染,脂滴分布于细胞核周围,吸光度值显著升高(P<0.05)。分化组解偶联蛋白1(uncoupling protein 1,UCP1)、过氧化物酶体增殖激活受体γ(peroxisome proliferator-activated receptorγ,PPARγ)辅激活因子1α(PPARγco-activator-1α,PGC-1α)、PPARγ和转录因子PRDM16的相对荧光强度和蛋白表达量显著高于未分化组(P<0.05),且线粒体活性增强。结论:本研究成功诱导C3H10T1/2细胞分化为成熟棕色脂肪细胞,结合细胞形态观察、关键蛋白表达检测及线粒体功能分析进行综合评估,证实了细胞的有效分化及成熟棕色脂肪细胞的功能特性。
基金supported by the National Natural Science Foundation of China(22422502,22209076)the Natural Science Foundation of Jiangsu Province(BK 20220369)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(1020242193)。
文摘Construction of metal-mediated redox sites is an appealing approach to enhance photocatalytic CO_(2)reduction coupled with H_(2)O oxidation.However,conventional static redox sites generally lack spatiotemporal matching during reaction processes due to the constraints of rigid structure and the linear scaling relationship of adsorbed species.Herein,an alkanolamine-Ir synergistic system was developed,where flexible monoethanolamine(MEA)molecules function as molecular ferries to selectively adsorb CO_(2)via carbamate formation,while adjacent Ir nanoparticles(NPs)serve as H spillover hubs that relay protons,creating spatiotemporal adaptability that synchronizes CO_(2)reduction and water oxidation.In addition,time-resolved in situ spectroscopy directly captures the rapid transformation of carbamate intermediates concurrent with sustained IrOOH intermediates formation.Microkinetic modeling further demonstrates that the MEA-Ir modified system(M-Ir/ACN)creates interconnected H spillover networks between Ir NPs and MEA,facilitating efficient proton transport that drives^(*)COOH formation with a favorable thermodynamic energy.As a result,the M-Ir/ACN achieves a 20-fold increase in CO production compared to the pristine sample while maintaining high stability throughout 45 h of continuous operation.This study presents that flexible molecular ferries boost CO_(2)adsorption,and deciphers how flexible molecular-metal synergy directs the trafficking of CO_(2)-derived intermediates toward highly efficient CO_(2)photoreduction.
基金supported by the National Natural Science Foundation of China(22175136)the State Key Laboratory of Electrical Insulation and Power Equipment(EIPE23127)the Fundamental Research Funds for the Central Universities(xtr052024009).
文摘Metal hydrides with high hydrogen density provide promising hydrogen storage paths for hydrogen transportation.However,the requirement of highly pure H_(2)for re-hydrogenation limits its wide application.Here,amorphous Al_(2)O_(3)shells(10 nm)were deposited on the surface of highly active hydrogen storage material particles(MgH_(2)-ZrTi)by atomic layer deposition to obtain MgH_(2)-ZrTi@Al_(2)O_(3),which have been demonstrated to be air stable with selective adsorption of H_(2)under a hydrogen atmosphere with different impurities(CH_(4),O_(2),N_(2),and CO_(2)).About 4.79 wt% H_(2)was adsorbed by MgH_(2)-ZrTi@10nmAl_(2)O_(3)at 75℃under 10%CH_(4)+90%H_(2)atmosphere within 3 h with no kinetic or density decay after 5 cycles(~100%capacity retention).Furthermore,about 4 wt%of H_(2)was absorbed by MgH_(2)-ZrTi@10nmAl_(2)O_(3)under 0.1%O_(2)+0.4%N_(2)+99.5%H_(2)and 0.1%CO_(2)+0.4%N_(2)+99.5%H_(2)atmospheres at 100℃within 0.5 h,respectively,demonstrating the selective hydrogen absorption of MgH_(2)-ZrTi@10nmAl_(2)O_(3)in both oxygen-containing and carbon dioxide-containing atmospheres hydrogen atmosphere.The absorption and desorption curves of MgH_(2)-ZrTi@10nmAl_(2)O_(3)with and without absorption in pure hydrogen and then in 21%O_(2)+79%N_(2)for 1 h were found to overlap,further confirming the successful shielding effect of Al_(2)O_(3)shells against O_(2)and N_(2).The MgH_(2)-ZrTi@10nmAl_(2)O_(3)has been demonstrated to be air stable and have excellent selective hydrogen absorption performance under the atmosphere with CH_(4),O_(2),N_(2),and CO_(2).
文摘目的:探讨餐后2 h血糖(2 h PG)、糖化血红蛋白(HbA1c)、空腹血糖(FPG)、在妊娠期糖尿病(GDM)孕妇中表达情况及其与母婴结局的关系。方法:回顾性分析2022年1月至2024年12月在漯河市第二人民医院产检的150例孕妇资料,按照是否发生GDM分为两组,其中75例发生GDM孕妇纳入研究组,75例未发生GDM孕妇纳入对照组。检测两组2 h PG、FPG、HbA1c,比较两组各指标检测值差异及不良妊娠结局、围生儿结局发生情况,分析2 h PG、FPG、HbA1c与GDM孕妇母婴结局的相关性。结果:研究组孕妇2 h PG检测值为(10.05±1.10)mmol/L、FPG检测值为(6.91±0.63)mmol/L、HbA1c检测值为(5.91±0.63)%,均高于对照组(P<0.05);与对照组相比,研究组胎儿窘迫、妊娠期高血压疾病(HDCP)、早产、产后出血、胎膜早破发生率较高(P<0.05),Spearman分析显示,GDM孕妇FPG、2 h PG、HbA1c水平与胎儿窘迫、HDCP、早产、胎膜早破、产后出血发生呈正相关性(P<0.05);研究组新生儿高胆红素血症(NHB)、巨大儿、新生儿低血糖症、新生儿窒息发生率高于对照组(P<0.05),Spearman分析显示,2 h PG、FPG、HbA1c与巨大儿、NHB、新生儿窒息、新生儿低血糖症呈正相关性(P<0.05)。结论:GDM孕妇2 h PG、FPG、HbA1c异常升高,其水平与GDM孕妇不良母婴结局发生密切相关。
