目的了解广东省甲型H1N1pdm流感病毒聚合酶碱性蛋白2(polymerase basic protein 2,PB2)基因变异株的分子特征,探讨其特异性分子位点,为流感病毒防控提供科学依据。方法采集感染PB2基因变异株的2例病例咽拭子标本进行病毒分离,并挑选23...目的了解广东省甲型H1N1pdm流感病毒聚合酶碱性蛋白2(polymerase basic protein 2,PB2)基因变异株的分子特征,探讨其特异性分子位点,为流感病毒防控提供科学依据。方法采集感染PB2基因变异株的2例病例咽拭子标本进行病毒分离,并挑选23株广东省流感病毒株进行测序分析,利用GISAID提供的参考序列和疫苗株序列对血凝素(hemagglutinin,HA)和PB2基因进行进化分析;开展毒株抗原分析和神经氨酸酶(neuraminidase,NA)抑制试验;构建PB2蛋白模型,分析聚合酶活性。结果PB2-D701N和PB2-A271S变异株HA基因出现H399N氨基酸位点突变,均属于6B.1A.5a.2a分支;与疫苗株A/Victoria/4897/2022同属大分支不同小分支,均为疫苗类似株。在三维结构中,PB2-D701N和PB2-A271S突变发生电荷改变和亲疏水变化。结论PB2-D701和A271高度保守,PB2突变株尚未成为优势株,但其抗原性与疫苗匹配性高,对NA抑制剂敏感。三维模型推测,PB2-D701N突变可增强病毒的致病力而影响传播能力,PB2-A271S则可能影响流感病毒的聚合酶活性和聚合酶复合物的合成。展开更多
Influenza viruses are common respiratory pathogens in humans and can cause serious infection that leads to the development of pneumonia.Due to their hostrange diversity,genetic and antigenic diversity,and potential to...Influenza viruses are common respiratory pathogens in humans and can cause serious infection that leads to the development of pneumonia.Due to their hostrange diversity,genetic and antigenic diversity,and potential to reassort genetically in vivo,influenza A viruses are continual sources of novel influenza strains that lead to the emergence of periodic epidemics and outbreaks in humans.Thus,newly emerging viral diseases are always major threats to public health.In March 2009,a novel influenza virus suddenly emerged and caused a worldwide pandemic.The novel pandemic influenza virus was genetically and antigenically distinct from previous seasonal human influenza A/H1N1 viruses;it was identified to have originated from pigs,and further genetic analysis revealed it as a subtype of A/H1N1,thus later called a swine-origin influenza virus A/H1N1.Since the novel virus emerged,epidemiological surveys and research on experimental animal models have been conducted,and characteristics of the novel influenza virus have been determined but the exact mechanisms of pulmonary pathogenesis remain to be elucidated.In this editorial,we summa-rize and discuss the recent pandemic caused by the novel swine-origin influenza virus A/H1N1 with a focus on the mechanism of pathogenesis to obtain an insight into potential therapeutic strategies.展开更多
The Influenza A(H1N1)pdm09 virus caused a global pandemic in 2009 and has circulated seasonally ever since.As the continual genetic evolution of hemagglutinin in this virus leads to antigenic drift,rapid identificatio...The Influenza A(H1N1)pdm09 virus caused a global pandemic in 2009 and has circulated seasonally ever since.As the continual genetic evolution of hemagglutinin in this virus leads to antigenic drift,rapid identification of antigenic variants and characterization of the antigenic evolution are needed.In this study,we developed PREDAC-H1pdm,a model to predict antigenic relationships between H1N1pdm viruses and identify antigenic clusters for post-2009 pandemic H1N1 strains.Our model performed well in predicting antigenic variants,which was helpful in influenza surveillance.By mapping the antigenic clusters for H1N1pdm,we found that substitutions on the Sa epitope were common for H1N1pdm,whereas for the former seasonal H1N1,substitutions on the Sb epitope were more common in antigenic evolution.Additionally,the localized epidemic pattern of H1N1pdm was more obvious than that of the former seasonal H1N1,which could make vaccine recommendation more sophisticated.Overall,the antigenic relationship prediction model we developed provides a rapid determination method for identifying antigenic variants,and the further analysis of evolutionary and epidemic characteristics can facilitate vaccine recommendations and influenza surveillance for H1N1pdm.展开更多
文摘目的了解广东省甲型H1N1pdm流感病毒聚合酶碱性蛋白2(polymerase basic protein 2,PB2)基因变异株的分子特征,探讨其特异性分子位点,为流感病毒防控提供科学依据。方法采集感染PB2基因变异株的2例病例咽拭子标本进行病毒分离,并挑选23株广东省流感病毒株进行测序分析,利用GISAID提供的参考序列和疫苗株序列对血凝素(hemagglutinin,HA)和PB2基因进行进化分析;开展毒株抗原分析和神经氨酸酶(neuraminidase,NA)抑制试验;构建PB2蛋白模型,分析聚合酶活性。结果PB2-D701N和PB2-A271S变异株HA基因出现H399N氨基酸位点突变,均属于6B.1A.5a.2a分支;与疫苗株A/Victoria/4897/2022同属大分支不同小分支,均为疫苗类似株。在三维结构中,PB2-D701N和PB2-A271S突变发生电荷改变和亲疏水变化。结论PB2-D701和A271高度保守,PB2突变株尚未成为优势株,但其抗原性与疫苗匹配性高,对NA抑制剂敏感。三维模型推测,PB2-D701N突变可增强病毒的致病力而影响传播能力,PB2-A271S则可能影响流感病毒的聚合酶活性和聚合酶复合物的合成。
文摘Influenza viruses are common respiratory pathogens in humans and can cause serious infection that leads to the development of pneumonia.Due to their hostrange diversity,genetic and antigenic diversity,and potential to reassort genetically in vivo,influenza A viruses are continual sources of novel influenza strains that lead to the emergence of periodic epidemics and outbreaks in humans.Thus,newly emerging viral diseases are always major threats to public health.In March 2009,a novel influenza virus suddenly emerged and caused a worldwide pandemic.The novel pandemic influenza virus was genetically and antigenically distinct from previous seasonal human influenza A/H1N1 viruses;it was identified to have originated from pigs,and further genetic analysis revealed it as a subtype of A/H1N1,thus later called a swine-origin influenza virus A/H1N1.Since the novel virus emerged,epidemiological surveys and research on experimental animal models have been conducted,and characteristics of the novel influenza virus have been determined but the exact mechanisms of pulmonary pathogenesis remain to be elucidated.In this editorial,we summa-rize and discuss the recent pandemic caused by the novel swine-origin influenza virus A/H1N1 with a focus on the mechanism of pathogenesis to obtain an insight into potential therapeutic strategies.
基金funded by the National Natural Science Foundation of China (32070678)the National Key Research and Development Program of China (2021YFC2302001).
文摘The Influenza A(H1N1)pdm09 virus caused a global pandemic in 2009 and has circulated seasonally ever since.As the continual genetic evolution of hemagglutinin in this virus leads to antigenic drift,rapid identification of antigenic variants and characterization of the antigenic evolution are needed.In this study,we developed PREDAC-H1pdm,a model to predict antigenic relationships between H1N1pdm viruses and identify antigenic clusters for post-2009 pandemic H1N1 strains.Our model performed well in predicting antigenic variants,which was helpful in influenza surveillance.By mapping the antigenic clusters for H1N1pdm,we found that substitutions on the Sa epitope were common for H1N1pdm,whereas for the former seasonal H1N1,substitutions on the Sb epitope were more common in antigenic evolution.Additionally,the localized epidemic pattern of H1N1pdm was more obvious than that of the former seasonal H1N1,which could make vaccine recommendation more sophisticated.Overall,the antigenic relationship prediction model we developed provides a rapid determination method for identifying antigenic variants,and the further analysis of evolutionary and epidemic characteristics can facilitate vaccine recommendations and influenza surveillance for H1N1pdm.
