The dependence of import of chicken heart apocytochrome c on its transformation to holoform by heme attachment was studied. Results showed that there was no difference in the translocation of apocytochrome c across th...The dependence of import of chicken heart apocytochrome c on its transformation to holoform by heme attachment was studied. Results showed that there was no difference in the translocation of apocytochrome c across the mitochondrial membrane in the presence or absence of hemin + dithionite. Furthermore, two heme unattached mutants (H18D, C17S) were prepared, which could still be accumulated in mitochondria, but their import velocity was obviously reduced.展开更多
基金Project supported by the National Natural Science Foundation of China (Grant No. 39730130).
文摘The dependence of import of chicken heart apocytochrome c on its transformation to holoform by heme attachment was studied. Results showed that there was no difference in the translocation of apocytochrome c across the mitochondrial membrane in the presence or absence of hemin + dithionite. Furthermore, two heme unattached mutants (H18D, C17S) were prepared, which could still be accumulated in mitochondria, but their import velocity was obviously reduced.
