Recently, ling et al. （2013） published the paper entitled as above. In Section 2.3, Adsorption kinetics of MBT, authors noticed that ＂The pseudo first-order kinetic model （Eq. （1）） and the pseudo second-order k...Recently, ling et al. （2013） published the paper entitled as above. In Section 2.3, Adsorption kinetics of MBT, authors noticed that ＂The pseudo first-order kinetic model （Eq. （1）） and the pseudo second-order kinetic model （Eq. （2））＂ were used （Do,an et al., 2007; Eftekhari et al., 2010）：展开更多
[目的]探讨红景天[Rhodiola crenulata(Hook.f.et.Thoms.)H.Ohba]提取物对小鼠免疫功能的影响。[方法]将192只BALB/c小鼠随机分为4批,每批分为4组,分别为低、中、高3个剂量组和1个溶剂对照组,连续灌药(20 m l/kg.体重)30 d,测定各免疫...[目的]探讨红景天[Rhodiola crenulata(Hook.f.et.Thoms.)H.Ohba]提取物对小鼠免疫功能的影响。[方法]将192只BALB/c小鼠随机分为4批,每批分为4组,分别为低、中、高3个剂量组和1个溶剂对照组,连续灌药(20 m l/kg.体重)30 d,测定各免疫指标。[结果]高剂量组红景天提取物可提高小鼠碳廓清能力(P<0.05);中剂量和高剂量组能增强绵羊红细胞诱导小鼠DTH能力(P<0.05),促进NK细胞活性(P<0.05)和血清凝血素的生成(P<0.05),增加抗体生成细胞数的生成(P<0.05),并且能提高小鼠腹腔巨噬细胞吞噬鸡红细胞的能力(P<0.05),但对免疫器官/体重质量值和ConA诱导的小鼠脾淋巴细胞转化能力无显著影响。[结论]红景天提取物具有增强正常小鼠免疫力的功能,该研究为开发有食用价值的保健食品提供了试验依据。展开更多
Objective To study the chemical constituents from the seeds of Ziziphus jujuba var.spinosa,which is used as a sedative herbal medicine in China.Methods The constituents were separated and purified by silica gel column...Objective To study the chemical constituents from the seeds of Ziziphus jujuba var.spinosa,which is used as a sedative herbal medicine in China.Methods The constituents were separated and purified by silica gel column.Their structures were elucidated based on their physicochemical properties and spectral analysis.Results Six compounds were obtained from 95%EtOH extracts in the seeds of Z.jujuba var.spinosa.They were identified as 2α,3β-dihydroxy-lup-20(29)-en-28-oic acid methyl ester(I),β-sitosterol(II),betulin(Ⅲ),betulinic acid(Ⅳ),hexadexanoic acid 2,3-dihydroxypropyl ester(V),and daucosterol(Ⅵ).Conclusion Compound I is a novel lupane triterpene named as alphitolicacid methyl ester,compound V is obtained from the seeds of Z.jujuba var.spinosa for the first time.展开更多
为开发新型天然烟用香料,采用乙醇加热回流酸枣[Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chow.]果肉制备浸膏,同时蒸馏萃取法提取浸膏中的挥发性成分,GC/MS法进行分离鉴定,并应用于卷烟加香。结果表明,1乙醇加热回流制备酸...为开发新型天然烟用香料,采用乙醇加热回流酸枣[Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chow.]果肉制备浸膏,同时蒸馏萃取法提取浸膏中的挥发性成分,GC/MS法进行分离鉴定,并应用于卷烟加香。结果表明,1乙醇加热回流制备酸枣浸膏得率为2.80%;2从酸枣浸膏中鉴定出69种挥发性成分,主要为有机酸、醇类、醛酮类、酯类等。其中,含量较高的成分主要有糠醛(2.88%)、月桂酸(13.00%)、肉豆蔻酸(6.40%)、十六碳烯醇(11.45%)、棕榈酸(10.54%)、反式-9-十六烯酸(反油酸)(28.16%)、顺-6-十八碳烯酸(2.89%)和亚油酸(1.36%)等;3将该浸膏用于卷烟加香能够明显使烟香丰满,刺激性减小,吸味醇和并能改善余味。展开更多
Objective:Rhodiolae Crenulatae Radix et Rhizoma(Hongjingtian in Chinese,RCRR),the roots and rhizomes of Rhodiola crenulata and its application in the medicinal market is very chaotic.In this study,DNA barcoding databa...Objective:Rhodiolae Crenulatae Radix et Rhizoma(Hongjingtian in Chinese,RCRR),the roots and rhizomes of Rhodiola crenulata and its application in the medicinal market is very chaotic.In this study,DNA barcoding database and identification engine of Rhodiola species were established,decoction pieces from the medicinal market were identified,and the application and challenges of DNA barcoding in the rapid radiation of Rhodiola species were analyzed.This study provides reference for the protection,rational development,and utilization of endangered resources within Rhodiola species.Methods:A total of 50 original plant samples from 20 species of the genus Rhodiola from Hebei,Xinjiang,Xizang,Jilin,and other major production areas were collected.Theses samples cover the typical distribution area(Qinghai-Tibetan Platea)of Rhodiola species and other scattered alpine regions(Changbai Mountain,Taibai Mountain,Lushan Mountain,etc.),it encompasses all Rhodiola species with thick rhizomes in China.ITS2 and psb A-trnH barcode of Rhodiola database(BORD)were established and an identification engine named Rhodiola-IDE was developed.The stability and accuracy of the standard DNA barcoding database were evaluated using two datasets.Rhodiola-IDE identified 31 decoction pieces of RCRR from the medicinal material market.Results:The BORD containing 1532 sequences of 88 Rhodiola species has been established,and the identification efficiency results showed good accuracy and stability.According to the Chinese Pharmacopoeia(2020 edition),23 samples(74.2%)were identified as authentic R.crenulata,while the rest of the marketed varieties were R.kirilowii,R.dumulosa,and R.fastigiata.The product label"Larger flower,Hongjingtian"was identified as R.crenulata.Samples labeled as"Smaller flower,Hongjingtian"were identified as R.crenulata,R.kirilowii,and R.fastigiata.Conclusion:ITS2 and psb A-trn H barcodes can identify monophyletic groups represented by R.crenulata.However,for non-monophyletic species,it is necessary to collect as many samples as possible and combine them with multiple markers for joint identification.This study discussed the application and challenges of DNA barcodes in Rhodiola under rapid radiation conditions,providing a scientific basis for the rational development and utilization of Rhodiola varieties.展开更多
Objective:To study bioactive compounds from the endophytic fungus Penicillium sp.HJT-A-6 isolated from stem of Rhodiola tibetica,and evaluate its allelopathic activity.Methods:The chemical constituents were isolated a...Objective:To study bioactive compounds from the endophytic fungus Penicillium sp.HJT-A-6 isolated from stem of Rhodiola tibetica,and evaluate its allelopathic activity.Methods:The chemical constituents were isolated and purified by silica gel,Sephadex LH-20 column chromatography and semi-preparative HPLC.Their structures were elucidated by extensive spectroscopic analysis and electronic circular dichroism(ECD)calculations.In addition,the allelopathic activity of compound 1 was evaluated by measuring the seed germination rate of R.tibetica.Results:Two new polyketides 4-hydroxy-3,6-dimethyl-2H-pyran-2-one(1)and penilactone E(2),together with six known compounds walterolactone A(3),5-hydroxyhexan-4-olide(4),3-methyl-2-penten-5-olide(5),chaetoquadrin F(6),(Z)-6-acetyl-3-(1,2-dihydroxypropylidene)-5-hydroxy-8-methyl chroman-2-one(7)and 4-hydroxy-3-(4-hydroxyhexanoyl)-5-methylfuran-2(5H)-one(8)were isolated from Penicillium sp.HJT-A-6.Compound 1 showed moderate seed-germination-promoting activity at a concentration of 0.001 mg/mL while inhibiting the seed germination at concentrations of 0.1 and0.01 mg/mL.Compared with the positive drug 6-benzyladenine(6-BA),compound 1 could extend the seed-germination period of R.tibetica(up to 11 d).Conclusion:Two new compounds were isolated from R.tibetica endophytic fungus Penicillium sp.HJT-A-6.Compound 1 displayed plant hormone-like activity,which inhibited the seed germination of the host plant at high concentrations and promoted the seed germination of the host plant at low concentrations.The results not only enrich the chemical constituents of the endophytic fungi isolated from Rhodiola tibetica,but also provide a theoretical basis for understanding the interaction mechanism between Rhodiola tibetica endophytic fungi and the host plant.展开更多
文摘Recently, ling et al. （2013） published the paper entitled as above. In Section 2.3, Adsorption kinetics of MBT, authors noticed that ＂The pseudo first-order kinetic model （Eq. （1）） and the pseudo second-order kinetic model （Eq. （2））＂ were used （Do,an et al., 2007; Eftekhari et al., 2010）：
文摘[目的]探讨红景天[Rhodiola crenulata(Hook.f.et.Thoms.)H.Ohba]提取物对小鼠免疫功能的影响。[方法]将192只BALB/c小鼠随机分为4批,每批分为4组,分别为低、中、高3个剂量组和1个溶剂对照组,连续灌药(20 m l/kg.体重)30 d,测定各免疫指标。[结果]高剂量组红景天提取物可提高小鼠碳廓清能力(P<0.05);中剂量和高剂量组能增强绵羊红细胞诱导小鼠DTH能力(P<0.05),促进NK细胞活性(P<0.05)和血清凝血素的生成(P<0.05),增加抗体生成细胞数的生成(P<0.05),并且能提高小鼠腹腔巨噬细胞吞噬鸡红细胞的能力(P<0.05),但对免疫器官/体重质量值和ConA诱导的小鼠脾淋巴细胞转化能力无显著影响。[结论]红景天提取物具有增强正常小鼠免疫力的功能,该研究为开发有食用价值的保健食品提供了试验依据。
文摘Objective To study the chemical constituents from the seeds of Ziziphus jujuba var.spinosa,which is used as a sedative herbal medicine in China.Methods The constituents were separated and purified by silica gel column.Their structures were elucidated based on their physicochemical properties and spectral analysis.Results Six compounds were obtained from 95%EtOH extracts in the seeds of Z.jujuba var.spinosa.They were identified as 2α,3β-dihydroxy-lup-20(29)-en-28-oic acid methyl ester(I),β-sitosterol(II),betulin(Ⅲ),betulinic acid(Ⅳ),hexadexanoic acid 2,3-dihydroxypropyl ester(V),and daucosterol(Ⅵ).Conclusion Compound I is a novel lupane triterpene named as alphitolicacid methyl ester,compound V is obtained from the seeds of Z.jujuba var.spinosa for the first time.
文摘为开发新型天然烟用香料,采用乙醇加热回流酸枣[Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chow.]果肉制备浸膏,同时蒸馏萃取法提取浸膏中的挥发性成分,GC/MS法进行分离鉴定,并应用于卷烟加香。结果表明,1乙醇加热回流制备酸枣浸膏得率为2.80%;2从酸枣浸膏中鉴定出69种挥发性成分,主要为有机酸、醇类、醛酮类、酯类等。其中,含量较高的成分主要有糠醛(2.88%)、月桂酸(13.00%)、肉豆蔻酸(6.40%)、十六碳烯醇(11.45%)、棕榈酸(10.54%)、反式-9-十六烯酸(反油酸)(28.16%)、顺-6-十八碳烯酸(2.89%)和亚油酸(1.36%)等;3将该浸膏用于卷烟加香能够明显使烟香丰满,刺激性减小,吸味醇和并能改善余味。
基金supported by China Postdoctoral Science Foundation(No.2022M720504)the National Key Research and Development Program of China:Intergovernmental Cooperation in International Science and Technology Innovation(No.2022YFE0119300)+3 种基金CAMS Innovation Fund for Medical Sciences(CIFMS)(No.2021-I2M-1-029 and No.2021-I2M-1-032)Open project supported by Hebei(Chengde)Industrial Technology Institute of Chinese Medicinal Materials(No.CYKF202301)the graduate student innovation ability training of Education of Hebei Province Department(No.CXZZSS2024118)Key project at central government level:The ability establishment of sustainable use for valuable Chinese medicine resources(No.2060302)。
文摘Objective:Rhodiolae Crenulatae Radix et Rhizoma(Hongjingtian in Chinese,RCRR),the roots and rhizomes of Rhodiola crenulata and its application in the medicinal market is very chaotic.In this study,DNA barcoding database and identification engine of Rhodiola species were established,decoction pieces from the medicinal market were identified,and the application and challenges of DNA barcoding in the rapid radiation of Rhodiola species were analyzed.This study provides reference for the protection,rational development,and utilization of endangered resources within Rhodiola species.Methods:A total of 50 original plant samples from 20 species of the genus Rhodiola from Hebei,Xinjiang,Xizang,Jilin,and other major production areas were collected.Theses samples cover the typical distribution area(Qinghai-Tibetan Platea)of Rhodiola species and other scattered alpine regions(Changbai Mountain,Taibai Mountain,Lushan Mountain,etc.),it encompasses all Rhodiola species with thick rhizomes in China.ITS2 and psb A-trnH barcode of Rhodiola database(BORD)were established and an identification engine named Rhodiola-IDE was developed.The stability and accuracy of the standard DNA barcoding database were evaluated using two datasets.Rhodiola-IDE identified 31 decoction pieces of RCRR from the medicinal material market.Results:The BORD containing 1532 sequences of 88 Rhodiola species has been established,and the identification efficiency results showed good accuracy and stability.According to the Chinese Pharmacopoeia(2020 edition),23 samples(74.2%)were identified as authentic R.crenulata,while the rest of the marketed varieties were R.kirilowii,R.dumulosa,and R.fastigiata.The product label"Larger flower,Hongjingtian"was identified as R.crenulata.Samples labeled as"Smaller flower,Hongjingtian"were identified as R.crenulata,R.kirilowii,and R.fastigiata.Conclusion:ITS2 and psb A-trn H barcodes can identify monophyletic groups represented by R.crenulata.However,for non-monophyletic species,it is necessary to collect as many samples as possible and combine them with multiple markers for joint identification.This study discussed the application and challenges of DNA barcodes in Rhodiola under rapid radiation conditions,providing a scientific basis for the rational development and utilization of Rhodiola varieties.
基金supported by Central Government Guided Local Science and Technology Development Fund Project(No.XZ202101YD0017C)International Science and Technology Cooperation Project of Dalian Science and Technology Innovation Fund(No.2022JJ12WZ059)。
文摘Objective:To study bioactive compounds from the endophytic fungus Penicillium sp.HJT-A-6 isolated from stem of Rhodiola tibetica,and evaluate its allelopathic activity.Methods:The chemical constituents were isolated and purified by silica gel,Sephadex LH-20 column chromatography and semi-preparative HPLC.Their structures were elucidated by extensive spectroscopic analysis and electronic circular dichroism(ECD)calculations.In addition,the allelopathic activity of compound 1 was evaluated by measuring the seed germination rate of R.tibetica.Results:Two new polyketides 4-hydroxy-3,6-dimethyl-2H-pyran-2-one(1)and penilactone E(2),together with six known compounds walterolactone A(3),5-hydroxyhexan-4-olide(4),3-methyl-2-penten-5-olide(5),chaetoquadrin F(6),(Z)-6-acetyl-3-(1,2-dihydroxypropylidene)-5-hydroxy-8-methyl chroman-2-one(7)and 4-hydroxy-3-(4-hydroxyhexanoyl)-5-methylfuran-2(5H)-one(8)were isolated from Penicillium sp.HJT-A-6.Compound 1 showed moderate seed-germination-promoting activity at a concentration of 0.001 mg/mL while inhibiting the seed germination at concentrations of 0.1 and0.01 mg/mL.Compared with the positive drug 6-benzyladenine(6-BA),compound 1 could extend the seed-germination period of R.tibetica(up to 11 d).Conclusion:Two new compounds were isolated from R.tibetica endophytic fungus Penicillium sp.HJT-A-6.Compound 1 displayed plant hormone-like activity,which inhibited the seed germination of the host plant at high concentrations and promoted the seed germination of the host plant at low concentrations.The results not only enrich the chemical constituents of the endophytic fungi isolated from Rhodiola tibetica,but also provide a theoretical basis for understanding the interaction mechanism between Rhodiola tibetica endophytic fungi and the host plant.
