Gynogenesis is an established technique to generate all female type offspring and this technique has been successfully induced diploid gynogens progeny in aquatic animals of fishes and crustaceans.Monosex culture of a...Gynogenesis is an established technique to generate all female type offspring and this technique has been successfully induced diploid gynogens progeny in aquatic animals of fishes and crustaceans.Monosex culture of all female shrimp and fishes were selected attribute to all female type offspring which have better size than male and help increase the market size and profitable.This article discusses on the protocol applied to produce gynogens progeny and the successful rate of gynogenesis production in fishes,molluscs and aquatic crustaceans of shrimps in general.Overall most of the UV length applied for irradiated the sperm were around 254-365 nm for(20-40 s),(20-80 s)and(5-8 s)for shrimps,254 nm,30 s for molluscs species and for fishes were around 254 nm for(1.5min)and(2-12 min)time of exposure respectively.For gynogenesis induction,the fertilized eggs were treated with cold shock,heat shock or cytochalasin-B for both shrimp and fishes gynogens technique.Fertilization rate was identified around 4.33%-19.67%in shrimp.Successful hatching rate was identified around 3.0%,14.9%-37.2%of gynogens offspring in shrimp and various percentages of hatching rates were identified from each species of fish gynogens.Overall,there is still low survival rate of gynogens produced using gynogenesis technique and further study should be carried out to improve the gynogens production.The discussed protocols serve as a guide lines for the gynogenesis technique application of all female monosex culture in the future.展开更多
Diploid(2n)gynogens,triploid(3n)hybrids and 2n hybrids fish were produced in meiotic gynogenetic offsprings of grass carp(Ctenopharyngodon idella)eggs using UV-irradiated blunt snout bream(Megalobrama amblycephala)spe...Diploid(2n)gynogens,triploid(3n)hybrids and 2n hybrids fish were produced in meiotic gynogenetic offsprings of grass carp(Ctenopharyngodon idella)eggs using UV-irradiated blunt snout bream(Megalobrama amblycephala)sperm.Flow cytometry revealed that a total of 306(54.2%)2n gynogens,157(27.7%)2n hybrids and 102(18.1%)3n hybrids were identified at one year old stage examined.Chromosome analysis showed that 2n gynogens and 2n hybrid individuals had the same chromosome number as their parents(2n¼48),while the chromosome number of 3n hybrids was 3n¼72.The morphological characters of 2n hybrids and 3n hybrids were intermediate between their parents,and the 2n gynogens were similar to grass carp.Microsatellite genetic analysis showed that the expected heterozygosity(He)of 2n gynogens,2n hybrids,3n hybrids and grass carp control were 0.3775,0.6518,0.6601,0.6502,respectively,and the polymorphism information content(PIC)was 0.3791,0.5749,0.5907,0.5738,respectively.Compared with 2n and 3n hybrids,the He and PIC of 2n gynogens were significantly decreased,indicating that high homozygous could be obtained by the gynogenetic method.The 3n hybrids grew faster than both 2n gynogens and 2n hybrid individuals,suggesting that 3n hybrids had an obvious growth advantage.展开更多
文摘Gynogenesis is an established technique to generate all female type offspring and this technique has been successfully induced diploid gynogens progeny in aquatic animals of fishes and crustaceans.Monosex culture of all female shrimp and fishes were selected attribute to all female type offspring which have better size than male and help increase the market size and profitable.This article discusses on the protocol applied to produce gynogens progeny and the successful rate of gynogenesis production in fishes,molluscs and aquatic crustaceans of shrimps in general.Overall most of the UV length applied for irradiated the sperm were around 254-365 nm for(20-40 s),(20-80 s)and(5-8 s)for shrimps,254 nm,30 s for molluscs species and for fishes were around 254 nm for(1.5min)and(2-12 min)time of exposure respectively.For gynogenesis induction,the fertilized eggs were treated with cold shock,heat shock or cytochalasin-B for both shrimp and fishes gynogens technique.Fertilization rate was identified around 4.33%-19.67%in shrimp.Successful hatching rate was identified around 3.0%,14.9%-37.2%of gynogens offspring in shrimp and various percentages of hatching rates were identified from each species of fish gynogens.Overall,there is still low survival rate of gynogens produced using gynogenesis technique and further study should be carried out to improve the gynogens production.The discussed protocols serve as a guide lines for the gynogenesis technique application of all female monosex culture in the future.
基金This research was supported by grants from the National Science Foundation of China(3127263331201760)+1 种基金the National High Technology Research and Development Program of China(863 Program)(2011AA100403)the Project of Shanghai Educational Committee(ZF1206).
文摘Diploid(2n)gynogens,triploid(3n)hybrids and 2n hybrids fish were produced in meiotic gynogenetic offsprings of grass carp(Ctenopharyngodon idella)eggs using UV-irradiated blunt snout bream(Megalobrama amblycephala)sperm.Flow cytometry revealed that a total of 306(54.2%)2n gynogens,157(27.7%)2n hybrids and 102(18.1%)3n hybrids were identified at one year old stage examined.Chromosome analysis showed that 2n gynogens and 2n hybrid individuals had the same chromosome number as their parents(2n¼48),while the chromosome number of 3n hybrids was 3n¼72.The morphological characters of 2n hybrids and 3n hybrids were intermediate between their parents,and the 2n gynogens were similar to grass carp.Microsatellite genetic analysis showed that the expected heterozygosity(He)of 2n gynogens,2n hybrids,3n hybrids and grass carp control were 0.3775,0.6518,0.6601,0.6502,respectively,and the polymorphism information content(PIC)was 0.3791,0.5749,0.5907,0.5738,respectively.Compared with 2n and 3n hybrids,the He and PIC of 2n gynogens were significantly decreased,indicating that high homozygous could be obtained by the gynogenetic method.The 3n hybrids grew faster than both 2n gynogens and 2n hybrid individuals,suggesting that 3n hybrids had an obvious growth advantage.
