Guluronate oligosaccharides(GOS)is a biologically active natural product derived from brown algae.This study aimed to evaluate the amelioration of hyperuricemia and renal inflammation by GOS and its mechanisms.Accordi...Guluronate oligosaccharides(GOS)is a biologically active natural product derived from brown algae.This study aimed to evaluate the amelioration of hyperuricemia and renal inflammation by GOS and its mechanisms.According to the results,GOS treatment reduced the serum uric acid(UA)and blood urea nitrogen(BUN)levels among hyperuricemic mice by 19.6%and 21.5%(P<0.05),at the same time,serum xanthine oxidase(XOD)and adenosine deaminase(ADA)levels were down-regulated to normal.GOS treatment ameliorated the renal pathologization associated with hyperuricemia and reduced renal IL-1β,IL-12,and IL-18(P<0.05),suggesting a mitigating effect of GOS on renal damage.In addition,GOS supplementation modulates the mRNA levels of renal and intestinal urate transporters to promote uric acid excretion.GOS reversed the intestinal microbiota of hyperuricemic mice,significantly down-regulating the abundances of pernicious bacteria like Bilophila and Tuzzerella,while up-regulating the beneficial intestinal bacteria including Muribaculum,Lachnospiraceae_UCG_006,Ruminococcus,Faecalibaculum,and Lachnospiraceae_NK4A136_group.Together,guluronate oligosaccharides can improve hyperuricemia by alleviating hyperuricemia-induced renal damage and reversing intestinal microbiota disorders.These findings provide evidence supporting the further development of guluronate oligosaccharides as a novel functional food ingredient suitable for hyperuricemia.展开更多
Under certain conditions algal alginates bind pinacyanol chloride in the form of dimers – this is the conclusion drawn from a uv/vis and circular dichroism (CD) spectroscopic study of aqueous solutions of the dye in ...Under certain conditions algal alginates bind pinacyanol chloride in the form of dimers – this is the conclusion drawn from a uv/vis and circular dichroism (CD) spectroscopic study of aqueous solutions of the dye in a 10-fold molar excess of the polysaccharide from different sources. With its easily detected features the dimer holds promise as a diagnostic tool for alginate conformational analysis. Binding of the strongly blue-shifted (maximum wavelength = 485 nm) dimer is probably mediated by the charged groups and involves guluronate units only. By using the peakFit program, the two overlapping excitonic absorption bands together with the optically inactive band resulted from the interaction of pinacyanol with a specific alginate concentration were separated. The standard Gibbs energy of the interaction was calculated as –27.02 kJ.mol–1. The dimers are sensitive against acid and divalent cations.展开更多
基金supported by the National Key Research and Development Program(2019YFC1604605).
文摘Guluronate oligosaccharides(GOS)is a biologically active natural product derived from brown algae.This study aimed to evaluate the amelioration of hyperuricemia and renal inflammation by GOS and its mechanisms.According to the results,GOS treatment reduced the serum uric acid(UA)and blood urea nitrogen(BUN)levels among hyperuricemic mice by 19.6%and 21.5%(P<0.05),at the same time,serum xanthine oxidase(XOD)and adenosine deaminase(ADA)levels were down-regulated to normal.GOS treatment ameliorated the renal pathologization associated with hyperuricemia and reduced renal IL-1β,IL-12,and IL-18(P<0.05),suggesting a mitigating effect of GOS on renal damage.In addition,GOS supplementation modulates the mRNA levels of renal and intestinal urate transporters to promote uric acid excretion.GOS reversed the intestinal microbiota of hyperuricemic mice,significantly down-regulating the abundances of pernicious bacteria like Bilophila and Tuzzerella,while up-regulating the beneficial intestinal bacteria including Muribaculum,Lachnospiraceae_UCG_006,Ruminococcus,Faecalibaculum,and Lachnospiraceae_NK4A136_group.Together,guluronate oligosaccharides can improve hyperuricemia by alleviating hyperuricemia-induced renal damage and reversing intestinal microbiota disorders.These findings provide evidence supporting the further development of guluronate oligosaccharides as a novel functional food ingredient suitable for hyperuricemia.
文摘Under certain conditions algal alginates bind pinacyanol chloride in the form of dimers – this is the conclusion drawn from a uv/vis and circular dichroism (CD) spectroscopic study of aqueous solutions of the dye in a 10-fold molar excess of the polysaccharide from different sources. With its easily detected features the dimer holds promise as a diagnostic tool for alginate conformational analysis. Binding of the strongly blue-shifted (maximum wavelength = 485 nm) dimer is probably mediated by the charged groups and involves guluronate units only. By using the peakFit program, the two overlapping excitonic absorption bands together with the optically inactive band resulted from the interaction of pinacyanol with a specific alginate concentration were separated. The standard Gibbs energy of the interaction was calculated as –27.02 kJ.mol–1. The dimers are sensitive against acid and divalent cations.
