Polycystic ovary syndrome(PCOS)is a prevalent endocrine disorder that poses a significant threat to women’s health.As a classical traditional Chinese medicine(TCM)formulation,Guizhi-Fuling Wan(GFW)has a good applicat...Polycystic ovary syndrome(PCOS)is a prevalent endocrine disorder that poses a significant threat to women’s health.As a classical traditional Chinese medicine(TCM)formulation,Guizhi-Fuling Wan(GFW)has a good application prospect in the complementary treatment of PCOS.This study aimed to systematically summarize the traditional efficacy and pharmacological composition of the Chinese medicines contained in GFW and evaluate the clinical efficacy and safety of their active ingredients in the complementary treatment of PCOS.A growing number of studies have demonstrated that GFW is effective at complementally treating PCOS through various mechanisms,including inhibiting inflammatory responses,modulating the intraovarian extracellular matrix,regulating apoptosis and autophagy in granulosa cells,and correcting oxidative stress imbalances.In addition,the GFW has been shown to be effective in treating the complications of PCOS.However,there are several problems,and future work should focus on elucidating the efficacy,safe dosage,and safety of different dosage forms of GFW,increasing the toxicological value of traditional Chinese medicine,and confirming the safety of the combination of GFW with Western medicine for the treatment of PCOS.展开更多
Objective:Ovarian cancer(OC)is the most lethal gynecological malignancy.Frequent peritoneal dissemination is the main cause of low survival rate.Guizhi-Fuling formula(GZFL)is a classical traditional Chinese herbal for...Objective:Ovarian cancer(OC)is the most lethal gynecological malignancy.Frequent peritoneal dissemination is the main cause of low survival rate.Guizhi-Fuling formula(GZFL)is a classical traditional Chinese herbal formula,and has been clinically used for treating ovarian cancer with good outcome.However,its therapeutic mechanism for treating OC has not been clearly elucidated.Methods:Network pharmacology analysis was used to predict potential molecular mechanisms of GZFL in treating OC.In vitro and in vivo analysis,including STAT3 KO/WT cells proliferation assay,scratch assay and antitumor efficacy study were performed to assess the biological activity of GZFL on targeting STAT3 in OC cells.Results:We generated a“GZFL target-OC-STAT3”gene interaction network,and predicted that GZFL is tightly associated with IL6/JAK/STAT3 signal pathway and cancer metastasis.Our preliminary data showed that GZFL inhibited OC cell proliferation in a STAT3 dependent manner.It suppressed cell migration and downregulated p-STAT3 expression.In a tumor bearing mouse model,GZFL displayed a safety profile.Conclusion:GZFL inhibits OC progression by targeting STAT3 signaling network.Our newly proposed pharmacological mechanisms of Guizhi-Fuling formula will provide a new insight for its clinical use in treating OC.展开更多
基金supported by funds from the Natural Science Foundation of Shandong Province(No.ZR2020MH346No.ZR2021MH003)the National Natural Science Foundation of China(No.81273667).
文摘Polycystic ovary syndrome(PCOS)is a prevalent endocrine disorder that poses a significant threat to women’s health.As a classical traditional Chinese medicine(TCM)formulation,Guizhi-Fuling Wan(GFW)has a good application prospect in the complementary treatment of PCOS.This study aimed to systematically summarize the traditional efficacy and pharmacological composition of the Chinese medicines contained in GFW and evaluate the clinical efficacy and safety of their active ingredients in the complementary treatment of PCOS.A growing number of studies have demonstrated that GFW is effective at complementally treating PCOS through various mechanisms,including inhibiting inflammatory responses,modulating the intraovarian extracellular matrix,regulating apoptosis and autophagy in granulosa cells,and correcting oxidative stress imbalances.In addition,the GFW has been shown to be effective in treating the complications of PCOS.However,there are several problems,and future work should focus on elucidating the efficacy,safe dosage,and safety of different dosage forms of GFW,increasing the toxicological value of traditional Chinese medicine,and confirming the safety of the combination of GFW with Western medicine for the treatment of PCOS.
文摘Objective:Ovarian cancer(OC)is the most lethal gynecological malignancy.Frequent peritoneal dissemination is the main cause of low survival rate.Guizhi-Fuling formula(GZFL)is a classical traditional Chinese herbal formula,and has been clinically used for treating ovarian cancer with good outcome.However,its therapeutic mechanism for treating OC has not been clearly elucidated.Methods:Network pharmacology analysis was used to predict potential molecular mechanisms of GZFL in treating OC.In vitro and in vivo analysis,including STAT3 KO/WT cells proliferation assay,scratch assay and antitumor efficacy study were performed to assess the biological activity of GZFL on targeting STAT3 in OC cells.Results:We generated a“GZFL target-OC-STAT3”gene interaction network,and predicted that GZFL is tightly associated with IL6/JAK/STAT3 signal pathway and cancer metastasis.Our preliminary data showed that GZFL inhibited OC cell proliferation in a STAT3 dependent manner.It suppressed cell migration and downregulated p-STAT3 expression.In a tumor bearing mouse model,GZFL displayed a safety profile.Conclusion:GZFL inhibits OC progression by targeting STAT3 signaling network.Our newly proposed pharmacological mechanisms of Guizhi-Fuling formula will provide a new insight for its clinical use in treating OC.
