Agrochemicals,especially plant growth regulators(PGRs),are extensively used to modulate endogenous phytohormone signals in small quantities,significantly infiuencing plant growth and development.Plant hormones typical...Agrochemicals,especially plant growth regulators(PGRs),are extensively used to modulate endogenous phytohormone signals in small quantities,significantly infiuencing plant growth and development.Plant hormones typically exhibit diverse chemical structures,with common examples including indole rings,terpenoid frameworks,adenine motifs,cyclic lactones,cyclopentanones,and steroidal compounds,which are extensively employed in pesticides.This article explores the interactions and biological activities of small molecules on proteins,enzymes,and other reactive sites involved in the biosynthesis,metabolism,transport,and signal transduction pathways of various plant hormones.Additionally,it analyzes the structure-activity relationships(SARs)of pesticides incorporating these structural motifs to elucidate the relationship between active fragments,pharmacophores,and targets,highlighting the characteristics of potent small molecules and their derivatives.This comprehensive review aims to provide novel perspectives for the development and design of pesticides,offering valuable insights for researchers in the field.展开更多
[Objective] This study aimed to investigate the influence of different plant growth regulators on apricot pollen germination and pollen tube growth. [Method] Pollens of six kinds of Xinjiang apricots were cultured in ...[Objective] This study aimed to investigate the influence of different plant growth regulators on apricot pollen germination and pollen tube growth. [Method] Pollens of six kinds of Xinjiang apricots were cultured in solid media supplemented with five plant growth regulators （GA3 , NAA, 2, 4-D, 6-BA, IAA）. Then the rate of pollen germination and the length of pollen tube were respectively measured. [Result] In a certain concentration range, GA3 most significantly promoted the pollen germination and the pollen tube growth of Shushanggan, Kalayulvke, Dayoujia, Yiliakeyulvke and Kabakehuanna; NNA had the strongest improvement function on Kumaiti’s pollen germination and pollen tube growth. [Conclusion] All the five plant growth regulators promoted the pollen germination and the pollen tube growth of apricots at low concentration but inhibited them at high concentration.展开更多
The effects of four kinds of plant growth regulators with different concentrations on narcissi were studied in 2001. The results showed that the regulators could inhibit the growths of height and leaves of narcissi. O...The effects of four kinds of plant growth regulators with different concentrations on narcissi were studied in 2001. The results showed that the regulators could inhibit the growths of height and leaves of narcissi. Of the four regulators, the dwarfing effects of paclobatrazol (PP333) and uniconazole (S3307) on narcissi were better than those of chlorocholine (CCC) and dimethyl amino-sussinamic acid (B9). All of the regulators did not have significant effect on the root length. Moreover, the time of flowering was later for the narcissi treated with regulators than that of the control to a certain extent, and the range delayed was from 2 days to 19 days. The correlation analysis results showed that there was a significant correlation between the time of flowering and the concentrations of regulators. The ornament value of narcissi was obviously improved by using the regulators.展开更多
Background Zonal application maps are designed to represent field variability using key variables that can be translated into tailored management practices.For cotton,zonal maps for crop growth regulator(CGR)applicati...Background Zonal application maps are designed to represent field variability using key variables that can be translated into tailored management practices.For cotton,zonal maps for crop growth regulator(CGR)applications under variable-rate(VR)strategies are commonly based exclusively on vegetation indices(VIs)variability.However,VIs often saturate in dense crop vegetation areas,limiting their effectiveness in distinguishing variability in crop growth.This study aimed to compare unsupervised framework(UF)and supervised framework(SUF)approaches for generat-ing zonal application maps for CGR under VR conditions.During 2022-2023 agricultural seasons,an UF was employed to generate zonal maps based on locally collected field data on plant height of cotton,satellite imagery,soil texture,and phenology data.Subsequently,a SUF(based on historical data between 2020-2021 to 2022-2023 agricultural seasons)was developed to predict plant height using remote sensing and phenology data,aiming to replicate same zonal maps but without relying on direct field measurements of plant height.Both approaches were tested in three fields and on two different dates per field.Results The predictive model for plant height of SUF performed well,as indicated by the model metrics.However,when comparing zonal application maps for specific field-date combinations,the predicted plant height exhibited lower variability compared with field measurements.This led to variable compatibility between SUF maps,which utilized the model predictions,and the UF maps,which were based on the real field data.Fields characterized by much pronounced soil texture variability yielded the highest compatibility between the zonal application maps produced by both SUF and UF approaches.This was predominantly due to the greater consistency in estimating plant development patterns within these heterogeneous field environments.While VR application approach can facilitate product savings during the application operation,other key factors must be considered.These include the availability of specialized machinery required for this type of applications,as well as the inherent operational costs associated with applying a single CGR product which differs from the typical uniform rate applications that often integrate multi-ple inputs.Conclusion Predictive modeling shows promise for assisting in the creation of zonal application maps for VR of CGR applications.However,the degree of agreement with the actual variability in crop growth found in the field should be evaluated on a field-by-field basis.The SUF approach,which is based on plant heigh prediction,demonstrated potential for supporting the development of zonal application maps for VR of CGR applications.However,the degree to which this approach aligns itself with the actual variability in crop growth observed in the field may vary,necessi-tating field-by-field evaluation.展开更多
This paper reviewed the toxicity,maximum residue limits(MRLs)and current residue status of commonly used plant growth regulators in vegetables,including 2,4-dichlorophenoxyacetic acid(2,4-D),naphthaleneacetic acid(NAA...This paper reviewed the toxicity,maximum residue limits(MRLs)and current residue status of commonly used plant growth regulators in vegetables,including 2,4-dichlorophenoxyacetic acid(2,4-D),naphthaleneacetic acid(NAA),ethephon,gibberellin,and paclobutrazol.Methods for reducing residues of plant growth regulators in vegetables were discussed,and recommendations and strategies for their application were proposed.展开更多
The effect of vernalization and two growth regulators Fascination^TM and Pro-Gibb^R on the growth, inflorescence development and flowering of Omithogalurrt Chesapeake Snowflake＇ was studied. Regardless of growth regu...The effect of vernalization and two growth regulators Fascination^TM and Pro-Gibb^R on the growth, inflorescence development and flowering of Omithogalurrt Chesapeake Snowflake＇ was studied. Regardless of growth regulator treatment, chilling bulbs for 3 weeks at 10℃ before planting accelerated flowering of the first inflorescence by 5 to 6 days, elongated floral stem length by 1.5 to 2 cm and shortened leaf length by 4 to 5 cm as compared with non-chilled bulbs. When bulbs were chilled 3 weeks at 10℃ before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 2 to 6 days respectively as compared with the control. When bulbs were not chilled before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 6 to12 days respectively as compared with the control. The lengths of the leaves and the inflorescences were not affected by PGR treatment. Higher rates of Pro-Gibb （100 mg.L^-1 and 200 mg.L^-1） and more amount of Fascination 2%（100 μL and 200 μL） can cause abortion of inflorescence.展开更多
The research reviewed use effects of Yuhuangjin, Xishibao, Zhuangfengling and Jianzhuangsu on Jinhai No. 5. The results showed plant height and ear height declined in varying degrees, as well as empty-stalk rate and l...The research reviewed use effects of Yuhuangjin, Xishibao, Zhuangfengling and Jianzhuangsu on Jinhai No. 5. The results showed plant height and ear height declined in varying degrees, as well as empty-stalk rate and lodging rate, with the plant growth regulators applied. Economic characters all improved, including ear length and diameter, barren-tip length and hundred-seed weight, and corn yield went up significantly on average. For example, the increased yield can be as high as 17.43% when Yuhuangjin was applied at 30 ml/hm^2.展开更多
[Objective]In order to increase anther culture efficiency of pepper.[Method]MS culture media and Bolajiaohong were used in this experiment to study the influences of carbon sources and concentrations on anther callus ...[Objective]In order to increase anther culture efficiency of pepper.[Method]MS culture media and Bolajiaohong were used in this experiment to study the influences of carbon sources and concentrations on anther callus induction of pepper.Jiayu was taken as a material to study influences of plant growth regulators and concentrations on anther callus induction of pepper according to L16（4^5） orthogonal design.[Result]The average callus and embryoid induction rates of maltose at all concentrations were higher than these of sucrose but the difference was not significant.Taking maltose or sucrose as a carbon source,3% to 6% concentration was good for increasing induction frequencies of calli and embryoids.However,If the concentration was over 6%,the induction rates were declined dramatically with the increase of sugar concentration.The influences of growth regulators on induction rate of calli were listed as 2,4-D﹥ZT﹥NAA﹥KT﹥6-BA;the influences on induction rates of embryoids were listed as 2,4-D﹥NAA﹥ZT﹥KT﹥6-BA.The 2,4-D,ZT,NAA and KT had signficant or extremely significant influences on induction rates of calli and embryoids.2,4-D,ZT at 1.0 mg/L and NNA,KT at 0.5 mg/L had the best effects.The influences of ZT on calli and embryoids were better than those of KT and 6-BA.1.0 mg/L 2,4-D ＋1.0 mg/L ZT ＋0.5 mg/L KT ＋0.5 mg/L 6-BA was the best regulator combination for induction culture of Jiayu anther.[Conclusion]The experiment provided research basis for anther culture of pepper.展开更多
The endangered tropical tree, Aquilaria malaccensis, produces agarwood for use in fragrance and medicines. Efforts are currently un-derway to produce valuable agarwood compoundsn tissue culture. The purpose of this st...The endangered tropical tree, Aquilaria malaccensis, produces agarwood for use in fragrance and medicines. Efforts are currently un-derway to produce valuable agarwood compoundsn tissue culture. The purpose of this study was to develop an optimal growth medium, specif-ically, the best hormone combination for callus suspension culture. Using nursery-grown A. malaccensis, sterilized leaf explants were first incu-bated on basic Murashige and Skoog （MS） gel medium containing 15g/L sucrose and at pH 5.7. Different auxin types including 1-naphthaleneacetic acid （NAA）, 2,4-dichlorophenoxyacetic acid （2,4-D）, and indole-3-butyric acid （IBA）, were tested at various concentrations （0.55, 1.1 and 1.65 μM） using the basic medium. Leaf explants were incubated for 30 days in the dark. Callus induced by 1.1 μM NAA had the highest biomass dry weight （DW） of 17.3 mg;however the callus was of a compact type. This auxin concentration was then combined with either 6-benzylaminopurine （BAP） or kinetin at 0.55, 1.1, 2.2 or 3.3 μM to induce growth of friable callus. The 1.1μM NAA＋2.2μM BAP com-bination produced friable callus with the highest biomass （93.3mg DW）. When testing the different carbon sources and pHs, sucrose at 15g/L and pH at 5.7 yielded highest biomasses at 87.7mg and 83mg DW, respec-tively. Microscopic observations revealed the arrangement of the friable cells as loosely packed with relatively large cells, while for the compact callus, the cells were small and densely packed. We concluded that MS medium containing 15 g/L sucrose, 1.1 μM NAA ＋ 2.2 μM BAP hor-mone combination, and a pH of 5.7 was highly effective for inducing friable callus from leaf explants of A. malaccensis for the purpose of establishing cell suspension culture.展开更多
Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medici...Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs.展开更多
Joint toxicity of Beauveria bassiana and two insect growth regulators, methoxyfenozide and hexaflumuron, on beet annywonn larvae, Spodoptera exigua （ Htthner）, were investigated. An obvious synergism effect of the t...Joint toxicity of Beauveria bassiana and two insect growth regulators, methoxyfenozide and hexaflumuron, on beet annywonn larvae, Spodoptera exigua （ Htthner）, were investigated. An obvious synergism effect of the two insect growth regulators with B. beauveria was observed. When the insect growth regulators at LC50 concentrations （25 × 10^-6 g/L for methoxyfenozide and 1 000 × 10^ -6 g/L for hexaflumuren） were mixed with B. bassiana at the volume ratio of 4： 1, the mixtures achieved the best efficacy. The co-toxicity co-efficient （CTC） of methexyfenozide and B. bass/ana was 285, and that of hexaflumuron and B. bass/ana was 208. Scanning electron microscope observation of the longitudinal section of S. exigua revealed obviously different patterns of integument in different treatment groups.展开更多
In this study,the seeds of wild Petunia Juss.were used as explants to investigate the optimal condition for tissue culture.Several different kinds and concentrations of growth regulators were adopted to produce more m...In this study,the seeds of wild Petunia Juss.were used as explants to investigate the optimal condition for tissue culture.Several different kinds and concentrations of growth regulators were adopted to produce more multiple bud clumps,callus or roots in this study.The experiments may provide experimental foundation for the rapid propagation technology and establishment of tissue culture system for wild Petunia Juss.展开更多
Prunus africana is an endangered medicinal species and has been classified as a priority for domestication in Cameroon.However,the seeds rapidly lose their viability during storage at room temperature after 2–3 month...Prunus africana is an endangered medicinal species and has been classified as a priority for domestication in Cameroon.However,the seeds rapidly lose their viability during storage at room temperature after 2–3 months.This study aimed to improve seed germination of P.africana by germinating at different temperatures(4℃,28℃)using different concentrations of growth stimulators(sodium nitrate and gibberellic acid)and different concentrations of salts.P.africana seeds had 91.7%germination at 4C after 1 month of storage.Growth regulators considerably influenced germination after 6 months and reached 66.0%with 10 mM gibberellic acid and 100%with 10 mM sodium nitrate.Approximately three shoots per seed were developed,an indication of polyembryony.Histochemical analyses revealed the presence of protein-like bodies close to the embryo axis and accumulations of starch after 7 days of germination.After 14 days,amyloplasts and dark protein bodies of various sizes were observed.The outcome of this study will contribute to improve the germination of P.africana for better domestication and conservation.展开更多
Insect growth regulators play an important role in integrated pest management strategies.The FGLa–allatostatins（ASTs）are a family of neuropeptides that can inhibit juvenile hormone（JH）biosynthesis by the corpora ...Insect growth regulators play an important role in integrated pest management strategies.The FGLa–allatostatins（ASTs）are a family of neuropeptides that can inhibit juvenile hormone（JH）biosynthesis by the corpora allata（CA）of Diploptera punctata in vitro,are regarded as insect growth regulator candidates.In the search for new potential mimics and to explore the effect of linker length on inhibiting JH biosynthesis,a series of AST analogs were synthesized by modifying the linker of K24,which was found to have a significant effect on JH biosynthesis in vitro in our previous study.Functional evaluation demonstrated that all the target compounds can activate the Dippu-Ast R,albeit with different potencies.Analog L6 with the longest linker（n=5）,exhibited not only a promising effect on inhibition of JH biosynthesis both in vitro and in vivo,but also good activity in inhibiting basal oocyte growth.Structure–activity relationships（SAR）studies showed that longer linkers provided greater contribution to activity.展开更多
The cell cycle is an important research field in cell biology and it is genetically and developmentally regulated in animals and plants. The aim of this study was to review knowledge about the biochemical regulation o...The cell cycle is an important research field in cell biology and it is genetically and developmentally regulated in animals and plants. The aim of this study was to review knowledge about the biochemical regulation of the cell cycle by plant growth regulators through molecular checkpoints that regulate the transition from G0-G1-S-phase and G2-M in higher plants. Recent research has shown that zeatin treatment led to the up-regulation of CycD3 in Arabidopsis. Benzyladenine treatment can also shorten the duration of S-phase through recruitment of latent origins of DNA replication. Kinetin is involved in the phosphoregulation of the G2-M checkpoint; the major cyclin-dependent kinase (Cdk) at this checkpoint has recently shown to be dephosphorylated as a result of cytokinin treatment, an effect that can also be mimicked by the fission yeast Cdc25 phos-phatase. Gibberellic acid (GA) treatment induces internode elongation in deepwater rice, this response is mediated by a GA-induced up-regulation of a cyclin-Cdk at the G2-M checkpoint. Recent evidence has also linked abscisic acid to a cy-clin-dependent kinase inhibitor. A new D-type cyclin, recently discovered in Arabidopsis may have a key role in this process. A brief review on plant growth regulator-cell cycle interfacing during development and a cytokinin-induced continuum of cell cycle activation through the up-regulation of a plant D-type cyclin at the G1 checkpoint and the phosphoregulation of the Cdk at the G2/M checkpoint had been concluded. This review could be valuable to research on cell and developmental biology in plants.展开更多
To improve fruit set in ＂Carmel＂ almond （Prunus dulcis） trees grown in the 6th region of Chile, seven different growth regulators （GRs） were sprayed onto flowering branches： gibberellic acid （GA3）, Promalin, ...To improve fruit set in ＂Carmel＂ almond （Prunus dulcis） trees grown in the 6th region of Chile, seven different growth regulators （GRs） were sprayed onto flowering branches： gibberellic acid （GA3）, Promalin, naphthaleneacetic acid （NAA）, Thidiazuron, Kinetin, Point-tomatomone and Brassinolide. The percentage of fruit set was determined by comparing the initial number of flowers present on each treated branch with the total number of almonds on the branches 50 days after anthesis. In comparison to the controls, Brassinolide, Point-tomatomone and Promalin achieved increases in fruit set of 19.4%, 14.5% and 12.9%, respectively. A parallel experiment was done in vitro, on the effects of these GRs on pollen germination and pollen tube growth. The greatest pollen germination （4 hours at 20 ~C） occurred with Brassinolide and GA3, which were significantly higher than the control, while NAA and Promalin significantly reduced germination. For pollen tube growth （24 hours at 20 ~C）, the greatest lengths were observed with Point-tomatomone and Brassinolide, which were significantly longer than the control, while NAA, Thidiazuron and Promalin were significantly shorter than the control, and the least growth was observed with Kinetin.展开更多
[Objective] The aim was to investigate the effects of plant growth regulators on contents of reducing sugar and starch in potato（Solanum tuberosum L.）.[Method]A potato cultivar named ＇Holand-212＇ with three treatm...[Objective] The aim was to investigate the effects of plant growth regulators on contents of reducing sugar and starch in potato（Solanum tuberosum L.）.[Method]A potato cultivar named ＇Holand-212＇ with three treatments by spraying Diethyl aminoethyl hexanoate（DTA-6）,Uniconazole（S3307）and SOD mimics（SODM）were employed to compare differences of the contents of reducing sugar and starch in potato leaves,stolons and tubers in a field experiment.[Result]DTA-6 treatment could significantly reduce the contents of starch and reducing sugar in potato stolons in the period of 100-110 d after seeding,and it could also reduce the content of reducing sugar in tubers,but there is an opposite result in leaves in the same period,significantly.S3307 could extremely significantly increase the content of starch,and can promote the transfer of starch in stolons,in addition,it also could reduce the content of reducing sugar in tubers,and extremely significantly increase the content of starch in leaves at the 110 d after sowing.Moreover,there is a similar effect between SODM and S3307.[Conclusion]The research results are significant for understanding mechanisms of plant growth regulators and promoting application of plant growth regulator in potato.展开更多
Plant growth regulators(PGRs)play an important role in increasing crop yield,and quality,and enhancing crop stress resistance in agricultural production,especially for important crops.PGRs can affect the transport and...Plant growth regulators(PGRs)play an important role in increasing crop yield,and quality,and enhancing crop stress resistance in agricultural production,especially for important crops.PGRs can affect the transport and distribution of assimilates by changing the content and distribution of endogenous hormones in plants.Numerous empirical research results have proven that PGRs have an important impact on the growth,development,and yield composition of wheat.Taking wheat plants as an example,this study reviews the application of PGRs in wheat production and explores their impact on wheat growth and yield.Furthermore,residues and microbial degradation of PGRs are summarized in detail.Finally,future research directions on PGR application in wheat production are proposed.This summary is of great significance for understanding the role of PGRs in wheat production.展开更多
Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three bi...Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S.dollars in the recent,and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types.Effects of plant growth regulators,culture media strength and photoperi-odic duration on the micropropagation ef ciency of C.fragrans from nodal segment explants were studied on Murashige and Skoog(MS)medium amended with Thidiazuron(TDZ),Benzylaminopurine(BAP)or Kinetin(Kin).Thidiazuron was more ef cient over BAP and Kin when half basal MS medium was used over full or quarter strength.Results of carbon source experiment showed sucrose as the most effective over glucose,fructose,and maltose in the clonal production.Studies on the photope-riodic incubation duration showed 12 h as the best light period and sub or supra-optimal resulted in the production of abnormal and albino micro shoots.Experimental results on the evaluation of physiological,biochemical parameters showed the role of pigment molecules and antioxidant systems in the production of albino micro shoots.展开更多
Vernonia amygdalina Del. is a tropical species susceptible to frost which is an annual phenomenon of the winter season of the Eastern Cape. Limited populations of the species arc in the province dieback during the win...Vernonia amygdalina Del. is a tropical species susceptible to frost which is an annual phenomenon of the winter season of the Eastern Cape. Limited populations of the species arc in the province dieback during the winter period, thereby making large scale cultivation through conventional vegetative propagation impossible. In our effort to increasing the population of the species within the province, a micropropagation approach through tissue culture teehnology was employed. Two growth regulators （BAP ＆ NAA） were tested at four levels of concentration under continuous light and dark conditions. Plantlets were subsequently transferred to the field after acclimatization at different temperature conditions. The result of the study showed that BAP generally performs better than NAA for callus induction under continuous darkness, while direct micropropagation of shoot under continuous light condition at I mg L^-1BAP showed the best result. To achieve greater success for the rapid multiplication of the species, this study further demonstrates that the optimum temperature range for acclimatizing the species prior to the transfer of the plantlet to the field is between 23 ±3 ℃.展开更多
基金The financial support from the National Key Research and Development Program of China(No.2023YFD1700600)。
文摘Agrochemicals,especially plant growth regulators(PGRs),are extensively used to modulate endogenous phytohormone signals in small quantities,significantly infiuencing plant growth and development.Plant hormones typically exhibit diverse chemical structures,with common examples including indole rings,terpenoid frameworks,adenine motifs,cyclic lactones,cyclopentanones,and steroidal compounds,which are extensively employed in pesticides.This article explores the interactions and biological activities of small molecules on proteins,enzymes,and other reactive sites involved in the biosynthesis,metabolism,transport,and signal transduction pathways of various plant hormones.Additionally,it analyzes the structure-activity relationships(SARs)of pesticides incorporating these structural motifs to elucidate the relationship between active fragments,pharmacophores,and targets,highlighting the characteristics of potent small molecules and their derivatives.This comprehensive review aims to provide novel perspectives for the development and design of pesticides,offering valuable insights for researchers in the field.
基金Supported by Key Technology Integration and Demonstration of Xinjiang Characteristic Fruit Trees'High Efficiency and Safe Production,Science and Technical Plan Project of Xinjiang Uygur Autonomous Region(201130102)Key Technology Integration and Demonstration of Xinjiang Apricot Industrial Development,Science and Technical Plan Project of Xinjiang Uygur Autonomous Region(200931101)Financial Support from Xinjiang Uygur Autonomous Region Fruit Trees Key Subject~~
文摘[Objective] This study aimed to investigate the influence of different plant growth regulators on apricot pollen germination and pollen tube growth. [Method] Pollens of six kinds of Xinjiang apricots were cultured in solid media supplemented with five plant growth regulators （GA3 , NAA, 2, 4-D, 6-BA, IAA）. Then the rate of pollen germination and the length of pollen tube were respectively measured. [Result] In a certain concentration range, GA3 most significantly promoted the pollen germination and the pollen tube growth of Shushanggan, Kalayulvke, Dayoujia, Yiliakeyulvke and Kabakehuanna; NNA had the strongest improvement function on Kumaiti’s pollen germination and pollen tube growth. [Conclusion] All the five plant growth regulators promoted the pollen germination and the pollen tube growth of apricots at low concentration but inhibited them at high concentration.
文摘The effects of four kinds of plant growth regulators with different concentrations on narcissi were studied in 2001. The results showed that the regulators could inhibit the growths of height and leaves of narcissi. Of the four regulators, the dwarfing effects of paclobatrazol (PP333) and uniconazole (S3307) on narcissi were better than those of chlorocholine (CCC) and dimethyl amino-sussinamic acid (B9). All of the regulators did not have significant effect on the root length. Moreover, the time of flowering was later for the narcissi treated with regulators than that of the control to a certain extent, and the range delayed was from 2 days to 19 days. The correlation analysis results showed that there was a significant correlation between the time of flowering and the concentrations of regulators. The ornament value of narcissi was obviously improved by using the regulators.
文摘Background Zonal application maps are designed to represent field variability using key variables that can be translated into tailored management practices.For cotton,zonal maps for crop growth regulator(CGR)applications under variable-rate(VR)strategies are commonly based exclusively on vegetation indices(VIs)variability.However,VIs often saturate in dense crop vegetation areas,limiting their effectiveness in distinguishing variability in crop growth.This study aimed to compare unsupervised framework(UF)and supervised framework(SUF)approaches for generat-ing zonal application maps for CGR under VR conditions.During 2022-2023 agricultural seasons,an UF was employed to generate zonal maps based on locally collected field data on plant height of cotton,satellite imagery,soil texture,and phenology data.Subsequently,a SUF(based on historical data between 2020-2021 to 2022-2023 agricultural seasons)was developed to predict plant height using remote sensing and phenology data,aiming to replicate same zonal maps but without relying on direct field measurements of plant height.Both approaches were tested in three fields and on two different dates per field.Results The predictive model for plant height of SUF performed well,as indicated by the model metrics.However,when comparing zonal application maps for specific field-date combinations,the predicted plant height exhibited lower variability compared with field measurements.This led to variable compatibility between SUF maps,which utilized the model predictions,and the UF maps,which were based on the real field data.Fields characterized by much pronounced soil texture variability yielded the highest compatibility between the zonal application maps produced by both SUF and UF approaches.This was predominantly due to the greater consistency in estimating plant development patterns within these heterogeneous field environments.While VR application approach can facilitate product savings during the application operation,other key factors must be considered.These include the availability of specialized machinery required for this type of applications,as well as the inherent operational costs associated with applying a single CGR product which differs from the typical uniform rate applications that often integrate multi-ple inputs.Conclusion Predictive modeling shows promise for assisting in the creation of zonal application maps for VR of CGR applications.However,the degree of agreement with the actual variability in crop growth found in the field should be evaluated on a field-by-field basis.The SUF approach,which is based on plant heigh prediction,demonstrated potential for supporting the development of zonal application maps for VR of CGR applications.However,the degree to which this approach aligns itself with the actual variability in crop growth observed in the field may vary,necessi-tating field-by-field evaluation.
文摘This paper reviewed the toxicity,maximum residue limits(MRLs)and current residue status of commonly used plant growth regulators in vegetables,including 2,4-dichlorophenoxyacetic acid(2,4-D),naphthaleneacetic acid(NAA),ethephon,gibberellin,and paclobutrazol.Methods for reducing residues of plant growth regulators in vegetables were discussed,and recommendations and strategies for their application were proposed.
文摘The effect of vernalization and two growth regulators Fascination^TM and Pro-Gibb^R on the growth, inflorescence development and flowering of Omithogalurrt Chesapeake Snowflake＇ was studied. Regardless of growth regulator treatment, chilling bulbs for 3 weeks at 10℃ before planting accelerated flowering of the first inflorescence by 5 to 6 days, elongated floral stem length by 1.5 to 2 cm and shortened leaf length by 4 to 5 cm as compared with non-chilled bulbs. When bulbs were chilled 3 weeks at 10℃ before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 2 to 6 days respectively as compared with the control. When bulbs were not chilled before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 6 to12 days respectively as compared with the control. The lengths of the leaves and the inflorescences were not affected by PGR treatment. Higher rates of Pro-Gibb （100 mg.L^-1 and 200 mg.L^-1） and more amount of Fascination 2%（100 μL and 200 μL） can cause abortion of inflorescence.
基金Supported by Special Fund for Yancheng Agricultural Science and Technology Innovation(YK2013012)~~
文摘The research reviewed use effects of Yuhuangjin, Xishibao, Zhuangfengling and Jianzhuangsu on Jinhai No. 5. The results showed plant height and ear height declined in varying degrees, as well as empty-stalk rate and lodging rate, with the plant growth regulators applied. Economic characters all improved, including ear length and diameter, barren-tip length and hundred-seed weight, and corn yield went up significantly on average. For example, the increased yield can be as high as 17.43% when Yuhuangjin was applied at 30 ml/hm^2.
文摘[Objective]In order to increase anther culture efficiency of pepper.[Method]MS culture media and Bolajiaohong were used in this experiment to study the influences of carbon sources and concentrations on anther callus induction of pepper.Jiayu was taken as a material to study influences of plant growth regulators and concentrations on anther callus induction of pepper according to L16（4^5） orthogonal design.[Result]The average callus and embryoid induction rates of maltose at all concentrations were higher than these of sucrose but the difference was not significant.Taking maltose or sucrose as a carbon source,3% to 6% concentration was good for increasing induction frequencies of calli and embryoids.However,If the concentration was over 6%,the induction rates were declined dramatically with the increase of sugar concentration.The influences of growth regulators on induction rate of calli were listed as 2,4-D﹥ZT﹥NAA﹥KT﹥6-BA;the influences on induction rates of embryoids were listed as 2,4-D﹥NAA﹥ZT﹥KT﹥6-BA.The 2,4-D,ZT,NAA and KT had signficant or extremely significant influences on induction rates of calli and embryoids.2,4-D,ZT at 1.0 mg/L and NNA,KT at 0.5 mg/L had the best effects.The influences of ZT on calli and embryoids were better than those of KT and 6-BA.1.0 mg/L 2,4-D ＋1.0 mg/L ZT ＋0.5 mg/L KT ＋0.5 mg/L 6-BA was the best regulator combination for induction culture of Jiayu anther.[Conclusion]The experiment provided research basis for anther culture of pepper.
基金supported by the Universiti Putra Malaysia Research University Grant Scheme(Project No.03-02-11-1370RU and 03-03-11-1438RU)
文摘The endangered tropical tree, Aquilaria malaccensis, produces agarwood for use in fragrance and medicines. Efforts are currently un-derway to produce valuable agarwood compoundsn tissue culture. The purpose of this study was to develop an optimal growth medium, specif-ically, the best hormone combination for callus suspension culture. Using nursery-grown A. malaccensis, sterilized leaf explants were first incu-bated on basic Murashige and Skoog （MS） gel medium containing 15g/L sucrose and at pH 5.7. Different auxin types including 1-naphthaleneacetic acid （NAA）, 2,4-dichlorophenoxyacetic acid （2,4-D）, and indole-3-butyric acid （IBA）, were tested at various concentrations （0.55, 1.1 and 1.65 μM） using the basic medium. Leaf explants were incubated for 30 days in the dark. Callus induced by 1.1 μM NAA had the highest biomass dry weight （DW） of 17.3 mg;however the callus was of a compact type. This auxin concentration was then combined with either 6-benzylaminopurine （BAP） or kinetin at 0.55, 1.1, 2.2 or 3.3 μM to induce growth of friable callus. The 1.1μM NAA＋2.2μM BAP com-bination produced friable callus with the highest biomass （93.3mg DW）. When testing the different carbon sources and pHs, sucrose at 15g/L and pH at 5.7 yielded highest biomasses at 87.7mg and 83mg DW, respec-tively. Microscopic observations revealed the arrangement of the friable cells as loosely packed with relatively large cells, while for the compact callus, the cells were small and densely packed. We concluded that MS medium containing 15 g/L sucrose, 1.1 μM NAA ＋ 2.2 μM BAP hor-mone combination, and a pH of 5.7 was highly effective for inducing friable callus from leaf explants of A. malaccensis for the purpose of establishing cell suspension culture.
文摘Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs.
基金Supported by National Key Technology Support Program(2012BAD19B01)
文摘Joint toxicity of Beauveria bassiana and two insect growth regulators, methoxyfenozide and hexaflumuron, on beet annywonn larvae, Spodoptera exigua （ Htthner）, were investigated. An obvious synergism effect of the two insect growth regulators with B. beauveria was observed. When the insect growth regulators at LC50 concentrations （25 × 10^-6 g/L for methoxyfenozide and 1 000 × 10^ -6 g/L for hexaflumuren） were mixed with B. bassiana at the volume ratio of 4： 1, the mixtures achieved the best efficacy. The co-toxicity co-efficient （CTC） of methexyfenozide and B. bass/ana was 285, and that of hexaflumuron and B. bass/ana was 208. Scanning electron microscope observation of the longitudinal section of S. exigua revealed obviously different patterns of integument in different treatment groups.
基金Supported by Talent Introduction Project of Anhui Science and Technology University(ZRC2007147)General Project of Natural Scientific Research from Education Department of Anhui Province(KJ2010B052,KJ2010B294)
文摘In this study,the seeds of wild Petunia Juss.were used as explants to investigate the optimal condition for tissue culture.Several different kinds and concentrations of growth regulators were adopted to produce more multiple bud clumps,callus or roots in this study.The experiments may provide experimental foundation for the rapid propagation technology and establishment of tissue culture system for wild Petunia Juss.
基金This work was supported by The Rufford Small Grants Foundation to Niemenak Nicolas(RSG Ref.70.05.09)and by the UNESCO 1’Oreal Fellowship for Women in Science Program to Nzweundji Justine Germo.The Alexander von Humboldt Foundation is also acknowledged for material donation to Niemenak Nicolas.
文摘Prunus africana is an endangered medicinal species and has been classified as a priority for domestication in Cameroon.However,the seeds rapidly lose their viability during storage at room temperature after 2–3 months.This study aimed to improve seed germination of P.africana by germinating at different temperatures(4℃,28℃)using different concentrations of growth stimulators(sodium nitrate and gibberellic acid)and different concentrations of salts.P.africana seeds had 91.7%germination at 4C after 1 month of storage.Growth regulators considerably influenced germination after 6 months and reached 66.0%with 10 mM gibberellic acid and 100%with 10 mM sodium nitrate.Approximately three shoots per seed were developed,an indication of polyembryony.Histochemical analyses revealed the presence of protein-like bodies close to the embryo axis and accumulations of starch after 7 days of germination.After 14 days,amyloplasts and dark protein bodies of various sizes were observed.The outcome of this study will contribute to improve the germination of P.africana for better domestication and conservation.
基金supported by the National Natural Science Foundation of China (No. 21372257)the National Basic Research Program of China (973 Program, No. 2010CB126104)+1 种基金the Natural Sciences and Engineering Research Council of Canadasupported by China Scholarship Council (CSC) to study in the laboratory of SST at University of Toronto,Canada
文摘Insect growth regulators play an important role in integrated pest management strategies.The FGLa–allatostatins（ASTs）are a family of neuropeptides that can inhibit juvenile hormone（JH）biosynthesis by the corpora allata（CA）of Diploptera punctata in vitro,are regarded as insect growth regulator candidates.In the search for new potential mimics and to explore the effect of linker length on inhibiting JH biosynthesis,a series of AST analogs were synthesized by modifying the linker of K24,which was found to have a significant effect on JH biosynthesis in vitro in our previous study.Functional evaluation demonstrated that all the target compounds can activate the Dippu-Ast R,albeit with different potencies.Analog L6 with the longest linker（n=5）,exhibited not only a promising effect on inhibition of JH biosynthesis both in vitro and in vivo,but also good activity in inhibiting basal oocyte growth.Structure–activity relationships（SAR）studies showed that longer linkers provided greater contribution to activity.
文摘The cell cycle is an important research field in cell biology and it is genetically and developmentally regulated in animals and plants. The aim of this study was to review knowledge about the biochemical regulation of the cell cycle by plant growth regulators through molecular checkpoints that regulate the transition from G0-G1-S-phase and G2-M in higher plants. Recent research has shown that zeatin treatment led to the up-regulation of CycD3 in Arabidopsis. Benzyladenine treatment can also shorten the duration of S-phase through recruitment of latent origins of DNA replication. Kinetin is involved in the phosphoregulation of the G2-M checkpoint; the major cyclin-dependent kinase (Cdk) at this checkpoint has recently shown to be dephosphorylated as a result of cytokinin treatment, an effect that can also be mimicked by the fission yeast Cdc25 phos-phatase. Gibberellic acid (GA) treatment induces internode elongation in deepwater rice, this response is mediated by a GA-induced up-regulation of a cyclin-Cdk at the G2-M checkpoint. Recent evidence has also linked abscisic acid to a cy-clin-dependent kinase inhibitor. A new D-type cyclin, recently discovered in Arabidopsis may have a key role in this process. A brief review on plant growth regulator-cell cycle interfacing during development and a cytokinin-induced continuum of cell cycle activation through the up-regulation of a plant D-type cyclin at the G1 checkpoint and the phosphoregulation of the Cdk at the G2/M checkpoint had been concluded. This review could be valuable to research on cell and developmental biology in plants.
文摘To improve fruit set in ＂Carmel＂ almond （Prunus dulcis） trees grown in the 6th region of Chile, seven different growth regulators （GRs） were sprayed onto flowering branches： gibberellic acid （GA3）, Promalin, naphthaleneacetic acid （NAA）, Thidiazuron, Kinetin, Point-tomatomone and Brassinolide. The percentage of fruit set was determined by comparing the initial number of flowers present on each treated branch with the total number of almonds on the branches 50 days after anthesis. In comparison to the controls, Brassinolide, Point-tomatomone and Promalin achieved increases in fruit set of 19.4%, 14.5% and 12.9%, respectively. A parallel experiment was done in vitro, on the effects of these GRs on pollen germination and pollen tube growth. The greatest pollen germination （4 hours at 20 ~C） occurred with Brassinolide and GA3, which were significantly higher than the control, while NAA and Promalin significantly reduced germination. For pollen tube growth （24 hours at 20 ~C）, the greatest lengths were observed with Point-tomatomone and Brassinolide, which were significantly longer than the control, while NAA, Thidiazuron and Promalin were significantly shorter than the control, and the least growth was observed with Kinetin.
基金Supported by Daqing Scientific and Technological Project in Heilongjiang Province(SGG2007-054)Demonstration and Promotion Projects of Potato Comprehensive High Yield Cultivation Technologyin Western Regions of Heilongjiang Province(PC07B09)~~
文摘[Objective] The aim was to investigate the effects of plant growth regulators on contents of reducing sugar and starch in potato（Solanum tuberosum L.）.[Method]A potato cultivar named ＇Holand-212＇ with three treatments by spraying Diethyl aminoethyl hexanoate（DTA-6）,Uniconazole（S3307）and SOD mimics（SODM）were employed to compare differences of the contents of reducing sugar and starch in potato leaves,stolons and tubers in a field experiment.[Result]DTA-6 treatment could significantly reduce the contents of starch and reducing sugar in potato stolons in the period of 100-110 d after seeding,and it could also reduce the content of reducing sugar in tubers,but there is an opposite result in leaves in the same period,significantly.S3307 could extremely significantly increase the content of starch,and can promote the transfer of starch in stolons,in addition,it also could reduce the content of reducing sugar in tubers,and extremely significantly increase the content of starch in leaves at the 110 d after sowing.Moreover,there is a similar effect between SODM and S3307.[Conclusion]The research results are significant for understanding mechanisms of plant growth regulators and promoting application of plant growth regulator in potato.
基金supported by the Anhui Natural Science Foundation Project(No.2008085MB45).
文摘Plant growth regulators(PGRs)play an important role in increasing crop yield,and quality,and enhancing crop stress resistance in agricultural production,especially for important crops.PGRs can affect the transport and distribution of assimilates by changing the content and distribution of endogenous hormones in plants.Numerous empirical research results have proven that PGRs have an important impact on the growth,development,and yield composition of wheat.Taking wheat plants as an example,this study reviews the application of PGRs in wheat production and explores their impact on wheat growth and yield.Furthermore,residues and microbial degradation of PGRs are summarized in detail.Finally,future research directions on PGR application in wheat production are proposed.This summary is of great significance for understanding the role of PGRs in wheat production.
基金supported by the Department of Biotechnology,Government of India New Delhithe World Academy of Science(TWAS)for the Advancement of Science in the Developing World TriesteItaly through DBT-TWAS Postgraduate Research Fellowship
文摘Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S.dollars in the recent,and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types.Effects of plant growth regulators,culture media strength and photoperi-odic duration on the micropropagation ef ciency of C.fragrans from nodal segment explants were studied on Murashige and Skoog(MS)medium amended with Thidiazuron(TDZ),Benzylaminopurine(BAP)or Kinetin(Kin).Thidiazuron was more ef cient over BAP and Kin when half basal MS medium was used over full or quarter strength.Results of carbon source experiment showed sucrose as the most effective over glucose,fructose,and maltose in the clonal production.Studies on the photope-riodic incubation duration showed 12 h as the best light period and sub or supra-optimal resulted in the production of abnormal and albino micro shoots.Experimental results on the evaluation of physiological,biochemical parameters showed the role of pigment molecules and antioxidant systems in the production of albino micro shoots.
文摘Vernonia amygdalina Del. is a tropical species susceptible to frost which is an annual phenomenon of the winter season of the Eastern Cape. Limited populations of the species arc in the province dieback during the winter period, thereby making large scale cultivation through conventional vegetative propagation impossible. In our effort to increasing the population of the species within the province, a micropropagation approach through tissue culture teehnology was employed. Two growth regulators （BAP ＆ NAA） were tested at four levels of concentration under continuous light and dark conditions. Plantlets were subsequently transferred to the field after acclimatization at different temperature conditions. The result of the study showed that BAP generally performs better than NAA for callus induction under continuous darkness, while direct micropropagation of shoot under continuous light condition at I mg L^-1BAP showed the best result. To achieve greater success for the rapid multiplication of the species, this study further demonstrates that the optimum temperature range for acclimatizing the species prior to the transfer of the plantlet to the field is between 23 ±3 ℃.
