Gomphus purpuraceus is an ancient wild edible mushroom.However,the niche and lignocellulose degradation system of G.purpuraceus have remained cryptic.In the current study,transcriptome and proteome sequencing of G.pur...Gomphus purpuraceus is an ancient wild edible mushroom.However,the niche and lignocellulose degradation system of G.purpuraceus have remained cryptic.In the current study,transcriptome and proteome sequencing of G.purpuraceus in four mediums was performed.A total of 3489 differentially expressed genes(DEGs)and 891 differentially expressed proteins(DEPs)were screened out.For the lignocellulose degradation system,a total of 61 expressed genes were annotated,including 19 genes encoding cellulase,8 genes encoding hemicellulase,5 genes encoding pectinase,22 genes encoding lignin oxidase,and 7 lignin degrading auxiliary enzyme.From two-omics,morphology,and enzyme activities,we speculated that G.purpuraceus might have a limited capacity to degrade polysaccharides(starch and lignocellulose).In addition,trace amount of fumonisins(FB_(1)and FB_(2))were found in the G.purpuraceus.These results provided a new perspective for understanding the key pathways and hub genes involved in carbon metabolism of G.purpuraceus development.展开更多
基金the Science and Technology Program of Guizhou Province(2022-General 112)the Major Scientific and Technological Special Project of Guizhou Province(20193006-2)the Regional Fund Project of National Natural Science Foundation of China(32460795)。
文摘Gomphus purpuraceus is an ancient wild edible mushroom.However,the niche and lignocellulose degradation system of G.purpuraceus have remained cryptic.In the current study,transcriptome and proteome sequencing of G.purpuraceus in four mediums was performed.A total of 3489 differentially expressed genes(DEGs)and 891 differentially expressed proteins(DEPs)were screened out.For the lignocellulose degradation system,a total of 61 expressed genes were annotated,including 19 genes encoding cellulase,8 genes encoding hemicellulase,5 genes encoding pectinase,22 genes encoding lignin oxidase,and 7 lignin degrading auxiliary enzyme.From two-omics,morphology,and enzyme activities,we speculated that G.purpuraceus might have a limited capacity to degrade polysaccharides(starch and lignocellulose).In addition,trace amount of fumonisins(FB_(1)and FB_(2))were found in the G.purpuraceus.These results provided a new perspective for understanding the key pathways and hub genes involved in carbon metabolism of G.purpuraceus development.
