Natural colored cotton(NCC)offers a sustainable,dye-free,and eco-friendly alternative for producing colored textiles.Carotenoids,a group of important natural liposoluble pigments,are known for their diverse color spec...Natural colored cotton(NCC)offers a sustainable,dye-free,and eco-friendly alternative for producing colored textiles.Carotenoids,a group of important natural liposoluble pigments,are known for their diverse color spectrum.In this study,we successfully engineered the carotenoid biosynthesis pathway specifically in cotton fibers by utilizing a fiber-specific GbEXPA2 promoter and a CaMV 35S promoter to drive the expression of two key carotenoid biosynthesis genes,CrtB and CrtI,respectively.This approach resulted in the development of a golden fiber cotton germplasm enriched withβ-carotene.Notably,the pigmentation was predominantly observed during the early developmental stages of the fiber(5–20 d post-anthesis).While the presence of carotenoids had no significant effect on plant architecture and growth,it positively influenced the fiber elongation rate,albeit with a slight reduction in fiber length and strength.This study represents a pioneering strategy for the future development of NCCs through carotenoid biofortification.展开更多
Background:The golden Syrian hamster is a valuable animal model for studying carcinogenesis,metabolic disorders,cardiovascular diseases,and viral infections due to its biological and pathological similarities to human...Background:The golden Syrian hamster is a valuable animal model for studying carcinogenesis,metabolic disorders,cardiovascular diseases,and viral infections due to its biological and pathological similarities to humans.However,the development of genetically engineered hamsters has lagged behind that of mice and rats,largely because of an embryonic development block at the two-cell stage in vitro.Although CRISPR/Cas9-mediated gene knockout has been achieved in hamsters,precise DNA fragment insertion or conditional knockout(cKO)models have not previously been reported,likely due to technical limitations in embryo manipulation and insufficient efficiency of homology-directed repair(HDR).Methods:In this study,we generated conditional alleles of the ApoF gene in golden Syrian hamsters.A two-cut strategy was applied using Cas9 protein,two sgRNAs,and a single donor plasmid containing exon 2 flanked by loxP sites and two~0.8 kb homology arms.A mixture of Cas9 protein,sgRNAs,and the donor plasmid was microinjected into the pronuclei of one-cell stage hamster embryos.Results:The efficiency of CRISPR/Cas9-mediated loxP knock-in reached up to 27%,and the genetically modified floxed alleles were successfully transmitted through the germline.The functionality of the inserted loxP sites was validated by in vivo Cremediated recombination following local administration of AAV vectors,including AAV-cTnT-Cre in the heart and AAV-CMV-Cre in the brain.Conclusions:To our knowledge,this work represents the first successful establishment of a conditional knockout model in the golden Syrian hamster,providing a valuable tool for mechanistic studies of gene function and disease modeling.展开更多
Ming-style furniture represents the pinnacle of Chinese traditional furniture,with the four-head official’s cap chair standing out as a representative category due to its classic form and cultural connotations.This p...Ming-style furniture represents the pinnacle of Chinese traditional furniture,with the four-head official’s cap chair standing out as a representative category due to its classic form and cultural connotations.This paper takes a specific rosewood four-head official’s cap chair inscribed by Wang Shixiang as the research subject,employing a tripartite approach of form analysis,material examination,and inscription interpretation.The chair strictly adheres to the classic form of Ming-style four-head official’s cap chairs,embodying the Ming Dynasty design philosophy of“the unity of function and aesthetics”.The scarcity and unique grain pattern of the golden-flecked rosewood(Zitan)endow it with the status of“gold among woods”.Wang Shixiang’s inscription binds the furniture to the cultural essence of“the charm of the Ming Dynasty”,transforming it into“heritable cultural heritage”.This paper provides a new perspective for the case study of Ming-style furniture and offers theoretical reference for the contemporary inheritance of traditional craftsmanship.展开更多
From December 13 to 17, 2025, the 2025 Training Program for Future Lancang-Mekong Cooperation (LMC) Diplomats themed “Embarking on a New Golden Decade:Fostering a Sense of the Lancang-Mekong Family” was held in Haik...From December 13 to 17, 2025, the 2025 Training Program for Future Lancang-Mekong Cooperation (LMC) Diplomats themed “Embarking on a New Golden Decade:Fostering a Sense of the Lancang-Mekong Family” was held in Haikou,south China’s Hainan Province. The program was conducted under the guidance of the Research Center on Building a Community with a Shared Future for Mankind and jointly organized by China Foreign Affairs University and China Institute for Reform and Development. The event brought together over 80 outstanding participants from the Lancang-Mekong countries to draft an initiative to submit to the LMC China Secretariat.展开更多
This year marks the 65th anniversary of diplomatic relations between China and Laos.As neighbors sharing a common border,the two nations are comrades and brothers.When China proposed the vision of building a community...This year marks the 65th anniversary of diplomatic relations between China and Laos.As neighbors sharing a common border,the two nations are comrades and brothers.When China proposed the vision of building a community with a shared future for humanity,Laos became the first to reach political consensus on establishing such a bilateral community with China.展开更多
Inoculated fermentation enables rapid fermentation of aquatic products.No studies have been conducted on the lipid profiles of inoculated fermented golden pompano(Trachinotus ovatus).In this study,a lipase-producing B...Inoculated fermentation enables rapid fermentation of aquatic products.No studies have been conducted on the lipid profiles of inoculated fermented golden pompano(Trachinotus ovatus).In this study,a lipase-producing Bacillus subtilis with salt tolerance was screened from traditionally fermented golden pompano(TF)and used as a starter culture.Whole-genome sequencing analysis revealed it carries 4 clustered regularly interspaced short vpalindromic repeats structures and 2 genes encoding triacylglycerol lipase.Untargeted lipidomics identified lipid molecules(833)in 6 major classes from B.subtilis SCSMX-2 fermented golden pompano(IF).A total of 28 lipid molecules were upregulated in IF,including phosphatidylcholines(PCs),triacylglycerols(TAGs),and lysophosphatidylcholine.B.subtilis supplementation enhanced the production of polyenyl PCs and mediumand long-chain TAGs.The IF rich in linoleic,docosahexaenoic acids(DHA),and eicosapentaenoic acid were primarily distributed in the sn-2 position of DHA,PC and phosphatidylethanolamine.This research revealed the lipid profiles of IF,providing theoretical basis for the application of B.subtilis in the fermented fish industry.展开更多
"Look at this!This one grows well and big.And its shape,so round and plump.How.adorable,"exclaimed Pema Chosphel as he carefully unearthed a palm-sized gastrodia from the damp soil.Overjoyed,he called his fa..."Look at this!This one grows well and big.And its shape,so round and plump.How.adorable,"exclaimed Pema Chosphel as he carefully unearthed a palm-sized gastrodia from the damp soil.Overjoyed,he called his father to share his excitement.展开更多
Nujiang farmers use the innovative dryland rice cultivation technology developed by a Chinese expert team to grow yields and income.As April ushers in spring,mist clings to the deep valleys of the Nujiang Grand Canyon...Nujiang farmers use the innovative dryland rice cultivation technology developed by a Chinese expert team to grow yields and income.As April ushers in spring,mist clings to the deep valleys of the Nujiang Grand Canyon in Southwest China’s Yunnan Province.High on a mountainside in Ziji Village,Daxingdi Town,Lushui City,Nujiang Lisu Autonomous Prefecture,at an altitude exceeding 1,300 metres,62-year-old Lisu villager Cha Wenxing is planting rice.These demonstration plots,cultivating high-quality upland rice,seem to float amongst the clouds.展开更多
This year marks the 50th anniversary of China-Thailand diplomatic relations as well as the“Golden Jubilee of China-Thailand Friendship.”From February 5 to 8,Thai Prime Minister Paetongtarn Shinawatra made an officia...This year marks the 50th anniversary of China-Thailand diplomatic relations as well as the“Golden Jubilee of China-Thailand Friendship.”From February 5 to 8,Thai Prime Minister Paetongtarn Shinawatra made an official visit to China.On February 8,the two sides issued the Joint Statement between the Government of the Kingdom of Thailand and the Government of the People’s Republic of China on Advancing the Comprehensive Strategic Cooperative Partnership and Building a Thailand-China Community with a Shared Future for Enhanced Stability,Prosperity,and Sustainability through a Forwardlooking and People-Centred Vision.展开更多
As part of events marking the 50th anniversary of diplomatic ties between China and Thailand,a forum themed“Our Golden Friendship:Joining Hands to Build a China-Thailand Community with a Shared Future”was held in Ba...As part of events marking the 50th anniversary of diplomatic ties between China and Thailand,a forum themed“Our Golden Friendship:Joining Hands to Build a China-Thailand Community with a Shared Future”was held in Bangkok on January 17,aiming to deepen mutual trust and enhance exchange between Chinese and Thai media and think tanks.展开更多
This year marks the 50th anniversary of the establishment of China-Thailand diplomatic relations and the“Golden Jubilee of China-Thailand Friendship.”Over the decades,fruitful results have been achieved in bilateral...This year marks the 50th anniversary of the establishment of China-Thailand diplomatic relations and the“Golden Jubilee of China-Thailand Friendship.”Over the decades,fruitful results have been achieved in bilateral cooperation in fields such as politics,economics,security,and cultural exchange.So,what are the key factors contributing to the enduring friendship and the remarkable achievements in bilateral cooperation?And,how does strengthened comprehensive strategic partnership and joint construction of a more stable,prosperous,and sustainable China-Thailand community with a shared future benefit both countries,the region,and even the world?展开更多
To address the problem of high lifespan loss and poor state of charge(SOC)balance of electric vehicles(EVs)participating in grid peak shaving,an improved golden eagle optimizer(IGEO)algorithm for EV grouping control s...To address the problem of high lifespan loss and poor state of charge(SOC)balance of electric vehicles(EVs)participating in grid peak shaving,an improved golden eagle optimizer(IGEO)algorithm for EV grouping control strategy is proposed for peak shaving sce-narios.First,considering the difference between peak and valley loads and the operating costs of EVs,a peak shaving model for EVs is constructed.Second,the design of IGEO has improved the global exploration and local development capabilities of the golden eagle optimizer(GEO)algorithm.Subsequently,IGEO is used to solve the peak shaving model and obtain the overall EV grid connected charging and discharging instructions.Next,using the k-means algorithm,EVs are dynamically divided into priority charging groups,backup groups,and priority discharging groups based on SOC differences.Finally,a dual layer power distribution scheme for EVs is designed.The upper layer determines the charging and discharging sequences and instructions for the three groups of EVs,whereas the lower layer allocates the charging and discharging instructions for each group to each EV.The proposed strategy was simulated and verified,and the results showed that the designed IGEO had faster optimization speed and higher optimization accuracy.The pro-posed EV grouping control strategy effectively reduces the peak-valley difference in the power grid,reduces the operational life loss of EVs,and maintains a better SOC balance for EVs.展开更多
Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate asse...Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate assembly technique,utilizing Escherichia coli as the model organism.Through polymerase chain reaction(PCR)amplification,restriction enzyme digestion,ligation,transformation,and Sanger sequencing,we assessed the operational efficiency and cloning fidelity of both strategies.Our results demonstrated that Golden Gate assembly,leveraging type IIS restriction enzymes and simultaneous ligation,significantly enhanced cloning efficiency and precision,particularly for seamless multi-fragment assembly.In contrast,the classical cloning approach maintained certain advantages in simplicity and robustness for specific experimental conditions.Challenges encountered during transformation and sequencing highlighted the critical impact of technical accuracy on experimental outcomes.This study underscores the importance of selecting appropriate cloning methodologies tailored to experimental objectives and laboratory capabilities,providing a foundation for optimized molecular cloning workflows in future synthetic biology and biotechnology applications.展开更多
In this paper,the necessity of constructing a golden course for Advanced Mathematics is presented.For teachers,golden courses enhance their teaching ability and job satisfaction.For students,golden courses improve the...In this paper,the necessity of constructing a golden course for Advanced Mathematics is presented.For teachers,golden courses enhance their teaching ability and job satisfaction.For students,golden courses improve their sense of learning value and significance and encourage them to actively learn and participate deeply.Next,the relevant content of course ideological and political construction is provided.Finally,a specific approach was proposed to create a golden course for Advanced Mathematics by integrating ideological and political education into the curriculum.展开更多
Reliable Cluster Head(CH)selectionbased routing protocols are necessary for increasing the packet transmission efficiency with optimal path discovery that never introduces degradation over the transmission reliability...Reliable Cluster Head(CH)selectionbased routing protocols are necessary for increasing the packet transmission efficiency with optimal path discovery that never introduces degradation over the transmission reliability.In this paper,Hybrid Golden Jackal,and Improved Whale Optimization Algorithm(HGJIWOA)is proposed as an effective and optimal routing protocol that guarantees efficient routing of data packets in the established between the CHs and the movable sink.This HGJIWOA included the phases of Dynamic Lens-Imaging Learning Strategy and Novel Update Rules for determining the reliable route essential for data packets broadcasting attained through fitness measure estimation-based CH selection.The process of CH selection achieved using Golden Jackal Optimization Algorithm(GJOA)completely depends on the factors of maintainability,consistency,trust,delay,and energy.The adopted GJOA algorithm play a dominant role in determining the optimal path of routing depending on the parameter of reduced delay and minimal distance.It further utilized Improved Whale Optimisation Algorithm(IWOA)for forwarding the data from chosen CHs to the BS via optimized route depending on the parameters of energy and distance.It also included a reliable route maintenance process that aids in deciding the selected route through which data need to be transmitted or re-routed.The simulation outcomes of the proposed HGJIWOA mechanism with different sensor nodes confirmed an improved mean throughput of 18.21%,sustained residual energy of 19.64%with minimized end-to-end delay of 21.82%,better than the competitive CH selection approaches.展开更多
A symmetrical quartic polynomial, named golden one, can be connected to coefficients of the icosahedron equation as well as to the gyromagnetic correction of the electron and to number 137. This number is not a mystic...A symmetrical quartic polynomial, named golden one, can be connected to coefficients of the icosahedron equation as well as to the gyromagnetic correction of the electron and to number 137. This number is not a mystic one, but is connected with the inverse of Sommerfeld’s fine-structure constant and this way again connected with the electron. From number-theoretical realities, including the reciprocity relation of the golden ratio as effective pre-calculator of nature’s creativeness, a proposed closeness to the icosahedron may point towards the structure of the electron, thought off as a single-strand compacted helically self-confined charged elemantary particle of less spherical but assumed blunted icosahedral shape generated from a high energy double-helix photon. We constructed a chiral Moebius “ball” from a 13 times 180˚twisted double helix strand, where the turning points of 12 generated slings were arranged towards the vertices of a regular icosahedron, belonging to the non-centrosymmetric rotation group I532. Mathematically put, we convert the helical motion of an energy quantum into a stationary motion on a Moebius stripe structure. The radius of the ball is about the Compton radius. This chiral closed circuit Moebius ball motion profile can be tentatively thought off as the dominant quantum vortex structure of the electron, and the model may be named CEWMB (Charged Electromagnetic Wave Moebius Ball). Also the gyromagnetic factor of the electron (g<sub>e</sub> = 2.002319) can be traced back to this special structure. However, nature’s energy infinity principle would suggest a superposition with additional less dominant (secondary) structures, governed also by the golden mean. A suggestion about the possible structure of delocalized hole carriers in the superconducting state is given.展开更多
The golden apple snail Pomacea canaliculata （Lamarck 1819） was first recorded at Black Dragon Spring, Dianchi Basin, Baiyi Township, Songming County, Kunming City, Yunnan Province, China, in October 2004. The water ...The golden apple snail Pomacea canaliculata （Lamarck 1819） was first recorded at Black Dragon Spring, Dianchi Basin, Baiyi Township, Songming County, Kunming City, Yunnan Province, China, in October 2004. The water from the spring flows into the Songhuaba Reservoir, the major drinking water resource for Kunming City, and part of the Dianchi Lake basin. This is the first record of this invasive snail in the Dianchi Lake Basin. Pomacea canaliculata originates from Central and South America, and in Asia the snail has spread through deliberate and accidental introductions to the Philippines, Vietnam, Thailand, Laos, Cambodia, Malaysia, Indonesia, Papua New Guinea, Korea, Japan and South China. It has become a major pest in rice-growing areas, resulting in huge damage to crops. Strict prevention and control measures have to be implemented to prevent the spread of the snail in Yunnan, together with public awareness campaigns to inform the public of the dangers of this invasive snail.展开更多
基金supported by grants from the National Natural Science Foundation of China(32170271,32470277)the Natural Science Foundation of Henan Province(222300420024).
文摘Natural colored cotton(NCC)offers a sustainable,dye-free,and eco-friendly alternative for producing colored textiles.Carotenoids,a group of important natural liposoluble pigments,are known for their diverse color spectrum.In this study,we successfully engineered the carotenoid biosynthesis pathway specifically in cotton fibers by utilizing a fiber-specific GbEXPA2 promoter and a CaMV 35S promoter to drive the expression of two key carotenoid biosynthesis genes,CrtB and CrtI,respectively.This approach resulted in the development of a golden fiber cotton germplasm enriched withβ-carotene.Notably,the pigmentation was predominantly observed during the early developmental stages of the fiber(5–20 d post-anthesis).While the presence of carotenoids had no significant effect on plant architecture and growth,it positively influenced the fiber elongation rate,albeit with a slight reduction in fiber length and strength.This study represents a pioneering strategy for the future development of NCCs through carotenoid biofortification.
基金State Key Laboratory Special Fund,Grant/Award Number:2060204Open Research Project in State Key Laboratory of Vascular Homeostasis and Remodeling,Grant/Award Number:Peking University,202411+3 种基金The Non-profit Central Research Institute Fund of the Chinese Academy of Medical Sciences,Grant/Award Number:2023-PT180-01Haihe Laboratory of Cell Ecosystem Innovation Fund,Grant/Award Number:HH24KYZX0007CAMS Innovation Fund for Medical Sciences,Grant/Award Number:2021-I2M-1-024,2022-I2M-1-020 and 2023-I2M-2-001the National Key Research and Development Program of China from the Ministry of Science and Technology,Grant/Award Number:2021YFF0702802。
文摘Background:The golden Syrian hamster is a valuable animal model for studying carcinogenesis,metabolic disorders,cardiovascular diseases,and viral infections due to its biological and pathological similarities to humans.However,the development of genetically engineered hamsters has lagged behind that of mice and rats,largely because of an embryonic development block at the two-cell stage in vitro.Although CRISPR/Cas9-mediated gene knockout has been achieved in hamsters,precise DNA fragment insertion or conditional knockout(cKO)models have not previously been reported,likely due to technical limitations in embryo manipulation and insufficient efficiency of homology-directed repair(HDR).Methods:In this study,we generated conditional alleles of the ApoF gene in golden Syrian hamsters.A two-cut strategy was applied using Cas9 protein,two sgRNAs,and a single donor plasmid containing exon 2 flanked by loxP sites and two~0.8 kb homology arms.A mixture of Cas9 protein,sgRNAs,and the donor plasmid was microinjected into the pronuclei of one-cell stage hamster embryos.Results:The efficiency of CRISPR/Cas9-mediated loxP knock-in reached up to 27%,and the genetically modified floxed alleles were successfully transmitted through the germline.The functionality of the inserted loxP sites was validated by in vivo Cremediated recombination following local administration of AAV vectors,including AAV-cTnT-Cre in the heart and AAV-CMV-Cre in the brain.Conclusions:To our knowledge,this work represents the first successful establishment of a conditional knockout model in the golden Syrian hamster,providing a valuable tool for mechanistic studies of gene function and disease modeling.
文摘Ming-style furniture represents the pinnacle of Chinese traditional furniture,with the four-head official’s cap chair standing out as a representative category due to its classic form and cultural connotations.This paper takes a specific rosewood four-head official’s cap chair inscribed by Wang Shixiang as the research subject,employing a tripartite approach of form analysis,material examination,and inscription interpretation.The chair strictly adheres to the classic form of Ming-style four-head official’s cap chairs,embodying the Ming Dynasty design philosophy of“the unity of function and aesthetics”.The scarcity and unique grain pattern of the golden-flecked rosewood(Zitan)endow it with the status of“gold among woods”.Wang Shixiang’s inscription binds the furniture to the cultural essence of“the charm of the Ming Dynasty”,transforming it into“heritable cultural heritage”.This paper provides a new perspective for the case study of Ming-style furniture and offers theoretical reference for the contemporary inheritance of traditional craftsmanship.
文摘From December 13 to 17, 2025, the 2025 Training Program for Future Lancang-Mekong Cooperation (LMC) Diplomats themed “Embarking on a New Golden Decade:Fostering a Sense of the Lancang-Mekong Family” was held in Haikou,south China’s Hainan Province. The program was conducted under the guidance of the Research Center on Building a Community with a Shared Future for Mankind and jointly organized by China Foreign Affairs University and China Institute for Reform and Development. The event brought together over 80 outstanding participants from the Lancang-Mekong countries to draft an initiative to submit to the LMC China Secretariat.
文摘This year marks the 65th anniversary of diplomatic relations between China and Laos.As neighbors sharing a common border,the two nations are comrades and brothers.When China proposed the vision of building a community with a shared future for humanity,Laos became the first to reach political consensus on establishing such a bilateral community with China.
基金supported by the National Natural Science Foundation of China(32372367)the earmarked fund for CARS(CARS-47)+1 种基金Natural Science Foundation of Hainan Provine(323CXTD391)the Special Scientific Research Funds for Central Non-profit Institutes and the Chinese Academy of Fishery Sciences(2020TD69)。
文摘Inoculated fermentation enables rapid fermentation of aquatic products.No studies have been conducted on the lipid profiles of inoculated fermented golden pompano(Trachinotus ovatus).In this study,a lipase-producing Bacillus subtilis with salt tolerance was screened from traditionally fermented golden pompano(TF)and used as a starter culture.Whole-genome sequencing analysis revealed it carries 4 clustered regularly interspaced short vpalindromic repeats structures and 2 genes encoding triacylglycerol lipase.Untargeted lipidomics identified lipid molecules(833)in 6 major classes from B.subtilis SCSMX-2 fermented golden pompano(IF).A total of 28 lipid molecules were upregulated in IF,including phosphatidylcholines(PCs),triacylglycerols(TAGs),and lysophosphatidylcholine.B.subtilis supplementation enhanced the production of polyenyl PCs and mediumand long-chain TAGs.The IF rich in linoleic,docosahexaenoic acids(DHA),and eicosapentaenoic acid were primarily distributed in the sn-2 position of DHA,PC and phosphatidylethanolamine.This research revealed the lipid profiles of IF,providing theoretical basis for the application of B.subtilis in the fermented fish industry.
文摘"Look at this!This one grows well and big.And its shape,so round and plump.How.adorable,"exclaimed Pema Chosphel as he carefully unearthed a palm-sized gastrodia from the damp soil.Overjoyed,he called his father to share his excitement.
文摘Nujiang farmers use the innovative dryland rice cultivation technology developed by a Chinese expert team to grow yields and income.As April ushers in spring,mist clings to the deep valleys of the Nujiang Grand Canyon in Southwest China’s Yunnan Province.High on a mountainside in Ziji Village,Daxingdi Town,Lushui City,Nujiang Lisu Autonomous Prefecture,at an altitude exceeding 1,300 metres,62-year-old Lisu villager Cha Wenxing is planting rice.These demonstration plots,cultivating high-quality upland rice,seem to float amongst the clouds.
文摘This year marks the 50th anniversary of China-Thailand diplomatic relations as well as the“Golden Jubilee of China-Thailand Friendship.”From February 5 to 8,Thai Prime Minister Paetongtarn Shinawatra made an official visit to China.On February 8,the two sides issued the Joint Statement between the Government of the Kingdom of Thailand and the Government of the People’s Republic of China on Advancing the Comprehensive Strategic Cooperative Partnership and Building a Thailand-China Community with a Shared Future for Enhanced Stability,Prosperity,and Sustainability through a Forwardlooking and People-Centred Vision.
文摘As part of events marking the 50th anniversary of diplomatic ties between China and Thailand,a forum themed“Our Golden Friendship:Joining Hands to Build a China-Thailand Community with a Shared Future”was held in Bangkok on January 17,aiming to deepen mutual trust and enhance exchange between Chinese and Thai media and think tanks.
文摘This year marks the 50th anniversary of the establishment of China-Thailand diplomatic relations and the“Golden Jubilee of China-Thailand Friendship.”Over the decades,fruitful results have been achieved in bilateral cooperation in fields such as politics,economics,security,and cultural exchange.So,what are the key factors contributing to the enduring friendship and the remarkable achievements in bilateral cooperation?And,how does strengthened comprehensive strategic partnership and joint construction of a more stable,prosperous,and sustainable China-Thailand community with a shared future benefit both countries,the region,and even the world?
基金supported by the National Natural Science Foundation of China(52077078)China Southern Power Grid Company Limited 036000KK52220004(GDKJXM20220147).
文摘To address the problem of high lifespan loss and poor state of charge(SOC)balance of electric vehicles(EVs)participating in grid peak shaving,an improved golden eagle optimizer(IGEO)algorithm for EV grouping control strategy is proposed for peak shaving sce-narios.First,considering the difference between peak and valley loads and the operating costs of EVs,a peak shaving model for EVs is constructed.Second,the design of IGEO has improved the global exploration and local development capabilities of the golden eagle optimizer(GEO)algorithm.Subsequently,IGEO is used to solve the peak shaving model and obtain the overall EV grid connected charging and discharging instructions.Next,using the k-means algorithm,EVs are dynamically divided into priority charging groups,backup groups,and priority discharging groups based on SOC differences.Finally,a dual layer power distribution scheme for EVs is designed.The upper layer determines the charging and discharging sequences and instructions for the three groups of EVs,whereas the lower layer allocates the charging and discharging instructions for each group to each EV.The proposed strategy was simulated and verified,and the results showed that the designed IGEO had faster optimization speed and higher optimization accuracy.The pro-posed EV grouping control strategy effectively reduces the peak-valley difference in the power grid,reduces the operational life loss of EVs,and maintains a better SOC balance for EVs.
文摘Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate assembly technique,utilizing Escherichia coli as the model organism.Through polymerase chain reaction(PCR)amplification,restriction enzyme digestion,ligation,transformation,and Sanger sequencing,we assessed the operational efficiency and cloning fidelity of both strategies.Our results demonstrated that Golden Gate assembly,leveraging type IIS restriction enzymes and simultaneous ligation,significantly enhanced cloning efficiency and precision,particularly for seamless multi-fragment assembly.In contrast,the classical cloning approach maintained certain advantages in simplicity and robustness for specific experimental conditions.Challenges encountered during transformation and sequencing highlighted the critical impact of technical accuracy on experimental outcomes.This study underscores the importance of selecting appropriate cloning methodologies tailored to experimental objectives and laboratory capabilities,providing a foundation for optimized molecular cloning workflows in future synthetic biology and biotechnology applications.
基金Project of Heilongjiang University of Finance and Economics(XJYB2024056)Project of Heilongjiang University of Finance and Economics(XJYB2024057)。
文摘In this paper,the necessity of constructing a golden course for Advanced Mathematics is presented.For teachers,golden courses enhance their teaching ability and job satisfaction.For students,golden courses improve their sense of learning value and significance and encourage them to actively learn and participate deeply.Next,the relevant content of course ideological and political construction is provided.Finally,a specific approach was proposed to create a golden course for Advanced Mathematics by integrating ideological and political education into the curriculum.
文摘Reliable Cluster Head(CH)selectionbased routing protocols are necessary for increasing the packet transmission efficiency with optimal path discovery that never introduces degradation over the transmission reliability.In this paper,Hybrid Golden Jackal,and Improved Whale Optimization Algorithm(HGJIWOA)is proposed as an effective and optimal routing protocol that guarantees efficient routing of data packets in the established between the CHs and the movable sink.This HGJIWOA included the phases of Dynamic Lens-Imaging Learning Strategy and Novel Update Rules for determining the reliable route essential for data packets broadcasting attained through fitness measure estimation-based CH selection.The process of CH selection achieved using Golden Jackal Optimization Algorithm(GJOA)completely depends on the factors of maintainability,consistency,trust,delay,and energy.The adopted GJOA algorithm play a dominant role in determining the optimal path of routing depending on the parameter of reduced delay and minimal distance.It further utilized Improved Whale Optimisation Algorithm(IWOA)for forwarding the data from chosen CHs to the BS via optimized route depending on the parameters of energy and distance.It also included a reliable route maintenance process that aids in deciding the selected route through which data need to be transmitted or re-routed.The simulation outcomes of the proposed HGJIWOA mechanism with different sensor nodes confirmed an improved mean throughput of 18.21%,sustained residual energy of 19.64%with minimized end-to-end delay of 21.82%,better than the competitive CH selection approaches.
文摘A symmetrical quartic polynomial, named golden one, can be connected to coefficients of the icosahedron equation as well as to the gyromagnetic correction of the electron and to number 137. This number is not a mystic one, but is connected with the inverse of Sommerfeld’s fine-structure constant and this way again connected with the electron. From number-theoretical realities, including the reciprocity relation of the golden ratio as effective pre-calculator of nature’s creativeness, a proposed closeness to the icosahedron may point towards the structure of the electron, thought off as a single-strand compacted helically self-confined charged elemantary particle of less spherical but assumed blunted icosahedral shape generated from a high energy double-helix photon. We constructed a chiral Moebius “ball” from a 13 times 180˚twisted double helix strand, where the turning points of 12 generated slings were arranged towards the vertices of a regular icosahedron, belonging to the non-centrosymmetric rotation group I532. Mathematically put, we convert the helical motion of an energy quantum into a stationary motion on a Moebius stripe structure. The radius of the ball is about the Compton radius. This chiral closed circuit Moebius ball motion profile can be tentatively thought off as the dominant quantum vortex structure of the electron, and the model may be named CEWMB (Charged Electromagnetic Wave Moebius Ball). Also the gyromagnetic factor of the electron (g<sub>e</sub> = 2.002319) can be traced back to this special structure. However, nature’s energy infinity principle would suggest a superposition with additional less dominant (secondary) structures, governed also by the golden mean. A suggestion about the possible structure of delocalized hole carriers in the superconducting state is given.
文摘The golden apple snail Pomacea canaliculata （Lamarck 1819） was first recorded at Black Dragon Spring, Dianchi Basin, Baiyi Township, Songming County, Kunming City, Yunnan Province, China, in October 2004. The water from the spring flows into the Songhuaba Reservoir, the major drinking water resource for Kunming City, and part of the Dianchi Lake basin. This is the first record of this invasive snail in the Dianchi Lake Basin. Pomacea canaliculata originates from Central and South America, and in Asia the snail has spread through deliberate and accidental introductions to the Philippines, Vietnam, Thailand, Laos, Cambodia, Malaysia, Indonesia, Papua New Guinea, Korea, Japan and South China. It has become a major pest in rice-growing areas, resulting in huge damage to crops. Strict prevention and control measures have to be implemented to prevent the spread of the snail in Yunnan, together with public awareness campaigns to inform the public of the dangers of this invasive snail.
