The eye is one of the important organs for human beings to obtain information from the outside world and avoid harm,and 90%of human knowledge is obtained through the eye.The center of the retina in the human eye is ca...The eye is one of the important organs for human beings to obtain information from the outside world and avoid harm,and 90%of human knowledge is obtained through the eye.The center of the retina in the human eye is called the macula,and it is the area where vision is most concentrated.Current research suggests that macular pigment is mainly composed of lutein,zeaxanthin,and meso-zeaxanthin,which are able to absorb and attenuate blue light damage to the retina,and since humans cannot synthesize these carotenoids,they must be obtained by supplementing with carotenoid-rich foods.In addition,carotenoids also provide good provitamin A,which provides necessary nutritional support for people’s visual transduction.Therefore,eye health is inseparable from carotenoid supplementation,among which wolfberry,which is rich in a variety of carotenoids,has been confirmed by recent studies to have a good role in promoting eye health,and also provides a good source of natural carotenoids for humans.However,a systematic review of goji berry carotenoids in eye health is lacking in current studies.Based on this,this paper reviews the main carotenoid composition and its extraction and purification in wolfberry,discusses the changes of carotenoids in the process of harvesting and drying wolfberry,reviews the absorption and utilization of carotenoids in wolfberry,and more importantly,discusses the important role of carotenoids in wolfberry in eye health.展开更多
Goji berry is a famous edible and medicinal substance around the world.In this research,15 phenylpropionyl phenylethylamine derivatives(1-15),in cluding one new compound(1),were separated and identified from g oji ber...Goji berry is a famous edible and medicinal substance around the world.In this research,15 phenylpropionyl phenylethylamine derivatives(1-15),in cluding one new compound(1),were separated and identified from g oji berry.All isolates were elucidated via ex tensive nuclear magnetic resonance spectral analyses and chemical techniques.Six known isolates were first obtained from Lycium genus.Six isolates were effectively split in to double chromatographic peaks accompanied by the basically identical areas,indicating they belong to racemates.The oxygen radical absorbance capacity(ORAC)experiment indicated that all isolates displayed a capacity of scavenging free radicals,and most of them exhibited higher ORAC than epigallocatechingallate.In ethanol/palmitic acid-established in vitro hepatocyte injury model,four phenylpropionyl phenylethylamine derivatives(1,2,9,and 15)significantly alleviated hepatocyte injury,among which compound 1 exerted the strongest protective activity.Notably,the hepatoprotective effect of compound 1 was further confirmed in ethanol-established liver damage mouse model,reflected by the reduction of lipid accumulation and the attenuation of pathological alteration.Combined with the in vitro results,the in vivo observations suggested that compound 1 suppressed cell apoptosis and the outburst of inflammation.Our findings provided first-hand evidence proving that goji berry-derived phenylpropionyl phenylethylamine derivatives hold a potential in treating alcoholic liver disease.展开更多
AIM： To evaluate the efficacy of Goji berry supplementation on improving macular pigment, serum zeaxanthin levels and visual acuity in patients with early age-related macular degeneration （AMD）. METHODS： A total ...AIM： To evaluate the efficacy of Goji berry supplementation on improving macular pigment, serum zeaxanthin levels and visual acuity in patients with early age-related macular degeneration （AMD）. METHODS： A total of 114 patients （aged from 51 to 92y, mean age 69.53±8.41y） with early AMD were enrolled in our prospective, randomized controlled study. The included patients were assigned randomly to the Goji group （n=57） with 25 g of Goji berries supplementation per day for 90d and the control group （n=57） with their normal diet for 90d. Macular pigment optical density （MPOD） was measured using heterochromatic flicker photometry （HFP）. The levels of serum lutein （L）/zeaxanthin （Z） were analyzed using high-performance liquid chromatography （HPLC）. MPOD, serum L/Z levels and best corrected visual acuity （BCVA） were recorded at baseline and 90d. RESULTS： In the Goji group, there were no statistically significant differences in the serum L levels between the baseline （0.199±0.149 μmol/mL） and 90d （0.203±0.181 μmol/mL） （t=-0.186, P=0.850）; however the serum Z levels were increased at 90d （0.101±0.087 μmol/mL） compared with those at the baseline （0.029±0.032 μmol/mL） （t=6.412, P〈0.001）. Patients treated with Goji berry for 90d showed an elevated MPOD （0.877±0.202 DU） from the baseline （0.731±0.205 DU） （t=-4.741, P=0.000）. In contrast to the control group, the serum Z levels and MPOD were higher in the Goji group at 90d （both P〈0.05）. At 90d, patients with Goji berry supplementation had a relative decrease in BCVA （0.21±0.18 logMAR） compared with the baseline （0.27±0.20） （t=2.397, P=0.020）. CONCLUSION： Overall, daily supplementation with Goji berry for 90d improves MPOD by increasing serum Z levels rather than serum L levels in early AMD patients. Goji berry may be an effective therapeutic intervention for preventing the progression of early AMD.展开更多
Goji berry(Lycium barbarum L.)is substantially dependent on nitrogen fertilizer application,which can signifi-cantly enhance fruit yield and Goji berry industrial development in Ningxia,China.This study aimed to analyz...Goji berry(Lycium barbarum L.)is substantially dependent on nitrogen fertilizer application,which can signifi-cantly enhance fruit yield and Goji berry industrial development in Ningxia,China.This study aimed to analyze the functions of differential nitrogen application rates including low(N1),medium(N2),and high(N3)levels in soil microbial community structure(bacterial and fungal)at 2 diverse soil depths(0-20,20-40 cm)through high-throughput sequencing technology by targeting 16S RNA gene and ITS1&ITS2 regions.All the observed physicochemical parameters exhibited significant improvement(p<0.05)with increased levels of nitrogen and the highest values for most parameters were observed at N2.However,pH decreased(p<0.05)gradually.The alpha and beta diversity analyses for bacterial and fungal communities’metagenome displayed more similarities than differences among all groups.The top bacterial and fungal phyla and genera suggested no obvious(p>0.05)differences among three group treatments(N1,N2,and N3).Furthermore,the functional enrichment analysis demonstrated significant(p<0.05)enrichment of quorum sensing,cysteine and methionine metabolism,and transcriptional machinery for bacterial communities,while various saprotrophic functional roles for fungal communities.Conclusively,moderately reducing the use of N-supplemented fertilizers is conducive to increasing soil nitrogen utilization rate,which can contribute to sustainable agriculture practices through improved soil quality,and microbial community structure and functions.展开更多
GB （goji berry） has bioactive components capable of reversing the metabolic syndrome. This work investigated systemic, biometric and metabolic parameters of male rats fed with standard diet （group CD） or high-carb...GB （goji berry） has bioactive components capable of reversing the metabolic syndrome. This work investigated systemic, biometric and metabolic parameters of male rats fed with standard diet （group CD） or high-carbohydrate diet （group HC）. At 90 days of age the HC group was subdivided： one was given vehicle solution （HCD） and the other was given GB extract （HCDGB）, for 60 days. The vehicle was also given to the CD group. At 150 days of age, glucose tolerance test, tissue collection, plasmatic determinations, lipid content, in situ perfusion and oxidative stress of the liver were carried out. The GB supplementation improved the parameters of the metabolic syndrome caused by the HC diet, including decreased body weight gain, adiposity index, dyslipidemia, hyperinsulinemia, NAFLD, liver oxidative stress and gluconeogenesis. Together with the diminished insulin resistance, these results indicate the GB extract is an important adjuvant in the treatment of the metabolic syndrome.展开更多
Black goji berry(Lycium ruthenicum Murr.)is well-known for its high nutritional and medicinal value,largely due to its rich content of naturally occurring anthocyanins,which exhibit various health-promoting bioactivit...Black goji berry(Lycium ruthenicum Murr.)is well-known for its high nutritional and medicinal value,largely due to its rich content of naturally occurring anthocyanins,which exhibit various health-promoting bioactivities.In this study,we established a paraquat-induced oxidative stress model in Caenorhabditis elegans and found that black goji berry anthocyanins(BGA)significantly extended lifespan and improved mobility.Additionally,BGA significantly increased catalase(CAT)activity and the GSH/GSSG ratio,while reducing oxidative damage markers,including ROS,malondialdehyde(MDA),and protein carbonyl levels,demonstrating potent antioxidative stress activity in vivo.Furthermore,BGA improved mitochondrial morphology,partially restored damaged mitochondrial membrane potential(MMP)and ATP levels,and elevated relative mitochondrial DNA level under oxidative stress.By analyzing pyruvate,lactate,and TCA cycle metabolites,we hypothesize that BGA may enhance energy metabolism,with its mitochondrial protective effects mainly attributed to its petunidin derivatives,particularly petunidin-3-O-glucoside.Gene expression analysis revealed that BGA upregulated key electron transport chain genes,including cco-1,atp-2,and clk-1,potentially enhancing energy metabolism.Further investigation of lifespan,ROS levels,and gene expression in clk-1 mutants confirmed the critical role of clk-1 in BGA-mediated mitochondrial protection and oxidative stress resistance.Our findings provide new insights into the molecular pathways through which BGA exerts its protective effects against oxidative stress in vivo,supporting the potential use of black goji berry and its anthocyanins as functional foods and nutrients with anti-oxidative stress and mitochondrial-protective properties.展开更多
The market potential of fermented goji berry wine is constrained by its inherent lack of acidity and aroma.Lachancea thermotolerans was used to produce lactic acid,and together with Saccharomyces cerevisiae were used ...The market potential of fermented goji berry wine is constrained by its inherent lack of acidity and aroma.Lachancea thermotolerans was used to produce lactic acid,and together with Saccharomyces cerevisiae were used to ferment(pure or mixed)two varieties of goji berries.The physicochemical parameters,sensory profile(GDA),volatile compounds(GC-Orbitrap-MS)and non-volatile compounds(UPLC-QqQ-MS/MS)of the wines were analyzed.Fermentation with L.thermotolerans significantly increased the wine’s acidity,producing 11.97 g/L of lactic acid.L.thermotolerans also enhanced the“fruity”,“honey”,and“goji berry”aroma,and reduced the“animal”aroma to low level.Chemical analysis showed a general reduction in esters,while key volatile phenols associated with off-flavors,such as 4-ethylguaiacol,were reduced by up to 50%.Moreover,inoculation with L.thermotolerans resulted in an increase in free-state flavonoids(such as morin and quercetin),phenolic acids,and phenolamines in goji berry wine.Taken together,L.thermotolerans could be an effective tool to enhance the quality of goji berry wine and our study provided new insights into the fruit wine industry.展开更多
The physiological indices,reactive oxygen species(ROS)metabolism,and mitochondrial redox metabolism were investigated during 15 days of storage to assess the impact of 1-methylcyclopropene(1-MCP)on the postharvest qua...The physiological indices,reactive oxygen species(ROS)metabolism,and mitochondrial redox metabolism were investigated during 15 days of storage to assess the impact of 1-methylcyclopropene(1-MCP)on the postharvest quality of goji berries in both control and treated groups.The results demonstrated that 1-MCP maintained the physiological qualities of postharvest goji berry fruits,including delaying the appearance of respiratory peak,increasing titratable acid content,and decreasing weight loss.Transcriptome analysis indicated that 1-MCP activated the transcription of genes related to ROS and mitochondrial redox metabolism in goji berry fruits.1-MCP suppressed the increase in conductivity and membrane lipid peroxidation,and reduced the generation rate of O_(2)^(·-)(112.7 mmol min^(-1) g^(-1))and the content of H_(2)O_(2)(0.75μmol g^(-1)).Meanwhile,1-MCP activated the activities of superoxide dismutase(SOD)(2.4 U g^(-1)),catalase(CAT)(46.4 U g^(-1)),peroxidase(POD)(3.6 U g^(-1)),ascorbate peroxidase(APX)(1.9 U g^(-1))and glutathione reductase(GR).Besides,the amount of ascorbic acid(AsA)and glutathione(GSH)was promoted by 1-MCP,while the opposite was noted in the glutathione disulfide(GSSG)content.1-MCP treatment enhanced the NAD(P)+content,decreased the NAD(P)H content,and increased the NAD(P)+/NAD(P)H ratio in mitochondrial.Results suggest that postharvest storage quality of goji berry could be promoted by 1-MCP via regulation of ROS metabolism and mitochondrial redox metabolism.展开更多
Subthreshold depression is a highly prevalent condition in adolescents who are at high risk for developing major depressive disorder.In preclinical models of neurological and psychiatric diseases,Lycium barbarum polys...Subthreshold depression is a highly prevalent condition in adolescents who are at high risk for developing major depressive disorder.In preclinical models of neurological and psychiatric diseases,Lycium barbarum polysaccharide(LBP)extracted from Goji berries had antidepressant effects including but not limited to anti-oxidative and anti-inflammatory properties.However,the effect of LBP on subthreshold depression is unclear.To investigate the clinical efficacy and safety of LBP for treating subthreshold depression in adolescents,we conducted a randomized,double-blind,placebo-controlled trial(RCT)with 29 adolescents with subthreshold depression recruited at The Fifth Affiliated Hospital of Guangzhou Medical University.The participants were randomly assigned to groups where they received either 300 mg LBP(LBP group,n=15,3 boys and 12 girls aged 15.13±2.17 years)or a placebo(placebo group,n=14,2 boys and 12 girls aged 15±1.71 years)for 6 successive weeks.Interim analyses revealed that the LBP group exhibited a greater change in Hamilton Depression Scale(HAMD-24)scores relative to the baseline and a higher remission rate(HAMD-24 total score≤7)at 6 weeks compared with the placebo group.Scores on the Beck Depression Inventory-II(BDI-II),Pittsburgh Sleep Quality Index(PSQI),Kessler Psychological Distress Scale(Kessler),and Screen for Child Anxiety-Related Emotional Disorders(SCARED)were similar between the LBP and placebo groups.No side effects related to the intervention were reported in either group.These results indicate that LBP administration reduced depressive symptoms in adolescents with subthreshold depression.Furthermore,LBP was well tolerated with no treatment-limiting adverse events.Clinical trials involving a larger sample size are needed to further confirm the anti-depressive effects of LBP in adolescents with subthreshold depression.This study was approved by the Medical Ethics Committee of the Fifth Affiliated Hospital of Guangzhou Medical University(Guangzhou,China;approval No.L2019-08)on April 4,2019 and was registered on ClinicalTrials.gov(identifier:NCT04032795)on July 25,2019.展开更多
Strong evidence has accumulated to show a correlation between depression symptoms and inflammatory responses.Moreover,anti-inflammatory treatment has shown partial effectiveness in alleviating depression symptoms.Lyci...Strong evidence has accumulated to show a correlation between depression symptoms and inflammatory responses.Moreover,anti-inflammatory treatment has shown partial effectiveness in alleviating depression symptoms.Lycium barbarum polysaccharide(LBP),derived from Goji berries,exhibits notable antioxidative and anti-inflammatory properties.In our recent double-blinded randomized placebo-controlled trial,we found that LBP significantly reduced depressive symptoms in adolescents with subthreshold depression.It is presumed that the antidepressant effect of LBP may be associated with its influence on inflammatory cytokines.In the double-blinded randomized controlled trial,we enrolled 29 adolescents with subthreshold depression and randomly divided them into an LBP group and a placebo group.In the LBP group,adolescents were given 300 mg/d LBP.A 6-week follow up was completed by 24 adolescents,comprising 14 adolescents from the LBP group(15.36±2.06 years,3 men and 11 women)and 10 adolescents from the placebo group(14.9±1.6 years,2 men and 8 women).Our results showed that after 6 weeks of treatment,the interleukin-17A level in the LBP group was lower than that in the placebo group.Network analysis showed that LBP reduced the correlations and connectivity between inflammatory factors,which were associated with the improvement in depressive symptoms.These findings suggest that 6-week administration of LBP suppresses the immune response by reducing interleukin-17A level,thereby exerting an antidepressant effect.展开更多
Nurtured by the River Chief Director:Wang Doudou Length:One Episode Producers:Publicity Department of the CPC Committee of Ningxia Hui Autonomous Region,China Global Television Network,Forestry and Grassland Bureau of...Nurtured by the River Chief Director:Wang Doudou Length:One Episode Producers:Publicity Department of the CPC Committee of Ningxia Hui Autonomous Region,China Global Television Network,Forestry and Grassland Bureau of Ningxia Hui Autonomous Region Broadcasting Platform:bilibili Northwest China’s Ningxia Hui Autonomous Region has long been the homeland and a key production area for goji berries in China.This film focuses on the national strategy of ecological protection and high-quality development in the Yellow River Basin,showcasing Ningxia’s transformation from traditional agriculture to modern specialty industries.It presents viewers with the supply chain of goji berries in Ningxia and takes them on a journey through the stories of local residents’relentless pursuit and deep affection for advancing the industry,portraying a vibrant picture of its thriving future architecture,and artifacts.展开更多
Goji berry tea, a traditional herbal tea, is the main ate mode of goji berry in Asia, yet few studies in comparison with red goji berry tea and black goji berry tea are carried out. This study investigated the effects...Goji berry tea, a traditional herbal tea, is the main ate mode of goji berry in Asia, yet few studies in comparison with red goji berry tea and black goji berry tea are carried out. This study investigated the effects of water temperature and soak time on the colour, phytochemicals, and the antioxidant capacity [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and the ferric-reducing antioxidant power (FRAP)] of two goji berry tea. A comparison of the bioactive compounds and antioxidant activities between black and red goji berry tea was conducted. Results showed that both red and black goji berry tea were rich in phytochemicals, giving high antioxidant ability. The levels of bioactive compounds and the antioxidant activity of the two goji berry tea increased as the increases in soak temperature and time. Black goji berry tea had higher phytochemicals and antioxidant property than those of red goji berry tea. Infused at 100° water for the same time, the levels of total polysaccharides (150 mg/100 ml), total polyphenols (238 mg/ml), and antioxidant capacity (550 μmol/100 ml) of black goji berry tea were 3.5, 2, and 5 times higher, respectively, in comparison with red goji berry tea. The results of this study demonstrate that hot drink of goji berry in China is a good habit and black goji berry tea may be a better choice.展开更多
This study describes the changes in the microbial growth,physicochemical properties,phenolics,enzyme activity,and antioxidant activity of Goji berry juice during fermentation with Tibetan kefir grains(TKG).The results...This study describes the changes in the microbial growth,physicochemical properties,phenolics,enzyme activity,and antioxidant activity of Goji berry juice during fermentation with Tibetan kefir grains(TKG).The results showed that TKG grew well in Goji berry juice,and yeasts were dominant in the later period of fermentation(5.20×10^(8)CFU/mL).The main fungal genus of TKG and fermented Goji berry juice(24,48,and 72 h)was Kazachstania stably.The relative abundance of Lactobacillus in Goji berry juice decreased from 92.05%to 33.11%after 72 h-fermentation,while that of Acetobacter increased to 66.81%.In addition,72 h-fermentation led to the consumption of 82.55%of total sugar and 93.90%of reducing sugar,as well as dramatic changes in organic acids.The main volatile substances changed from aldehydes(61.98%)to alcohols(55.24%)and esters(38.54%).The content of total soluble flavonoids(STFC)in Goji berry juice was significantly increased from 42.28 mg RE/100 mL to 85.69 mg RE/100 mL after complete fermentation(p<0.01).Furthermore,16 flavonoids,eight phenolic acids,one phenolic acid derivative,and two coumarins were identified by HRLC-ESI-MS/MS.α-Amylase and xylanase were primarily responsible for the release of flavonoids(mangiferin,rutin,hyperoside,isoquercitrin,and quercetin)during fermentation,thus significantly enhancing the reducing power and OH⋅scavenging activity of Goji berry juice.展开更多
The aim of this study was to optimize the conditions for combined ultrasonication and enzymatic treatment of whole fruit Lycium barbarum.The synergistic effects of ultrasonication and enzymatic treatment on the fermen...The aim of this study was to optimize the conditions for combined ultrasonication and enzymatic treatment of whole fruit Lycium barbarum.The synergistic effects of ultrasonication and enzymatic treatment on the fermentation process,bioactive compounds,and antioxidant activity were investigated.Optimal combination of sonication time,enzyme ratio(cellulase:pectinase:protease),and enzyme concentration was determined by optimization experiments to be 10 min,1:1:1,and 0.75%,respectively.Under these conditions,the fermentation process greatly accelerated,the viable cell count of the lactic acid bacteria after fermentation for 36 h reached(5.57±0.38)×10^(8)CFU g^(-1)compared with(8.63±0.29)×10^(7)CFU g^(-1)for samples without pretreatment.The total phenol and flavonoid contents of Lycium barbarum with pretreatment after fermentation for 36 h increased by 10.69%and 19.62%,respectively,compared with samples without pretreatment.The highest antioxidant activities,as measured by a 2,2-diphenyl-1-picrylhydrazyl(DPPH)assay,hydroxyl radical scavenging assay,and superoxide anion scavenging assay,were obtained for the treated Lycium barbarum.展开更多
Hair loss is the dysfunction and depletion of hair progenitor cells,leading to hair follicle miniaturization.Oxidative stress and inflammatory responses contribute to hair follicle death,causing hair loss.This study a...Hair loss is the dysfunction and depletion of hair progenitor cells,leading to hair follicle miniaturization.Oxidative stress and inflammatory responses contribute to hair follicle death,causing hair loss.This study aimed to evaluate the hair growth-promoting capacities of Limosilactobacillus fermentum MF10(MF10)and MF10-fermented Goji berries in a depilation mouse model.Both treatments were selected because their anti-oxidative and anti-inflammatory properties have been confirmed in vitro.Black coloration in the shaved skin of mice was apparent from day 12 and was the highest in the MF10-treated group.Until day 19,the highest pigmentation was observed in the MF10-treated group,not significantly different from that observed in the fi-nasteride(Fin)-treated group,the positive control.Notably,hair follicle size increased only by the MF10 treatment.Skin Wnt/β-catenin signaling was activated by MF10 through downregulation of p-GSK3B protein expression and accumulation ofβ-catenin,leading increased serum vascular endothelial growth factor levels only in the MF10 group.Additionally,only MF10 modulated the gut microbial abundance of Duncaniella,which correlated with p-GSK3B protein expression.Overall,our results indicate that L.fermentum MF10-treatment promotes hair growth,and that these effects may be due to gut microbiota activating the skin Wnt/β-catenin signaling.展开更多
Objective To evaluate the variation of sweet chemicals（sugars and betaine） in fruits of six species in genus Lycium L. i.e. Lycium truncatum, L. cylindricum, L. dasystemum, L. dasystemum var. rubricaulium, L. chinen...Objective To evaluate the variation of sweet chemicals（sugars and betaine） in fruits of six species in genus Lycium L. i.e. Lycium truncatum, L. cylindricum, L. dasystemum, L. dasystemum var. rubricaulium, L. chinense, and L. barbarum harvested at three different ripening stages. Methods A simple and effective method based on UPLC-ELSD was developed for the simultaneous determination of two monosaccharides（glucose and fructose）, two disaccharides（sucrose and maltose）, one sugar alcohol（xylitol）, and betaine in wolfberry fruits（goji berries） of genus Lycium L. Results The six species of wolfberry fruits harvested at the three different ripening stages were evaluated in sugars and betaine contents. Fructose and glucose were the predominant sugars in mature wolfberry fruits. Fructose, glucose, and betaine, as well as total sugar contents, increased continually over the ripening process. L. truncatum and L. dasystemum had higher contents of sugars and betaine than the other species. Conclusion UPLC-ELSD is a simple, reliable and effective method for analysis of the sweet chemicals in wolfberries. Wolfberry fruits at the different ripening stages were significant different in sweetness. L. truncatum and L. dasystemum could be the potential sources of wolfberry fruit sources.展开更多
基金supported by the National Key research and Development Program of china(2023YFF1103802).
文摘The eye is one of the important organs for human beings to obtain information from the outside world and avoid harm,and 90%of human knowledge is obtained through the eye.The center of the retina in the human eye is called the macula,and it is the area where vision is most concentrated.Current research suggests that macular pigment is mainly composed of lutein,zeaxanthin,and meso-zeaxanthin,which are able to absorb and attenuate blue light damage to the retina,and since humans cannot synthesize these carotenoids,they must be obtained by supplementing with carotenoid-rich foods.In addition,carotenoids also provide good provitamin A,which provides necessary nutritional support for people’s visual transduction.Therefore,eye health is inseparable from carotenoid supplementation,among which wolfberry,which is rich in a variety of carotenoids,has been confirmed by recent studies to have a good role in promoting eye health,and also provides a good source of natural carotenoids for humans.However,a systematic review of goji berry carotenoids in eye health is lacking in current studies.Based on this,this paper reviews the main carotenoid composition and its extraction and purification in wolfberry,discusses the changes of carotenoids in the process of harvesting and drying wolfberry,reviews the absorption and utilization of carotenoids in wolfberry,and more importantly,discusses the important role of carotenoids in wolfberry in eye health.
基金funded via National Natural Science Foundation of China(81925037,82321004,U22A20371,and 82273801)Guangdong Major Project of Basic and Applied Basic Research(2023B0303000026)+6 种基金Guangdong International Science and Technology Cooperation Base(2021A0505020015)Innovative and Research Teams Project of Guangdong Higher Education Institution(2021KCXTD001)Guangdong Basic and Applied Basic Research Foundation(2022A1515012071)Young Elite Scientists Sponsorship Program by China Association of Chinese Medicine(2021-QNRC2-B21)Guangdong-Hong Kong-Macao Universities Joint Laboratory for the Internationalization of Traditional Chinese Medicine(2023LSYS002)Guangzhou Key Laboratory of Traditional Chinese Medicine&Disease Susceptibility(2024A03J090)Science and Technology Program of Guangzhou(201707010414,202201020066,and 2024A04J9955)。
文摘Goji berry is a famous edible and medicinal substance around the world.In this research,15 phenylpropionyl phenylethylamine derivatives(1-15),in cluding one new compound(1),were separated and identified from g oji berry.All isolates were elucidated via ex tensive nuclear magnetic resonance spectral analyses and chemical techniques.Six known isolates were first obtained from Lycium genus.Six isolates were effectively split in to double chromatographic peaks accompanied by the basically identical areas,indicating they belong to racemates.The oxygen radical absorbance capacity(ORAC)experiment indicated that all isolates displayed a capacity of scavenging free radicals,and most of them exhibited higher ORAC than epigallocatechingallate.In ethanol/palmitic acid-established in vitro hepatocyte injury model,four phenylpropionyl phenylethylamine derivatives(1,2,9,and 15)significantly alleviated hepatocyte injury,among which compound 1 exerted the strongest protective activity.Notably,the hepatoprotective effect of compound 1 was further confirmed in ethanol-established liver damage mouse model,reflected by the reduction of lipid accumulation and the attenuation of pathological alteration.Combined with the in vitro results,the in vivo observations suggested that compound 1 suppressed cell apoptosis and the outburst of inflammation.Our findings provided first-hand evidence proving that goji berry-derived phenylpropionyl phenylethylamine derivatives hold a potential in treating alcoholic liver disease.
基金Supported by Special Foundation for Public Welfare Research of China(No.2013CZ-9)
文摘AIM： To evaluate the efficacy of Goji berry supplementation on improving macular pigment, serum zeaxanthin levels and visual acuity in patients with early age-related macular degeneration （AMD）. METHODS： A total of 114 patients （aged from 51 to 92y, mean age 69.53±8.41y） with early AMD were enrolled in our prospective, randomized controlled study. The included patients were assigned randomly to the Goji group （n=57） with 25 g of Goji berries supplementation per day for 90d and the control group （n=57） with their normal diet for 90d. Macular pigment optical density （MPOD） was measured using heterochromatic flicker photometry （HFP）. The levels of serum lutein （L）/zeaxanthin （Z） were analyzed using high-performance liquid chromatography （HPLC）. MPOD, serum L/Z levels and best corrected visual acuity （BCVA） were recorded at baseline and 90d. RESULTS： In the Goji group, there were no statistically significant differences in the serum L levels between the baseline （0.199±0.149 μmol/mL） and 90d （0.203±0.181 μmol/mL） （t=-0.186, P=0.850）; however the serum Z levels were increased at 90d （0.101±0.087 μmol/mL） compared with those at the baseline （0.029±0.032 μmol/mL） （t=6.412, P〈0.001）. Patients treated with Goji berry for 90d showed an elevated MPOD （0.877±0.202 DU） from the baseline （0.731±0.205 DU） （t=-4.741, P=0.000）. In contrast to the control group, the serum Z levels and MPOD were higher in the Goji group at 90d （both P〈0.05）. At 90d, patients with Goji berry supplementation had a relative decrease in BCVA （0.21±0.18 logMAR） compared with the baseline （0.27±0.20） （t=2.397, P=0.020）. CONCLUSION： Overall, daily supplementation with Goji berry for 90d improves MPOD by increasing serum Z levels rather than serum L levels in early AMD patients. Goji berry may be an effective therapeutic intervention for preventing the progression of early AMD.
基金This work was funded by Ningxia Hui Autonomous Region Key Research and Development Project(2021BEF02004),Central Finance Forestry Reform and Development Fund“Forest Seed Cultivation”.
文摘Goji berry(Lycium barbarum L.)is substantially dependent on nitrogen fertilizer application,which can signifi-cantly enhance fruit yield and Goji berry industrial development in Ningxia,China.This study aimed to analyze the functions of differential nitrogen application rates including low(N1),medium(N2),and high(N3)levels in soil microbial community structure(bacterial and fungal)at 2 diverse soil depths(0-20,20-40 cm)through high-throughput sequencing technology by targeting 16S RNA gene and ITS1&ITS2 regions.All the observed physicochemical parameters exhibited significant improvement(p<0.05)with increased levels of nitrogen and the highest values for most parameters were observed at N2.However,pH decreased(p<0.05)gradually.The alpha and beta diversity analyses for bacterial and fungal communities’metagenome displayed more similarities than differences among all groups.The top bacterial and fungal phyla and genera suggested no obvious(p>0.05)differences among three group treatments(N1,N2,and N3).Furthermore,the functional enrichment analysis demonstrated significant(p<0.05)enrichment of quorum sensing,cysteine and methionine metabolism,and transcriptional machinery for bacterial communities,while various saprotrophic functional roles for fungal communities.Conclusively,moderately reducing the use of N-supplemented fertilizers is conducive to increasing soil nitrogen utilization rate,which can contribute to sustainable agriculture practices through improved soil quality,and microbial community structure and functions.
文摘GB （goji berry） has bioactive components capable of reversing the metabolic syndrome. This work investigated systemic, biometric and metabolic parameters of male rats fed with standard diet （group CD） or high-carbohydrate diet （group HC）. At 90 days of age the HC group was subdivided： one was given vehicle solution （HCD） and the other was given GB extract （HCDGB）, for 60 days. The vehicle was also given to the CD group. At 150 days of age, glucose tolerance test, tissue collection, plasmatic determinations, lipid content, in situ perfusion and oxidative stress of the liver were carried out. The GB supplementation improved the parameters of the metabolic syndrome caused by the HC diet, including decreased body weight gain, adiposity index, dyslipidemia, hyperinsulinemia, NAFLD, liver oxidative stress and gluconeogenesis. Together with the diminished insulin resistance, these results indicate the GB extract is an important adjuvant in the treatment of the metabolic syndrome.
基金supported by the 111 Project(B17018)supported by Dasong Agricultural Technology(2020-8950).
文摘Black goji berry(Lycium ruthenicum Murr.)is well-known for its high nutritional and medicinal value,largely due to its rich content of naturally occurring anthocyanins,which exhibit various health-promoting bioactivities.In this study,we established a paraquat-induced oxidative stress model in Caenorhabditis elegans and found that black goji berry anthocyanins(BGA)significantly extended lifespan and improved mobility.Additionally,BGA significantly increased catalase(CAT)activity and the GSH/GSSG ratio,while reducing oxidative damage markers,including ROS,malondialdehyde(MDA),and protein carbonyl levels,demonstrating potent antioxidative stress activity in vivo.Furthermore,BGA improved mitochondrial morphology,partially restored damaged mitochondrial membrane potential(MMP)and ATP levels,and elevated relative mitochondrial DNA level under oxidative stress.By analyzing pyruvate,lactate,and TCA cycle metabolites,we hypothesize that BGA may enhance energy metabolism,with its mitochondrial protective effects mainly attributed to its petunidin derivatives,particularly petunidin-3-O-glucoside.Gene expression analysis revealed that BGA upregulated key electron transport chain genes,including cco-1,atp-2,and clk-1,potentially enhancing energy metabolism.Further investigation of lifespan,ROS levels,and gene expression in clk-1 mutants confirmed the critical role of clk-1 in BGA-mediated mitochondrial protection and oxidative stress resistance.Our findings provide new insights into the molecular pathways through which BGA exerts its protective effects against oxidative stress in vivo,supporting the potential use of black goji berry and its anthocyanins as functional foods and nutrients with anti-oxidative stress and mitochondrial-protective properties.
基金supported by the Key R&D projects in China People’s Police University,China(ZDX202101)Fundamental Research Funds for the Central Universities,China(2021ZY65)the Beijing Engineering and Technology Research Center of Food Additives,China(2019GSDX001).
文摘The market potential of fermented goji berry wine is constrained by its inherent lack of acidity and aroma.Lachancea thermotolerans was used to produce lactic acid,and together with Saccharomyces cerevisiae were used to ferment(pure or mixed)two varieties of goji berries.The physicochemical parameters,sensory profile(GDA),volatile compounds(GC-Orbitrap-MS)and non-volatile compounds(UPLC-QqQ-MS/MS)of the wines were analyzed.Fermentation with L.thermotolerans significantly increased the wine’s acidity,producing 11.97 g/L of lactic acid.L.thermotolerans also enhanced the“fruity”,“honey”,and“goji berry”aroma,and reduced the“animal”aroma to low level.Chemical analysis showed a general reduction in esters,while key volatile phenols associated with off-flavors,such as 4-ethylguaiacol,were reduced by up to 50%.Moreover,inoculation with L.thermotolerans resulted in an increase in free-state flavonoids(such as morin and quercetin),phenolic acids,and phenolamines in goji berry wine.Taken together,L.thermotolerans could be an effective tool to enhance the quality of goji berry wine and our study provided new insights into the fruit wine industry.
基金financially supported by the National Natural Science Foundation of China(32302175).
文摘The physiological indices,reactive oxygen species(ROS)metabolism,and mitochondrial redox metabolism were investigated during 15 days of storage to assess the impact of 1-methylcyclopropene(1-MCP)on the postharvest quality of goji berries in both control and treated groups.The results demonstrated that 1-MCP maintained the physiological qualities of postharvest goji berry fruits,including delaying the appearance of respiratory peak,increasing titratable acid content,and decreasing weight loss.Transcriptome analysis indicated that 1-MCP activated the transcription of genes related to ROS and mitochondrial redox metabolism in goji berry fruits.1-MCP suppressed the increase in conductivity and membrane lipid peroxidation,and reduced the generation rate of O_(2)^(·-)(112.7 mmol min^(-1) g^(-1))and the content of H_(2)O_(2)(0.75μmol g^(-1)).Meanwhile,1-MCP activated the activities of superoxide dismutase(SOD)(2.4 U g^(-1)),catalase(CAT)(46.4 U g^(-1)),peroxidase(POD)(3.6 U g^(-1)),ascorbate peroxidase(APX)(1.9 U g^(-1))and glutathione reductase(GR).Besides,the amount of ascorbic acid(AsA)and glutathione(GSH)was promoted by 1-MCP,while the opposite was noted in the glutathione disulfide(GSSG)content.1-MCP treatment enhanced the NAD(P)+content,decreased the NAD(P)H content,and increased the NAD(P)+/NAD(P)H ratio in mitochondrial.Results suggest that postharvest storage quality of goji berry could be promoted by 1-MCP via regulation of ROS metabolism and mitochondrial redox metabolism.
基金financially supported by the National Natural Science Foundation of China,No.81671347(to KL)The Science and Technology Program of Guangzhou,China,No.202007030012(to KFS and KL)。
文摘Subthreshold depression is a highly prevalent condition in adolescents who are at high risk for developing major depressive disorder.In preclinical models of neurological and psychiatric diseases,Lycium barbarum polysaccharide(LBP)extracted from Goji berries had antidepressant effects including but not limited to anti-oxidative and anti-inflammatory properties.However,the effect of LBP on subthreshold depression is unclear.To investigate the clinical efficacy and safety of LBP for treating subthreshold depression in adolescents,we conducted a randomized,double-blind,placebo-controlled trial(RCT)with 29 adolescents with subthreshold depression recruited at The Fifth Affiliated Hospital of Guangzhou Medical University.The participants were randomly assigned to groups where they received either 300 mg LBP(LBP group,n=15,3 boys and 12 girls aged 15.13±2.17 years)or a placebo(placebo group,n=14,2 boys and 12 girls aged 15±1.71 years)for 6 successive weeks.Interim analyses revealed that the LBP group exhibited a greater change in Hamilton Depression Scale(HAMD-24)scores relative to the baseline and a higher remission rate(HAMD-24 total score≤7)at 6 weeks compared with the placebo group.Scores on the Beck Depression Inventory-II(BDI-II),Pittsburgh Sleep Quality Index(PSQI),Kessler Psychological Distress Scale(Kessler),and Screen for Child Anxiety-Related Emotional Disorders(SCARED)were similar between the LBP and placebo groups.No side effects related to the intervention were reported in either group.These results indicate that LBP administration reduced depressive symptoms in adolescents with subthreshold depression.Furthermore,LBP was well tolerated with no treatment-limiting adverse events.Clinical trials involving a larger sample size are needed to further confirm the anti-depressive effects of LBP in adolescents with subthreshold depression.This study was approved by the Medical Ethics Committee of the Fifth Affiliated Hospital of Guangzhou Medical University(Guangzhou,China;approval No.L2019-08)on April 4,2019 and was registered on ClinicalTrials.gov(identifier:NCT04032795)on July 25,2019.
基金supported by the National Natural Science Foundation of China,No.81671347(to KL)the Science and Technology Program of Guangzhou of China,No.202007030012(to KFS and KL)the Science and Technology Program of Guangzhou of China,No 202102020735(to RW).
文摘Strong evidence has accumulated to show a correlation between depression symptoms and inflammatory responses.Moreover,anti-inflammatory treatment has shown partial effectiveness in alleviating depression symptoms.Lycium barbarum polysaccharide(LBP),derived from Goji berries,exhibits notable antioxidative and anti-inflammatory properties.In our recent double-blinded randomized placebo-controlled trial,we found that LBP significantly reduced depressive symptoms in adolescents with subthreshold depression.It is presumed that the antidepressant effect of LBP may be associated with its influence on inflammatory cytokines.In the double-blinded randomized controlled trial,we enrolled 29 adolescents with subthreshold depression and randomly divided them into an LBP group and a placebo group.In the LBP group,adolescents were given 300 mg/d LBP.A 6-week follow up was completed by 24 adolescents,comprising 14 adolescents from the LBP group(15.36±2.06 years,3 men and 11 women)and 10 adolescents from the placebo group(14.9±1.6 years,2 men and 8 women).Our results showed that after 6 weeks of treatment,the interleukin-17A level in the LBP group was lower than that in the placebo group.Network analysis showed that LBP reduced the correlations and connectivity between inflammatory factors,which were associated with the improvement in depressive symptoms.These findings suggest that 6-week administration of LBP suppresses the immune response by reducing interleukin-17A level,thereby exerting an antidepressant effect.
文摘Nurtured by the River Chief Director:Wang Doudou Length:One Episode Producers:Publicity Department of the CPC Committee of Ningxia Hui Autonomous Region,China Global Television Network,Forestry and Grassland Bureau of Ningxia Hui Autonomous Region Broadcasting Platform:bilibili Northwest China’s Ningxia Hui Autonomous Region has long been the homeland and a key production area for goji berries in China.This film focuses on the national strategy of ecological protection and high-quality development in the Yellow River Basin,showcasing Ningxia’s transformation from traditional agriculture to modern specialty industries.It presents viewers with the supply chain of goji berries in Ningxia and takes them on a journey through the stories of local residents’relentless pursuit and deep affection for advancing the industry,portraying a vibrant picture of its thriving future architecture,and artifacts.
基金This work was financially supported by National Natural Science Foundation of China(31771982 and 31401474).
文摘Goji berry tea, a traditional herbal tea, is the main ate mode of goji berry in Asia, yet few studies in comparison with red goji berry tea and black goji berry tea are carried out. This study investigated the effects of water temperature and soak time on the colour, phytochemicals, and the antioxidant capacity [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and the ferric-reducing antioxidant power (FRAP)] of two goji berry tea. A comparison of the bioactive compounds and antioxidant activities between black and red goji berry tea was conducted. Results showed that both red and black goji berry tea were rich in phytochemicals, giving high antioxidant ability. The levels of bioactive compounds and the antioxidant activity of the two goji berry tea increased as the increases in soak temperature and time. Black goji berry tea had higher phytochemicals and antioxidant property than those of red goji berry tea. Infused at 100° water for the same time, the levels of total polysaccharides (150 mg/100 ml), total polyphenols (238 mg/ml), and antioxidant capacity (550 μmol/100 ml) of black goji berry tea were 3.5, 2, and 5 times higher, respectively, in comparison with red goji berry tea. The results of this study demonstrate that hot drink of goji berry in China is a good habit and black goji berry tea may be a better choice.
基金supported by the national 13th Five-Year key Research and Development Plan project(grant number 2019YFC1606703).
文摘This study describes the changes in the microbial growth,physicochemical properties,phenolics,enzyme activity,and antioxidant activity of Goji berry juice during fermentation with Tibetan kefir grains(TKG).The results showed that TKG grew well in Goji berry juice,and yeasts were dominant in the later period of fermentation(5.20×10^(8)CFU/mL).The main fungal genus of TKG and fermented Goji berry juice(24,48,and 72 h)was Kazachstania stably.The relative abundance of Lactobacillus in Goji berry juice decreased from 92.05%to 33.11%after 72 h-fermentation,while that of Acetobacter increased to 66.81%.In addition,72 h-fermentation led to the consumption of 82.55%of total sugar and 93.90%of reducing sugar,as well as dramatic changes in organic acids.The main volatile substances changed from aldehydes(61.98%)to alcohols(55.24%)and esters(38.54%).The content of total soluble flavonoids(STFC)in Goji berry juice was significantly increased from 42.28 mg RE/100 mL to 85.69 mg RE/100 mL after complete fermentation(p<0.01).Furthermore,16 flavonoids,eight phenolic acids,one phenolic acid derivative,and two coumarins were identified by HRLC-ESI-MS/MS.α-Amylase and xylanase were primarily responsible for the release of flavonoids(mangiferin,rutin,hyperoside,isoquercitrin,and quercetin)during fermentation,thus significantly enhancing the reducing power and OH⋅scavenging activity of Goji berry juice.
基金funded by the Tianjin Science and Technology Support Key Project(20YFZCSN00980)the Innovative Team of Modern Agricultural Industry Technology System in Tianjin(ITTHRS2021000).
文摘The aim of this study was to optimize the conditions for combined ultrasonication and enzymatic treatment of whole fruit Lycium barbarum.The synergistic effects of ultrasonication and enzymatic treatment on the fermentation process,bioactive compounds,and antioxidant activity were investigated.Optimal combination of sonication time,enzyme ratio(cellulase:pectinase:protease),and enzyme concentration was determined by optimization experiments to be 10 min,1:1:1,and 0.75%,respectively.Under these conditions,the fermentation process greatly accelerated,the viable cell count of the lactic acid bacteria after fermentation for 36 h reached(5.57±0.38)×10^(8)CFU g^(-1)compared with(8.63±0.29)×10^(7)CFU g^(-1)for samples without pretreatment.The total phenol and flavonoid contents of Lycium barbarum with pretreatment after fermentation for 36 h increased by 10.69%and 19.62%,respectively,compared with samples without pretreatment.The highest antioxidant activities,as measured by a 2,2-diphenyl-1-picrylhydrazyl(DPPH)assay,hydroxyl radical scavenging assay,and superoxide anion scavenging assay,were obtained for the treated Lycium barbarum.
基金supported by Korea University,TOV Co.Ltd,High Value-Added Food Technology Development Program of the Korea Institute of Planning and Evaluation for Technology in Food,Agriculture,Forestry,and Fisheries,and the Ministry for Food,Agriculture,Forestry,and Fisheries of the Republic of Korea[Grant numbers 321036053SB010,1545027002].
文摘Hair loss is the dysfunction and depletion of hair progenitor cells,leading to hair follicle miniaturization.Oxidative stress and inflammatory responses contribute to hair follicle death,causing hair loss.This study aimed to evaluate the hair growth-promoting capacities of Limosilactobacillus fermentum MF10(MF10)and MF10-fermented Goji berries in a depilation mouse model.Both treatments were selected because their anti-oxidative and anti-inflammatory properties have been confirmed in vitro.Black coloration in the shaved skin of mice was apparent from day 12 and was the highest in the MF10-treated group.Until day 19,the highest pigmentation was observed in the MF10-treated group,not significantly different from that observed in the fi-nasteride(Fin)-treated group,the positive control.Notably,hair follicle size increased only by the MF10 treatment.Skin Wnt/β-catenin signaling was activated by MF10 through downregulation of p-GSK3B protein expression and accumulation ofβ-catenin,leading increased serum vascular endothelial growth factor levels only in the MF10 group.Additionally,only MF10 modulated the gut microbial abundance of Duncaniella,which correlated with p-GSK3B protein expression.Overall,our results indicate that L.fermentum MF10-treatment promotes hair growth,and that these effects may be due to gut microbiota activating the skin Wnt/β-catenin signaling.
基金National Science Fund for Distinguished Young Scholars of China(No.81325023)Basic Scientific Research Fund from Ministry of Finance of China(No.zz2016006)
文摘Objective To evaluate the variation of sweet chemicals（sugars and betaine） in fruits of six species in genus Lycium L. i.e. Lycium truncatum, L. cylindricum, L. dasystemum, L. dasystemum var. rubricaulium, L. chinense, and L. barbarum harvested at three different ripening stages. Methods A simple and effective method based on UPLC-ELSD was developed for the simultaneous determination of two monosaccharides（glucose and fructose）, two disaccharides（sucrose and maltose）, one sugar alcohol（xylitol）, and betaine in wolfberry fruits（goji berries） of genus Lycium L. Results The six species of wolfberry fruits harvested at the three different ripening stages were evaluated in sugars and betaine contents. Fructose and glucose were the predominant sugars in mature wolfberry fruits. Fructose, glucose, and betaine, as well as total sugar contents, increased continually over the ripening process. L. truncatum and L. dasystemum had higher contents of sugars and betaine than the other species. Conclusion UPLC-ELSD is a simple, reliable and effective method for analysis of the sweet chemicals in wolfberries. Wolfberry fruits at the different ripening stages were significant different in sweetness. L. truncatum and L. dasystemum could be the potential sources of wolfberry fruit sources.
