Soybean(Glycine max)is short-day(SD)plant.Flowering time is a key agronomic trait that determines the transition from vegetative to reproductive growth.The study on the expression and regulation mechanism of flowering...Soybean(Glycine max)is short-day(SD)plant.Flowering time is a key agronomic trait that determines the transition from vegetative to reproductive growth.The study on the expression and regulation mechanism of flowering time gene in soybean photoperiod control of flowering pathway is particularly theoretically significant for soybean genetic improvement.In this study,a dual-luciferase reporter gene system with the GmFT2a gene promoter as promoter sequence was constructed,and the method of Agrobacterium tumefaciens injection into tobacco leaves was selected to study the effects of long and short days on the activity of the GmFT2a gene promoter.The results of transient expression analysis showed that the GmFT2a promoter was strongly induced under the SD conditions in tobacco.Furthermore,analysis of the GmFT2a promoter sequence revealed several cis-acting elements,including G-Box,Box 4,GT1-motif and TCT-motif by PlantCARE search.It was speculated that these elements might promote the expression of GmFT2a gene in the SDs and played a role in promoting flowering.The results of this study provided a basis for a better understanding of the function of the GmFT2a gene and further exploration of the complex flowering mechanism of soybean.展开更多
FLOWERING LOCUS T (FT),a key regulator of photoperiod pathway,plays a pivotal role in modulating flowering time and growth period-related traits in plants.In this study,we identified 10 FT family genes in soybean and ...FLOWERING LOCUS T (FT),a key regulator of photoperiod pathway,plays a pivotal role in modulating flowering time and growth period-related traits in plants.In this study,we identified 10 FT family genes in soybean and examined their functional divergences through sequence alignment analysis.Phylogenetic and amino acid sequence analysis revealed that the 92nd residue and the Segment B domain (sites 129,131,134,135) are the critical determinants of potential functional divergence and activity variation among Gm FT.Using the Soy Omics website,we detected natural variations in the genomic and promoter regions of Gm FTs,along with numerous haplotypes.By correlating these haplotypes with flowering time,we identified 7 potential KASP markers associated with soybean growth period:Gm FT1a-60297360,Gm FT1b-60311669,Gm FT2a-31695562,Gm FT2b-31739433,Gm FT3a-4106476,Gm FT5a-4078626,and Gm FT5b-37490962.All KASP markers exhibited high selection efficiency (97.56%–100.00%) and reliable genotyping accuracy when validated across 82 soybean varieties spanning multiple maturity groups (MGs).This study systematically elucidates the functional divergence of Gm FTs and develops efficient KASP markers,providing essential technological support for photoperiodadaptive breeding and germplasm innovation.展开更多
基金Supported by Chinese National Natural Science Foundation(32072086,31771820)Heilongjiang Province Natural Science Foundation(C2015009)。
文摘Soybean(Glycine max)is short-day(SD)plant.Flowering time is a key agronomic trait that determines the transition from vegetative to reproductive growth.The study on the expression and regulation mechanism of flowering time gene in soybean photoperiod control of flowering pathway is particularly theoretically significant for soybean genetic improvement.In this study,a dual-luciferase reporter gene system with the GmFT2a gene promoter as promoter sequence was constructed,and the method of Agrobacterium tumefaciens injection into tobacco leaves was selected to study the effects of long and short days on the activity of the GmFT2a gene promoter.The results of transient expression analysis showed that the GmFT2a promoter was strongly induced under the SD conditions in tobacco.Furthermore,analysis of the GmFT2a promoter sequence revealed several cis-acting elements,including G-Box,Box 4,GT1-motif and TCT-motif by PlantCARE search.It was speculated that these elements might promote the expression of GmFT2a gene in the SDs and played a role in promoting flowering.The results of this study provided a basis for a better understanding of the function of the GmFT2a gene and further exploration of the complex flowering mechanism of soybean.
基金supported by the National Key R&D Program of China (2023YFD1201300)the Nanfan Special Project of Chinese Academy of Agricultural Sciences (YBXM2428)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences。
文摘FLOWERING LOCUS T (FT),a key regulator of photoperiod pathway,plays a pivotal role in modulating flowering time and growth period-related traits in plants.In this study,we identified 10 FT family genes in soybean and examined their functional divergences through sequence alignment analysis.Phylogenetic and amino acid sequence analysis revealed that the 92nd residue and the Segment B domain (sites 129,131,134,135) are the critical determinants of potential functional divergence and activity variation among Gm FT.Using the Soy Omics website,we detected natural variations in the genomic and promoter regions of Gm FTs,along with numerous haplotypes.By correlating these haplotypes with flowering time,we identified 7 potential KASP markers associated with soybean growth period:Gm FT1a-60297360,Gm FT1b-60311669,Gm FT2a-31695562,Gm FT2b-31739433,Gm FT3a-4106476,Gm FT5a-4078626,and Gm FT5b-37490962.All KASP markers exhibited high selection efficiency (97.56%–100.00%) and reliable genotyping accuracy when validated across 82 soybean varieties spanning multiple maturity groups (MGs).This study systematically elucidates the functional divergence of Gm FTs and develops efficient KASP markers,providing essential technological support for photoperiodadaptive breeding and germplasm innovation.
