The high molecular weight (HMW) glutenin subunits from 85 varieties, including 22 corner stone breeding parents, 45 widely cultivated varieties in the different period of Chinese wheat production and 18 recent varieti...The high molecular weight (HMW) glutenin subunits from 85 varieties, including 22 corner stone breeding parents, 45 widely cultivated varieties in the different period of Chinese wheat production and 18 recent varieties with good processing quality, were fractionated by SDS PAGE. The 22 corner stone breeding lines were found to carry either null (c) or 1(a) subunits on Glu A1 locus. Five types of subunits were detected for Glu B1 , which were 7+8 (b), 7+9 (c), 14+15 (h), 17+18 (i) and 6+8 (d), with subunit pairs 7+8 and 7+9 being the two major types. Six types of subunits were detected for Glu D1 , which are 2+12 (a), 2+10 (e), 5+10 (d), 4+10 (j), 4+12 (c) and 2+11 (g), with subunit pairs 2+12 and 2+11 being the two major types. With respect to the Glu D1 locus, among the corner stone breeding lines, Early premium from American carried 5+10, St2422/464 from Italy carried the 14+15, whereas two related varieties, Mara and Alondra, carried 5+10. In the 45 commercialized varieties, only Yangmai 5 possessed the 5+10 subunit gene pairs at Glu D1 , Xiaoyan 6 and Yumai 7 possessed the 14+15 subunit gene pairs at Glu B1 . The Glu B1 locus of four varieties, Funo, Nongda 139, Zhengzhou 683 and Fan 6 all specified the 17+18 subunits. The 18 varieties recommended by the National Agricultural Ministry in 1992 as being the ones possessing good processing property could be classified into two groups, one carried the 5+10 subunit pair encoded by the Glu D1 locus, the other carried the 14+15 subunit pair encoded by the Glu B1 locus. Zhongzuo 8131 and its selections possessed the 5+10 subunit pair, the coding genes of which were derived from either Yecora F 70 or IRN68 181. Xiaoyan 6 and its derivative varieties carried the 14+15 subunit pair, the coding genes of which were derived from St2422/464, a variety that was bred in the early part of the 20th century in Italy by N. Strampelli. The 17+18 subunit encoding genes in Chinese varieties were derived from Funo or its selections. These results provided for the first time a basic pattern of allelic changes at the Glu 1 loci in Chinese wheat varieties in the past 50 years, which may have important implications in the improvement of protein quality in Chinese wheat in the future.展开更多
文摘The high molecular weight (HMW) glutenin subunits from 85 varieties, including 22 corner stone breeding parents, 45 widely cultivated varieties in the different period of Chinese wheat production and 18 recent varieties with good processing quality, were fractionated by SDS PAGE. The 22 corner stone breeding lines were found to carry either null (c) or 1(a) subunits on Glu A1 locus. Five types of subunits were detected for Glu B1 , which were 7+8 (b), 7+9 (c), 14+15 (h), 17+18 (i) and 6+8 (d), with subunit pairs 7+8 and 7+9 being the two major types. Six types of subunits were detected for Glu D1 , which are 2+12 (a), 2+10 (e), 5+10 (d), 4+10 (j), 4+12 (c) and 2+11 (g), with subunit pairs 2+12 and 2+11 being the two major types. With respect to the Glu D1 locus, among the corner stone breeding lines, Early premium from American carried 5+10, St2422/464 from Italy carried the 14+15, whereas two related varieties, Mara and Alondra, carried 5+10. In the 45 commercialized varieties, only Yangmai 5 possessed the 5+10 subunit gene pairs at Glu D1 , Xiaoyan 6 and Yumai 7 possessed the 14+15 subunit gene pairs at Glu B1 . The Glu B1 locus of four varieties, Funo, Nongda 139, Zhengzhou 683 and Fan 6 all specified the 17+18 subunits. The 18 varieties recommended by the National Agricultural Ministry in 1992 as being the ones possessing good processing property could be classified into two groups, one carried the 5+10 subunit pair encoded by the Glu D1 locus, the other carried the 14+15 subunit pair encoded by the Glu B1 locus. Zhongzuo 8131 and its selections possessed the 5+10 subunit pair, the coding genes of which were derived from either Yecora F 70 or IRN68 181. Xiaoyan 6 and its derivative varieties carried the 14+15 subunit pair, the coding genes of which were derived from St2422/464, a variety that was bred in the early part of the 20th century in Italy by N. Strampelli. The 17+18 subunit encoding genes in Chinese varieties were derived from Funo or its selections. These results provided for the first time a basic pattern of allelic changes at the Glu 1 loci in Chinese wheat varieties in the past 50 years, which may have important implications in the improvement of protein quality in Chinese wheat in the future.
