Cotton fiber development is a highly programmed process,involving a large number of genes.Through cDNA array and R T-PCR,we isolated a Gossypium hirsutum RD22-1 like gene(GhRDL1),homologous to Arabi dopsis RD22.GhRDL1...Cotton fiber development is a highly programmed process,involving a large number of genes.Through cDNA array and R T-PCR,we isolated a Gossypium hirsutum RD22-1 like gene(GhRDL1),homologous to Arabi dopsis RD22.GhRDL1 was highly expressed in elongating cotton fiber(3~15 DPA).The ORF of RDL1 encodes a protein of 335 amino acids,with a 19-amino acid signal peptide at the N-terminal and a conser ved plant-specific domain,BURP,at the C-terminal.Gene bombardment assay showed that green fluore scent protein(GFP)-RDL1 fusion was localized to the cell wall of onion epidermal cells,a similar location was observed with transgenicArabidopsis expressing GFP-RDL1.展开更多
文摘Cotton fiber development is a highly programmed process,involving a large number of genes.Through cDNA array and R T-PCR,we isolated a Gossypium hirsutum RD22-1 like gene(GhRDL1),homologous to Arabi dopsis RD22.GhRDL1 was highly expressed in elongating cotton fiber(3~15 DPA).The ORF of RDL1 encodes a protein of 335 amino acids,with a 19-amino acid signal peptide at the N-terminal and a conser ved plant-specific domain,BURP,at the C-terminal.Gene bombardment assay showed that green fluore scent protein(GFP)-RDL1 fusion was localized to the cell wall of onion epidermal cells,a similar location was observed with transgenicArabidopsis expressing GFP-RDL1.
