Growth factor independence 1 （GFI1） is important for maturation of mammalian lymphocytes and neutrophils and maintenance of adult hematopoietic stem cells （HSCs）. The role of GFI1 in embryonic hematopoiesis is les...Growth factor independence 1 （GFI1） is important for maturation of mammalian lymphocytes and neutrophils and maintenance of adult hematopoietic stem cells （HSCs）. The role of GFI1 in embryonic hematopoiesis is less well characterized. Through an enhancer trap screen and bioinformatics analysis, we identified a zebrafish homolog of Gill （named grill） and analyzed its function during embryonic development. Expression of both an endogenous griLl gene and a GFP reporter gene inserted near its genomic locus was detected in hematopoietic cells of zebrafish embryos. Morpholino （MO） knockdown of gill.1 reduced expression of scl, Imo2, c-myb, mpo, ragl, gatal and hemoglobin alpha embryonic-1 （hbael）, as well as the total amount of embryonic hemoglobin, but increased expression ofpu.1 and l-plastin. Under the same conditions, MO injection did not affect the markers involved in vascular and pronephric development. Conversely, overexpression of gill.1 via mRNA injection enhanced expression ofgatal but inhibited expression ofpu.1. These findings suggest that Gill.1 plays a critical role in regulating the balance of embryonic erythroid and myeloid lineage determination, and is also required for the differentiation of lymphocytes and granulocytes during zebrafish embryogenesis.展开更多
目的研究地塞米松对小鼠Th17细胞分化模型中IL-17f、gfi-1表达的影响。方法提取小鼠脾脏淋巴细胞,采用免疫磁珠纯化CD4^+CD62L^+T细胞,将其分为对照组、地塞米松组、Th17组、地塞米松组+Th17组。Th17组加antiCD3ε、anti-CD28、anti-IFN...目的研究地塞米松对小鼠Th17细胞分化模型中IL-17f、gfi-1表达的影响。方法提取小鼠脾脏淋巴细胞,采用免疫磁珠纯化CD4^+CD62L^+T细胞,将其分为对照组、地塞米松组、Th17组、地塞米松组+Th17组。Th17组加antiCD3ε、anti-CD28、anti-IFN-γ、anti-IL-4抗体及IL-6、TGF-β1、IL-23,地塞米松+Th17组在Th17组培养条件下加10μg/L地塞米松。细胞培养72 h后,采用Real-time PCR检测4组IL-17f、gfi-1 m RNA表达。结果 Th17组IL-17f m RNA表达明显增高(P<0.01)。与Th17组比较,地塞米松+Th17组IL-17f m RNA表达明显降低(P<0.01),而gfi-1 m RNA表达明显增高(P<0.01)。结论地塞米松可下调IL-17f和上调gfi-1表达,gfi-1表达增加可能引起骨质疏松。展开更多
文摘Growth factor independence 1 （GFI1） is important for maturation of mammalian lymphocytes and neutrophils and maintenance of adult hematopoietic stem cells （HSCs）. The role of GFI1 in embryonic hematopoiesis is less well characterized. Through an enhancer trap screen and bioinformatics analysis, we identified a zebrafish homolog of Gill （named grill） and analyzed its function during embryonic development. Expression of both an endogenous griLl gene and a GFP reporter gene inserted near its genomic locus was detected in hematopoietic cells of zebrafish embryos. Morpholino （MO） knockdown of gill.1 reduced expression of scl, Imo2, c-myb, mpo, ragl, gatal and hemoglobin alpha embryonic-1 （hbael）, as well as the total amount of embryonic hemoglobin, but increased expression ofpu.1 and l-plastin. Under the same conditions, MO injection did not affect the markers involved in vascular and pronephric development. Conversely, overexpression of gill.1 via mRNA injection enhanced expression ofgatal but inhibited expression ofpu.1. These findings suggest that Gill.1 plays a critical role in regulating the balance of embryonic erythroid and myeloid lineage determination, and is also required for the differentiation of lymphocytes and granulocytes during zebrafish embryogenesis.
文摘目的研究地塞米松对小鼠Th17细胞分化模型中IL-17f、gfi-1表达的影响。方法提取小鼠脾脏淋巴细胞,采用免疫磁珠纯化CD4^+CD62L^+T细胞,将其分为对照组、地塞米松组、Th17组、地塞米松组+Th17组。Th17组加antiCD3ε、anti-CD28、anti-IFN-γ、anti-IL-4抗体及IL-6、TGF-β1、IL-23,地塞米松+Th17组在Th17组培养条件下加10μg/L地塞米松。细胞培养72 h后,采用Real-time PCR检测4组IL-17f、gfi-1 m RNA表达。结果 Th17组IL-17f m RNA表达明显增高(P<0.01)。与Th17组比较,地塞米松+Th17组IL-17f m RNA表达明显降低(P<0.01),而gfi-1 m RNA表达明显增高(P<0.01)。结论地塞米松可下调IL-17f和上调gfi-1表达,gfi-1表达增加可能引起骨质疏松。
